Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,270,287	A→G	*396* (TAA→TAG)	yhaC →	YhaC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,270,287	0	A	G	100.0%	218.8 / NA	65	*396* (TAA→TAG)	yhaC	YhaC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/15);  total (50/15)

CGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGC  >  NZ_CP025268/3270152‑3270422                                                                                                                                        |                                                                                                                                        cGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACtt                                                                                                                                     >  2:7749054/1‑140 (MQ=255)       aGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAc                                                                                                                               >  2:3013068/1‑140 (MQ=255)       aGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAc                                                                                                                               <  1:4677141/140‑1 (MQ=255)        gACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAct                                                                                                                              >  1:2564238/1‑140 (MQ=255)              aaTTCTCCGGACAATGGCTTTGAGGTCGCGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTAc                                                                                                                        <  1:7749055/140‑1 (MQ=255)                     cGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCt                                                                                                                 <  2:1209876/140‑1 (MQ=255)                     cGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCt                                                                                                                 <  2:8072101/140‑1 (MQ=255)                                        cGGGGAATATCACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTCAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTACTACCGTAGata                                                                                              >  2:7098615/1‑140 (MQ=255)                                        cGGGGAATAAAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATAAGTATGCTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCGTACAAAGTCTATTATTAAAACCGTAGata                                                                                              >  2:7098677/1‑140 (MQ=255)                                                       atatGAAGCATATAACTGAGAGAAGCAATATTTTACCAGAGAGGACTATACGTCTGATTACGCCCTTTTTAATGCAATATAGACTTAATAATTTGTTTCATAGTGTATGTTTCTTTCCGTTGATATTTATTAATCTTAcc                                                                               >  2:8354807/1‑140 (MQ=255)                                                       atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGGGGACTATACGTCTGATTACGTCATGTTAAATGCAATATAGAGTTAATAACTTCTGACAAAGTCTATTGTTAATTCGGTAGATAGTTATTCATATTTcc                                                                               >  2:8903789/1‑140 (MQ=255)                                                       atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                               <  1:1897319/140‑1 (MQ=255)                                                       atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                               <  2:3900638/140‑1 (MQ=255)                                                         atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAGATTTATTCATCTTACCCt                                                                             >  1:8055275/1‑140 (MQ=255)                                                                 tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGAc                                                                                                                                       >  2:588406/1‑74 (MQ=255)                                                                 tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGAc                                                                                                                                       <  1:588406/74‑1 (MQ=255)                                                                 tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCa                                                                     >  2:5418049/1‑140 (MQ=255)                                                                 tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCa                                                                     >  1:5612020/1‑140 (MQ=255)                                                                 tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCa                                                                     >  2:2523662/1‑140 (MQ=255)                                                                 tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCa                                                                     >  1:2250153/1‑140 (MQ=255)                                                                   taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCaaa                                                                   >  2:4435866/1‑140 (MQ=255)                                                                   taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCaaa                                                                   <  1:3013068/140‑1 (MQ=255)                                                                     aCTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAg                                                                 >  1:9879179/1‑140 (MQ=255)                                                                         agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaa                                                                                                                                   >  1:8328437/1‑70 (MQ=255)                                                                         agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaa                                                                                                                                   <  2:8328436/70‑1 (MQ=255)                                                                         agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAc                                                                                                                               >  2:10486760/1‑74 (MQ=255)                                                                         agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAc                                                                                                                               <  1:10486761/74‑1 (MQ=255)                                                                         agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaa                                                             >  1:4335622/1‑140 (MQ=255)                                                                          gagaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaaa                                                            >  2:651963/1‑140 (MQ=255)                                                                           agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaaaa                                                           >  1:4726360/1‑140 (MQ=255)                                                                              aGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTc                                                        >  1:6773086/1‑140 (MQ=255)                                                                               gCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaa                                                                                                                                   >  1:6482056/1‑64 (MQ=255)                                                                               gCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaa                                                                                                                                   <  2:6482055/64‑1 (MQ=255)                                                                                cAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCAc                                                      >  2:5431619/1‑140 (MQ=255)                                                                                cAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCAc                                                      >  2:2041940/1‑140 (MQ=255)                                                                                  atatTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAc                                                                                                                               >  1:1113763/1‑65 (MQ=255)                                                                                  atatTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAc                                                                                                                               <  2:1113763/65‑1 (MQ=255)                                                                                     ttttACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATTCAATATAGACTTAATAACTTCTTACAAAGTATATTATTAATACCGTAGATATTTATTCATCTTACCCGGTGCACCAAAAGCAAAAACTCACTGAcg                                                 >  2:1067137/1‑140 (MQ=255)                                                                                             gagagGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTg                                         >  2:2813032/1‑140 (MQ=255)                                                                                               gagGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGaa                                       >  1:4536513/1‑140 (MQ=255)                                                                                                  gACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACt                                    >  2:9765036/1‑140 (MQ=255)                                                                                                          gTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCaa                            >  2:5405510/1‑140 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTtaa                                                                                                                                   >  1:456595/1‑35 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTtaa                                                                                                                                   <  2:456595/35‑1 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACa                                                                                                                       >  2:7453490/1‑47 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACa                                                                                                                       <  1:7453491/47‑1 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:8158418/1‑140 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:4393607/1‑140 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:591773/1‑140 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:9996773/1‑140 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGACTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:10045592/1‑140 (MQ=255)                                                                                                             tGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCg                         >  1:7430784/1‑140 (MQ=255)                                                                                                             tGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCg                         >  1:476894/1‑140 (MQ=255)                                                                                                             tGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCg                         >  1:477334/1‑140 (MQ=255)                                                                                                               aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  1:6024311/1‑140 (MQ=255)                                                                                                               aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  2:2626686/1‑140 (MQ=255)                                                                                                                 tACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaa                     >  2:3210973/1‑140 (MQ=255)                                                                                                                 tACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGACTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaa                     >  1:1572129/1‑140 (MQ=255)                                                                                                                     aCCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCACAAATg                 >  2:4359249/1‑140 (MQ=255)                                                                                                                       cTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGcc               >  1:5246693/1‑140 (MQ=255)                                                                                                                        ttttAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCt              >  1:8517761/1‑140 (MQ=255)                                                                                                                         tttAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTg             <  2:4726360/140‑1 (MQ=255)                                                                                                                         tttAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAA‑TCACGTAAGCGGGGTTGAAACAGATGGCCACCGCAAAAATGCCAGa            >  2:2995709/1‑140 (MQ=255)                                                                                                                          ttAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGa            >  2:2504401/1‑140 (MQ=255)                                                                                                                                    tataGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGc  >  2:8357508/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGC  >  NZ_CP025268/3270152‑3270422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑