Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,232,860 T→C N170N (AAT→AAC fadH → NADPH‑dependent 2,4‑dienoyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,232,8600TC99.9% 2411.8 / NA 691N170N (AAT→AACfadHNADPH‑dependent 2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (354/336);  minor base A (1/0);  total (355/336)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 691 total aligned reads displayed.

AACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGAC  >  NZ_CP025268/3232723‑3232996
                                                                                                                                         |                                                                                                                                        
 aCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGaa                                                                                                                                       >  1:5494999/1‑140 (MQ=255)
     tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                                                                   >  2:7777903/1‑140 (MQ=255)
     tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                                                                   >  2:873723/1‑140 (MQ=255)
     tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                                                                   >  1:174649/1‑140 (MQ=255)
     tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                                                                   >  1:8046020/1‑140 (MQ=255)
       tCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTg                                                                                                                                 >  1:9437506/1‑140 (MQ=255)
       tCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTg                                                                                                                                 <  1:5033254/140‑1 (MQ=255)
       tCGTGCCCCATGAGTTAAGACATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCATCTGGAGCGGGAGGCAGGATACGACGGTTTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTg                                                                                                                                 <  1:4790166/140‑1 (MQ=255)
        cGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACAGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGAGTGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAACGGTATTTGATCAACGAATTTCTGa                                                                                                                                >  2:10692084/1‑140 (MQ=255)
          tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                                                                                                              >  1:10316093/1‑140 (MQ=255)
          tGCCCCATGAGTTAAGCCATGAAGAGATCCTGAAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGCCg                                                                                                                              <  2:6686478/140‑1 (MQ=255)
            ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCt                                                                                                                            <  2:8363258/140‑1 (MQ=255)
               cATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTgcg                                                                                                                         <  1:7373731/140‑1 (MQ=255)
                 tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGCCGCTGCGCa                                                                                                                       <  1:10155284/140‑1 (MQ=255)
                 tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                                                                                                       >  1:5314027/1‑140 (MQ=255)
                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                     <  2:2151504/140‑1 (MQ=255)
                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATGTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                     <  1:1559428/140‑1 (MQ=255)
                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                  <  2:8616494/140‑1 (MQ=255)
                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                  <  1:10029543/140‑1 (MQ=255)
                       aaGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATc                                                                                                                 >  1:6359821/1‑140 (MQ=255)
                       aaGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATc                                                                                                                 <  1:6113361/140‑1 (MQ=255)
                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                                <  1:1868207/140‑1 (MQ=255)
                          ccATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGc                                                                                                              <  1:3343462/140‑1 (MQ=255)
                           cATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCg                                                                                                             <  2:8111426/140‑1 (MQ=255)
                            aTGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTCGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGt                                                                                                            >  2:4182513/1‑140 (MQ=255)
                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           <  2:2946366/140‑1 (MQ=255)
                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           >  1:1271715/1‑140 (MQ=255)
                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGGGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           >  2:5456891/1‑140 (MQ=255)
                             agAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           <  2:3300289/139‑1 (MQ=255)
                               aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGt                                                                                                         <  1:3195825/140‑1 (MQ=255)
                               aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGt                                                                                                         <  1:2214356/140‑1 (MQ=255)
                               aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGt                                                                                                         >  1:4464896/1‑140 (MQ=255)
                                agagATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTg                                                                                                        <  1:8551515/140‑1 (MQ=255)
                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                    >  1:5170495/1‑140 (MQ=255)
                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                    >  1:6022833/1‑140 (MQ=255)
                                         gCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTggg                                                                                               >  2:7955930/1‑140 (MQ=255)
                                         gCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTggg                                                                                               <  1:6587543/140‑1 (MQ=255)
                                               gATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           <  2:7308902/122‑1 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGACGGGTATTTGATCAACGAATGTCTGACGCTGCGCACCATTCTGCGGTGGGGCCGGTGGGgcggcgg                                                                                        >  2:8918755/1‑139 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                        >  2:3672088/1‑140 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                        >  1:8642172/1‑140 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                        >  2:1028880/1‑140 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                        >  2:5702840/1‑140 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATAAACGAATTTCTGACGCTGCGCACAAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                        >  2:1820356/1‑140 (MQ=255)
                                                aTCGAAAAATTCGCCAGCTGCGCGCCACTGGCGCGGGACGCAGGATACGACGGTGTCGAGGTGATGGGTTCCGAAGGGTATATTATCAACGAATTTCTGACGCTGAGCACCAATCAGCGAAGTGACCAGTGTGGCGgaga                                                                                        >  2:7282691/1‑140 (MQ=255)
                                                              ccGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGAAGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGa                                                                          <  2:1490717/140‑1 (MQ=255)
                                                                  tGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCg                                                                      >  2:9443513/1‑140 (MQ=255)
                                                                  tGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCg                                                                      >  1:3466382/1‑140 (MQ=255)
                                                                        caccTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                                <  1:8970542/137‑1 (MQ=255)
                                                                        cAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                                <  1:7095296/140‑1 (MQ=255)
                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                              <  1:7612704/140‑1 (MQ=255)
                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                              <  1:1412710/140‑1 (MQ=255)
                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                              <  2:492968/140‑1 (MQ=255)
                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                              <  1:6849377/140‑1 (MQ=255)
                                                                              gcgcGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGa                                                          >  2:5048334/1‑140 (MQ=255)
                                                                                 cgGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGCCGCTGCGCACCAATCTGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagt                                                       <  2:6090593/140‑1 (MQ=255)
                                                                                 cgGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagt                                                       <  2:1942227/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTTCCGTAGAAGTAgtgcgtg                                               <  1:8435266/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  2:2793813/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  1:2890583/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  1:10498465/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  1:10857362/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  1:3596957/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  1:7944651/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGGTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  1:9775083/140‑1 (MQ=255)
                                                                                                gacgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGATGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCgtgcggtgcg                                        >  2:9133735/1‑140 (MQ=255)
                                                                                                gacgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCgtgcggtgcg                                        >  1:3745774/1‑140 (MQ=255)
                                                                                                     tgtAGAGGTGCTGGGTTCCGAACGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTTGTGACCAGTGGGGCGGCGATTACAGAAAACGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGCAc                                   >  2:3843235/1‑140 (MQ=255)
                                                                                                     tgtAGAGGTGCGGGGTTGCGAAGGGTATTTGATCAACGTAGTGCTGACGCTGCGCACGATTGTGCGGAGTGCCCCGGGGGGCGGCGATTCCCGCGCGCGGATGCGATTTGCCGTAGAAGTGGTGGGGGCGGTGGGCGagc                                   >  2:4730876/1‑138 (MQ=255)
                                                                                                     tgtAGAGGTGATGGGTTCCGACGGGTAATTGATCAACGAATTTCTCACGCTGCGGACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCTACCGGATGCGATGTGCCGTAGAGGTTGTGCGTGCGGTGCGCGaag                                   >  2:10040687/1‑139 (MQ=255)
                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                   >  1:2048326/1‑140 (MQ=255)
                                                                                                      gtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAcg                                  >  2:165071/1‑140 (MQ=255)
                                                                                                      gtAGAGGTGATGGGGTCCGACTGGTAGTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCTATTTGCAGTAGAAGTAGTGAGTGCGGTGCGCGAAcg                                  >  2:9863571/1‑140 (MQ=255)
                                                                                                             tGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGc                           >  2:11750/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                     <  1:9287650/48‑1 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCCGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGTGCGCGTCGGctg                         >  2:10428154/1‑138 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGCTTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGTACGCGTCGGCac                         >  2:3411748/1‑139 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCTACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  2:7599544/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  1:5910882/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         <  1:883647/140‑1 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  1:9722991/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  2:7091509/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  2:593269/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  2:358852/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  2:5263621/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGGGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGGCGGCaa                         >  2:3190415/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAAGTTCTGACGCTGCGCACCAATCCGCGTAGTGACCAGTGGGGCGGCGATTACCGCACCCGGATGCGATGTGCCGGAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGcta                         >  2:3953774/1‑138 (MQ=255)
                                                                                                                 gggTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAAACGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACg                       >  2:9664513/1‑140 (MQ=255)
                                                                                                                  ggTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTTCGCACCAATCAGCGTAGTGACCAGTGGGGCGTCGATTACCTCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGAGGTTAGATAACGAGTCGGCataga                      <  2:2370426/140‑5 (MQ=255)
                                                                                                                   gTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGAc                     <  1:4113182/140‑1 (MQ=255)
                                                                                                                   gTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGAc                     <  2:1293574/140‑1 (MQ=255)
                                                                                                                    ttCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACt                    >  2:2635480/1‑140 (MQ=255)
                                                                                                                    ttCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACt                    >  2:10628130/1‑140 (MQ=255)
                                                                                                                      ccGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTc                  >  2:4546955/1‑140 (MQ=255)
                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                 <  1:2641338/140‑1 (MQ=255)
                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                 <  1:8142777/140‑1 (MQ=255)
                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                 <  2:629617/140‑1 (MQ=255)
                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                 <  2:4464896/140‑1 (MQ=255)
                                                                                                                           gggTATTTGATCAACGAATGTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCattat             <  2:6574201/140‑1 (MQ=255)
                                                                                                                                      cAACGAATTTATGACGCTGCGCACCAATCAGCGTAGTGAGCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGGGCGGTGCGCGTCCGCGGCGGCCACGAGGTCTTTATTTTCTAGCGAc  >  2:6891250/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGAC  >  NZ_CP025268/3232723‑3232996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: