Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,278,552 T→C I267V (ATC→GTC)  agaR ← aga operon transcriptional regulator AgaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,278,5520TC99.8% 1709.6 / NA 490I267V (ATC→GTC) agaRaga operon transcriptional regulator AgaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (222/267);  minor base A (0/1);  total (222/268)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 490 total aligned reads displayed.

AATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCG  >  NZ_CP025268/3278417‑3278688
                                                                                                                                       |                                                                                                                                        
aaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGGTCACAATGCCCGCCGAACGCAGTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGa                                                                                                                                      >  1:2697267/1‑140 (MQ=255)
  tGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAAt                                                                                                                                    >  1:427192/1‑140 (MQ=255)
      aaacaGAGGACATCCTCCTGCCGACTGGGAAACACTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCCCt                                                                                                                                <  1:2456096/140‑1 (MQ=255)
       aaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACtt                                                                                                                               <  1:7011823/140‑1 (MQ=255)
       aaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACtt                                                                                                                               >  1:1888088/1‑140 (MQ=255)
            agGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAAcc                                                                                                                          >  1:10002331/1‑140 (MQ=255)
              gACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAAcccc                                                                                                                        >  1:6361828/1‑140 (MQ=255)
              gACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAAcccc                                                                                                                        >  1:22533/1‑140 (MQ=255)
               aCATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCa                                                                                                                       <  2:4335190/140‑1 (MQ=255)
                   cctcctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCt                                                                                                                   >  2:1859357/1‑140 (MQ=255)
                   cctcctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCAGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCt                                                                                                                   >  1:6134636/1‑140 (MQ=255)
                      cctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTc                                                                                                                <  2:4838253/140‑1 (MQ=255)
                       ctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCg                                                                                                               <  2:23432/140‑1 (MQ=255)
                        tGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGc                                                                                                              >  1:789369/1‑140 (MQ=255)
                          ccGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                            >  2:5403569/1‑140 (MQ=255)
                          ccGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                            <  1:8062865/140‑1 (MQ=255)
                          ccGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                            <  1:10339218/140‑1 (MQ=255)
                             aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTcc                                                                                                         >  2:1144684/1‑140 (MQ=255)
                             aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTcc                                                                                                         >  1:1011541/1‑140 (MQ=255)
                                    aaCCCTCACCCGAGAAACAGAAGAATCCCATTGATGGTGTTCACAATGCCCGCCGTACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                                  <  1:4815986/140‑1 (MQ=255)
                                    aaCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                                  <  1:2150726/140‑1 (MQ=255)
                                      cccTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt                                                                                                <  1:3596617/140‑1 (MQ=255)
                                         tCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATc                                                                                             <  2:1025844/140‑1 (MQ=255)
                                         tCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATc                                                                                             <  1:8583213/140‑1 (MQ=255)
                                           aCCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCg                                                                                           >  2:10786831/1‑140 (MQ=255)
                                           aCCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCg                                                                                           >  1:2829314/1‑140 (MQ=255)
                                           aCCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCg                                                                                           >  2:3958528/1‑140 (MQ=255)
                                                 gaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCCGAATCACTTCAACCCCCGCCTTTCGCAGTCCTTCCAGGCGATCCGCAGGaa                                                                                     >  2:237685/1‑140 (MQ=255)
                                                   aaCAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATg                                                                                   <  1:3242858/140‑1 (MQ=255)
                                                   aaCAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATg                                                                                   <  2:5093249/140‑1 (MQ=255)
                                                   aaCAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATg                                                                                   <  1:4660429/140‑1 (MQ=255)
                                                     ctgaagatTCCCATTGATGTTGTTCTCAATGAACGCCGATCGCTTTCGTTTAGACCAGCTGTCATCCTTTCTCTCTCCCCGACCAGATTCAATTCAACCGCAGCCTTTCGCAGTCCTTCCATGATATCCGCATGAATGcc                                                                                 <  2:523584/138‑1 (MQ=255)
                                                     cAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                                 <  2:134459/140‑1 (MQ=255)
                                                          gaaACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                            <  1:2533873/140‑1 (MQ=255)
                                                          gaaACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                            <  2:2694082/140‑1 (MQ=255)
                                                          gaaACCCATTGATGGAGTTCACAATGCCCGCCGAACTCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                            <  2:7201347/140‑1 (MQ=255)
                                                           aaaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCttcttc                                                                           <  1:9049209/140‑1 (MQ=255)
                                                           aaaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcatc                                                                           <  1:3774165/140‑1 (MQ=255)
                                                           aaaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcatc                                                                           <  2:3050850/140‑1 (MQ=255)
                                                              cccATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaac                                                                        <  2:6539665/140‑1 (MQ=255)
                                                               ccATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                       <  2:6322663/140‑1 (MQ=255)
                                                                    gATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                                  <  2:7838926/140‑1 (MQ=255)
                                                                    gATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                                  <  1:6550646/140‑1 (MQ=255)
                                                                    gATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                                  <  2:1474634/140‑1 (MQ=255)
                                                                    gATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                                  <  2:4671945/140‑1 (MQ=255)
                                                                    gATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                                  <  1:8649927/140‑1 (MQ=255)
                                                                        gtgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACACTGATCATg                                                              <  2:2899520/140‑1 (MQ=255)
                                                                        gtgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATg                                                              >  1:1287472/1‑140 (MQ=255)
                                                                         tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGt                                                             >  1:10647820/1‑140 (MQ=255)
                                                                           ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCGTCAACAATGATCATGTCg                                                           >  2:10040891/1‑140 (MQ=255)
                                                                           ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATACTTTCTCACTCACCGACCCGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAAAGCCATCATCAACACTGATCATGTCg                                                           >  2:6947446/1‑140 (MQ=255)
                                                                            tCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                          <  1:8344402/140‑1 (MQ=255)
                                                                            tCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                          <  2:4977339/140‑1 (MQ=255)
                                                                             cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                         <  2:1701806/140‑1 (MQ=255)
                                                                             cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                         <  1:9584828/140‑1 (MQ=255)
                                                                             cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                         <  2:5634248/140‑1 (MQ=255)
                                                                             cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                         <  1:9768910/140‑1 (MQ=255)
                                                                                aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                      <  1:7385166/140‑1 (MQ=255)
                                                                                aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                      <  2:2544749/140‑1 (MQ=255)
                                                                                aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                      <  2:3554090/140‑1 (MQ=255)
                                                                                 aTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGt                                                     >  1:1545192/1‑140 (MQ=255)
                                                                                  tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                            >  1:5898283/1‑116 (MQ=255)
                                                                                  tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                          <  1:9197618/134‑1 (MQ=255)
                                                                                  tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                    <  1:4351090/140‑1 (MQ=255)
                                                                                  tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                    <  2:10647819/140‑1 (MQ=255)
                                                                                  tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                    >  2:3793327/1‑140 (MQ=255)
                                                                                   gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                                  >  1:4561485/1‑125 (MQ=255)
                                                                                   gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTg                                                   >  2:8554017/1‑140 (MQ=255)
                                                                                   gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTg                                                   >  1:7466343/1‑140 (MQ=255)
                                                                                   gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTg                                                   >  2:7058641/1‑140 (MQ=255)
                                                                                     ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAg                                                 <  2:8003817/140‑1 (MQ=255)
                                                                                     ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAg                                                 >  1:9650331/1‑140 (MQ=255)
                                                                                     ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAg                                                 <  1:3047430/140‑1 (MQ=255)
                                                                                     ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAg                                                 <  1:8197075/140‑1 (MQ=255)
                                                                                      cgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGt                                                <  2:9284679/140‑1 (MQ=255)
                                                                                        ccgAACGCAGTCGTGTTGACCAGCGGCCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCNTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACNTTGAGTAt                                              <  1:5821808/140‑1 (MQ=255)
                                                                                          gAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCa                                            >  1:6047053/1‑140 (MQ=255)
                                                                                          gAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTACATCAACAATGAACATGTCGATACGTTGAGTATCa                                            >  2:5734839/1‑140 (MQ=255)
                                                                                           aaCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                           <  2:6148871/140‑1 (MQ=255)
                                                                                           aaCGCATTCGTGTGGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                           <  1:7582484/140‑1 (MQ=255)
                                                                                                 ttCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGAATCAATGATCt                                     >  2:9024508/1‑140 (MQ=255)
                                                                                                     tgtTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                       <  1:5277902/102‑1 (MQ=255)
                                                                                                        tGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTaa                              >  1:3428551/1‑140 (MQ=255)
                                                                                                               cGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTggca                       >  2:7891143/1‑138 (MQ=255)
                                                                                                               cGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGaa                       >  2:4240892/1‑140 (MQ=255)
                                                                                                               cGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGaa                       >  1:10293416/1‑140 (MQ=255)
                                                                                                               cGGGCATCCTTTCTCACTCCCCGACCAGAATCACATCAACCCCACCCTTACTCAGACCTTCCCGGCAATCCGCAGGAATGCCATCATCAACAAAGATCATGTCGATACCTTGAGTATCAATGATCTTATGTAAACTgcac                       >  2:8697239/1‑137 (MQ=255)
                                                                                                               cGGGCATCCGTTCTCAATCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGaa                       >  2:3154118/1‑140 (MQ=255)
                                                                                                                    aTCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGAtt                  >  1:10779443/1‑140 (MQ=255)
                                                                                                                      ccTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGa                >  1:7163162/1‑140 (MQ=255)
                                                                                                                        tttCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAAc              <  2:2108452/140‑1 (MQ=255)
                                                                                                                            tcACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTAc          >  2:9885305/1‑140 (MQ=255)
                                                                                                                              aCTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTg        <  2:7425142/140‑1 (MQ=255)
                                                                                                                                tCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGa      >  2:8322118/1‑140 (MQ=255)
                                                                                                                                tCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGa      <  1:1762608/140‑1 (MQ=255)
                                                                                                                                tCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGa      <  1:5074225/140‑1 (MQ=255)
                                                                                                                                 ccccGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGaa     >  1:5519811/1‑140 (MQ=255)
                                                                                                                                 ccccGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGaa     >  1:670780/1‑140 (MQ=255)
                                                                                                                                 ccccGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGaa     >  1:3907925/1‑140 (MQ=255)
                                                                                                                                 ccccGACCAGAATCACTACAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGATTAGTATCAATGATCTTATGTATACTGGAACGTTTGAACTTACTggga     >  2:9538103/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                        
AATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCG  >  NZ_CP025268/3278417‑3278688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: