BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,183,692	A→G	S153G (AGT→GGT)	zupT →	zinc transporter ZupT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,183,692	0	A	G	99.8%	1532.6 / NA	439	S153G (AGT→GGT)	zupT	zinc transporter ZupT
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (225/213); minor base C (0/1); total (225/214)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 439 total aligned reads displayed.

GCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTCCCC  >  NZ_CP025268/3183555‑3183828

  acaGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCt                                                                                                                                      <  2:7181980/140‑1 (MQ=255)

      ccATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggca                                                                                                                                  >  1:3718226/1‑140 (MQ=255)

      ccATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggca                                                                                                                                  >  1:9367379/1‑140 (MQ=255)

      ccATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggca                                                                                                                                  >  2:10239031/1‑140 (MQ=255)

         ttCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtg                                                                                                                               >  2:8306272/1‑140 (MQ=255)

          tCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtgg                                                                                                                              <  1:10369064/140‑1 (MQ=255)

          tCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtgg                                                                                                                              <  2:10056167/140‑1 (MQ=255)

              cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                          >  2:5285733/1‑140 (MQ=255)

              cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                          >  2:7222481/1‑140 (MQ=255)

                cACTCTCGGCATCAGTCTGCATAACGTCCCGGAAGGGCTTGCCACCTTTGTCACGGCGAGCAGCACCCTGGAGCTGGGAGTTGGCCTCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCCGTGGCAGGcc                                                                                                                        >  2:8820111/1‑140 (MQ=255)

                 aCTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGccc                                                                                                                       >  1:5342415/1‑140 (MQ=255)

                  ctctCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCg                                                                                                                      <  1:771145/140‑1 (MQ=255)

                   tctcGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCgg                                                                                                                     >  2:5766138/1‑140 (MQ=255)

                    ctcGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGt                                                                                                                    <  2:7694077/140‑1 (MQ=255)

                       ggCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTGGTCACGGCGAGCAGCACCCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTa                                                                                                                 <  2:1564445/140‑1 (MQ=255)

                        gCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTAt                                                                                                                >  2:8220731/1‑140 (MQ=255)

                             aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGc                                                                                                           <  2:9892196/140‑1 (MQ=255)

                             aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGc                                                                                                           >  1:4637911/1‑140 (MQ=255)

                             aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGc                                                                                                           >  1:10188112/1‑140 (MQ=255)

                               tCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCaa                                                                                                         <  1:3853475/140‑1 (MQ=255)

                               tCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCaa                                                                                                         <  1:10082714/140‑1 (MQ=255)

                                cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                        <  2:3809677/140‑1 (MQ=255)

                                cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                        <  1:5832363/140‑1 (MQ=255)

                                cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATACCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                        <  1:10557348/140‑1 (MQ=255)

                                cTGCATAACGTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                        <  2:334069/140‑1 (MQ=255)

                                 tGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACg                                                                                                       <  1:3362486/140‑1 (MQ=255)

                                 agCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACCGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTAGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACg                                                                                                       <  2:5423068/139‑1 (MQ=255)

                                  gCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgg                                                                                                      <  1:1637486/140‑1 (MQ=255)

                                     tAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGt                                                                                                   <  1:8417628/140‑1 (MQ=255)

                                       aCTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCt                                                                                                 <  2:2495167/140‑1 (MQ=255)

                                        cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGACGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                                >  2:894389/1‑140 (MQ=255)

                                        cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                                >  2:9092525/1‑140 (MQ=255)

                                        cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                                >  2:1130673/1‑140 (MQ=255)

                                        cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                                >  1:9634389/1‑140 (MQ=255)

                                         ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTaa                                                                                               >  2:1082366/1‑140 (MQ=255)

                                         ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTaa                                                                                               >  2:4116604/1‑140 (MQ=255)

                                            ccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                            <  1:7464289/140‑1 (MQ=255)

                                             cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGc                                                                                           >  1:6923037/1‑140 (MQ=255)

                                              ggAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGACGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCa                                                                                          <  2:4554575/140‑1 (MQ=255)

                                                         ccACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCtg                                                                               <  1:7957813/140‑1 (MQ=255)

                                                          cACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCTCAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCtgt                                                                              >  2:8498452/1‑140 (MQ=255)

                                                             cTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTggg                                                                           >  1:7083576/1‑140 (MQ=255)

                                                              tttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGc                                                                          >  2:3043266/1‑140 (MQ=255)

                                                               ttGTCACGGCGAGCAGCAACCTGGAGCTGGGCTTTGGCATCGCACTGGACGTCGCGTTGCAAAATATCCGTGAAGGTCTGGCAGGGGCAGGCCAGGTTTCTGCGGCAACGGGGTCTAAACGCACCGCGCTTCTGTGGGCg                                                                         <  2:5698515/140‑1 (MQ=255)

                                                                 gTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                                       <  1:1272393/140‑1 (MQ=255)

                                                                      ggCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGAttt                                                                  >  1:2719140/1‑140 (MQ=255)

                                                                          agcagcAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTgg                                                              >  2:9566389/1‑140 (MQ=255)

                                                                            cagcaACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGAc                                                            >  2:3612817/1‑140 (MQ=255)

                                                                             agcaACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACt                                                           <  1:3239100/140‑1 (MQ=255)

                                                                                 aCCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCa                                                       >  1:5573948/1‑140 (MQ=255)

                                                                                  ccTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAg                                                      <  1:5959974/140‑1 (MQ=255)

                                                                                    tGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                    <  2:10354935/140‑1 (MQ=255)

                                                                                    tGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                    <  1:4094158/140‑1 (MQ=255)

                                                                                    tGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                    >  1:1579786/1‑140 (MQ=255)

                                                                                    tGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                    >  1:309556/1‑140 (MQ=255)

                                                                                    tGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                    <  2:6815976/140‑1 (MQ=255)

                                                                                     ggAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaaa                                                   >  2:1877465/1‑140 (MQ=255)

                                                                                       aGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAAtt                                                 >  2:1822470/1‑140 (MQ=255)

                                                                                       aGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAAtt                                                 <  2:6923036/140‑1 (MQ=255)

                                                                                         cTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCt                                               <  2:6409451/140‑1 (MQ=255)

                                                                                         cTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCt                                               >  1:1135496/1‑140 (MQ=255)

                                                                                           gggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtg                                             >  1:3009833/1‑140 (MQ=255)

                                                                                           gggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtg                                             <  2:9014821/140‑1 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                            >  1:1834454/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                            >  2:2324686/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                            >  1:5683324/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                            >  2:491588/1‑140 (MQ=255)

                                                                                              aTTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCTGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTATGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                          <  1:4343367/140‑1 (MQ=255)

                                                                                              aTTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                          <  1:484640/140‑1 (MQ=255)

                                                                                              aTTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                          <  1:6416212/140‑1 (MQ=255)

                                                                                                 tGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGgtggtg                                       >  1:1430820/1‑140 (MQ=255)

                                                                                                   gCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCATCGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGtgtg                                     <  1:9032586/140‑1 (MQ=255)

                                                                                                   gCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGtgtg                                     <  2:7987750/140‑1 (MQ=255)

                                                                                                   gCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGtgtg                                     <  1:2465081/140‑1 (MQ=255)

                                                                                                   gCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGtgtg                                     <  2:3189984/140‑1 (MQ=255)

                                                                                                      tCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                        <  1:8878477/70‑1 (MQ=255)

                                                                                                      tCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGAGTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTg                                  <  2:3760114/140‑1 (MQ=255)

                                                                                                       cGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                            <  1:7203745/129‑1 (MQ=255)

                                                                                                        gCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                                <  1:6869966/140‑1 (MQ=255)

                                                                                                        gCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                                <  1:8692842/140‑1 (MQ=255)

                                                                                                        gCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                                <  1:9207348/140‑1 (MQ=255)

                                                                                                        gCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                                <  2:2600906/140‑1 (MQ=255)

                                                                                                             ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                           <  1:1072301/140‑1 (MQ=255)

                                                                                                             ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                           <  2:1348845/140‑1 (MQ=255)

                                                                                                              gCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGtt                          >  2:3876823/1‑140 (MQ=255)

                                                                                                                  tCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATc                      <  2:602528/140‑1 (MQ=255)

                                                                                                                  tCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATc                      >  2:6673324/1‑140 (MQ=255)

                                                                                                                  tCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATc                      <  2:4736374/140‑1 (MQ=255)

                                                                                                                    gcgTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCt                    <  1:6184959/140‑1 (MQ=255)

                                                                                                                        tGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGt                >  2:4237060/1‑140 (MQ=255)

                                                                                                                          cacaATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAg              <  1:1944374/140‑1 (MQ=255)

                                                                                                                             aaTATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCAt           <  1:5237541/140‑1 (MQ=255)

                                                                                                                                  cccTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCt                                               <  1:5022142/99‑1 (MQ=255)

                                                                                                                                  cccTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGAttt      >  2:4459781/1‑140 (MQ=255)

                                                                                                                                    cTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                                       <  2:9165382/73‑1 (MQ=255)

                                                                                                                                    cTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                    >  1:4478102/1‑92 (MQ=255)

                                                                                                                                    cTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTcc    >  2:4801387/1‑140 (MQ=255)

                                                                                                                                    cTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTcc    >  1:9799835/1‑140 (MQ=255)

                                                                                                                                      gAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTcccc  <  2:627977/140‑1 (MQ=255)

                                                                                                                                      gAAGGTCTGGCAGGGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTATGTGGGCGGGGATTTCTGGACTAGCAAAAATTCTTGGTGGTGTGCTGGCGTGGTGAATCCTCGGTAGCATGATTTcccc  <  2:1519461/140‑1 (MQ=255)

GCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTCCCC  >  NZ_CP025268/3183555‑3183828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`