BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,768,238	A→G	1378 (TAA→TAG)	rhsA →	RHS element protein RhsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,768,238	0	A	G	99.9%	4496.1 / NA	1285	1378 (TAA→TAG)	rhsA	RHS element protein RhsA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (255/1029); minor base C (0/1); total (255/1030)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1285 total aligned reads displayed.

GATGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTTGATGATC  >  NZ_CP025268/3768101‑3768361

gATGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGcc                                                                                                                           >  1:925571/1‑140 (MQ=255)

gATGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGcc                                                                                                                           >  1:7251310/1‑140 (MQ=255)

 aTGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGccc                                                                                                                          <  2:5216167/140‑1 (MQ=255)

 aTGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGccc                                                                                                                          <  2:953029/140‑1 (MQ=255)

 aTGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGccc                                                                                                                          <  2:7964825/140‑1 (MQ=255)

 aTGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGccc                                                                                                                          >  2:2336791/1‑140 (MQ=255)

 aTGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGcc                                                                                                                           >  1:1100865/1‑139 (MQ=255)

   gggAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCtt                                                                                                                        <  1:5137544/140‑1 (MQ=255)

    ggAAAAAACCCGTCAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTtg                                                                                                                       >  1:8584931/1‑140 (MQ=255)

       aaaaaCCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATTCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTgg                                                                                                                    <  1:7446899/140‑1 (MQ=255)

        aaaaCCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGa                                                                                                                   <  1:1459624/140‑1 (MQ=255)

        aaaaCCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGa                                                                                                                   <  1:4887237/140‑1 (MQ=255)

                aCAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTg                                                                                                           <  2:133018/140‑1 (MQ=255)

                  aGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAACGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCa                                                                                                         <  1:977945/140‑1 (MQ=255)

                  aGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCa                                                                                                         <  1:2934995/140‑1 (MQ=255)

                  aGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCa                                                                                                         <  2:4017012/140‑1 (MQ=255)

                  aGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCa                                                                                                         <  1:363774/140‑1 (MQ=255)

                       tgACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTc                                                                                                    <  2:7036147/140‑1 (MQ=255)

                       tgACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTc                                                                                                    <  2:868698/140‑1 (MQ=255)

                         aCGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCaa                                                                                                  <  1:4359590/140‑1 (MQ=255)

                          cGTACTTCAGGAACTAATTGATTGTGNTGATATTTGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTCNCTGCAATGTCaaa                                                                                                 <  1:5748036/140‑1 (MQ=255)

                            tACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAata                                                                                               <  2:6924548/140‑1 (MQ=255)

                            tACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAata                                                                                               <  2:8282802/140‑1 (MQ=255)

                            tACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAata                                                                                               <  2:4720809/140‑1 (MQ=255)

                            tACTTCAGGAACTAATTGATTGTGGTGAGATTAGTGCTAAAGATGCAACAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAata                                                                                               <  2:7217079/140‑1 (MQ=255)

                              cTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAataca                                                                                             <  1:10012483/140‑1 (MQ=255)

                                 cAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTtgtg                                                                                                                     >  1:7112002/1‑113 (MQ=255)

                                 cAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAatacatac                                                                                          >  2:7415971/1‑140 (MQ=255)

                                   ggAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCa                                                                                        <  1:4568664/140‑1 (MQ=255)

                                   ggAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCa                                                                                        <  2:9772524/140‑1 (MQ=255)

                                    gAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCag                                                                                       <  2:5346631/140‑1 (MQ=255)

                                     aaCTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCaga                                                                                      <  1:3901938/140‑1 (MQ=255)

                                      aCTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaa                                                                                     >  2:6717383/1‑140 (MQ=255)

                                       cTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaa                                                                                    <  2:10588404/140‑1 (MQ=255)

                                         aaTTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGGATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGa                                                                                                                   <  1:6357660/107‑1 (MQ=255)

                                         aaTTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGccc                                                                                                                          <  1:9424711/100‑1 (MQ=255)

                                         aaTTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaag                                                                                  <  2:4925006/140‑1 (MQ=255)

                                         aaTTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaag                                                                                  >  1:88679/1‑140 (MQ=255)

                                            tgattgTGGTGATATTAGTTCTAAAGATGAAAAAAGCACACAGATAGCCTTGAATTGTCGTCACTACTGAAAGTCAAACTATAAATAAAGATAGCCCTTGTGGAGGTTCCTGCTATGTAATTTACATATCagaaaagaaa                                                                               <  2:306103/140‑1 (MQ=255)

                                            tgattgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaagaaa                                                                               <  1:6898021/140‑1 (MQ=255)

                                            tgattgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaagaaa                                                                               <  1:8084516/140‑1 (MQ=255)

                                              attgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAgg                                                                             <  2:5457920/140‑1 (MQ=255)

                                              attgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAgg                                                                             <  1:9021312/140‑1 (MQ=255)

                                               ttgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                                                                            <  2:10853041/140‑1 (MQ=255)

                                               ttgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                                                                            <  1:7821523/140‑1 (MQ=255)

                                               ttgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                                                                            <  2:9077423/140‑1 (MQ=255)

                                                tgtgGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCa                                                                           <  1:10235452/140‑1 (MQ=255)

                                                  tggtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAg                                                                         >  2:4486126/1‑140 (MQ=255)

                                                   ggtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGt                                                                        <  2:1973566/140‑1 (MQ=255)

                                                    gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       <  1:1732845/140‑1 (MQ=255)

                                                    gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       <  2:3606086/140‑1 (MQ=255)

                                                    gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       <  1:6007107/140‑1 (MQ=255)

                                                    gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       <  2:6230161/140‑1 (MQ=255)

                                                    gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       <  1:2875287/140‑1 (MQ=255)

                                                    gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       <  2:1689072/140‑1 (MQ=255)

                                                    gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       <  2:8825192/140‑1 (MQ=255)

                                                    gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       <  2:8453364/140‑1 (MQ=255)

                                                     tgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaa                                                                      <  1:6734595/140‑1 (MQ=255)

                                                     tgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaa                                                                      <  2:7684648/140‑1 (MQ=255)

                                                      gATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                     <  1:2530096/140‑1 (MQ=255)

                                                      gATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                     <  2:5978217/140‑1 (MQ=255)

                                                      gATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                     <  1:3091247/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:270344/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:2240688/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:1627321/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:6360764/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:7838395/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:4046027/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:9639525/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:5578657/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:4303073/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:3783559/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:10407020/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:10651045/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  1:7641476/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:2598642/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:2818056/140‑1 (MQ=255)

                                                          ttAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:3236010/140‑1 (MQ=255)

                                                           tAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTa                                                                <  1:3297381/140‑1 (MQ=255)

                                                            aGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:178986/138‑1 (MQ=255)

                                                            aGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTAt                                                               <  1:2093777/140‑1 (MQ=255)

                                                              tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATgg                                                             <  2:2921957/140‑1 (MQ=255)

                                                              tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATgg                                                             <  1:3437981/140‑1 (MQ=255)

                                                              tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATgg                                                             <  2:1036391/140‑1 (MQ=255)

                                                               gCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                       >  2:1240805/1‑129 (MQ=255)

                                                               gCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGt                                                            <  2:8183833/140‑1 (MQ=255)

                                                                  aaaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTta                                                         <  2:5649739/140‑1 (MQ=255)

                                                                   aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaagaaa                                                                               >  2:2136411/1‑117 (MQ=255)

                                                                   aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:3368514/131‑1 (MQ=255)

                                                                   aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtat                                                        <  2:1560215/140‑1 (MQ=255)

                                                                        gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTgg                                                                                                                    <  1:1266554/75‑1 (MQ=255)

                                                                          aaaaaGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCa                                                 <  1:6224994/140‑1 (MQ=255)

                                                                                cacacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTaa                                           <  1:5386435/140‑1 (MQ=255)

                                                                                               gAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                                                                            <  2:10167596/92‑1 (MQ=255)

                                                                                                 aTTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAatgatgatg                          <  1:9903329/140‑1 (MQ=255)

                                                                                                     tcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGa                                                                                                                   <  1:8251484/47‑1 (MQ=255)

                                                                                                            tCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 <  2:6588723/90‑1 (MQ=255)

                                                                                                            tCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                 >  1:6588724/1‑90 (MQ=255)

                                                                                                                   aGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                     <  2:9387099/79‑1 (MQ=255)

                                                                                                                     tCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                     >  1:1904000/1‑77 (MQ=255)

GATGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTTGATGATC  >  NZ_CP025268/3768101‑3768361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`