Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,544,760 T→C *257* (TAA→TAG bioH ← pimeloyl‑ACP methyl ester esterase BioH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,544,7600TC99.8% 1759.3 / NA 503*257* (TAA→TAGbioHpimeloyl‑ACP methyl ester esterase BioH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (294/208);  minor base G (0/1);  total (294/209)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 503 total aligned reads displayed.

CAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTG  >  NZ_CP025268/3544623‑3544895
                                                                                                                                         |                                                                                                                                       
  aaGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTaca                                                                                                                                     <  1:132240/140‑1 (MQ=255)
   aGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTacac                                                                                                                                    >  2:7260556/1‑140 (MQ=255)
     cccTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACAccc                                                                                                                                  <  1:8826973/140‑1 (MQ=255)
     cccTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACAccc                                                                                                                                  >  1:2271309/1‑140 (MQ=255)
       cTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTAACGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCctc                                                                                                                                >  2:9090706/1‑140 (MQ=255)
        tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCctct                                                                                                                               >  1:6542552/1‑140 (MQ=255)
        tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCctct                                                                                                                               >  2:624144/1‑140 (MQ=255)
          cATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGc                                                                                                                             >  2:2518694/1‑140 (MQ=255)
               aGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTcta                                                                                                                        >  2:4658004/1‑138 (MQ=255)
               aGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCaa                                                                                                                        >  1:6865596/1‑140 (MQ=255)
                  cacaataagGCTTGAATCCGGAGTTCCGCTTGCAGCCATCATGCGCTTTACCGGGCTGGCAGAAGAAGAGGTGGCTGCGGCGAGTCAGGACAGTTCTGTCTCGCCCTTTCAAAGGCCACCTACCCCCTCGGCGTCAACGc                                                                                                                     >  2:5188718/4‑140 (MQ=255)
                   aaaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTTCACCCTCTGCTTCAACGcc                                                                                                                    <  2:5736636/140‑1 (MQ=255)
                    aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCctct                                                                                                                               <  1:7873189/128‑1 (MQ=255)
                    aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGcca                                                                                                                   >  1:6592956/1‑140 (MQ=255)
                    aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGcca                                                                                                                   >  1:6694027/1‑140 (MQ=255)
                    aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGcca                                                                                                                   >  1:4484024/1‑140 (MQ=255)
                    aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGcca                                                                                                                   >  1:2622532/1‑140 (MQ=255)
                        aatGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACcag                                                                                                               <  2:4939589/140‑1 (MQ=255)
                          tGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTCAAGTTCTGTCTCGCCAGTTCAAACGCCCCCTACACCCTCTGCTTCATCGCCACcagcc                                                                                                             >  2:8170883/1‑139 (MQ=255)
                           gCTTGAATCCGGAGTTCCTCGTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACcagcag                                                                                                            >  2:9273664/1‑140 (MQ=255)
                            cTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAgg                                                                                                           <  1:10592313/140‑1 (MQ=255)
                                 aTCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACa                                                                                                      >  2:1118537/1‑140 (MQ=255)
                                            ctctTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCg                                                                                           >  1:4637921/1‑140 (MQ=255)
                                              ctTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGa                                                                                         >  1:8784742/1‑140 (MQ=255)
                                               ttGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                                        <  1:8424676/140‑1 (MQ=255)
                                                 gCAGACATCATGCGCTTTACCGGGCTGTCAGCAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGc                                                                                      <  1:7766492/140‑1 (MQ=255)
                                                 gCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACg                                                                                                                      <  1:8753707/108‑1 (MQ=255)
                                                 gCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGc                                                                                      <  2:568516/140‑1 (MQ=255)
                                                     aCATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaa                                                                                  >  1:9547169/1‑140 (MQ=255)
                                                     aCATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaa                                                                                  >  1:9141570/1‑140 (MQ=255)
                                                      catcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCAGTTCAAACGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCAGCCAGCGaaat                                                                                 >  2:7048619/1‑140 (MQ=255)
                                                       atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaata                                                                                >  2:884171/1‑140 (MQ=255)
                                                       atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaata                                                                                >  2:164233/1‑140 (MQ=255)
                                                       atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaata                                                                                >  2:1191788/1‑140 (MQ=255)
                                                       atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaata                                                                                >  1:10720752/1‑140 (MQ=255)
                                                       atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGACGGATGCGaaata                                                                                >  2:10306225/1‑140 (MQ=255)
                                                         catGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataaa                                                                              >  2:3942644/1‑140 (MQ=255)
                                                         catGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataaa                                                                              >  1:8594587/1‑140 (MQ=255)
                                                         catGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataaa                                                                              >  1:3206050/1‑140 (MQ=255)
                                                           tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATg                                                                            >  2:3449513/1‑140 (MQ=255)
                                                           tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATg                                                                            >  2:8706268/1‑140 (MQ=255)
                                                           tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATg                                                                            >  2:3871277/1‑140 (MQ=255)
                                                           tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATg                                                                            >  1:5370698/1‑140 (MQ=255)
                                                                tttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCa                                                                       <  1:3793244/140‑1 (MQ=255)
                                                                 ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCAt                                                                      >  2:596111/1‑140 (MQ=255)
                                                                 ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCAt                                                                      >  1:3522584/1‑140 (MQ=255)
                                                                 ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCAt                                                                      >  2:6562379/1‑140 (MQ=255)
                                                                  tACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATg                                                                     >  2:4299921/1‑140 (MQ=255)
                                                                   aCCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATgg                                                                    >  1:8290064/1‑140 (MQ=255)
                                                                   aCCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATgg                                                                    >  2:1947058/1‑140 (MQ=255)
                                                                   aCCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAGGCGAACTACATGGCGCATgg                                                                    >  2:2774112/1‑140 (MQ=255)
                                                                    ccGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATTAATGGCGCATggg                                                                   <  1:5763704/140‑1 (MQ=255)
                                                                     cGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGc                                                                  <  1:9766354/140‑1 (MQ=255)
                                                                       ggCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCg                                                                >  1:2027156/1‑140 (MQ=255)
                                                                       ggCTGTCAGAAGAAGAGTTGGCGGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGACCTAAATGGCGCATGGGCCg                                                                >  2:2927844/1‑140 (MQ=255)
                                                                         cTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCGCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCt                                                              >  2:359435/1‑140 (MQ=255)
                                                                          tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCtt                                                             >  1:4943523/1‑140 (MQ=255)
                                                                          tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCtt                                                             >  2:5309501/1‑140 (MQ=255)
                                                                          tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCtt                                                             >  1:1599330/1‑140 (MQ=255)
                                                                          tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCtt                                                             >  2:540789/1‑140 (MQ=255)
                                                                          tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTATCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCtt                                                             >  2:10042225/1‑140 (MQ=255)
                                                                            tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTg                                                           >  2:2356886/1‑140 (MQ=255)
                                                                            tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTg                                                           >  2:3129061/1‑140 (MQ=255)
                                                                            tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTg                                                           >  1:5014178/1‑140 (MQ=255)
                                                                            tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTg                                                           >  1:7103407/1‑140 (MQ=255)
                                                                              agaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGc                                                         <  2:9780909/140‑1 (MQ=255)
                                                                              agaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGc                                                         <  1:1869468/140‑1 (MQ=255)
                                                                              agaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGc                                                         <  1:2932352/140‑1 (MQ=255)
                                                                               gaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                        <  2:33095/140‑1 (MQ=255)
                                                                               gaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                        <  1:2417697/140‑1 (MQ=255)
                                                                               gaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                        <  2:8023906/140‑1 (MQ=255)
                                                                               gaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                        <  2:6815991/140‑1 (MQ=255)
                                                                                aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                                       <  1:3409684/140‑1 (MQ=255)
                                                                                  gaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAg                                                     <  2:9725295/140‑1 (MQ=255)
                                                                                  gaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAg                                                     >  1:1158585/1‑140 (MQ=255)
                                                                                  gaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAg                                                     <  2:5032841/140‑1 (MQ=255)
                                                                                      agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGatat                                                 >  1:7426199/1‑140 (MQ=255)
                                                                                      agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGatat                                                 >  2:8399387/1‑140 (MQ=255)
                                                                                      agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGatat                                                 >  1:5460142/1‑140 (MQ=255)
                                                                                            cTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGAtt                                           <  2:7527711/140‑1 (MQ=255)
                                                                                             tGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCACTGCTTCAACGCCACCAGCAGGTGACAAAAATCGGCCGTATGCGAAATAAATGGCGCATGGGCCCCTTTGGCGAAGATATATGATTc                                          >  2:1812071/1‑140 (MQ=255)
                                                                                                  cgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCtgt                                     <  2:10180645/140‑1 (MQ=255)
                                                                                                        aGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                               <  2:8605159/140‑1 (MQ=255)
                                                                                                        aGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                               <  1:9410857/140‑1 (MQ=255)
                                                                                                        aGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                               <  1:9048317/140‑1 (MQ=255)
                                                                                                        aGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                               <  2:1224541/140‑1 (MQ=255)
                                                                                                                   gTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATcc                    <  2:9003922/140‑1 (MQ=255)
                                                                                                                   gTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATcc                    <  1:5906838/140‑1 (MQ=255)
                                                                                                                    tctcGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCa                   >  1:6462338/1‑140 (MQ=255)
                                                                                                                        gCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCATGTGACAAAACTCGGCCGGATGCGAACTAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGTCCAAAGTTTATCCAGCAt               >  2:3743471/1‑140 (MQ=255)
                                                                                                                         ccATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATc              >  1:621160/1‑140 (MQ=255)
                                                                                                                         ccATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATc              >  1:4096493/1‑140 (MQ=255)
                                                                                                                         ccATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATc              >  2:9606465/1‑140 (MQ=255)
                                                                                                                         ccATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATc              >  1:10000951/1‑140 (MQ=255)
                                                                                                                              tCAAAAGCCACCTACACCCTCTGCTTCAGCGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGcac         >  1:2775440/1‑140 (MQ=255)
                                                                                                                              tCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGcac         >  1:3896188/1‑140 (MQ=255)
                                                                                                                                  aaGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACt     <  2:7747093/140‑1 (MQ=255)
                                                                                                                                    gCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACttt   >  1:8160936/1‑140 (MQ=255)
                                                                                                                                    gCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACttt   >  2:9528572/1‑140 (MQ=255)
                                                                                                                                     ccACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTg  >  1:9359130/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTG  >  NZ_CP025268/3544623‑3544895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: