Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,786,781 G→C G190G (GGG→GGC ydiS → FAD‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,786,7810GC99.9% 2473.2 / NA 710G190G (GGG→GGCydiSFAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (227/481);  minor base A (1/0);  total (228/482)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 711 total aligned reads displayed.

CAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATCCTTG  >  NZ_CP025268/1786644‑1786916
                                                                                                                                         |                                                                                                                                       
cAGGCTGGTGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:7639130/140‑1 (MQ=255)
cAGGCTGGGGATGTTTGGCTCGAAGCGACTGTGGTGATTCTGGCTGATGGCGTTAACTCGGTGCTTGTCCGCTGGCGGGGAATTGGTCCCGCTTCCCAGCCGGATCTGTACGGTGTTGGTGTTAAAGAGGGTATTGGGCt                                                                                                                                       <  2:9560149/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCNGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  1:10058518/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  1:7101916/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  1:4672803/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:10146522/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  1:9900938/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  1:365920/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:358153/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  1:3343886/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  1:2913906/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:6800426/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  1:5334745/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:9753391/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCAGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:6976979/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGAGTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:3362501/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCGCGAAGCGAATGTGGTGCTTCGGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGGTGGTGATAAAGAGGTTATTGGCCt                                                                                                                                       <  2:9281124/140‑1 (MQ=255)
cAGGCTGGGGATGATATTCCCGACGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTGGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:6735987/140‑1 (MQ=255)
cAGGCTGGGGATGATAGTCTCGAAGCGAATGTGGTGATTCTGGCAGATGGCGTTAACTCGCTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                                                                                                                                       <  2:6374694/140‑1 (MQ=255)
  ggCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTca                                                                                                                                     <  2:65535/140‑1 (MQ=255)
     tGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCacac                                                                                                                                  >  2:4332376/1‑140 (MQ=255)
     tGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCacac                                                                                                                                  >  1:8354749/1‑140 (MQ=255)
     tGGGGATGATATTCTCGAAGCGAATGTGGTGAGTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCGGTTGGTGTTAAAGAGGTTATTGGCCTCacac                                                                                                                                  >  2:3713132/1‑140 (MQ=255)
      ggggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACAcc                                                                                                                                 >  1:1354454/1‑140 (MQ=255)
          ttgatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa                                                                                                                             <  1:553190/139‑1 (MQ=255)
          ctgatATTCTCGAAGCGAATGTGGTGATTCTGGCAGATGGCGGTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTGGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa                                                                                                                             <  2:177691/139‑1 (MQ=255)
          atgatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGTGGTTTTTGGCCTCACACCAGaa                                                                                                                             <  2:6096878/140‑1 (MQ=255)
          atgatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa                                                                                                                             <  2:1187652/140‑1 (MQ=255)
          atgatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa                                                                                                                             <  1:6007100/140‑1 (MQ=255)
            gatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCGCACACCAGAACa                                                                                                                           <  2:4624640/140‑1 (MQ=255)
             atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAg                                                                                                                          >  1:6738081/1‑140 (MQ=255)
             atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAg                                                                                                                          >  1:8130719/1‑140 (MQ=255)
               atTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGAt                                                                                                                        <  1:5083199/140‑1 (MQ=255)
                 tctcGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCa                                                                                                                      >  1:4817276/1‑140 (MQ=255)
                 tctcGAACCGAATGTGGTGATTCTGGGTGTTGGCGTTAACTCGATGCTTGGCCGCTCGCGGGGTGTGGTTCCCGCGTCCGTGCCGCATCCTTACGCTGTTGGGGGTATTGTGGGGATTGGCCTCGCTCCAGTTCAGATcg                                                                                                                      >  2:4030394/1‑139 (MQ=255)
                   tcGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAAc                                                                                                                    >  2:8002582/1‑140 (MQ=255)
                     gAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGa                                                                                                                  <  2:4132902/140‑1 (MQ=255)
                      aaGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt                                                                                                                 <  2:5743146/140‑1 (MQ=255)
                      aaGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt                                                                                                                 <  1:4931165/140‑1 (MQ=255)
                      aaGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt                                                                                                                 <  2:8539538/140‑1 (MQ=255)
                          gattgtgGTGTTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGTATGGTTCCCGCTTCCGATCCGCATGATTACGCTGTTGGTGTTTAAGAGGTTTTTGGCCTCACACCAGAACAGATCTACGATCGCt                                                                                                             <  2:9046705/137‑1 (MQ=255)
                            aTGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCttt                                                                                                           <  2:5828232/140‑1 (MQ=255)
                            aTGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCttt                                                                                                           <  2:7314876/140‑1 (MQ=255)
                             tgtgGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTa                                                                                                          >  1:1865312/1‑140 (MQ=255)
                                  tgATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATAtt                                                                                                     <  1:10674560/140‑1 (MQ=255)
                                   gATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTCTTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                                    >  2:2893967/1‑140 (MQ=255)
                                   gATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                                    <  2:5616185/140‑1 (MQ=255)
                                   gATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGAATCATTACGCTGTTGGTGTTAAAGAGGTGATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAAGATTa                                                                                                    <  2:4966341/140‑1 (MQ=255)
                                              aTGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGaa                                                                                         >  2:208698/1‑140 (MQ=255)
                                               tGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAg                                                                                        >  1:10234167/1‑140 (MQ=255)
                                                cgccttAACTCGAGGCTAGGCCGCTCCCTGGGAATGCTTCCCGCTTCCGATCCGCAGCTTTACGCTGTTGGTCTTAATGAGGTAATTGGCCTCACACCAGACCAGATCAACGATCGCTTTAATATTACCGGCGACGAAgg                                                                                       <  2:324719/136‑1 (MQ=255)
                                                  cGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTg                                                                                     <  1:4245354/140‑1 (MQ=255)
                                                    ttAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGTGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACTGGCGAGGAAGGTgcc                                                                                   >  2:2347096/1‑140 (MQ=255)
                                                            aTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCt                                                                           >  1:1121361/1‑140 (MQ=255)
                                                                       ctcgctGGGAATGGTTCCCGNTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACNGGCGAGGNAGGTGCCGCCTGGCTGTTTGCCGGtt                                                                >  1:5745483/1‑140 (MQ=255)
                                                                       ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCg                                                                                                               <  1:105140/93‑1 (MQ=255)
                                                                       ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGtt                                                                >  2:10465354/1‑140 (MQ=255)
                                                                       ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGtt                                                                >  1:2119241/1‑140 (MQ=255)
                                                                          gctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTccc                                                             <  2:309090/140‑1 (MQ=255)
                                                                           ctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTGGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTcccc                                                            <  2:1072247/140‑1 (MQ=255)
                                                                                aaTGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTg                                                       <  2:227126/140‑1 (MQ=255)
                                                                                aaTGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTg                                                       <  2:1880330/140‑1 (MQ=255)
                                                                                aaTGGTTCCCGCGTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTg                                                       <  1:8616537/140‑1 (MQ=255)
                                                                                 aTGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGa                                                      <  1:8191944/140‑1 (MQ=255)
                                                                                 aTGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGa                                                      <  1:6521635/140‑1 (MQ=255)
                                                                                  tGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGAc                                                     <  2:4477762/140‑1 (MQ=255)
                                                                                        ccGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTg                                               >  1:2390509/1‑140 (MQ=255)
                                                                                        ccGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAAGATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTg                                               <  2:8933033/140‑1 (MQ=255)
                                                                                          gCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGCTCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGAt                                             >  2:4799684/1‑140 (MQ=255)
                                                                                               cGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCg                                        >  1:3891273/1‑140 (MQ=255)
                                                                                               cGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCg                                        >  1:3438065/1‑140 (MQ=255)
                                                                                                 aTCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCggg                                      >  2:1503586/1‑140 (MQ=255)
                                                                                                    cGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                                   <  1:2753959/140‑1 (MQ=255)
                                                                                                    cGCATCAGTACGCTGTTGGTGTTAAAGAGGTTATAGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                                   <  2:10586554/140‑1 (MQ=255)
                                                                                                     gCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                                  <  1:9465835/140‑1 (MQ=255)
                                                                                                      catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                                   >  2:7806480/1‑138 (MQ=255)
                                                                                                      catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                                   >  1:10410868/1‑138 (MQ=255)
                                                                                                      catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAtt                                 >  2:5517755/1‑140 (MQ=255)
                                                                                                            tACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCta                           <  1:691411/140‑1 (MQ=255)
                                                                                                            tACGCTGTTGGTGTTAAAGAGGGTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTGCTGACGGCCTGATGGGCGGGGGATTTCTCta                           >  1:7356608/1‑140 (MQ=255)
                                                                                                                   ttGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGc                                                                                               >  1:8003374/1‑65 (MQ=255)
                                                                                                                     ggTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCttt                                                                                                           >  2:2804966/1‑51 (MQ=255)
                                                                                                                     ggTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaacaa                  >  2:2595460/1‑140 (MQ=255)
                                                                                                                     ggTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaacaa                  >  2:8651477/1‑140 (MQ=255)
                                                                                                                      gtgtTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAg                 <  1:8783444/140‑1 (MQ=255)
                                                                                                                      gtgtTAAAGAGGTTAGTGGCCTCACACCAGAACAGATCTACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGTTGGGCGGGGGATTTCTCTATACCAACAAg                 <  2:5139734/140‑1 (MQ=255)
                                                                                                                       tgtTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAgg                <  2:540640/140‑1 (MQ=255)
                                                                                                                             agagGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGa                                              <  1:3023189/104‑1 (MQ=255)
                                                                                                                             agagGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCa          <  1:1612811/140‑1 (MQ=255)
                                                                                                                             agagGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCa          >  1:7697153/1‑140 (MQ=255)
                                                                                                                             agagGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCa          >  2:1267428/1‑140 (MQ=255)
                                                                                                                              gagGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCat         <  1:4412044/140‑1 (MQ=255)
                                                                                                                              gagGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCat         <  1:6371286/140‑1 (MQ=255)
                                                                                                                                 gTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                                    <  1:9067368/46‑1 (MQ=255)
                                                                                                                                 gTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                                  <  1:1687741/112‑1 (MQ=255)
                                                                                                                                 gTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATc      >  2:3156945/1‑140 (MQ=255)
                                                                                                                                 gTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTAGACCAACAAGGATTCCATATc      >  2:7133496/1‑140 (MQ=255)
                                                                                                                                   tATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATCCt    >  2:8217099/1‑140 (MQ=255)
                                                                                                                                   tATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATCCt    <  1:4448149/140‑1 (MQ=255)
                                                                                                                                     ttGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATCCTTg  <  2:6258553/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATCCTTG  >  NZ_CP025268/1786644‑1786916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: