BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,913,061	T→C	112 (TAA→TAG)	mazF ←	endoribonuclease MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,913,061	0	T	C	99.9%	2316.7 / NA	663	112 (TAA→TAG)	mazF	endoribonuclease MazF
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (399/263); minor base G (1/0); total (400/263)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 666 total aligned reads displayed.

ACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCT  >  NZ_CP025268/2912924‑2913197

    gCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCa                                                                                                                                    <  1:7273000/140‑1 (MQ=255)

        ccGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCa                                                                                                                                >  2:7285118/1‑140 (MQ=255)

                  gATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAAt                                                                                                                      >  1:9625080/1‑140 (MQ=255)

                    tCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAAttt                                                                                                                    <  2:5344768/140‑1 (MQ=255)

                                 gCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTGAATTTTGGCTTTAATGAg                                                                                                       >  1:9259627/1‑140 (MQ=255)

                                  cATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      >  2:4164553/1‑140 (MQ=255)

                                  cATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      <  2:2540391/140‑1 (MQ=255)

                                   ataataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGtt                                                                                                     <  1:9391536/140‑1 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATATAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  2:8876337/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      <  2:63166/132‑1 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  1:1028783/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  2:3647755/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  1:2862272/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  1:3732775/1‑140 (MQ=255)

                                           gAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTcc                                                                                             <  2:1020830/140‑1 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAcc                                                                                                                                      <  2:1815440/98‑1 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTaa                                                                                                 <  1:2389190/135‑1 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAAttaat                                                                                            >  1:8635120/1‑137 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:8278913/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:5626083/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:3907864/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:4744244/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:3364141/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:2094342/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:2636906/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGACAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:9841072/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATAGCGGGTCTGTCAGGTGGAAACCTGTGACCCGAATAGAAGTGAGTTAGTAACACTACCCAATCCGTCCGTTCATTTTGGCTTTAATGCGTTGTAATTcgt                                                                                            >  2:10623483/1‑138 (MQ=255)

                                                aaCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCCGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTgg                                                                                        <  2:5738227/140‑1 (MQ=255)

                                                aaCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTgg                                                                                        <  2:8714107/140‑1 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAAttt                                                                                                                    >  2:3162756/1‑111 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                            <  1:5170116/119‑1 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                            >  2:5170116/1‑119 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTaa                                                                                                 <  1:9833521/130‑1 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTggg                                                                                       >  1:598590/1‑140 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTggg                                                                                       >  2:7127160/1‑140 (MQ=255)

                                                  cGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTACTCTgggg                                                                                      >  1:3254100/1‑140 (MQ=255)

                                                    gTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCa                                                                                    >  1:10858571/1‑140 (MQ=255)

                                                     tCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                                                   <  1:10547299/140‑1 (MQ=255)

                                                      cGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATc                                                                                                                                 >  2:2248879/1‑93 (MQ=255)

                                                      cGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAAc                                                                                  >  2:9469876/1‑140 (MQ=255)

                                                        aTTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTg                                                                                <  1:6066061/140‑1 (MQ=255)

                                                         tttGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGt                                                                               <  2:3907864/140‑1 (MQ=255)

                                                               tCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCttt                                                                         >  1:4394963/1‑140 (MQ=255)

                                                               tCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCttt                                                                         <  1:1691418/140‑1 (MQ=255)

                                                                  ttGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttctt                                                                      <  1:8397483/140‑1 (MQ=255)

                                                                   tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACg                                                                                                                           >  2:48396/1‑86 (MQ=255)

                                                                   tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttcttc                                                                     >  2:433131/1‑140 (MQ=255)

                                                                   tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttcttc                                                                     >  1:1293734/1‑140 (MQ=255)

                                                                   tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttcttc                                                                     >  1:3396077/1‑140 (MQ=255)

                                                                       ttGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGc                                                                                     <  2:963631/120‑1 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttctt                                                                      <  1:2742759/132‑1 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctc                                                              >  1:1840996/1‑140 (MQ=255)

                                                                            cTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  2:2721380/95‑1 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAAt                                                                                                          >  1:7506123/1‑86 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      <  1:9977414/90‑1 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGtt                                                                              <  1:1392677/114‑1 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  2:5821263/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  2:8068077/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  1:5727322/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  2:1299439/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    <  1:4869452/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  2:8472957/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  1:21319/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  1:6856481/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  2:7830842/140‑1 (MQ=255)

                                                                                                 ggTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACtt                                       <  2:598590/140‑1 (MQ=255)

                                                                                                    ggAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACttttt                                    <  2:2890111/140‑1 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      >  2:9057178/1‑70 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctcc                                                             <  2:6827979/111‑1 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCt                                >  1:8936008/1‑140 (MQ=255)

                                                                                                          cTGTGACCAGAATATAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:1713772/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACTTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  1:6616133/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTNTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  1:10049585/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  1:4615031/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:1607798/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:8552954/140‑1 (MQ=255)

                                                                                                           tgtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTNTACCTGAt                             <  1:5821263/140‑1 (MQ=255)

                                                                                                            gtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTACTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATc                            <  1:8110179/140‑1 (MQ=255)

                                                                                                             tgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCt                                                                                                               >  1:6249728/1‑56 (MQ=255)

                                                                                                             tgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTgg                                                                                        >  1:3520449/1‑79 (MQ=255)

                                                                                                             tgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  1:9195089/123‑1 (MQ=255)

                                                                                                               aCCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGc                         <  1:3053324/140‑1 (MQ=255)

                                                                                                                  aGAATAGAAGTGAGTTATTAACACTACCCAATCAGTACGTTAATTTTGTCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTaa                      <  2:6607269/140‑1 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACg                    <  2:6180782/140‑1 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACg                    >  2:10388594/1‑140 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTCGCCCGCCAGGCGa                                            >  1:4153091/1‑116 (MQ=255)

                                                                                                                     aTAGAAGTGAGTTAGTAACACTACCCAATCAGGACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCGTCGTTGCTCCTCTTGCCCGCCAGGCGATACTGTTTACCTGATCAGCTCACGc                   >  2:7004339/1‑140 (MQ=255)

                                                                                                                      tAGAAGTGAGTTAGTCACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCt                  <  2:9214311/140‑1 (MQ=255)

                                                                                                                        gAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTAc                <  1:4744244/140‑1 (MQ=255)

                                                                                                                         aaGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACg               >  1:773993/1‑140 (MQ=255)

                                                                                                                         aaGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACg               <  2:459058/140‑1 (MQ=255)

                                                                                                                         aaGTGAGTTAGTAACACTACCCAATCAGTAAGTTAATTTTGGCTTTAATTAGTTGTAATTCCTCTGGGGCAAATGTTCCTTTCTTCGTTGCTCCTCTTGCACGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACg               >  2:244514/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  1:6387198/45‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCctc                                                                                           >  2:7441748/1‑59 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                                                   <  1:7876732/67‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAgg                                               <  1:4301072/103‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:10864144/120‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          <  1:8549213/140‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:5466454/1‑140 (MQ=255)

                                                                                                                                ttAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCAc        >  2:7586126/1‑140 (MQ=255)

ACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCT  >  NZ_CP025268/2912924‑2913197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`