BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,567,938	T→C	L261L (TTA→TTG)	glgA ←	glycogen synthase GlgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,567,938	0	T	C	99.8%	2012.3 / NA	577	L261L (TTA→TTG)	glgA	glycogen synthase GlgA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (257/319); minor base A (0/1); total (257/320)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 577 total aligned reads displayed.

AGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGAG  >  NZ_CP025268/3567801‑3568074

agaCCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCaa                                                                                                                                        >  1:8841285/1‑140 (MQ=255)

agaCCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCaa                                                                                                                                        >  1:7720778/1‑140 (MQ=255)

 gaCCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAAc                                                                                                                                       >  2:4448912/1‑140 (MQ=255)

         ttgctggTCAGACGGCTCACCACTTCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                               <  2:6737142/138‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                               <  2:4716616/140‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                               <  1:6276981/140‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                               <  2:1034787/140‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                               <  2:10069460/140‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                               <  2:5587239/140‑1 (MQ=255)

           gctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCg                                                                                                                             >  2:6995577/1‑140 (MQ=255)

           gctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCg                                                                                                                             >  2:8014506/1‑140 (MQ=255)

           gctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCg                                                                                                                             >  2:9244836/1‑140 (MQ=255)

            ctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGt                                                                                                                            <  2:4957413/140‑1 (MQ=255)

               gTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGtctc                                                                                                                         >  1:4139850/1‑140 (MQ=255)

               gTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGtctc                                                                                                                         >  1:7887532/1‑140 (MQ=255)

                     cGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGAGTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGGATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACt                                                                                                                   >  2:10202918/1‑140 (MQ=255)

                         tCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAg                                                                                                               >  1:10133074/1‑140 (MQ=255)

                         tCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAg                                                                                                               >  2:2264441/1‑140 (MQ=255)

                          caccacTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGa                                                                                                              >  2:7519021/1‑140 (MQ=255)

                          caccacTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGa                                                                                                              >  2:9741929/1‑140 (MQ=255)

                            ccacTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGAtt                                                                                                            >  1:10776669/1‑140 (MQ=255)

                              acTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGAtttt                                                                                                          >  2:5722976/1‑140 (MQ=255)

                              acTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGAtttt                                                                                                          <  2:9521640/140‑1 (MQ=255)

                              acTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGAtttt                                                                                                          <  2:6115971/140‑1 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                      >  1:7466861/1‑140 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                      >  1:6573349/1‑140 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                      >  2:3971738/1‑140 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                      >  1:2330412/1‑140 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATGTGTAACTGGCGCTTATATTCCGCTTTATCTTCCAACGTATCTCGGGTGAAACGCGAGTCCAACAGAAAGTCCGTCTCTGGACTCCAGATTTTATCg                                                                                                      >  1:6893889/1‑140 (MQ=255)

                                   aaaaaGCGGCACTTTATCGTCATCCTTAAGACCCAGTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACTTATCGCGGGTGTAACGCGATGACAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGt                                                                                                     <  2:1097782/140‑1 (MQ=255)

                                   aaaaaGCGGCACTTTATCGTCAACCTTAAGCCCCTTTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACCGTACGTCCGTCTCTTGACTCCAGATTTTCTCGt                                                                                                     <  1:7024747/140‑1 (MQ=255)

                                   aaaaaGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTACGTCCGTCTCTGGACTCCAGATTTTCTCGt                                                                                                     <  2:10448357/140‑1 (MQ=255)

                                   aaaaaGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGt                                                                                                     <  1:1562589/140‑1 (MQ=255)

                                     aaaGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTcc                                                                                                   <  1:8284322/140‑1 (MQ=255)

                                      aaGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTTTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCa                                                                                                  <  2:4155393/140‑1 (MQ=255)

                                       aGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCAc                                                                                                 >  1:6360627/1‑140 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                                <  2:5098550/140‑1 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                                <  2:5476944/140‑1 (MQ=255)

                                          ggCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGcc                                                                                              <  1:1803819/140‑1 (MQ=255)

                                                ttATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAg                                                                                        <  2:9063407/140‑1 (MQ=255)

                                                  aTCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTa                                                                                      <  1:9144891/140‑1 (MQ=255)

                                                         aCCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGa                                                                               >  2:1765998/1‑140 (MQ=255)

                                                         aCCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGa                                                                               >  2:2117389/1‑140 (MQ=255)

                                                         aCCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGa                                                                               >  1:4338443/1‑140 (MQ=255)

                                                             tAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGa                                                                           >  1:206954/1‑140 (MQ=255)

                                                              aaGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGAc                                                                          <  1:8073930/140‑1 (MQ=255)

                                                              aaGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGAc                                                                          <  1:8237287/140‑1 (MQ=255)

                                                               aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACg                                                                         <  1:5980715/140‑1 (MQ=255)

                                                               aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACg                                                                         <  1:3208295/140‑1 (MQ=255)

                                                                    cATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCtt                                                                    <  1:3859245/140‑1 (MQ=255)

                                                                    cATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCtt                                                                    <  1:1175981/140‑1 (MQ=255)

                                                                    cATTGCGATTTGTAACTCGCGCTTATCTTCCGCATTATCTTCCAACCTACCGCGGGTGTAACGCGAGGCCAACAGCAAGTCCCTCTCTGGACTCCAGAGTTTCTCCTCCACGCCGTTCAGTACGCCGGAAAGACGCCCtt                                                                    <  2:3035735/140‑1 (MQ=255)

                                                                      ttGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGCCTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCa                                                                  >  1:2999440/1‑140 (MQ=255)

                                                                      ttGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCa                                                                  <  1:1335716/140‑1 (MQ=255)

                                                                      ttGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCa                                                                  <  2:3187122/140‑1 (MQ=255)

                                                                      ttGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCa                                                                  >  2:2679564/1‑140 (MQ=255)

                                                                         cGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACgg                                                               <  2:1067731/140‑1 (MQ=255)

                                                                          gATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGt                                                              <  1:10385826/140‑1 (MQ=255)

                                                                             ttGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                           <  1:6436515/140‑1 (MQ=255)

                                                                             ttGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                           <  2:7169921/140‑1 (MQ=255)

                                                                             ttGTAACTGGCGCGTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                           <  2:3758159/140‑1 (MQ=255)

                                                                               gTAACTGGCGCTTATTTTCCGCTTTATCTTCCTACGTATCGCGGGTGTAACGGGAGGCCAACAGTACGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCAGTTCACGGTGACGc                                                         <  2:8775198/140‑1 (MQ=255)

                                                                               gTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGc                                                         <  2:10537134/140‑1 (MQ=255)

                                                                               gTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGc                                                         <  1:2128175/140‑1 (MQ=255)

                                                                                 aaCTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTg                                                       <  1:4561323/140‑1 (MQ=255)

                                                                                      gcgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTg                                                                                                                       >  1:9981774/1‑71 (MQ=255)

                                                                                      gcgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCAGTTGCa                                                  >  1:6760186/1‑140 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                              <  1:4917081/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                              <  2:5258511/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                              <  1:3441064/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                              <  1:5061834/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                              <  1:6060417/140‑1 (MQ=255)

                                                                                            aTTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGAc                                            <  1:8939489/140‑1 (MQ=255)

                                                                                             ttttCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGAcc                                           <  1:9244837/140‑1 (MQ=255)

                                                                                                tCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTc                                        >  2:4329299/1‑140 (MQ=255)

                                                                                                 ccGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTcc                                       <  2:3405880/140‑1 (MQ=255)

                                                                                                 ccGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGGAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTcc                                       <  1:5897385/140‑1 (MQ=255)

                                                                                                 ccGCTTTATCGTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTcc                                       <  2:6343597/140‑1 (MQ=255)

                                                                                                            tCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTACGTCTCTGGACACCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGc                            >  1:5173242/1‑140 (MQ=255)

                                                                                                              cAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCaa                          >  1:2804711/1‑140 (MQ=255)

                                                                                                              cAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGGCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTCGGCaa                          >  2:1405972/1‑140 (MQ=255)

                                                                                                                  gTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTg                      <  2:7882189/140‑1 (MQ=255)

                                                                                                                   tATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGc                     >  1:9469031/1‑140 (MQ=255)

                                                                                                                   tATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGc                     >  2:7342023/1‑140 (MQ=255)

                                                                                                                       gcgGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGtt                 <  1:9787316/140‑1 (MQ=255)

                                                                                                                       gcgGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGtt                 <  1:5470637/140‑1 (MQ=255)

                                                                                                                         gggTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCg               <  1:3678165/140‑1 (MQ=255)

                                                                                                                            tgtAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCa                                                                                                  >  1:2489186/1‑54 (MQ=255)

                                                                                                                            tgtAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCa                                                                                                  <  2:2489186/54‑1 (MQ=255)

                                                                                                                              tAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGAt          >  1:8481084/1‑140 (MQ=255)

                                                                                                                                  gcgAGGCCAACAGTACGTCCGACTCTGTACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcg      <  2:9367866/140‑1 (MQ=255)

                                                                                                                                  gcgAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcg      <  1:7096082/140‑1 (MQ=255)

                                                                                                                                  gcgAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcg      <  1:7213799/140‑1 (MQ=255)

                                                                                                                                  gcgAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcg      <  1:3368787/140‑1 (MQ=255)

                                                                                                                                   cgAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgc     <  2:4553977/140‑1 (MQ=255)

                                                                                                                                   cgAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgc     <  2:5614603/140‑1 (MQ=255)

                                                                                                                                     aGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGa   >  2:8192805/1‑140 (MQ=255)

                                                                                                                                     aGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGa   >  1:8565586/1‑140 (MQ=255)

                                                                                                                                     aGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGa   >  1:10202713/1‑140 (MQ=255)

                                                                                                                                      ggCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGAg  >  1:5713950/1‑140 (MQ=255)

AGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGAG  >  NZ_CP025268/3567801‑3568074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`