BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,415,086	G→C	T101S (ACC→AGC)	yfbS ←	SLC13 family permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,415,086	0	G	C	99.6%	1654.0 / NA	477	T101S (ACC→AGC)	yfbS	SLC13 family permease
Reads supporting (aligned to +/- strand): ref base G (0/1); major base C (204/270); minor base A (1/1); total (205/272)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 477 total aligned reads displayed.

AATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414950‑2415222

aaTCAGCCCGGCAAAACTTAACGGCATCATCATACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACAACACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCa                                                                                                                                       >  2:903216/1‑140 (MQ=255)

aaTCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCa                                                                                                                                       >  1:8139663/1‑140 (MQ=255)

  tCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGc                                                                                                                                     <  2:273981/140‑1 (MQ=255)

  tCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGc                                                                                                                                     <  1:465505/140‑1 (MQ=255)

   cAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCa                                                                                                                                    <  1:6514368/140‑1 (MQ=255)

      cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  2:8619293/1‑140 (MQ=255)

        cGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATcaac                                                                                                                               >  1:6143621/1‑140 (MQ=255)

        cGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATcaac                                                                                                                               <  2:8327107/140‑1 (MQ=255)

          gCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATcaacaa                                                                                                                             <  2:4355441/140‑1 (MQ=255)

          gCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATcaacaa                                                                                                                             <  1:2618344/140‑1 (MQ=255)

           cAAAACTTAACGGNATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCANGCCCCGCGACGCTCAGCATCAACaaa                                                                                                                            >  1:5752973/1‑140 (MQ=255)

                    aCGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAtt                                                                                                                   <  2:5223343/140‑1 (MQ=255)

                      ggCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAtttt                                                                                                                 <  1:10014984/140‑1 (MQ=255)

                      ggCATCATCAGACGTGACGGCGACGTTAGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGCTAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAtttt                                                                                                                 <  2:10155840/140‑1 (MQ=255)

                        catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGa                                                                                                               >  2:6837431/1‑140 (MQ=255)

                                  cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  1:9344015/1‑140 (MQ=255)

                                  cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  2:6318717/1‑140 (MQ=255)

                                    tGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGc                                                                                                   >  1:739540/1‑140 (MQ=255)

                                     gACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGcc                                                                                                  >  2:3173420/1‑140 (MQ=255)

                                      aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 >  2:4510228/1‑140 (MQ=255)

                                       cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  1:8597175/140‑1 (MQ=255)

                                       cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  1:1016103/140‑1 (MQ=255)

                                       cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  1:7811573/140‑1 (MQ=255)

                                          cGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCaa                                                                                             >  1:6723154/1‑140 (MQ=255)

                                           gACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAAc                                                                                            >  2:6696694/1‑140 (MQ=255)

                                                  gCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACACCGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAcc                                                                                     >  1:1809129/1‑140 (MQ=255)

                                                      aCGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGcc                                                                                 <  2:10488843/140‑1 (MQ=255)

                                                        gCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGTTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCAt                                                                               <  2:2020636/140‑1 (MQ=255)

                                                        gCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCAt                                                                               <  2:6186217/140‑1 (MQ=255)

                                                          aTGGCAACGCTTAACACCACTGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCACCGCGACGCACAGCATCAACAAAACCACCATTTTGATTTCACTCTTGCCCGCAACTTTGTCCAGCCCTGt                                                                             <  2:8237105/140‑1 (MQ=255)

                                                          aTGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGt                                                                             <  2:9322487/140‑1 (MQ=255)

                                                          aTGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGt                                                                             <  1:8000054/140‑1 (MQ=255)

                                                            ggCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTc                                                                           >  1:10625818/1‑140 (MQ=255)

                                                             gCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTcc                                                                          >  1:9822043/1‑140 (MQ=255)

                                                             gCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTcc                                                                          >  2:7780830/1‑140 (MQ=255)

                                                             gCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTcc                                                                          >  2:2559414/1‑140 (MQ=255)

                                                               aaCGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCa                                                                        <  1:4755445/140‑1 (MQ=255)

                                                                 cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                      <  2:7468027/140‑1 (MQ=255)

                                                                  gCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTa                                                                     >  1:8364532/1‑140 (MQ=255)

                                                                   cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                    <  2:9368336/140‑1 (MQ=255)

                                                                   cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                    <  2:5360865/140‑1 (MQ=255)

                                                                      aaCACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCACATTTGACCAGCCATGTTCCCATTACGGt                                                                 >  2:4041144/1‑140 (MQ=255)

                                                                      aaCACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGt                                                                 >  1:3658228/1‑140 (MQ=255)

                                                                        caccacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTgg                                                               <  1:3882078/140‑1 (MQ=255)

                                                                         accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:9696988/140‑1 (MQ=255)

                                                                            acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  1:6988996/1‑140 (MQ=255)

                                                                             cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:321519/1‑140 (MQ=255)

                                                                             cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:2050852/1‑140 (MQ=255)

                                                                             cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:8987686/1‑140 (MQ=255)

                                                                             cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  2:7339837/1‑140 (MQ=255)

                                                                             cGGGAATAAAGATAGCGACAACGCCGGATGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAACACCAACATTTTGATTTCACTAGTGCCCGCAACTTTGACCAGCCAGGGTCCCATTACGGTGGCAaca                                                          <  1:5611624/140‑1 (MQ=255)

                                                                                  aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     >  2:3457528/1‑140 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCATCAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  2:5746485/140‑1 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  1:9668441/140‑1 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  1:5144467/140‑1 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  1:6301556/140‑1 (MQ=255)

                                                                                        aTAGCGACAACGCCGTTTGAACTCATAAACGCGCCAAGACCAGCGACGATCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGaa                                               >  2:1503483/1‑139 (MQ=255)

                                                                                          aGCGACAACGCCGGTTGAACTCCTACACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATGTTGATTTCACTCTTGCCCGCAACGTTGACCAGACAGGTTCCGATTACGGTGGCACCACCGGTACGGTccg                                             >  2:7108859/1‑139 (MQ=255)

                                                                                          aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                    <  2:5606319/117‑1 (MQ=255)

                                                                                          aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             >  1:9983669/1‑140 (MQ=255)

                                                                                          aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             >  1:8721584/1‑140 (MQ=255)

                                                                                          aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             >  1:5923646/1‑140 (MQ=255)

                                                                                           gCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaa                                            >  1:10425537/1‑140 (MQ=255)

                                                                                           gCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaa                                            >  2:10348613/1‑140 (MQ=255)

                                                                                            cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                           <  1:1839190/140‑1 (MQ=255)

                                                                                            cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                           >  2:10315061/1‑140 (MQ=255)

                                                                                              aCAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAAcc                                         <  1:4020698/140‑1 (MQ=255)

                                                                                                   gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:9506388/140‑1 (MQ=255)

                                                                                                   gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  1:8227624/140‑1 (MQ=255)

                                                                                                   gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:4744102/140‑1 (MQ=255)

                                                                                                   gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  1:2889679/140‑1 (MQ=255)

                                                                                                    ccGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGcc                                   >  2:2397301/1‑140 (MQ=255)

                                                                                                    ccGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGcc                                   <  1:3294114/140‑1 (MQ=255)

                                                                                                       gTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaat                                <  1:8926004/140‑1 (MQ=255)

                                                                                                            aCTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAAtaataa                           >  2:980374/1‑140 (MQ=255)

                                                                                                              tCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGcc                                   <  1:4243542/130‑1 (MQ=255)

                                                                                                                        gcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATGTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCACACCATCGCCAATAATAAACAAGGCGGCaa               >  1:9180693/1‑140 (MQ=255)

                                                                                                                         ccACGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTCTTGCCCGCAACTTTGACCAGCCCAGTTCCCATGACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATCAACAAGGCGGCAAt              <  2:10681328/140‑1 (MQ=255)

                                                                                                                         ccAAGCCCCGCGACGCTCAGCATCAACAAACCCAACCTCTTGATTTCACTAGTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAAGAAACAAGGCGGCAAt              <  2:321699/140‑1 (MQ=255)

                                                                                                                         ccAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTTATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAAt              <  1:3383929/140‑1 (MQ=255)

                                                                                                                         ccAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAAt              <  1:7665460/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCGGGa         <  1:4521160/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:6529072/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:4423648/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:5401540/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:2793664/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:1188785/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:8068575/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:2312991/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:3737200/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:4983641/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:1643614/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:607573/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAACAAAACCAACATTTAGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:1859011/140‑1 (MQ=255)

                                                                                                                               cccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGAc        <  1:1217708/140‑1 (MQ=255)

                                                                                                                                 cgcgACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACaa      >  1:5317480/1‑140 (MQ=255)

                                                                                                                                  gcgACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     >  1:10519019/1‑92 (MQ=255)

                                                                                                                                   cgACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACg    <  2:3013338/140‑1 (MQ=255)

                                                                                                                                   cgACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACg    <  1:10275139/140‑1 (MQ=255)

                                                                                                                                     aCGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGtt  <  1:3466295/140‑1 (MQ=255)

AATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414950‑2415222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`