BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	306,029	C→T	V786M (GTG→ATG)	ecpC ←	fimbrial usher EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	306,029	0	C	T	99.0%	1925.6 / NA	507	V786M (GTG→ATG)	ecpC	fimbrial usher EcpC
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (259/242); minor base G (2/3); total (261/246)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 510 total aligned reads displayed.

CGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCA  >  NZ_CP025268/305892‑306165

cgccgcCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGt                                                                                                                                        >  1:7835288/1‑140 (MQ=255)

cgccgcCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGt                                                                                                                                        >  1:9047115/1‑140 (MQ=255)

  ccgccCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTcc                                                                                                                                      >  1:6120283/1‑140 (MQ=255)

        aCGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACa                                                                                                                                <  2:9336531/140‑1 (MQ=255)

          gATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACaaa                                                                                                                              <  1:5610732/140‑1 (MQ=255)

                    cGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGttt                                                                                                                    >  1:1198483/1‑140 (MQ=255)

                      cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTc                                                                                                                  >  1:7641068/1‑140 (MQ=255)

                      cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTc                                                                                                                  >  1:4791166/1‑140 (MQ=255)

                        gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                <  2:4122146/140‑1 (MQ=255)

                             ccACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggtt                                                                                                           <  1:6272114/140‑1 (MQ=255)

                                 aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                       >  1:186175/1‑140 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     >  1:873962/1‑140 (MQ=255)

                                     ccGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggttcg                                                                                                   <  1:9394603/140‑1 (MQ=255)

                                      cGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGgttcgggttcgg                                                                                                  >  1:1122975/1‑140 (MQ=255)

                                       gACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                 <  2:223223/140‑1 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  2:8867724/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  1:6671761/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  2:5224856/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             >  1:6502418/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             >  2:9164958/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             >  1:4149404/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGAGAGTGGGGTATTTCTTCTCCATGTCCATGACAAACTCGCCGTGTTCATCGGTTCGGGTGCGGCCGat                                                                                             >  2:7135968/1‑140 (MQ=255)

                                            aGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGata                                                                                            <  1:3128484/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           >  2:1801692/1‑140 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:68338/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:37275/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:397860/140‑1 (MQ=255)

                                                  cACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCCCTGTAGCGAAAATCGATAGTGGGGTATGTCTTAGCCATGTCCATGACAATCTCGCGGTTTTCAGCGGTGCGGGTTCGGCCGATGTGggtg                                                                                      >  2:10651878/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  2:10154273/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  2:5540815/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  1:10454332/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  1:5048161/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     <  1:2271907/140‑1 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  1:5853029/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  1:4366459/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:7157471/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGGTCGGCCGATATGGTTGTTAAt                                                                                 >  1:7561325/1‑140 (MQ=255)

                                                         cgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc                                                                               <  2:10309774/140‑1 (MQ=255)

                                                            cTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTg                                                                            >  1:8879570/1‑140 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  1:983688/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:3406217/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:5048161/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:4406379/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  1:3598406/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATAGGGTTGTTAATCCGTGCg                                                                          <  1:4471667/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACNCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  1:10049795/140‑1 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  >  1:2909779/1‑140 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  <  1:15263/140‑1 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  <  2:9522068/140‑1 (MQ=255)

                                                                        cAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagca                                                                >  1:7285311/1‑140 (MQ=255)

                                                                         agagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagcag                                                               >  1:10593629/1‑140 (MQ=255)

                                                                         agagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagcag                                                               >  1:6428969/1‑140 (MQ=255)

                                                                            gCCACTTCGCAGGTATTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAgtgt                                                            <  2:5437447/140‑1 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCTTGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         <  2:5686110/140‑1 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         <  2:9663969/140‑1 (MQ=255)

                                                                                cTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCg                                                        >  2:8024271/1‑140 (MQ=255)

                                                                                 ttCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGt                                                       >  2:7854193/1‑140 (MQ=255)

                                                                                    gCAGGTTTTATTGCCACTGGAGCGAAAATCGAGAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCtt                                                    <  2:6011041/140‑1 (MQ=255)

                                                                                       ggTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCg                                                 >  1:8481114/1‑140 (MQ=255)

                                                                                        gctttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                >  2:6555032/3‑140 (MQ=255)

                                                                                        gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  2:873962/140‑1 (MQ=255)

                                                                                        gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  2:5004155/140‑1 (MQ=255)

                                                                                        gTTTTATTGCCACCGTAGCGAAAATCGAGAGGGGGGTATTTCTTCTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  2:7777050/140‑1 (MQ=255)

                                                                                         ttttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCa                                               <  2:1188948/140‑1 (MQ=255)

                                                                                          tttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCAc                                              >  1:10169275/1‑140 (MQ=255)

                                                                                             aTTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGa                                           <  2:7889669/140‑1 (MQ=255)

                                                                                             aTTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGa                                           >  2:4602317/1‑140 (MQ=255)

                                                                                              ttGCCTCTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGAt                                          <  1:9190913/140‑1 (MQ=255)

                                                                                              ttGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGAt                                          <  2:2930602/140‑1 (MQ=255)

                                                                                               tGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATa                                         <  2:1503068/140‑1 (MQ=255)

                                                                                                  cACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGa                                      >  1:8596374/1‑140 (MQ=255)

                                                                                                   aCTGTAGCGATAATCGAGAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTCGCCAGCAGTGTGCCGGCGTCCGCACGGATACGAc                                     >  2:3622155/1‑140 (MQ=255)

                                                                                                   aCTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:3779182/86‑1 (MQ=255)

                                                                                                    cTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGAcc                                    >  1:1365195/1‑140 (MQ=255)

                                                                                                          cGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           >  2:8526960/1‑79 (MQ=255)

                                                                                                            aaaaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACgg                            >  2:1621149/1‑140 (MQ=255)

                                                                                                            aaaaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACgg                            >  1:5726927/1‑140 (MQ=255)

                                                                                                            aaaaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACgg                            >  1:3440788/1‑140 (MQ=255)

                                                                                                            aaaaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACgg                            >  1:337063/1‑140 (MQ=255)

                                                                                                             aaaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGt                           <  2:3015655/140‑1 (MQ=255)

                                                                                                              actCGATAGTGGGGTATTTCTTCTCCAGGTCCATGACAAACTCGCCGTTGTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTa                          <  1:8144099/138‑1 (MQ=255)

                                                                                                              aaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTa                          <  2:6986169/140‑1 (MQ=255)

                                                                                                               aTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGGTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGCGACGGTaa                         >  2:2655423/1‑140 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           >  2:8228395/1‑70 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCAt                     <  2:3776578/140‑1 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCAt                     >  2:7483877/1‑140 (MQ=255)

                                                                                                                    tAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATTGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATc                    <  2:5884245/140‑1 (MQ=255)

                                                                                                                    tAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATc                    <  1:9634021/140‑1 (MQ=255)

                                                                                                                        ggggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCt                <  2:3245889/140‑1 (MQ=255)

                                                                                                                         gggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCtt               <  2:7949187/140‑1 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTACACCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct          <  1:5296034/140‑1 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct          <  1:2470729/140‑1 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTGAGCCAGCAGGGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTCACCCTCTGCTTCTCct          >  2:10508551/1‑140 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTAGTTAATCCGTGCGTTAGCCAGCAGCGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct          <  2:2451018/140‑1 (MQ=255)

                                                                                                                                   tATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGTCCGGAGACGGTAACCATCTGCTTCACCTCTGGc     >  2:8354511/1‑140 (MQ=255)

                                                                                                                                   tATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGc     >  1:2180873/1‑140 (MQ=255)

                                                                                                                                   tATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTGCACCTCTGGc     >  2:141104/1‑140 (MQ=255)

                                                                                                                                    aTCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggttc                                                                                                    >  1:10752329/1‑44 (MQ=255)

                                                                                                                                    aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCca                                                                   >  1:8033173/1‑77 (MQ=255)

                                                                                                                                     tccatgtccatgACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTc   >  1:6850511/1‑140 (MQ=255)

CGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCA  >  NZ_CP025268/305892‑306165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`