Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,985,593 T→C *279* (TAA→TAG)  kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,985,5930TC99.8% 2148.8 / NA 616*279* (TAA→TAG) kduI5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (290/324);  minor base A (1/0);  total (292/324)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 616 total aligned reads displayed.

ACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCT  >  NZ_CP025268/2985459‑2985729
                                                                                                                                      |                                                                                                                                        
acaGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcg                                                                                                                                     >  2:6962620/1‑140 (MQ=255)
acaGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcg                                                                                                                                     <  1:6072954/140‑1 (MQ=255)
acaGCCCGCTTGCGCCAGCCCCAACGCCATCCACTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcg                                                                                                                                     >  2:5077691/1‑140 (MQ=255)
 caGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcgc                                                                                                                                    <  1:9697175/140‑1 (MQ=255)
   gCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCaa                                                                                                                                  <  1:4201471/140‑1 (MQ=255)
    cccGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCaaa                                                                                                                                 <  2:10643694/140‑1 (MQ=255)
      cGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATc                                                                                                                               >  2:450140/1‑140 (MQ=255)
        cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                             >  1:9855838/1‑140 (MQ=255)
        cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                             >  1:1739031/1‑140 (MQ=255)
        cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                             >  2:2660598/1‑140 (MQ=255)
          tGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTa                                                                                                                           >  1:7036768/1‑140 (MQ=255)
             gcCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACg                                                                                                                        <  1:8504387/140‑1 (MQ=255)
              ccAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACgg                                                                                                                       <  1:4998599/140‑1 (MQ=255)
                 gCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCca                                                                                                                    <  1:1675612/140‑1 (MQ=255)
                  ccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                   <  1:144794/140‑1 (MQ=255)
                  ccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                   <  2:3809417/140‑1 (MQ=255)
                   cccAACGCCATCCCCTGACCCATTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  2:8282982/140‑1 (MQ=255)
                   cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:10248477/140‑1 (MQ=255)
                   cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  2:4257700/140‑1 (MQ=255)
                   cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:7940392/140‑1 (MQ=255)
                   cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:8682022/140‑1 (MQ=255)
                   cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:3352042/140‑1 (MQ=255)
                   cacAACGACATCCCCTGACCCAGAACATGATGAAAACCAGTGTCGACCGCAACTTTACATTCTAGAGATAATTAACTTTATATAATATCAATACCTTATTCGTTTATGACCACAACTAGCTAAAATATATAACGGCctca                                                                                                                  <  2:7324473/138‑1 (MQ=255)
                       aCGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACATTCGAGAGAAAATGAACTTAAAAGCATAACTATACCTTCTTCGTTTATGACCACCACTCGCGCAATTCTTTAACGGCCGCAgggg                                                                                                              >  2:5690174/1‑139 (MQ=255)
                            aTCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                                                         >  1:1002972/1‑140 (MQ=255)
                            aTCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                                                         >  1:2182543/1‑140 (MQ=255)
                                  tGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATAtcatca                                                                                                   >  1:3564253/1‑140 (MQ=255)
                                       cagtccattATCACAACCATTGACGACCGCAACTTTACCTTCTAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                              <  2:6622379/140‑1 (MQ=255)
                                       cagtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTCGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                              <  2:3383958/140‑1 (MQ=255)
                                          tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  >  1:7517576/1‑117 (MQ=255)
                                          tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTg                                                                                           >  2:8871150/1‑140 (MQ=255)
                                              gtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATTACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTGTAACGGCCACAAGGTCCATATCATCAAAGACCTGGatt                                                                                       >  2:5769208/1‑140 (MQ=255)
                                               tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                              >  2:8524619/1‑132 (MQ=255)
                                                    caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:9235769/107‑1 (MQ=255)
                                                    caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  >  2:9235768/1‑107 (MQ=255)
                                                    caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCa                                                                                                           <  1:111478/114‑1 (MQ=255)
                                                    caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCa                                                                                                           <  2:4652550/114‑1 (MQ=255)
                                                    caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                 >  2:805487/1‑140 (MQ=255)
                                                    caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                 >  1:5533039/1‑140 (MQ=255)
                                                       ccAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACTTGACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                              <  1:6835691/140‑1 (MQ=255)
                                                       ccAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                              <  1:8382790/140‑1 (MQ=255)
                                                         aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTATCGGCCACATGGTCCATATCGTCAAAGACCTGGTTTTCACCGACCAt                                                                            >  2:3265525/1‑140 (MQ=255)
                                                         aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                                                         >  2:10836408/1‑111 (MQ=255)
                                                          gTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATg                                                                           <  1:3193010/140‑1 (MQ=255)
                                                           tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  >  1:5794877/1‑100 (MQ=255)
                                                           tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  2:10391738/100‑1 (MQ=255)
                                                           tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  >  1:8942943/1‑100 (MQ=255)
                                                           tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGc                                                                          >  2:2971490/1‑140 (MQ=255)
                                                           tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGc                                                                          >  1:5369850/1‑140 (MQ=255)
                                                            gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  2:9522010/140‑1 (MQ=255)
                                                            gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  1:10685347/140‑1 (MQ=255)
                                                            gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  1:2000128/140‑1 (MQ=255)
                                                            gacgacCGCAACTTTACCTGCGAGAGAAAATGCACTTAAAATCATCACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  2:2110233/140‑1 (MQ=255)
                                                            gacgacCGCAACTCTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTAGTTTATGCACACACCTAGCGCAAATCATTAACGGCCACATTGTCCATATCAACAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  2:7019837/140‑1 (MQ=255)
                                                                 ccGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                                                         >  1:6690228/1‑103 (MQ=255)
                                                                  cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGag                                                                   >  1:8006872/1‑139 (MQ=255)
                                                                   gCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                  <  1:3781814/140‑1 (MQ=255)
                                                                         ttACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                                            <  2:6201442/140‑1 (MQ=255)
                                                                         ttACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                                            <  1:4885464/140‑1 (MQ=255)
                                                                           aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTaa                                                                                                                          >  1:4408440/1‑76 (MQ=255)
                                                                             cTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAg                                                        <  2:4923226/140‑1 (MQ=255)
                                                                               tCGAGAGAAAATGNACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGNCCATGCCCCAGATAAAGGTATAAGCt                                                      >  1:5748832/1‑140 (MQ=255)
                                                                               tCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACgg                                                                                                                       <  2:1125207/75‑1 (MQ=255)
                                                                               tCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  >  1:1050677/1‑80 (MQ=255)
                                                                               tCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCt                                                      <  2:8752092/140‑1 (MQ=255)
                                                                               tCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCt                                                      <  2:5476555/140‑1 (MQ=255)
                                                                                 gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  >  1:4674223/1‑78 (MQ=255)
                                                                                 gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                  <  1:9421493/126‑1 (MQ=255)
                                                                                  agagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGcc                                                                                                                     >  2:1693239/1‑74 (MQ=255)
                                                                                       aaaTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                              <  2:1964201/140‑1 (MQ=255)
                                                                                           gCACTTAAAATCATAACACTACCTTATTCGGTTATGCCCCCAACTAGCGCACATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  2:361728/140‑1 (MQ=255)
                                                                                           gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  1:6278893/140‑1 (MQ=255)
                                                                                              cTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  2:5881148/106‑1 (MQ=255)
                                                                                               ttAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                   <  1:7783845/63‑1 (MQ=255)
                                                                                                tAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                                     >  2:2240140/1‑140 (MQ=255)
                                                                                                    aTCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGGATAAGCTTTGGTTCCGACACCGGAATgg                                 >  2:396394/1‑140 (MQ=255)
                                                                                                     tCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGa                                <  2:2441250/140‑1 (MQ=255)
                                                                                                     tCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGa                                <  1:7347620/140‑1 (MQ=255)
                                                                                                          aCAATACCTGATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAc                           <  2:9321980/140‑1 (MQ=255)
                                                                                                           cAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAcc                          <  1:4588946/140‑1 (MQ=255)
                                                                                                           cAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAcc                          <  1:8246990/140‑1 (MQ=255)
                                                                                                           cAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAcc                          <  2:640240/140‑1 (MQ=255)
                                                                                                              tACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                              <  1:266627/117‑1 (MQ=255)
                                                                                                              tACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          >  1:5889700/1‑121 (MQ=255)
                                                                                                              tACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCAATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGc                       >  2:7706924/1‑140 (MQ=255)
                                                                                                                 ctcaTTCGTTTATGCCAACAACTAGCGCAAATCTTAAAAGGCCACATGGACCATATCATCAAAGACCTGGTAATCACCGACCATGCCCCAGATAAAGGTATAAGCTATGGATCCGACACCGGAATGGATCGACCAGCTCg                    >  2:1423575/4‑140 (MQ=255)
                                                                                                                  ttATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGt                                                                                                              >  2:7210829/1‑49 (MQ=255)
                                                                                                                  ttATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGt                                                                                                              <  1:7210830/49‑1 (MQ=255)
                                                                                                                  ttATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTg                                                   <  2:6720838/108‑1 (MQ=255)
                                                                                                                   tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                                     <  1:2249833/121‑1 (MQ=255)
                                                                                                                   tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACAGACCATGCCCCAGATAAAGGTAAAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg                  >  2:5289263/1‑140 (MQ=255)
                                                                                                                    aTTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                 <  2:10519601/140‑1 (MQ=255)
                                                                                                                    aTTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                 >  1:2411853/1‑140 (MQ=255)
                                                                                                                     ttCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                        <  2:10045421/52‑1 (MQ=255)
                                                                                                                     ttCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGga                >  1:6460413/1‑140 (MQ=255)
                                                                                                                      tCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                                            >  1:4033578/1‑95 (MQ=255)
                                                                                                                         tttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATc            >  2:7855619/1‑140 (MQ=255)
                                                                                                                          ttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCaa                                                                                                  >  1:2951189/1‑53 (MQ=255)
                                                                                                                          ttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa           >  1:1366340/1‑140 (MQ=255)
                                                                                                                           tATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCAc          >  1:2574131/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                        
ACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCT  >  NZ_CP025268/2985459‑2985729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑