BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,160	G→A	noncoding (55/132 nt)	CXP41_RS06840 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,160	0	G	A	90.4%	246.7 / 14.7	127	noncoding (55/132 nt)	CXP41_RS06840	RtT sRNA
Reads supporting (aligned to +/- strand): ref base G (8/3); new base A (72/32); total (87/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

Only 100 of 127 total aligned reads displayed.

GTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAT  >  NZ_CP025268/1293061‑1293297

gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTACGCCCATCTGGTCGTTGCCAGCGGCAACGCTCTCAAGCTGGCGCTCTAGTCGAAACTTGGTCGAAGCTTAACAACC‑TTccc                                                                                                   >  1:9077593/1‑140 (MQ=11)

                                          cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTTCGGCAACGCACACACGCATGCGCACAAGTCGAACCATGGTCGAAGAGTCTCAACC‑ATCCCCCCATGGGCACAAAATTTGATTGCGGATTCGTTTgataaatc                                                         >  1:6038604/1‑134 (MQ=0)

                                          cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCATAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGATTTTTTGATTGCGGATTGGGTTGAGAGTTc                                                         >  2:5164141/1‑140 (MQ=11)

                                          cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCAACC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                         >  2:6524204/1‑140 (MQ=255)

                                          cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCC‑ATCCCCCCAAGGGCCGAAAATTTTATTGCGGATTCGGTTGGGAGttg                                                         >  1:5595843/1‑139 (MQ=11)

                                            cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                       <  1:7324784/140‑1 (MQ=18)

                                             aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                      >  1:9941695/1‑140 (MQ=21)

                                              gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACTCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                     <  1:8923211/140‑1 (MQ=255)

                                              gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                     <  2:4950772/140‑1 (MQ=21)

                                              gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                     <  1:4954034/140‑1 (MQ=21)

                                              gagcTGCGCGCTTCGCCGTTCGGGTATTTGCCTTCGTCAAAGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                     <  1:5605242/138‑1 (MQ=11)

                                               cgcTGCGCGCTTCGCCCTTCGGTTCGTTGCATGCTGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                    <  2:8502214/140‑1 (MQ=11)

                                               cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCAAGACGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                    >  2:9447653/1‑140 (MQ=255)

                                               cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGGGCCGAACCGTTGTCGAAGCGTCTCATCC‑TTCCCCGCATGGGCCGAAGATTTGATTGCGGGTTCGCGTGGGAGTTGAgggg                                                    >  1:9628065/1‑139 (MQ=11)

                                               cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATAC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGTGAGTTCAGGGa                                                    >  2:3847413/1‑140 (MQ=11)

                                               cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTAAGGGa                                                    >  2:9389281/1‑140 (MQ=255)

                                               cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCGCGAGGCGAACCTTAGTCGAAGCTTCTCAACC‑TTCCCCGCATGGGCCGAATATTGGATTGCGGGATCGCTGGAGAGGTCAgggc                                                    >  1:800559/1‑139 (MQ=11)

                                               cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCTCGCGGCCGCTCGAGCCGCACCGTAGTCGAAGCGTCTCATCC‑ATCCCCCCAAGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAgggg                                                    >  1:5446821/1‑139 (MQ=11)

                                                gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGAGAGTTCAGGGAc                                                   >  2:203430/1‑140 (MQ=255)

                                                gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAc                                                   >  1:4649291/1‑140 (MQ=255)

                                                gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAc                                                   <  2:921580/140‑1 (MQ=255)

                                                    cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGACGCTCGAGGCGATCCTTAGTCGAAGCTTCTCATCC‑CTGCCCGCATGGGCAGGAGATTTGATTGGGGATTCGCGGGCGAGTTCAGGGACgttt                                               >  2:1469081/1‑140 (MQ=11)

                                                    cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCC‑TTCCCCGCCTGGGCCGAATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGACgttt                                               >  1:8673294/1‑140 (MQ=11)

                                                    cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGACGCTCGAGCCGAAGCGGAGTCGATGCATCTCCACC‑TTCCCCGCCGGGGCGGAAGAGTTGGTGGCGGATTCGCTTGTGAGGTCAGGGTCtggg                                               >  2:9897670/1‑137 (MQ=2)

                                                       gcggcGCCCCTCGGTTCGGTGCCTGCCGCACCGCTCTCTCGCTGACGCTCGAGCCGACCCCTCGTCGAAGCGTCTCCTCC‑TTCCCCGCATGGGCAGCATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGaa                                            <  2:10316824/136‑1 (MQ=11)

                                                       gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                            <  1:9447654/140‑1 (MQ=255)

                                                       gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                            <  2:2599241/140‑1 (MQ=255)

                                                       gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                            <  2:288454/140‑1 (MQ=255)

                                                       gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                            <  1:7925691/140‑1 (MQ=255)

                                                       gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACATTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                            <  2:8229651/140‑1 (MQ=255)

                                                        cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                           <  2:7507210/140‑1 (MQ=255)

                                                        cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                           <  2:7626097/140‑1 (MQ=255)

                                                         ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCAAGGCGAACCTTTGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                          <  2:5489478/140‑1 (MQ=11)

                                                         ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                          >  2:4482347/1‑140 (MQ=255)

                                                           cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                        <  2:7515880‑M2/140‑2 (MQ=255)

                                                           cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                        >  1:3646550‑M2/1‑139 (MQ=255)

                                                           cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                        >  2:5805950‑M2/1‑139 (MQ=255)

                                                            gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                       >  1:2028266‑M2/1‑138 (MQ=255)

                                                            gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                       >  2:10066030‑M2/1‑138 (MQ=255)

                                                             cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgat                                      >  1:7669661‑M2/1‑137 (MQ=255)

                                                             cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgat                                      >  2:2588026‑M2/1‑137 (MQ=255)

                                                             cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgat                                      >  2:4249938‑M2/1‑137 (MQ=255)

                                                              ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                     >  1:7515881‑M2/1‑136 (MQ=255)

                                                              ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                     >  1:1752389‑M2/1‑136 (MQ=255)

                                                              ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                     >  1:6590191‑M2/1‑136 (MQ=255)

                                                              ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATGGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                     >  1:10828234‑M2/1‑136 (MQ=255)

                                                               cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatgg                                    >  1:8209411‑M2/1‑135 (MQ=255)

                                                                ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggt                                   >  2:9837897‑M2/1‑134 (MQ=255)

                                                                ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggt                                   <  2:3407356‑M2/140‑7 (MQ=255)

                                                                 tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCGCGAGCCGAACCTTAGTCGAAGCGTCTCATCC‑GTCCCCGCCTGGGCCGAATATTTGATTGCGGATTCGCGTGTGAGTTCAGGGACTTTTGTAAGTgatggtg                                  <  1:1804920‑M2/140‑8 (MQ=255)

                                                                  cGGGTCGTTGCCTGCTGCTCCGCACTCTCCCACACGCTCGTTCCGAACCTTAGTCGAACCTTCTCATCC‑TTCCCCTCATGGGATGAATAATTGATTGCTGATTCGCTTTAGAGTTCTGGGACTTTTGAAAGTGAtggtgt                                 <  2:8893621/140‑2 (MQ=9)

                                                                  cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                 >  2:4649291‑M2/1‑132 (MQ=255)

                                                                   gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTgatggtggt                                >  1:8866939‑M2/1‑131 (MQ=255)

                                                                   gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                >  1:7361230‑M2/1‑131 (MQ=255)

                                                                   gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                >  1:5474503‑M2/1‑131 (MQ=255)

                                                                   gggTCGTTGCCTGCGGCAACGCTCTCGCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                >  1:5949640‑M2/1‑131 (MQ=255)

                                                                   gggTCGTTGCCGGCGGCTACGCTCTCTCGCTGACGCTCGAGCCGAACCTGAGTCGACGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGAGTGCGGAGTCGCTGGAGAGTTCAGGGACTTGTGAAAGTgatggtggt                                >  2:10196832‑M2/1‑131 (MQ=255)

                                                                   gggTCGTTGCCAGCGGCAACGCTCTCACGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑GTCCCCGCCAGGGCAGAATATTTGATTTCGGATTCGCTTGATAGTTCAGGGACTTTTGAAAGTGAtggtggt                                >  1:7379710/1‑140 (MQ=11)

                                                                    ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtg                               >  1:2864320‑M2/1‑130 (MQ=255)

                                                                     gTCGTTGCCTGCGGCCACGCTCTCTCGCTGACGCGCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑ATCCCCGCATGGGCCGAATATTTTATTGCGGATTCGCTTGAGAGTTCAGGGACGGTTGAAAGAGAtggtggtgg                              >  1:1654086/1‑140 (MQ=11)

                                                                     gTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTGTGTGAGTTCAGGGTCGTTTGAAAGTGAgggtggggg                              >  2:8052684/1‑140 (MQ=11)

                                                                      tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCTAGCAGTACCTTAGTCGAAGCTTCTCATCC‑TTCCCAGCATGGGCAGAATATTTGATTGCGGTTTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGTTggg                             <  2:6746794/140‑1 (MQ=11)

                                                                      gcGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCGTCGCCTCC‑TTCCCCGCATGGGCAGACTATATGATTGCGGATTCGCTTGAGCGTTCAGGGACTTTTGAACGTGATGGTGGTggg                             <  2:3960879/139‑1 (MQ=11)

                                                                        gtcgCCAGCGGCAACGCACTCTCGCCGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATGC‑TTCCCCGAATGGGCAGTTTTTTTGTTTTCGTTGTCTCTTTTTTGTTCATTTAATTTTGAAAGTGATGGTGGTggggg                           <  1:4896588/137‑1 (MQ=2)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCTGGGACTTTTGAAAGTgatggtggtggggg                           >  1:7417670‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCC‑TTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                            <  1:9491294/123‑1 (MQ=11)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGCAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:840304‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:744567‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:10677605‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:10776126‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:3120717‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:7417691‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:8148085‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATATGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:7563941‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCCAGACGAACCTTTGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGATTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:7912569‑M2/1‑126 (MQ=255)

                                                                        gTTGCCTGCGGCAACGCGCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGACTAGTTGATTGCGGATTAGCTTGAGATTTCATGGACTTTTGTAAGTgatggtggtggggg                           >  2:10704137‑M2/1‑126 (MQ=255)

                                                                         ttGCCTGCGGCAAAGCTCTCTCGCTGTCGCTCGAGCCGAACCGTAGTCGAAGCGTCTCATCC‑TTCCCCGCATGGGCAGAATATTTTAGTGCGGATTCGCTGGCGAGTTCAGGGACTTTTGAAAGTgatggtgggggggga                          <  2:2752808‑M2/140‑16 (MQ=255)

                                                                          tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCATGGCCCGAAT‑TGCACAATTTATGATTGCGGATAATCATGTGAGGTCTGGGACTTTTGTAAGTGAGGGTGGTGGGGGat                         >  2:3404482/1‑139 (MQ=2)

                                                                          tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCAGGGGCCGTATATGTGCGTGCGGCTTCGGTTGAGAGGGCTGGGACTTGTGAAAGTGATGGTGGTGGGGGag                         >  2:7410038/1‑139 (MQ=11)

                                                                          tGCCAGCCGCAACGCTCTCTCGCTGACGCTCGAGCCCAAACTTAGTCGAAGCTTCACATCC‑TTCCCCCCAAGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaa                         <  2:3403977‑M2/140‑17 (MQ=255)

                                                                           gCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCGTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaag                        >  2:1655113‑M2/1‑123 (MQ=255)

                                                                            ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagg                       >  2:541997‑M2/1‑122 (MQ=255)

                                                                             cTGCGTCAACGCTCTCTCTCTGACGCTCGAGCCGAAACTTAGTCGTATCTTATCATCC‑TTCCCCGCATTGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgcgggaagga                      >  1:5825229‑M2/1‑121 (MQ=255)

                                                                             cTGCGGCTACGCTCTCTCTCTGACGCTCGAGCCGACCCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCTTGGGCAGATTATTTGATTGCGGCTTCGATAGAGTTTTCATGGAATTTTGTAAGTGATGGTGGTGGGGGAAGGa                      <  1:3801046/140‑1 (MQ=11)

                                                                             cTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAACC‑TTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  2:6810421‑M2/1‑121 (MQ=255)

                                                                             cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  2:5969771‑M2/1‑121 (MQ=255)

                                                                             cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGTAAGTgatggtggtgggggaagga                      >  2:9160086‑M2/1‑121 (MQ=255)

                                                                             cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  1:902482‑M2/1‑121 (MQ=255)

                                                                             cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTACCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  2:9757256‑M2/1‑121 (MQ=255)

                                                                             cTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTTGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  1:10295380‑M2/1‑121 (MQ=255)

                                                                              tGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTgatggtggggggggcagtaa                     <  2:4007072‑M2/140‑21 (MQ=255)

                                                                               gCGGCAACGCTCTCTCGCTGACGCTCCAGCCGAACCTTAGTCGAAGCTTCTCAACC‑GTCCCCGCCTGGGCAGAATATTTGATTGCGGATTCGCTTGGTAGTTAAAGGACTTTTGAAAGTGATGGTGGGGGGGGGAGGGtt                    >  2:9230673/1‑140 (MQ=11)

                                                                                    aaCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactca               >  1:4063970‑M2/1‑114 (MQ=255)

                                                                                    aaCGCTCTCACGCTGACGCTCGAGCCGAACCTGAGTCGCAGCGTCTCATCC‑TGCCCCGCATGGGCAGAATCTTTGATTGCGGATGCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactca               >  2:6930636‑M2/1‑114 (MQ=255)

                                                                                     aCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcag              >  1:2692951‑M2/1‑113 (MQ=255)

                                                                                      cGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactctgc             <  2:6929219‑M2/140‑29 (MQ=255)

                                                                                         tctctcGCTGGCGCTCGAGGCGAACCTTTGTCGTTGATTGTCATCG‑GTGACAGATTGTGAAGAATATTTGATTGCGGATTCGTTGGGAATTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg          <  2:1220165/140‑1 (MQ=2)

                                                                                          ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctg         >  1:3407356‑M2/1‑108 (MQ=255)

                                                                                                cTGACGCTCGAGCCGAACCTTAGTCGAAGCGTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGAGTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgct   >  1:5153436‑M2/1‑102 (MQ=255)

                                                                                                 tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCC‑TTCCCCGCAAGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcatcgctgcgcgcgt  <  2:1081233‑M2/140‑40 (MQ=255)

GTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCC‑TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAT  >  NZ_CP025268/1293061‑1293297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`