BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,743,921	T→C	I172I (ATT→ATC)	galK →	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,743,921	0	T	C	99.8%	1912.9 / NA	548	I172I (ATT→ATC)	galK	galactokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (254/293); minor base A (1/0); total (255/293)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 548 total aligned reads displayed.

TAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743785‑3744056

tAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATg                                                                                                                                      >  1:3047113/1‑140 (MQ=255)

tAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATg                                                                                                                                      >  2:1433148/1‑140 (MQ=255)

tAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATg                                                                                                                                      >  1:2787680/1‑140 (MQ=255)

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  1:4437969/1‑140 (MQ=255)

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTGTGTAGGCTGTAACGGCGGGAGCATGGa                                                                                                                                    >  2:6733515/1‑140 (MQ=255)

   gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  1:6989905/140‑1 (MQ=255)

   gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  2:10478187/140‑1 (MQ=255)

   gTTCGTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  1:10859099/140‑1 (MQ=255)

        tcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTGATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTATGTAGGCTGTAACTGCGGGAGCATGGATCAgat                                                                                                                              >  2:8051350/1‑138 (MQ=255)

            cTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTGTATCATCTGGCGCTGGACGGCGCACAAATCGCGCTTAACGGGCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCTTGGATCAGCTAAtt                                                                                                                          >  2:502709/1‑140 (MQ=255)

                 cTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCcgc                                                                                                                     >  1:10383899/1‑140 (MQ=255)

                  tGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCcgcg                                                                                                                    <  2:224878/140‑1 (MQ=255)

                  tGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCcgcg                                                                                                                    <  2:5890056/140‑1 (MQ=255)

                    gAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCt                                                                                                                  <  1:3285034/140‑1 (MQ=255)

                     aaGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTc                                                                                                                 <  1:8948065/140‑1 (MQ=255)

                       gTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCgg                                                                                                               >  2:235410/1‑140 (MQ=255)

                       gTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGATAATTTCCGCGCTCgg                                                                                                               >  2:8171484/1‑140 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  1:2598776/1‑140 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  1:475203/1‑140 (MQ=255)

                          gcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACCAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTACCTGCGGGCTCATGGATCAGCTAAGTTCCGCGCTCGGccc                                                                                                            >  2:7780502/1‑138 (MQ=255)

                           cgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaag                                                                                                           <  2:4928686/140‑1 (MQ=255)

                           cgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaag                                                                                                           <  1:6096674/140‑1 (MQ=255)

                            ggTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaaga                                                                                                          <  1:8496364/140‑1 (MQ=255)

                                     cGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATg                                                                                                 <  2:1937639/140‑1 (MQ=255)

                                       tATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGcc                                                                                               <  2:5153722/140‑1 (MQ=255)

                                       tATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGcc                                                                                               >  1:3937443/1‑140 (MQ=255)

                                       tATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGcc                                                                                               >  2:326400/1‑140 (MQ=255)

                                       tATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGcc                                                                                               >  1:4559250/1‑140 (MQ=255)

                                        aTTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCt                                                                                              <  2:900424/140‑1 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCCGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAGCAGCGAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  2:5705870/1‑140 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  2:4410817/1‑140 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  2:6421654/1‑140 (MQ=255)

                                              gcagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGa                                                                                        >  1:8303394/1‑140 (MQ=255)

                                               cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       <  2:3893985/140‑1 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  2:1531143/1‑140 (MQ=255)

                                                        catcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCg                                                                              <  1:6549738/140‑1 (MQ=255)

                                                         atcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGc                                                                             <  1:5700461/140‑1 (MQ=255)

                                                         atcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGc                                                                             >  1:9152953/1‑140 (MQ=255)

                                                          tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  1:5498526/140‑1 (MQ=255)

                                                          tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  1:9838749/140‑1 (MQ=255)

                                                          tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  1:8162704/140‑1 (MQ=255)

                                                             gCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCAc                                                                         >  1:10024819/1‑140 (MQ=255)

                                                                    gACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGAc                                                                  <  1:5205129/140‑1 (MQ=255)

                                                                    gACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGAc                                                                  <  2:8854607/140‑1 (MQ=255)

                                                                      cGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCa                                                                <  1:7476831/140‑1 (MQ=255)

                                                                       ggCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCaa                                                               <  2:6549794/140‑1 (MQ=255)

                                                                        gcgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCaaa                                                              >  2:4192954/1‑140 (MQ=255)

                                                                        gcgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCaaa                                                              >  2:1289042/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:7969512/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:7199414/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  2:618606/1‑140 (MQ=255)

                                                                           cacaAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCa                                                           <  1:1475707/140‑1 (MQ=255)

                                                                            acaAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAg                                                          <  2:4043350/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGTAAGATGAAAACCAGTTTGTAGGCTGTAACTGCGTGATCATTGATCAGCTAATTTCCGCGATATGCAAGAAATTTAATGCCTTGCTGATCGATTTCCGCTCACTGGGGACCAAATCAGTTTcc                                                     <  2:1833370/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:1724781/140‑1 (MQ=255)

                                                                                     gcTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGc                                                 <  2:853776/140‑1 (MQ=255)

                                                                                        tAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCa                                              >  1:8395189/1‑140 (MQ=255)

                                                                                        tAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCa                                              >  1:5105720/1‑140 (MQ=255)

                                                                                         aaCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCTCTCGGAAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCaa                                             <  2:9447357/140‑1 (MQ=255)

                                                                                         aaCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCaa                                             <  1:6301465/140‑1 (MQ=255)

                                                                                            ggTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAgg                                          <  2:544100/140‑1 (MQ=255)

                                                                                                 ggAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTgg                                     <  2:10123634/140‑1 (MQ=255)

                                                                                                   aaGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCt                                   <  2:5774419/140‑1 (MQ=255)

                                                                                                     gCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgt                                 >  2:8364137/1‑140 (MQ=255)

                                                                                                     gCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgt                                 >  1:3237248/1‑140 (MQ=255)

                                                                                                      cAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtc                                >  2:6961582/1‑140 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:1895872/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:9284087/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:4835504/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:3776840/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:4900128/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:1315056/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:6693743/140‑1 (MQ=255)

                                                                                                        gAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgt                              >  2:7952078/1‑140 (MQ=255)

                                                                                                         aacaCCAGATTGTAGGCGGGACCTGCGCGATCCAGGATCACCAACGTACCGCGCGCGGCAAGAAAGATCAGCCCGGGCCGATCGCTTGCCGCTCCCGGGGGACCAAAGCAGTTTCCATGCCCCCAGGTGTGGCTgtcgtc                             <  2:7396679/137‑1 (MQ=255)

                                                                                                            aCCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTg                                  <  2:7243947/132‑1 (MQ=255)

                                                                                                            aCCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGtcatc                          <  1:6439238/140‑1 (MQ=255)

                                                                                                               aGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGtcatcatc                       <  2:306063/140‑1 (MQ=255)

                                                                                                                gTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgtc                             >  1:2121757/1‑133 (MQ=255)

                                                                                                                   tGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCACGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACa                   >  2:7860789/1‑140 (MQ=255)

                                                                                                                   tGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACa                   <  1:4192780/140‑1 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  1:4107769/140‑1 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  1:2443700/140‑1 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 >  1:3525696/1‑140 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  1:7811991/140‑1 (MQ=255)

                                                                                                                        gCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAAc              <  1:5418352/140‑1 (MQ=255)

                                                                                                                        gCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAAc              >  1:5906844/1‑140 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            <  2:913564/140‑1 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            <  1:7663043/140‑1 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCGCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            <  2:2549564/140‑1 (MQ=255)

                                                                                                                           gTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTc           >  1:10579263/1‑140 (MQ=255)

                                                                                                                           gTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTc           >  2:10668412/1‑140 (MQ=255)

                                                                                                                            tAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCa          >  2:473165/1‑140 (MQ=255)

                                                                                                                            tAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCa          >  1:10490427/1‑140 (MQ=255)

                                                                                                                             aaCTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCaa         <  1:9594828/140‑1 (MQ=255)

                                                                                                                                tGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACg      <  1:10144047/140‑1 (MQ=255)

                                                                                                                                  cGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTa    <  2:9091278/140‑1 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  <  2:3388891/140‑1 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  >  2:9735190/1‑140 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATGTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGGAcc  <  2:6083819/140‑1 (MQ=255)

TAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743785‑3744056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`