BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,529,956	T→C	309 (TAA→TAG)	yfeR ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,529,956	0	T	C	100.0%	1723.1 / NA	494	309 (TAA→TAG)	yfeR	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (237/257); total (237/257)

Only 100 of 494 total aligned reads displayed.

CACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCA‑‑‑‑‑‑‑‑‑CACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAA  >  NZ_CP025268/2529828‑2530092

cacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacatctgattgattactgccggggctcccggcagtatgaattCACTCta                                                                                                                                        >  1:3152435‑M2/134‑140 (MQ=255)

 acattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtat                                                                                                                                       <  1:694721‑M2/8‑1 (MQ=255)

  cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                      <  1:10834842‑M2/9‑1 (MQ=255)

  cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                      >  1:5324783‑M2/132‑140 (MQ=255)

   attactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtctgaattCACTCTatat                                                                                                                                     >  2:3039773‑M2/131‑140 (MQ=255)

   attactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTatat                                                                                                                                     >  2:307059‑M2/131‑140 (MQ=255)

     tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCt                                                                                                                                   >  2:3181508‑M2/129‑140 (MQ=255)

      actacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTg                                                                                                                                  >  1:9167208‑M2/128‑140 (MQ=255)

         acggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATa                                                                                                                               <  2:1710017‑M2/16‑1 (MQ=255)

          cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                              <  1:6206516‑M2/17‑1 (MQ=255)

          cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                              >  2:6092548‑M2/124‑140 (MQ=255)

            gggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACaa                                                                                                                            <  2:4862039‑M2/19‑1 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                           >  2:589324‑M2/121‑140 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                           >  1:7593837‑M2/121‑140 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                           <  1:9599826‑M2/20‑1 (MQ=255)

              gattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACg                                                                                                                          <  1:4992527‑M2/21‑1 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                                 <  2:1471140‑M2/14‑1 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  1:8910237‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  1:8590296‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  2:3663693‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  1:6268102‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACg                                                                                                                          >  2:8827093‑M2/119‑139 (MQ=255)

                   agtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATcc                                                                                                                     <  1:9713589‑M2/26‑1 (MQ=255)

                   agtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATcc                                                                                                                     <  1:10214821‑M2/26‑1 (MQ=255)

                        cgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTc                                                                                                                <  1:13117‑M2/31‑1 (MQ=255)

                            atgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGc                                                                                                            <  1:4158684‑M2/35‑1 (MQ=255)

                             tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                           <  2:6790489‑M2/36‑1 (MQ=255)

                             tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                           <  1:7522024‑M2/36‑1 (MQ=255)

                              gcggatgacaaactgggcgaagattattaccaggatgcgataaactactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                          <  1:8709400‑M2/37‑1 (MQ=255)

                               cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                         <  2:2277996‑M2/38‑1 (MQ=255)

                                 gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                                       >  1:5551277‑M2/101‑140 (MQ=255)

                                 gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                                       <  2:4146307‑M2/40‑1 (MQ=255)

                                    gacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                      <  2:7741712‑M2/9‑1 (MQ=255)

                                      caaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa                                                                                                  <  2:8590295‑M2/45‑1 (MQ=255)

                                      caaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa                                                                                                  <  2:2891186‑M2/45‑1 (MQ=255)

                                           tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATCTCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  1:566753‑M2/50‑1 (MQ=255)

                                           tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  1:5073266‑M2/50‑1 (MQ=255)

                                           tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  1:4459181‑M2/50‑1 (MQ=255)

                                           tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  1:8164300‑M2/50‑1 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATc                                                                                                                                    >  2:9237691‑M2/90‑100 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                              >  2:5626831‑M2/90‑106 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcgc                                                                                              >  1:10466136‑M2/90‑138 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                            >  1:104911‑M2/90‑140 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                            <  2:1944091‑M2/51‑1 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                            <  1:4749194‑M2/51‑1 (MQ=255)

                                            gggCGTAGATTTTTGCCCGGCTGCGTTACTCTACTCCAGCCAATTTAAGTTGTGATTGATTACTGCCGGGGCGCCCGGCAGTATGACTTCACTCTCTATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcgctt                                                                                            >  2:4741374/1‑138 (MQ=255)

                                               cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGc                                                                                         >  1:9782229‑M2/87‑140 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                       <  1:3399636‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                       <  1:5955741‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                       <  2:5103222‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                       <  1:804789‑M2/56‑1 (MQ=255)

                                                   gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                      >  2:8139634‑M2/83‑91 (MQ=255)

                                                   gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                     <  2:3807220‑M2/58‑1 (MQ=255)

                                                      ttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc                                                                                  <  1:5857824‑M2/61‑1 (MQ=255)

                                                       tttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                           <  1:671221‑M2/36‑1 (MQ=255)

                                                       tttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtct                                                                                 >  1:3304297‑M2/79‑140 (MQ=255)

                                                       tttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtct                                                                                 >  1:6453275‑M2/79‑140 (MQ=255)

                                                             aggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                           <  1:10711508‑M2/68‑1 (MQ=255)

                                                             aggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                           <  1:2387545‑M2/68‑1 (MQ=255)

                                                              ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                                          >  2:6284317‑M2/72‑140 (MQ=255)

                                                                atgcgataatctactccatcaaatttaacttctgattgattgctgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                        <  2:8380675‑M2/71‑1 (MQ=255)

                                                                atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                        <  2:10240964‑M2/71‑1 (MQ=255)

                                                                         tctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                               >  1:9348862‑M2/61‑140 (MQ=255)

                                                                         tctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                               >  2:348210‑M2/61‑140 (MQ=255)

                                                                             ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                           >  1:4815031‑M2/57‑140 (MQ=255)

                                                                             ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                           >  1:8474036‑M2/57‑140 (MQ=255)

                                                                             ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                           >  1:2225105‑M2/57‑140 (MQ=255)

                                                                              tccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTc                                                          >  1:4366563‑M2/56‑140 (MQ=255)

                                                                              tccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTc                                                          >  2:9796974‑M2/56‑140 (MQ=255)

                                                                                catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                        >  1:7051965‑M2/54‑140 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtctgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                      >  2:9085481‑M2/52‑140 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCTCTCTATATCTGATACAACGGAGCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCAGAAAGCTTCAgcg                                                      >  2:8676235‑M2/52‑140 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                                 <  1:264056‑M2/14‑1 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                      >  2:10622902‑M2/52‑140 (MQ=255)

                                                                                       tttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTGCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                                 <  1:9999519‑M2/94‑1 (MQ=255)

                                                                                          aacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  2:4544570‑M2/50‑1 (MQ=255)

                                                                                          aacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             >  1:4544570‑M2/44‑93 (MQ=255)

                                                                                          aacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAACACGTCCCATAAAGCTTCAGCGGCGGTGGa                                              <  2:6537504‑M2/97‑1 (MQ=255)

                                                                                               ctgattgattactgccggggctcccggcagtatgaattCACTCTCTATCTGATACAACGGATCCCCTTCCCGCCCAGCCCTTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                                         >  2:716361‑M2/39‑140 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                        <  1:8364181‑M2/103‑1 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                        <  1:9232495‑M2/103‑1 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACCCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                        <  2:9681491‑M2/103‑1 (MQ=255)

                                                                                                  attgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                                      <  2:1222551‑M2/105‑1 (MQ=255)

                                                                                                  attgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                                      <  2:7439900‑M2/105‑1 (MQ=255)

                                                                                                   ttgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACg                                     <  2:5268560‑M2/106‑1 (MQ=255)

                                                                                                             ccggggctcccggcagtatgacttCACTCTATATCTGATCCAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCCCGTCCCATAAAGCTTCAGCGGCGGGGGACAGTGAACGGTTCTTCCTg                           >  1:10079371‑M2/25‑140 (MQ=255)

                                                                                                             ccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg                           <  2:2424768‑M2/116‑1 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                        >  2:10784407‑M2/22‑140 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                        >  2:9490026‑M2/22‑140 (MQ=255)

                                                                                                                 ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                       <  1:1899462‑M2/120‑1 (MQ=255)

                                                                                                                 ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                       <  1:10611068‑M2/120‑1 (MQ=255)

                                                                                                                     cccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg                   >  1:28209‑M2/17‑140 (MQ=255)

                                                                                                                     cccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg                   >  1:2922832‑M2/17‑140 (MQ=255)

                                                                                                                       cggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCa                 <  1:1621379‑M2/126‑1 (MQ=255)

                                                                                                                           agtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCaa             >  1:3744965‑M2/11‑140 (MQ=255)

                                                                                                                            gtatgaattCACTCTTTATCTGATACAACGGATCCCCTTCCCGCCCATCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTTAACGGTTCTTCCTGCGCACCAGCAtcatc            <  2:2707064‑M2/131‑3 (MQ=255)

                                                                                                                            gtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTACCTGCGCACCAGCATCAAc            <  2:4355562‑M2/131‑1 (MQ=255)

                                                                                                                               tgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                         <  2:10387863‑M2/38‑1 (MQ=255)

                                                                                                                                  attCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGt      >  2:3133091‑M2/4‑140 (MQ=255)

                                                                                                                                  attCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGt      >  2:5432122‑M2/4‑140 (MQ=255)

CACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCA‑‑‑‑‑‑‑‑‑CACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAA  >  NZ_CP025268/2529828‑2530092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`