Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,884,842 T→C D269G (GAT→GGT)  dnaA ← chromosomal replication initiator protein DnaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,884,8420TC99.8% 2182.7 / NA 626D269G (GAT→GGT) dnaAchromosomal replication initiator protein DnaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (239/386);  minor base A (1/0);  total (240/386)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 633 total aligned reads displayed.

TGTCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGATCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGCG  >  NZ_CP025268/3884705‑3884978
                                                                                                                                         |                                                                                                                                        
tGTCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGAccc                                                                                                                                        <  1:2181079/140‑1 (MQ=255)
    gTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAgg                                                                                                                                    <  1:6446385/140‑1 (MQ=255)
    gTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAgg                                                                                                                                    <  1:3720846/140‑1 (MQ=255)
    gTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAgg                                                                                                                                    <  2:876437/140‑1 (MQ=255)
     ttttCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGt                                                                                                                                   >  1:1340669/1‑140 (MQ=255)
            cgGACTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTTGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAAAGATCCTCAACTCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATg                                                                                                                            <  2:9042013/140‑1 (MQ=255)
              gCCTTTTTCATCAGGCTCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                          <  2:6613632/140‑1 (MQ=255)
              gCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                          <  1:10143890/140‑1 (MQ=255)
              gCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                          <  2:8275040/140‑1 (MQ=255)
              gCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                          <  1:2339053/140‑1 (MQ=255)
              gCCATTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                          <  1:10819873/140‑1 (MQ=255)
               ccTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATc                                                                                                                         <  2:695379/140‑1 (MQ=255)
               ccTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATc                                                                                                                         <  1:2568581/140‑1 (MQ=255)
                      catcaGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGAt                                                                                                                  <  1:10441952/140‑1 (MQ=255)
                      catcaGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGAt                                                                                                                  <  2:2432355/140‑1 (MQ=255)
                            gATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCtt                                                                                                            >  2:9691313/1‑140 (MQ=255)
                                gCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcag                                                                                                        <  1:1110321/140‑1 (MQ=255)
                                gCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcag                                                                                                        <  2:10147377/140‑1 (MQ=255)
                                 ccACACGGGTTTCCAGCTCGGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagc                                                                                                       <  2:2614151/140‑1 (MQ=255)
                                  cacaCGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagca                                                                                                      <  2:884413/140‑1 (MQ=255)
                                   acacGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGTTAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                     <  1:5828155/140‑1 (MQ=255)
                                   acacGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                     <  1:7788819/140‑1 (MQ=255)
                                   acacGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                     <  1:3916643/140‑1 (MQ=255)
                                   acacGGGTTTCCAGCTATGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCTGGATTTCAAACTATCCTAAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                     <  2:4855496/140‑1 (MQ=255)
                                         gTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGtt                                                                                               >  2:9409455/1‑140 (MQ=255)
                                          tttCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGACGCGGGATTTCAAACGATCCTCCACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGACTGAGGTGTTGATTACCTTCCAGCAGGGCGTTg                                                                                              >  2:7935307/1‑140 (MQ=255)
                                           ttCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGa                                                                                             <  1:1949635/140‑1 (MQ=255)
                                            tCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGCTCCTCAACGCCGTGGATCTCTTTCGGATAGCGACCCGAGGTGCGAATGATCTGGGGTTTACCTTCCAGCAGGGCGGTGaa                                                                                            >  1:2046724/1‑140 (MQ=255)
                                            tCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGaa                                                                                            >  1:5697428/1‑140 (MQ=255)
                                              cAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAgg                                                                                          <  2:4334362/140‑1 (MQ=255)
                                              cAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAgg                                                                                          <  2:4547707/140‑1 (MQ=255)
                                                gCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGgtg                                                                                        <  1:9385348/140‑1 (MQ=255)
                                                 ctctGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGgtgt                                                                                       >  1:5266988/1‑140 (MQ=255)
                                                    tGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGa                                                                                    <  1:4487422/140‑1 (MQ=255)
                                                         gTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAAc                                                                               <  1:9888734/140‑1 (MQ=255)
                                                          ttCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAAct                                                                              <  2:8654490/140‑1 (MQ=255)
                                                           tCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAActc                                                                             >  2:10853235/1‑140 (MQ=255)
                                                                  ccACAGTCAGTCCCCAACCGAAGAGGGATTTCAAAAGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTga                                                                      <  2:9771426/140‑1 (MQ=255)
                                                                      agtcagtcCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGTGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATc                                                                  >  1:8325923/1‑140 (MQ=255)
                                                                        tcagtcCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGt                                                                >  2:5856569/1‑140 (MQ=255)
                                                                          agtcCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagc                                                                                                       >  1:10023653/1‑99 (MQ=255)
                                                                          agtcCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagc                                                                                                       <  2:10023652/99‑1 (MQ=255)
                                                                           ttcCCCAACCGAAGAGTGATTTCAAACGATCCTCAACGCCGTTGATATCTTTCGGATAGCGACCCGAGGTGAGACTGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGTTGTGGAAAAACTATTCATGAGATCGTTCt                                                             >  2:1549080/2‑140 (MQ=255)
                                                                           gtcCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCt                                                             >  1:405468/1‑140 (MQ=255)
                                                                               ccAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTGGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTttat                                                         <  2:10474827/140‑1 (MQ=255)
                                                                                cAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTttatt                                                        >  1:4344824/1‑140 (MQ=255)
                                                                                 aaCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTttatta                                                       >  1:6239799/1‑140 (MQ=255)
                                                                                  aCCGAAGCGGGATTTCAATCGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAATACTCTTCCTGAGATCGTTCTTTATTAg                                                      >  1:27668/1‑140 (MQ=255)
                                                                                  aCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAg                                                      >  1:6742042/1‑140 (MQ=255)
                                                                                    cGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCa                                                    <  1:7919553/140‑1 (MQ=255)
                                                                                    cGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCa                                                    <  1:9691851/140‑1 (MQ=255)
                                                                                      aaGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaa                                                  <  2:5749799/140‑1 (MQ=255)
                                                                                      aaGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaa                                                  >  1:9175518/1‑140 (MQ=255)
                                                                                      aaGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaa                                                  >  1:240774/1‑140 (MQ=255)
                                                                                      aaGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaa                                                  >  2:10703827/1‑140 (MQ=255)
                                                                                      aaGCGGGATTTCAAACGATCCTAAACGCCGTTGATCTCTTTCGGATAGCGACCCGTGGTGAGTATGATATGTTGATTACCTTCGAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAgataa                                                  >  2:5209044/1‑136 (MQ=255)
                                                                                        gCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaaaa                                                >  2:10293769/1‑140 (MQ=255)
                                                                                           ggATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACt                                             <  1:503447/140‑1 (MQ=255)
                                                                                            gATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTg                                            >  1:7141900/1‑140 (MQ=255)
                                                                                             aTTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGa                                           <  1:9479504/140‑1 (MQ=255)
                                                                                                 cAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCATCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTATTTATTAGCAAAAAACTGAatat                                       <  2:6254889/140‑1 (MQ=255)
                                                                                                 cAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAatat                                       <  1:2929582/140‑1 (MQ=255)
                                                                                                 cAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAatat                                       <  1:362517/140‑1 (MQ=255)
                                                                                                 cAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAatat                                       <  1:8548579/140‑1 (MQ=255)
                                                                                                   aaCGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATAtcg                                     <  2:8544663/140‑1 (MQ=255)
                                                                                                   aaCGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATAtcg                                     <  1:3120774/140‑1 (MQ=255)
                                                                                                      gATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATAtcgtcg                                  >  1:8686572/1‑140 (MQ=255)
                                                                                                        tCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGAt                                <  1:6901784/140‑1 (MQ=255)
                                                                                                        tCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGAt                                <  1:8151629/140‑1 (MQ=255)
                                                                                                        tCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTGGACGGGGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATCTCGTCGAt                                <  2:3768733/140‑1 (MQ=255)
                                                                                                          cTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATca                              >  2:1953175/1‑140 (MQ=255)
                                                                                                           tCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATcag                             <  2:1234039/140‑1 (MQ=255)
                                                                                                            cAACGCCGTAGATCTCTTGCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATcagc                            <  1:7083154/140‑1 (MQ=255)
                                                                                                              aCGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATcagcag                          <  2:6803766/140‑1 (MQ=255)
                                                                                                              aCGCCGTTGATCTCTTACGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTACCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATcagcag                          <  2:7548226/140‑1 (MQ=255)
                                                                                                                 ccGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGc                       <  2:5064118/140‑1 (MQ=255)
                                                                                                                 ccGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGc                       <  2:3077860/140‑1 (MQ=255)
                                                                                                                  cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTCTTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCa                      >  2:6724516/1‑140 (MQ=255)
                                                                                                                  cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCa                      >  2:5362247/1‑140 (MQ=255)
                                                                                                                  cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCa                      >  2:6115240/1‑140 (MQ=255)
                                                                                                                  cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCa                      >  1:4884419/1‑140 (MQ=255)
                                                                                                                  cGTTGATCTCTTTCGGATAGCGAACCGAGGTGTGAATGATCTGTTGATTCAGTTCCAGCAGGGGGTGGAAGGTGTGGAAAATCTCTTCCTGAGATCGTTCTTTATTAGCAAAAATGTGAATATCGTCGATGAGCTGTGCa                      >  2:1806411/1‑140 (MQ=255)
                                                                                                                    ttGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGAt                                >  1:10610264/1‑128 (MQ=255)
                                                                                                                     tGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCt                   >  1:4131838/1‑140 (MQ=255)
                                                                                                                       aTCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTAc                 <  1:8935343/140‑1 (MQ=255)
                                                                                                                        tctcTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACgg               <  2:2273059/140‑1 (MQ=255)
                                                                                                                           ctTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa             <  1:3461863/140‑1 (MQ=255)
                                                                                                                           ctTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa             <  1:609506/140‑1 (MQ=255)
                                                                                                                           ctTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa             <  1:7354013/140‑1 (MQ=255)
                                                                                                                            tttCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAAc            >  2:3492802/1‑140 (MQ=255)
                                                                                                                            tttCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAACATCGTCGATCAGCAGTGCATCTACGGAAc            >  2:7356277/1‑140 (MQ=255)
                                                                                                                              tCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGTTCAGCAGTGAATCTACGGAACgg          >  2:594692/1‑140 (MQ=255)
                                                                                                                              tCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACgg          >  1:5442520/1‑140 (MQ=255)
                                                                                                                                  aTAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGgtagt      <  2:2016699/140‑1 (MQ=255)
                                                                                                                                  aTAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGgtagt      <  1:1670964/140‑1 (MQ=255)
                                                                                                                                    aGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGtagtag    <  1:1466271/140‑1 (MQ=255)
                                                                                                                                      cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATAtcg                                     >  2:2956297/1‑105 (MQ=255)
                                                                                                                                      cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGCg  >  2:3345549/1‑140 (MQ=255)
                                                                                                                                      cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGCg  >  1:3196699/1‑140 (MQ=255)
                                                                                                                                      cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGCg  >  2:3992807/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TGTCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGATCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGCG  >  NZ_CP025268/3884705‑3884978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: