Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,483,653 C→T P560L (CCG→CTG)  yheS → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,483,6530CT99.2% 1830.3 / NA 476P560L (CCG→CTG) yheSABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (271/200);  minor base G (1/3);  total (273/203)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 494 total aligned reads displayed.

TTATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAACA  >  NZ_CP025268/3483516‑3483788
                                                                                                                                         |                                                                                                                                       
 tATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACtgcg                                                                                                                                      >  1:3838347/1‑140 (MQ=255)
 tATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACtgcg                                                                                                                                      >  2:4339331/1‑140 (MQ=255)
   tCAACCGTGGTTGAGCGACGTCCAAAAGCAGGAAAACCAGACCGACGAAGCGCCACAAGAGACTGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGGTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                    >  2:3891932/1‑140 (MQ=255)
   tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                    >  2:8627017/1‑140 (MQ=255)
     aaCAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTaaa                                                                                                                                  >  2:2142578/1‑140 (MQ=255)
        aGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAagag                                                                                                                               >  1:7610426/1‑140 (MQ=255)
          tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                             >  1:4336202/1‑140 (MQ=255)
          tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                             >  1:2261538/1‑140 (MQ=255)
                aGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCg                                                                                                                       <  1:250559/140‑1 (MQ=255)
                 gCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGt                                                                                                                      >  2:7697595/1‑140 (MQ=255)
                 gCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGt                                                                                                                      >  2:6867016/1‑140 (MQ=255)
                  cGACGTACAAAAGCAGGAAAACCAGACCGACGAATCGCCAAAAGAGAATCCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGACGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTc                                                                                                                     >  1:8299863/1‑140 (MQ=255)
                    aCGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTg                                                                                                                   >  1:4525614/1‑140 (MQ=255)
                    aCGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAAAGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACAGCGTAAAGAGATTGCCCGTCTg                                                                                                                   >  2:1092779/1‑140 (MQ=255)
                    aCGTACAAAAGCACGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCAGAGCTGCGTGCGCAAACCCAGCCACTGCGAAAAGAGATTGCCCGTCTg                                                                                                                   >  2:10001436/1‑140 (MQ=255)
                        aCAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGaaa                                                                                                               <  2:2627818/140‑1 (MQ=255)
                            aaGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAaga                                                                                                           >  2:4720323/1‑140 (MQ=255)
                               cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                        >  1:9528479/1‑140 (MQ=255)
                               cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                        >  1:2034886/1‑140 (MQ=255)
                               cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                        >  2:10209361/1‑140 (MQ=255)
                               cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                        >  2:2318527/1‑140 (MQ=255)
                                 ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                      >  1:6778860/1‑140 (MQ=255)
                                 ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                      >  2:3132749/1‑140 (MQ=255)
                                 ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                      >  1:3032438/1‑140 (MQ=255)
                                 ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                      >  2:5735044/1‑140 (MQ=255)
                                 ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                      >  2:5999118/1‑140 (MQ=255)
                                  gAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGATCAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGACGCTGAGCTGCGTGCGCAAACCCAGCCAGTGCGTAGAGAGATTGCCCGTCTGGAAATAGTGTTGga                                                                                                     >  2:1184279/1‑140 (MQ=255)
                                        cAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCt                                                                                               >  2:4060892/1‑140 (MQ=255)
                                          gACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGa                                                                                             >  1:8748524/1‑140 (MQ=255)
                                              gacgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcgc                                                                                         >  1:729413/1‑140 (MQ=255)
                                               acgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcgcg                                                                                        >  1:5948381/1‑140 (MQ=255)
                                                 gaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCa                                                                                      <  1:3335752/140‑1 (MQ=255)
                                                       ccAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGNGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgc                                                                                >  1:5804944/1‑140 (MQ=255)
                                                       ccAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgc                                                                                >  1:8388197/1‑140 (MQ=255)
                                                         aaaaGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgcgc                                                                              <  1:5056973/140‑1 (MQ=255)
                                                              agaATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCg                                                                         <  2:1938581/140‑1 (MQ=255)
                                                               gaATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCgg                                                                        >  1:3606256/1‑140 (MQ=255)
                                                                  tGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAag                                                                     >  2:8702098/1‑140 (MQ=255)
                                                                  tGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAag                                                                     >  2:4238534/1‑140 (MQ=255)
                                                                    cGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAagag                                                                   >  1:4011485/1‑140 (MQ=255)
                                                                     gAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAgaga                                                                  <  1:5730047/140‑1 (MQ=255)
                                                                      aaCAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGaa                                                                 >  1:5588321/1‑140 (MQ=255)
                                                                      aaCAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGaa                                                                 >  2:5375865/1‑140 (MQ=255)
                                                                          gcgcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGACACTc                                                             >  1:1241778/1‑140 (MQ=255)
                                                                            gcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCgg                                                           >  1:6297332/1‑140 (MQ=255)
                                                                             cccAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGc                                                          >  1:10762934/1‑140 (MQ=255)
                                                                               cAGGCACGTACAGATCAGCAGCGTCGGGAAGCGGAGCTGCGTGCGCAAACCCAGCCACTGAGTAAAGAGCGTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGa                                                        <  2:7524592/140‑1 (MQ=255)
                                                                                    ccGTACAGATCAGCAGCGTCGGGAAGCTGCGCAGCGTGCCCCACCCCACCCACGGCGGAAAGCGAGTGCCCGTCCGCAACAAGAGATGGAGAAGCGGAACGCGCAACTGGCGCAGGCGGCAGAGAAACTCGGCGACACCg                                                   <  2:5182948/139‑1 (MQ=255)
                                                                                    aCGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                   <  2:10379140/140‑1 (MQ=255)
                                                                                    aCGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                   <  1:1866749/140‑1 (MQ=255)
                                                                                     cgaaaaGATCAGACGCGACGGGAAGCAGAGCTGCGTCCGCAAACCCACCCACTGCGTAAACAGCTCGCCCGTCTGGAAAACGAGATCGAGCACCTGAACCCGCAACAGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  <  2:452886/137‑1 (MQ=255)
                                                                                     cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  <  2:2357147/140‑1 (MQ=255)
                                                                                     cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  >  1:1033635/1‑140 (MQ=255)
                                                                                     cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  <  2:1457736/140‑1 (MQ=255)
                                                                                     cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  <  2:10731056/140‑1 (MQ=255)
                                                                                     cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  <  1:3116547/140‑1 (MQ=255)
                                                                                     cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  <  1:6943753/140‑1 (MQ=255)
                                                                                     cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  <  1:4729294/140‑1 (MQ=255)
                                                                                     cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                  <  1:5902224/140‑1 (MQ=255)
                                                                                        aaaGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACt                                               >  2:2960959/1‑140 (MQ=255)
                                                                                        aaaGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACt                                               >  2:7393454/1‑140 (MQ=255)
                                                                                          aGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGt                                             <  1:10595875/140‑1 (MQ=255)
                                                                                            aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTAt                                           >  1:5478729/1‑140 (MQ=255)
                                                                                               aGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCTCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                        >  2:9186613/1‑140 (MQ=255)
                                                                                               aGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                        <  2:7893856/140‑1 (MQ=255)
                                                                                               aGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                        <  1:10004897/140‑1 (MQ=255)
                                                                                                gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAcc                                       >  1:9355444/1‑140 (MQ=255)
                                                                                                gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAcc                                       >  1:7466359/1‑140 (MQ=255)
                                                                                                 aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGt                                             <  2:163606/133‑1 (MQ=255)
                                                                                                 aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGt                                             >  1:163606/1‑133 (MQ=255)
                                                                                                  aGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCag                                     >  1:2849897/1‑140 (MQ=255)
                                                                                                    cGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCagag                                   <  1:9033545/140‑1 (MQ=255)
                                                                                                    cGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTATGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCagag                                   <  1:408909/140‑1 (MQ=255)
                                                                                                      tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                        <  2:3477360/133‑1 (MQ=255)
                                                                                                      tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                        >  1:3477360/1‑133 (MQ=255)
                                                                                                      tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGcc                                 >  2:10477478/1‑140 (MQ=255)
                                                                                                      tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGcc                                 >  1:6610785/1‑140 (MQ=255)
                                                                                                       cGGGACGCGGCGCTGCGTGCGCCCCCCCAGCCCCTGCGTAAAGAGAGGGCCCGTCTGGAACAAGAGATGGAGACGCTGACCGCGCACCGGGCGCAGGCGGAAGAGAACCTCGGCGACAGCGACCGGTATGACCAGCGCCg                                <  2:8396199/140‑1 (MQ=255)
                                                                                                       cGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCg                                <  1:7740465/140‑1 (MQ=255)
                                                                                                            agctgagctgCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAg                           <  2:3735709/140‑1 (MQ=255)
                                                                                                            agctgagctgCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAg                           <  2:9611655/140‑1 (MQ=255)
                                                                                                             tctgagctgCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGc                          <  2:6961149/139‑1 (MQ=255)
                                                                                                             gctgagcggCGTGCGCAAACCCAGCCACTGCGTAAAGCGATTGCCCGTCTGGAAACAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGGGTACTGTATGACCAGAGCCGTAAAGc                          <  2:6638021/140‑1 (MQ=255)
                                                                                                                  gctgCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGt                     >  1:4281976/1‑140 (MQ=255)
                                                                                                                        tgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCg               >  1:8956118/1‑140 (MQ=255)
                                                                                                                        tgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCg               >  1:8573062/1‑140 (MQ=255)
                                                                                                                        tgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCg               >  2:6415711/1‑140 (MQ=255)
                                                                                                                         gcgcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc              <  1:10179097/140‑1 (MQ=255)
                                                                                                                         gcgcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc              <  2:4900717/140‑1 (MQ=255)
                                                                                                                         gcgcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc              <  1:5317213/140‑1 (MQ=255)
                                                                                                                         gcgcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc              <  1:4946298/140‑1 (MQ=255)
                                                                                                                             aaaCCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgcctgc          >  1:483412/1‑140 (MQ=255)
                                                                                                                                cccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGcctgcctg       >  1:1402380/1‑140 (MQ=255)
                                                                                                                                cccAGCCACTGCGTAAAGAGATTGCCCGCCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGcctgcctg       >  1:6863695/1‑140 (MQ=255)
                                                                                                                                 ccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc      <  2:5838121/140‑1 (MQ=255)
                                                                                                                                 ccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc      <  1:9149793/140‑1 (MQ=255)
                                                                                                                                 ccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc      <  1:10491239/140‑1 (MQ=255)
                                                                                                                                 ccAGCCACTGCGTAAAGAGAGTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGACCGCGCACCTGGCGCAGGCGGAAGAGAACCGCGGCGACAGCGAACTGTATGACCAGAGCCGTAACGCGGAGTTGACCGCctgcctgc      <  2:2732498/140‑1 (MQ=255)
                                                                                                                                    gCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAAc   >  1:7964351/1‑140 (MQ=255)
                                                                                                                                     ccACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAACa  >  2:9727161/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TTATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAACA  >  NZ_CP025268/3483516‑3483788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑