Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,272,617 C→T A276T (GCG→ACG)  garR ← 2‑hydroxy‑3‑oxopropionate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,272,6170CT99.4% 1872.0 / NA 496A276T (GCG→ACG) garR2‑hydroxy‑3‑oxopropionate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (241/250);  minor base G (1/2);  total (244/252)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 511 total aligned reads displayed.

CCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCGGC  >  NZ_CP025268/3272480‑3272753
                                                                                                                                         |                                                                                                                                        
  ggAGGGCGTTTCTCGTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACTTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCt                                                                                                                                      <  2:8019276/140‑1 (MQ=255)
   gAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTa                                                                                                                                     <  1:8804918/140‑1 (MQ=255)
    aGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaa                                                                                                                                    >  2:7384105/1‑140 (MQ=255)
    aGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaa                                                                                                                                    >  1:8919122/1‑140 (MQ=255)
    aGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaa                                                                                                                                    >  1:6261406/1‑140 (MQ=255)
    aGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaa                                                                                                                                    <  1:2899097/140‑1 (MQ=255)
        cGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTGTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCa                                                                                                                                >  2:165761/1‑140 (MQ=255)
          tttCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATc                                                                                                                              >  2:3285584/1‑140 (MQ=255)
                  aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                      <  2:5584772/140‑1 (MQ=255)
                  aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                      <  1:10713750/140‑1 (MQ=255)
                  aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                      <  1:827441/140‑1 (MQ=255)
                  aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                      <  1:7459038/140‑1 (MQ=255)
                  aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                      >  1:6569241/1‑140 (MQ=255)
                  aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCAATAACGAGTAACTTCGACTTTCGCCAGTTTTTCGGAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                      <  1:6816763/140‑1 (MQ=255)
                     cGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTGTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTg                                                                                                                   <  1:2654000/140‑1 (MQ=255)
                     cGGCCTGGAGTCCAGGCCCGGATTGCAATCCGCCAGCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTg                                                                                                                   <  2:9441045/140‑1 (MQ=255)
                       gCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                                                                                                                 <  1:176336/140‑1 (MQ=255)
                        ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                                >  1:3652747/1‑140 (MQ=255)
                        ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                                >  1:6210086/1‑140 (MQ=255)
                        ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                                >  1:867686/1‑140 (MQ=255)
                        ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                                >  1:10015307/1‑140 (MQ=255)
                        ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTACGACTTTCGCCAGTTTTACGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                                >  2:6515532/1‑140 (MQ=255)
                          tGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGc                                                                                                              <  1:9702768/140‑1 (MQ=255)
                                  aGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtctc                                                                                                      >  1:1389087/1‑140 (MQ=255)
                                  aGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtctc                                                                                                      >  1:5434948/1‑140 (MQ=255)
                                  aGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGGAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtctc                                                                                                      >  2:7245324/1‑140 (MQ=255)
                                    gCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCa                                                                                                    >  2:187623/1‑140 (MQ=255)
                                      ccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATa                                                                                                  >  1:3354166/1‑140 (MQ=255)
                                                  aCGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCtgtg                                                                                      <  2:1457610/140‑1 (MQ=255)
                                                   cGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGa                                                                                     >  2:2769413/1‑140 (MQ=255)
                                                      cATCCTGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCg                                                                                  >  1:3746514/1‑140 (MQ=255)
                                                        tCCGGGCACGACGTCATTAACGAGTAACTTCGAGTTTCGGCAGTGTTTCGTAGTAGCACGCGAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATATCCGCAGCTGTGAGCGGc                                                                                >  2:3081756/1‑140 (MQ=255)
                                                        tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCTCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTTGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGc                                                                                >  2:2424540/1‑140 (MQ=255)
                                                        tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGc                                                                                >  1:4270994/1‑140 (MQ=255)
                                                        tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGc                                                                                >  2:5438039/1‑140 (MQ=255)
                                                        tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGc                                                                                >  1:1641106/1‑140 (MQ=255)
                                                        tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGc                                                                                >  2:7755623/1‑140 (MQ=255)
                                                                cgacgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTgc                                                                        >  1:3911555/1‑140 (MQ=255)
                                                                    gTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCg                                                                    >  1:220271/1‑140 (MQ=255)
                                                                      cATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                                                  <  1:9446242/140‑1 (MQ=255)
                                                                      cATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                                                  <  2:827151/140‑1 (MQ=255)
                                                                      cATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                                                  <  1:2141889/140‑1 (MQ=255)
                                                                         tAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGcc                                                               <  2:9657583/140‑1 (MQ=255)
                                                                         tAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGcc                                                               >  1:9832631/1‑140 (MQ=255)
                                                                           aCGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                             <  2:3455822/140‑1 (MQ=255)
                                                                           aCGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                             <  1:10181293/140‑1 (MQ=255)
                                                                             gAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTga                                                           <  2:7449353/140‑1 (MQ=255)
                                                                              aGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTgag                                                          <  1:322617/140‑1 (MQ=255)
                                                                               gTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTgaga                                                         >  1:5978785/1‑140 (MQ=255)
                                                                                tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGaa                                                        >  1:4578832/1‑140 (MQ=255)
                                                                                  aCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCATAAACAATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGACGt                                                      >  2:6177681/1‑140 (MQ=255)
                                                                                       gACTTTCGCCAGTTTTTCGGAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCa                                                 <  2:3244843/140‑1 (MQ=255)
                                                                                        aCTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAg                                                >  2:9022373/1‑140 (MQ=255)
                                                                                        aCTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAg                                                >  1:115192/1‑140 (MQ=255)
                                                                                            tCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                             <  2:8122497/123‑1 (MQ=255)
                                                                                            tCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCa                                            >  2:3993659/1‑140 (MQ=255)
                                                                                                  gTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                                      <  1:3038738/140‑1 (MQ=255)
                                                                                                  gTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                                      <  2:6966254/140‑1 (MQ=255)
                                                                                                  gTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                                      <  2:4613634/140‑1 (MQ=255)
                                                                                                   tttttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa                                     <  1:8014369/140‑1 (MQ=255)
                                                                                                   ttattCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGACCGGCAGTTGTGCGCCGACGCCCTGAGAAGTATCCAGCGCATTCGCCa                                     >  2:371300/4‑140 (MQ=255)
                                                                                                      ttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                  <  2:4400640/140‑1 (MQ=255)
                                                                                                      ttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                  <  1:634340/140‑1 (MQ=255)
                                                                                                      ttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                  <  2:4271957/140‑1 (MQ=255)
                                                                                                      ttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                  <  2:3902442/140‑1 (MQ=255)
                                                                                                      ttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                  <  2:1303782/140‑1 (MQ=255)
                                                                                                         gtagtaGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCTGAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCt                               <  2:10537133/140‑1 (MQ=255)
                                                                                                           agtagCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                             <  1:7684626/140‑1 (MQ=255)
                                                                                                           agtagCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                             <  1:4697230/140‑1 (MQ=255)
                                                                                                                cACGCCTGGGCGCTATGATCCGACGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                        <  2:5211953/140‑1 (MQ=255)
                                                                                                                cACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCTCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                        <  2:10849395/140‑1 (MQ=255)
                                                                                                                cACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                        <  2:10817189/140‑1 (MQ=255)
                                                                                                                cACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                        <  2:8873084/140‑1 (MQ=255)
                                                                                                                  cGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGTCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCa                      >  2:10174373/1‑140 (MQ=255)
                                                                                                                  cGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCa                      <  1:190105/140‑1 (MQ=255)
                                                                                                                  cGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCa                      <  1:4893959/140‑1 (MQ=255)
                                                                                                                  cGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCa                      >  2:5871659/1‑140 (MQ=255)
                                                                                                                  cGCCAGGGCGCTATGATCC‑CCGTTCCTAAACCATATTCACGCAGTGCCGGCATCAACTCCCAAACCCCCGCTGTGAGCCGCAGGTGTGCGCCGCCGCCGGGATAAGTATCCCGCGCATTCGCCCGCTCCATAATATGCAg                     >  1:3988999/1‑140 (MQ=255)
                                                                                                                    ccAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGa                    >  2:6710273/1‑140 (MQ=255)
                                                                                                                     cAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGAt                   <  1:5232146/140‑1 (MQ=255)
                                                                                                                       gggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCa                 >  1:8547401/1‑140 (MQ=255)
                                                                                                                       gggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCa                 >  1:1546563/1‑140 (MQ=255)
                                                                                                                       gggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCa                 >  1:10497663/1‑140 (MQ=255)
                                                                                                                        ggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCaa                >  1:4084114/1‑140 (MQ=255)
                                                                                                                          cgcTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATa              >  1:2537117/1‑140 (MQ=255)
                                                                                                                           gcTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATAc             >  1:5886893/1‑140 (MQ=255)
                                                                                                                             tATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACgg           >  2:5069199/1‑140 (MQ=255)
                                                                                                                             tATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGAATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGTAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATTCgg           >  2:4770611/1‑140 (MQ=255)
                                                                                                                               tGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCAGTCGCCCGATCCTTAATATGCAGATCAATACGGaa         >  2:1775221/1‑140 (MQ=255)
                                                                                                                                 aTCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGc       <  2:4968894/140‑1 (MQ=255)
                                                                                                                                 aTCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGc       <  2:819180/140‑1 (MQ=255)
                                                                                                                                 aTCCGCCGTGCCTAACCCATCTGCTCGCAGTGCCTGCAGCAGCTCCATCCCCGCAGCTGGGAGCGGCCGTTGTGCGCCGCCGCCGTGAGAAGGATCCCGCGCAGTCGCCAGATCCTTAATATGCAGATCAATACGGactc       <  2:9786388/140‑4 (MQ=255)
                                                                                                                                  tCCGCCGTTCCTAACCCATCTGCTCGCAGTGCCTGCATCATCTCCATTACCGCAGCTGTGAGCTGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCTCATTCGCCAGATCCTTAATATTCAGATAAATACGGAAGcc      <  2:10032957/140‑1 (MQ=255)
                                                                                                                                  tCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGcc      <  2:5938674/140‑1 (MQ=255)
                                                                                                                                  tCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGcc      <  1:9183869/140‑1 (MQ=255)
                                                                                                                                    cgccgtTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg    >  2:6351898/1‑140 (MQ=255)
                                                                                                                                    cgccgtTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg    >  2:2213170/1‑140 (MQ=255)
                                                                                                                                    cgccgtTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg    >  2:10395195/1‑140 (MQ=255)
                                                                                                                                    cgccgtTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg    >  1:7145356/1‑140 (MQ=255)
                                                                                                                                    cgccgtTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg    >  1:10290265/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCGGC  >  NZ_CP025268/3272480‑3272753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑