BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,252,698	A→G	147 (TAA→TAG)	CXP41_RS06625 →	protein YcgY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,252,698	0	A	G	99.8%	1369.7 / NA	395	147 (TAA→TAG)	CXP41_RS06625	protein YcgY
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (214/180); minor base T (1/0); total (215/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 408 total aligned reads displayed.

TATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGT  >  NZ_CP025268/1252561‑1252833

   aGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTagaaaaa                                                                                                                                    >  2:9711019/1‑140 (MQ=255)

   aGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTagaaaaa                                                                                                                                    >  2:6471752/1‑140 (MQ=255)

   aGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTagaaaaa                                                                                                                                    >  1:3228016/1‑140 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGTTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAATTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTagaaaaacc                                                                                                                                  >  2:9554544/1‑138 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCACACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  >  1:3921201/1‑140 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  >  1:9622260/1‑140 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  >  2:1240341/1‑140 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  <  2:3290138/140‑1 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  >  1:3267575/1‑140 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  >  1:2496412/1‑140 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  <  1:5686992/140‑1 (MQ=255)

     ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  >  1:8571384/1‑140 (MQ=255)

      cAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAcc                                                                                                                                 >  1:9104713/1‑140 (MQ=255)

       aaCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCa                                                                                                                                <  1:6159/140‑1 (MQ=255)

       aaCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCa                                                                                                                                <  1:6436552/140‑1 (MQ=255)

        aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAATACCAt                                                                                                                               >  1:5181960/1‑140 (MQ=255)

        aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               >  1:1809572/1‑140 (MQ=255)

        aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               >  2:3891191/1‑140 (MQ=255)

        aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               <  2:3615198/140‑1 (MQ=255)

        aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               >  1:8911670/1‑140 (MQ=255)

        aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               >  1:3770367/1‑140 (MQ=255)

        aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCAGTGTAGAAAAAACCAt                                                                                                                               >  2:1911742/1‑140 (MQ=255)

          gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             <  1:4904206/140‑1 (MQ=255)

          gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             >  1:3661659/1‑140 (MQ=255)

          gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             >  1:2268681/1‑140 (MQ=255)

          gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             >  2:4358014/1‑140 (MQ=255)

          gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             >  1:10685565/1‑140 (MQ=255)

          gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             >  1:10302164/1‑140 (MQ=255)

            cGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCt                                                                                                                           <  1:1911742/140‑1 (MQ=255)

             ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTa                                                                                                                          >  2:6797343/1‑140 (MQ=255)

             ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTa                                                                                                                          >  1:840372/1‑140 (MQ=255)

               cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATg                                                                                                                        >  1:9472320/1‑140 (MQ=255)

               cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATg                                                                                                                        >  2:8371105/1‑140 (MQ=255)

                         cGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTg                                                                                                              <  1:5994073/140‑1 (MQ=255)

                             tgatgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGa                                                                                                          <  1:9370796/140‑1 (MQ=255)

                               atgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACa                                                                                                        >  2:9822879/1‑140 (MQ=255)

                                 gaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAAc                                                                                                      <  2:1386635/140‑1 (MQ=255)

                                  aaaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                     <  2:8247831/140‑1 (MQ=255)

                                  aaaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                     <  2:901932/140‑1 (MQ=255)

                                  aaaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGACAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                     <  2:8596561/140‑1 (MQ=255)

                                     gTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCa                                                                                                  <  2:10639961/140‑1 (MQ=255)

                                      tGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCa                                                                                                                                <  2:6352341/109‑1 (MQ=255)

                                      tGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCAt                                                                                                 >  2:10288678/1‑140 (MQ=255)

                                       gAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATc                                                                                                >  2:5498510/1‑140 (MQ=255)

                                         aaCACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCt                                                                                                                           >  1:4635326/1‑111 (MQ=255)

                                         aaCACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCt                                                                                                                           <  2:4635326/111‑1 (MQ=255)

                                          acacTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCa                                                                                                                                <  2:8922317/105‑1 (MQ=255)

                                               tACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCg                                                                                                           <  2:469469/121‑1 (MQ=255)

                                               tACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAg                                                                                        <  1:7431948/140‑1 (MQ=255)

                                                aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCt                                                                                                               >  1:1337996/1‑116 (MQ=255)

                                                aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGc                                                                                       >  2:7409188/1‑140 (MQ=255)

                                                aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGc                                                                                       >  1:545073/1‑140 (MQ=255)

                                                   cGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGa                                                                                    >  1:1515662/1‑140 (MQ=255)

                                                     tAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                                  >  2:1172802/1‑140 (MQ=255)

                                                     tAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                                  <  2:2758572/140‑1 (MQ=255)

                                                      aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGgtgt                                                                                                                    <  1:7903310/105‑1 (MQ=255)

                                                      aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATg                                                                                 >  2:8518334/1‑140 (MQ=255)

                                                      aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATg                                                                                 >  2:2228978/1‑140 (MQ=255)

                                                       gAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                                                <  1:8727956/140‑1 (MQ=255)

                                                       gAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                                                <  2:2339677/140‑1 (MQ=255)

                                                         aaTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                                              <  2:8304630/140‑1 (MQ=255)

                                                          aTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGt                                                                             >  2:10085135/1‑140 (MQ=255)

                                                           tGACGCTATCTTCGGCAACCACGTATGGCAATCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTCTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGtt                                                                            >  2:9260785/1‑140 (MQ=255)

                                                            gACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTg                                                                           >  2:1507598/1‑140 (MQ=255)

                                                                cTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGa                                                                                    >  2:5799575/1‑127 (MQ=255)

                                                                cTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGACGACAACGCCATCCGGAGAAGCCGCATGTCGTTGTCgg                                                                       >  2:10437112/1‑140 (MQ=255)

                                                                cTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACGTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCGGCCGACCACGCCATCCGGAGAAGCCGAATGACGTTGTCgg                                                                       >  2:6447131/1‑140 (MQ=255)

                                                                       cGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                                <  1:6961247/140‑1 (MQ=255)

                                                                       cGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                                <  1:7291194/140‑1 (MQ=255)

                                                                        ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcg                                                               >  1:6287052/1‑140 (MQ=255)

                                                                            aCCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTgg                                                           <  1:3086747/140‑1 (MQ=255)

                                                                             ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGt                                                          >  1:3394498/1‑140 (MQ=255)

                                                                             ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGt                                                          >  2:3020853/1‑140 (MQ=255)

                                                                             ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGAAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGt                                                          >  2:7994048/1‑140 (MQ=255)

                                                                              cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  1:4358014/140‑1 (MQ=255)

                                                                              cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  1:9884386/140‑1 (MQ=255)

                                                                              cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  1:3877939/140‑1 (MQ=255)

                                                                              cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  1:1240341/140‑1 (MQ=255)

                                                                                       cAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTgg                                                >  2:1648300/1‑140 (MQ=255)

                                                                                         aaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGt                                              >  1:10002242/1‑140 (MQ=255)

                                                                                          aCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGtt                                             >  1:10254103/1‑140 (MQ=255)

                                                                                           cATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCGGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACATCGCCATCCGGAGAAGCCGACTGACGTTGTCGGAGTAAGCGCTGGTTATGGTGTGGGTtg                                            >  2:7693485/1‑140 (MQ=255)

                                                                                            aTTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtgt                                           <  1:7645962/140‑1 (MQ=255)

                                                                                                gcgcAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCct                                       <  2:6029958/140‑1 (MQ=255)

                                                                                                gcgcAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCct                                       <  2:481960/140‑1 (MQ=255)

                                                                                                  gcAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                                     <  1:10476031/140‑1 (MQ=255)

                                                                                                  gcAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                                     <  1:6355443/140‑1 (MQ=255)

                                                                                                      tACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTGTgg                                 >  2:6860573/1‑140 (MQ=255)

                                                                                                          tCCCGCCAGGACTTGAAAAACCGTCTGTGTAGTAAAAACCATAGCTAGGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTCAGCGCTGGTTAAGGTGTGGGTTGTGACTCTTTGGTTGa                             >  2:10829593/1‑140 (MQ=255)

                                                                                                           cccGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTAAAGGTGTGGGTTGTGCCTCTTTGGTTGAg                            >  1:395161/1‑140 (MQ=255)

                                                                                                              gCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                                                <  1:7765271/85‑1 (MQ=255)

                                                                                                                cAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCt                                                                                                                           <  1:10841574/40‑1 (MQ=255)

                                                                                                                cAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTg                       >  1:8136141/1‑140 (MQ=255)

                                                                                                                   gACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcgt                    >  1:2946041/1‑140 (MQ=255)

                                                                                                                    aCTTGAAAAACCGTCTGAGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcgtc                   <  2:6990629/140‑1 (MQ=255)

                                                                                                                          aaaaCCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCt             <  2:6730442/140‑1 (MQ=255)

                                                                                                                                gTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTa       >  1:6709232/1‑140 (MQ=255)

                                                                                                                                 tCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTaa      <  2:5180601/140‑1 (MQ=255)

                                                                                                                                   tgtgTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTa                                                        >  2:7127846/1‑88 (MQ=255)

                                                                                                                                   tgtgTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTa                                                        <  1:7127847/88‑1 (MQ=255)

TATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGT  >  NZ_CP025268/1252561‑1252833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`