Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 183,046 T→C G251G (GGT→GGC)  cdaR → DNA‑binding transcriptional regulator CdaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268183,0460TC98.5% 1998.3 / NA 588G251G (GGT→GGC) cdaRDNA‑binding transcriptional regulator CdaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (179/400);  minor base A (1/8);  total (180/408)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 588 total aligned reads displayed.

CTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTGTGTTACTCGAC  >  NZ_CP025268/182909‑183181
                                                                                                                                         |                                                                                                                                       
cTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCaa                                                                                                                                       <  1:7428642/140‑1 (MQ=255)
   aCCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTa                                                                                                                                    >  2:2592411/1‑140 (MQ=255)
   aCCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTa                                                                                                                                    <  2:2789627/140‑1 (MQ=255)
   aCCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTa                                                                                                                                    <  1:9413921/140‑1 (MQ=255)
       aaaTGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTAtttt                                                                                                                                >  2:8110224/1‑140 (MQ=255)
        aaTGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTa                                                                                                                               <  1:4377323/140‑1 (MQ=255)
         ctgggggTGTTGATACCGGCGATGAACGCAAGTGGGCGCTGGGATGCAGAAGATCCACGACAGCGAGTTGACCACCTGCTTCCCCGCTTGAAAGCGTACGGCCAGCTGCGATTTCGCGTGTCACTGGGCCACTAttttcc                                                                                                                              <  2:10435469/139‑3 (MQ=255)
         acggtggaGTGGCACCCGGCGTTGAACTCCTTGTGGCGCACGGATGCAGAAGATCATCGTAAGCGAGTTGAACACCTGATTACCCGCATGAACGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTAc                                                                                                                              <  2:10094325/138‑1 (MQ=255)
         aTGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTAc                                                                                                                              <  2:7603660/140‑1 (MQ=255)
         aTGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTAc                                                                                                                              <  1:5620468/140‑1 (MQ=255)
         aTGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTAc                                                                                                                              <  2:4359085/140‑1 (MQ=255)
         aTGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTAc                                                                                                                              <  2:3938665/140‑1 (MQ=255)
          tggtggTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGCTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTAcc                                                                                                                             <  2:85337/140‑1 (MQ=255)
          tggtggTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTAcc                                                                                                                             <  1:1346497/140‑1 (MQ=255)
             tggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACATCTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGt                                                                                                                          <  2:523805/140‑1 (MQ=255)
             tggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGt                                                                                                                          <  1:6759538/140‑1 (MQ=255)
             tggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGt                                                                                                                          <  2:74080/140‑1 (MQ=255)
             tggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGt                                                                                                                          <  2:622049/140‑1 (MQ=255)
             tggtgTTGAAACCGGCGTAGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTCTCGCGTTTCACTGGGCAACTATTTTACCGGt                                                                                                                          <  1:1202697/140‑1 (MQ=255)
             aggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGt                                                                                                                          <  1:1148012/139‑1 (MQ=255)
              ggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTc                                                                                                                         >  1:170847/1‑140 (MQ=255)
               gtgtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTAc                                                                                                                              <  1:3933748/134‑1 (MQ=255)
               gtgtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTcc                                                                                                                        >  2:2960240/1‑140 (MQ=255)
                 gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTg                                                                                                                      <  1:6331944/140‑1 (MQ=255)
                 gtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTg                                                                                                                      <  1:9853243/140‑1 (MQ=255)
                  ttGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTgg                                                                                                                     <  1:10668308/140‑1 (MQ=255)
                  ttGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTgg                                                                                                                     <  2:576638/140‑1 (MQ=255)
                  ttGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTgg                                                                                                                     <  1:2319370/140‑1 (MQ=255)
                     aaaCCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAg                                                                                                                  >  1:9090407/1‑140 (MQ=255)
                       aCCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTa                                                                                                                <  1:8851086/140‑1 (MQ=255)
                          ggCGTTGAACTCTTTGGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTg                                                                                                             <  1:5039400/140‑1 (MQ=255)
                           gCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGc                                                                                                            >  1:6987745/1‑140 (MQ=255)
                            cGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                           <  1:473629/140‑1 (MQ=255)
                            cGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                           <  1:6134401/140‑1 (MQ=255)
                            cGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                           <  1:4166369/140‑1 (MQ=255)
                            cGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                           <  2:9147630/140‑1 (MQ=255)
                            cGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                           <  2:6372301/140‑1 (MQ=255)
                            cGTTGAACTCTTCGGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCATCTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                           <  2:2236005/140‑1 (MQ=255)
                                 aaCTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATc                                                                                                      <  2:9004796/140‑1 (MQ=255)
                                   ctctTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCt                                                                                                    <  1:10529219/140‑1 (MQ=255)
                                           ggCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACg                                                                                            <  1:4023772/140‑1 (MQ=255)
                                           ggCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACg                                                                                            >  1:5864019/1‑140 (MQ=255)
                                             cgcTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTCTCGTACGGc                                                                                          >  2:6846459/1‑140 (MQ=255)
                                                tGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGaa                                                                                       >  1:5181627/1‑140 (MQ=255)
                                                   gATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAac                                                                                    <  2:2352802/140‑1 (MQ=255)
                                                     tGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacga                                                                                  <  1:9689023/140‑1 (MQ=255)
                                                       cAGAAGATCATCGTAAGCGAGTTGAACACCTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCATGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                                                <  2:107840/140‑1 (MQ=255)
                                                       cAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                                                >  2:1104126/1‑140 (MQ=255)
                                                            gaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggt                                                                           <  1:7157582/140‑1 (MQ=255)
                                                            gaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggt                                                                           >  2:7802836/1‑140 (MQ=255)
                                                            gaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAgggt                                                                           >  2:6706885/1‑140 (MQ=255)
                                                              tcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtgg                                                                         >  2:6615196/1‑140 (MQ=255)
                                                              tcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtgg                                                                         <  1:8132844/140‑1 (MQ=255)
                                                               catcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggt                                                                        >  1:4444708/1‑140 (MQ=255)
                                                                  cGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTggg                                                                     >  1:6461302/1‑140 (MQ=255)
                                                                      aGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTaaa                                                                 <  2:955055/140‑1 (MQ=255)
                                                                            ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCgg                                                           >  1:1270904/1‑140 (MQ=255)
                                                                            ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCgg                                                           >  2:4172924/1‑140 (MQ=255)
                                                                             tGACCAACTGATTACCCGCAGGAAAGAGTCCGGCCAGCAGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                                          <  2:5839386/140‑1 (MQ=255)
                                                                             tGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                                          <  1:4894332/140‑1 (MQ=255)
                                                                             tGAACAACTGATTACCCGCATGAAAGAGTACGGCAAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                                          <  1:9599720/140‑1 (MQ=255)
                                                                                    cTGATTACCCGCATGAAAGAGTACGGNCAGCTGCNTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTNGGTAAACAGCGGATGCCAGa                                                   <  1:5737624/140‑1 (MQ=255)
                                                                                      gATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGaaa                                                 >  1:1601171/1‑140 (MQ=255)
                                                                                        ttACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGt                                               >  1:3001801/1‑140 (MQ=255)
                                                                                        tgaCCCGCATGAAAGAGTACGGCCAGCTGCGATTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATGGCCCTATCCTATCGAACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGt                                               <  2:5120058/138‑1 (MQ=255)
                                                                                         tACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTc                                              >  1:3729947/1‑140 (MQ=255)
                                                                                             cGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                                          <  1:8480097/140‑1 (MQ=255)
                                                                                             cGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                                          <  1:7921613/140‑1 (MQ=255)
                                                                                             cGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                                          <  1:3121860/140‑1 (MQ=255)
                                                                                              gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctgc                                         >  1:10013296/1‑140 (MQ=255)
                                                                                               cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGAAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctgct                                        <  2:10243960/140‑1 (MQ=255)
                                                                                                 tGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTAt                                      >  2:3661808/1‑140 (MQ=255)
                                                                                                       agTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCACCTATTTTACCGGTCCGGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTAt                                <  2:2049654/140‑1 (MQ=255)
                                                                                                        gTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                               <  2:932707/140‑1 (MQ=255)
                                                                                                        gTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                               <  1:10282610/140‑1 (MQ=255)
                                                                                                        gTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                               <  1:1899911/140‑1 (MQ=255)
                                                                                                        gTACGGCCAGCTGCGATTTCGCGTTTCACTGGTCAACTAATTTACCGGTCCTGGCAGTATTGCCAGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAATCAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                               <  2:1319662/140‑1 (MQ=255)
                                                                                                        ctccGGCCCGCTGCGTTGACGCTTTTCACTCGGAAACTAATTTACCGGGCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACTATGGTGGTGGGTAAACAGCGGATGCCAGAACGTCGCTGCTATTTTTATc                               <  2:7086669/137‑1 (MQ=255)
                                                                                                         gaCGGCCAGCTGCGTTTTCGCGTGTCACTGGGCAACTATTTTACCGGGCCTGGCAGTATTTCCCGATCCGATCGTACGGCGAATACGACGATGGTGGGGGGTAAACAGCGGATGCAAGAAAGTCGCTGCGTTTTTTATcc                              <  2:8366621/139‑2 (MQ=255)
                                                                                                           cGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAgg                            <  2:444067/140‑1 (MQ=255)
                                                                                                            ggCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGACGAAAACGACTATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGa                           >  2:486418/1‑140 (MQ=255)
                                                                                                            ggCCAGCTGCGTGTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGACAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGa                           >  2:3501704/1‑140 (MQ=255)
                                                                                                             gCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGAt                          <  2:1841275/140‑1 (MQ=255)
                                                                                                             gCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGAt                          <  1:2592411/140‑1 (MQ=255)
                                                                                                             gCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGAt                          <  1:2828430/140‑1 (MQ=255)
                                                                                                             gCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGAt                          <  1:77934/140‑1 (MQ=255)
                                                                                                             gCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGAGGCCAGAAAGTCGCTGCTATTTTTATCAGGAt                          <  2:1808168/140‑1 (MQ=255)
                                                                                                             gCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATAGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGAt                          <  2:9605758/140‑1 (MQ=255)
                                                                                                              ccAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGAt                                                         <  1:7715130/108‑1 (MQ=255)
                                                                                                              ccAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATc                         >  1:8748396/1‑140 (MQ=255)
                                                                                                              ccAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATc                         >  1:545170/1‑140 (MQ=255)
                                                                                                              ccAGCTGCGTTTTCGCGTTTCACTGGGCAAATATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATc                         >  2:1729677/1‑140 (MQ=255)
                                                                                                               cAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCt                        <  2:8748395/140‑1 (MQ=255)
                                                                                                                  cTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAt                     <  1:3554193/140‑1 (MQ=255)
                                                                                                                    gCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGt                   <  1:3617108/140‑1 (MQ=255)
                                                                                                                    gCGTTTGCGCGTTTCACTGGGCAACTATTTTACCGGTACTGGCAGTCTGGCCCGATCCTATCGTACGGCGAAAACGACGTTGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGt                   <  2:7241256/140‑1 (MQ=255)
                                                                                                                       ttttCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGttcc                >  2:974521/1‑138 (MQ=255)
                                                                                                                       ttttCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTAc                <  2:218831/140‑1 (MQ=255)
                                                                                                                          tCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCtg             <  2:6486257/140‑1 (MQ=255)
                                                                                                                            gcgTGTCACTGGGCAACTATTTTACCGGGCCTGGCCGTATTGCCCGATCCTATAGTACGGCGATAACGACGATGTTGGGGGGTAAACAGCGGATGCCAGAAAGGCGCTGCTATGTTTAGCAGGATCTGATGTTAAGtgtg           >  2:3242788/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CTAACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTGTGTTACTCGAC  >  NZ_CP025268/182909‑183181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑