Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,988,608 T→C *399* (TAA→TAG hemY ← protoheme IX biogenesis protein HemY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,988,6080TC99.6% 1954.7 / NA 561*399* (TAA→TAGhemYprotoheme IX biogenesis protein HemY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (401/158);  minor base A (2/0);  total (403/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 563 total aligned reads displayed.

ACCATGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACGTTGTTTCAGCG  >  NZ_CP025268/3988471‑3988744
                                                                                                                                         |                                                                                                                                        
acCATGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACCGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTa                                                                                                                                        >  2:7640812/1‑140 (MQ=255)
acCATGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGATCCGTAATAAGAGCAGGCGTTTTTTTATGTATCATGAAGGCCCCGGAGGTGCTTGCCTCCGTGTGAGAAGGAACTa                                                                                                                                        <  2:272603/140‑1 (MQ=255)
  cATGGACACAACGTTGAGTGACGAACACACTTGTTGTCATACAGACCTGTTTTAACGACTGCTCCGTAATAAGTGCAGGCGTATTTTAATGTATCAGGAAGGCCCAGGAGGTGCTTGACTCCGGGTTAGAAGGAactact                                                                                                                                      >  2:10828285/1‑140 (MQ=255)
   aTGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGTCCCCGGAGGTGATTGCCTCCGGGTGAGAAGGAACTACtg                                                                                                                                     >  2:7001400/1‑140 (MQ=255)
    tGGCCACCACGTTGAGTGAAGCACCCACGTGTTGTCATACAGACCTGTTTTAACGCCTGCGCCGTACTAAGAGCAGGCGTTGTTTTATGTATCAGGAAGGCCCCGGAGGTGCGTGCCTGCGGGGGCGCAGGAACGACtgt                                                                                                                                    >  2:1196068/1‑140 (MQ=255)
    tGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACtgt                                                                                                                                    >  1:5318203/1‑140 (MQ=255)
    tGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCGTGCCTCCGGGTGAGAAGGAACGACtgt                                                                                                                                    >  1:5687303/1‑140 (MQ=255)
     ggACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATTTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACtgtg                                                                                                                                   >  1:3061956/1‑140 (MQ=255)
     ggACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGGTTGCCTCCGGGGGAGAAGGAACTACtgtg                                                                                                                                   >  1:10264791/1‑140 (MQ=255)
     ggACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACtgtg                                                                                                                                   <  2:5225952/140‑1 (MQ=255)
     ggACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACtgtg                                                                                                                                   <  2:3236609/140‑1 (MQ=255)
          caACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCggg                                                                                                                              <  2:3604867/140‑1 (MQ=255)
          caACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCggg                                                                                                                              >  1:6084500/1‑140 (MQ=255)
            aCGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGtt                                                                                                                            >  2:10473903/1‑140 (MQ=255)
            aCGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGtt                                                                                                                            >  1:3237655/1‑140 (MQ=255)
            aCGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGtt                                                                                                                            >  1:9249966/1‑140 (MQ=255)
            aCGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAAAAAGAGCATGCGTTTTTTTATGTAAAAGGAAGGCCCCGGAGGTGCTTTCCAACGGGTGAGAAGGAACGTCTGTGGCCGGtt                                                                                                                            >  1:2943142/1‑140 (MQ=255)
                   gTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGTGAAGGAACTACTGTGGCGGGTTTTTCTGc                                                                                                                     >  1:3120727/1‑140 (MQ=255)
                       aGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACg                                                                                                                 >  1:725506/1‑140 (MQ=255)
                       aGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACg                                                                                                                 >  1:4602269/1‑140 (MQ=255)
                       aGCACCCACTTGTTGTCATACAGACATGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACg                                                                                                                 >  2:3827601/1‑140 (MQ=255)
                        gCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGt                                                                                                                <  2:9775707/140‑1 (MQ=255)
                         cACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGtt                                                                                                               <  1:3573148/140‑1 (MQ=255)
                         cACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGtt                                                                                                               >  1:7614651/1‑140 (MQ=255)
                         cACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACAGTGGCGGGTTATTAAGCAACGtt                                                                                                               >  1:5069813/1‑140 (MQ=255)
                            ccACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAAc                                                                                                            >  2:4412496/1‑140 (MQ=255)
                              aCTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACAt                                                                                                          <  1:6956073/140‑1 (MQ=255)
                                ttgttgTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCa                                                                                                        >  1:8282095/1‑140 (MQ=255)
                                 tgttgtCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCaa                                                                                                       >  1:4882315/1‑140 (MQ=255)
                                  gttgtCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCaaa                                                                                                      >  2:5766575/1‑140 (MQ=255)
                                     gtCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCg                                                                                                   >  2:7202641/1‑140 (MQ=255)
                                      tCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGACCTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGt                                                                                                  >  1:9726977/1‑140 (MQ=255)
                                      tCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGt                                                                                                  >  1:4213315/1‑140 (MQ=255)
                                        aTACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTcg                                                                                                <  2:8872595/140‑1 (MQ=255)
                                        aTACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTcg                                                                                                <  2:2772997/140‑1 (MQ=255)
                                        aTACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTATGGCGGGTTATTCTGCAACGTTAACATCAAACCGTcg                                                                                                >  2:900304/1‑140 (MQ=255)
                                             gACCTGTTTTAACGCCTGCTCCGTTATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCACCGGAGGTGCTTGCCTCCGGGTGAGAAGGAAATACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACcgtcgcgtcg                                                                                           >  2:4500730/1‑140 (MQ=255)
                                             gACCTGTTTTAACGCCTGCTCCGTCATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATGCTGCAACGTTAACATCAAACCGGCGCGACg                                                                                           >  2:1348814/1‑140 (MQ=255)
                                             gACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACg                                                                                           >  1:3357903/1‑140 (MQ=255)
                                             gACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACAGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACg                                                                                           >  1:2619133/1‑140 (MQ=255)
                                              aCCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCggg                                                                                                                              >  2:1688001/1‑104 (MQ=255)
                                              aCCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGc                                                                                          >  2:4863262/1‑140 (MQ=255)
                                              aCCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGc                                                                                          >  1:9015806/1‑140 (MQ=255)
                                              aCCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGc                                                                                          >  2:6186349/1‑140 (MQ=255)
                                                  gTTTTAACGCCTGCTCCGTCATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGCACTACTGTGGCGGGTTATTCTGCCACGTTAACATCAATCCGTCGCGACGCATAg                                                                                      >  2:7561524/1‑140 (MQ=255)
                                                  gTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAg                                                                                      >  1:8125216/1‑140 (MQ=255)
                                                  gTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAg                                                                                      >  1:3932320/1‑140 (MQ=255)
                                                   ttttAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGc                                                                                     >  2:5144547/1‑140 (MQ=255)
                                                       aaCGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCa                                                                                 <  2:2980458/140‑1 (MQ=255)
                                                         cGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGc                                                                               <  1:79808/140‑1 (MQ=255)
                                                             tGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGATAAGTAACTACTGTGGCGGGTTCTTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGcttct                                                                           >  2:7414510/1‑140 (MQ=255)
                                                             tGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGcttct                                                                           >  2:6457493/1‑140 (MQ=255)
                                                             tGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGcttct                                                                           >  2:914150/1‑140 (MQ=255)
                                                             tGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGcttct                                                                           >  2:4729745/1‑140 (MQ=255)
                                                               cTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCttcttc                                                                         >  1:8615663/1‑140 (MQ=255)
                                                                   gTAATAAGAGCAGGCGATTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGc                                                                     <  2:3076703/140‑1 (MQ=255)
                                                                       taaGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTtgt                                                                 <  2:6833110/140‑1 (MQ=255)
                                                                       taaGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTtgt                                                                 <  2:5526523/140‑1 (MQ=255)
                                                                       taaGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTtgt                                                                 <  1:9812570/140‑1 (MQ=255)
                                                                       taaGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAAACGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTtgt                                                                 >  1:1325231/1‑140 (MQ=255)
                                                                         agagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGc                                                               <  1:7363730/140‑1 (MQ=255)
                                                                         agagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGc                                                               <  2:8498935/140‑1 (MQ=255)
                                                                         agagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGc                                                               >  1:1750909/1‑140 (MQ=255)
                                                                          gagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGct                                                              >  2:2567597/1‑139 (MQ=255)
                                                                          gagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCa                                                              >  2:10707384/1‑140 (MQ=255)
                                                                          gagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCACCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCTTAGCTGCTGCTTCTTCCGGCTTGTGCa                                                              >  2:2704291/1‑140 (MQ=255)
                                                                            gCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGt                                                            <  1:150672/140‑1 (MQ=255)
                                                                            gCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGt                                                            >  2:7926963/1‑140 (MQ=255)
                                                                            gCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGt                                                            <  2:809687/140‑1 (MQ=255)
                                                                                gCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTATCATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGt                                                        >  2:4127964/1‑140 (MQ=255)
                                                                                   tttttttATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGTACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCa                                                     >  1:10848609/1‑140 (MQ=255)
                                                                                     tttttATGTATAAGGAATGCCCCGGAGGAGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGAGGGTTATTCTGCAACGTTAACATCAAACCGTCGGGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAgc                                                   >  2:8309946/1‑140 (MQ=255)
                                                                                          aTGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTc                                              >  1:7110867/1‑140 (MQ=255)
                                                                                            gtctCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCgg                                            <  2:6653154/137‑1 (MQ=255)
                                                                                              aTCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCa                                          >  1:1214368/1‑140 (MQ=255)
                                                                                                     aGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATg                                   >  2:1581388/1‑140 (MQ=255)
                                                                                                       gCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCggg                                                                                                                              >  1:2115237/1‑47 (MQ=255)
                                                                                                       gCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTCACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCg                                 >  1:9505056/1‑140 (MQ=255)
                                                                                                       gCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCg                                 <  2:9641254/140‑1 (MQ=255)
                                                                                                       gCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCg                                 >  1:6649402/1‑140 (MQ=255)
                                                                                                       gCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCg                                 >  1:7486824/1‑140 (MQ=255)
                                                                                                       gCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGGGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCTTAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCg                                 >  1:2823261/1‑140 (MQ=255)
                                                                                                        ccccGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGt                                >  1:6280553/1‑140 (MQ=255)
                                                                                                         cccGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTa                               >  2:7350452/1‑140 (MQ=255)
                                                                                                             gAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCg                           >  1:5166133/1‑140 (MQ=255)
                                                                                                             gAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTGAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCCAGCCATGCGTAATCg                           >  2:1928727/1‑140 (MQ=255)
                                                                                                                 tGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAgg                       >  1:7851762/1‑140 (MQ=255)
                                                                                                                 tGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAgg                       >  1:10669547/1‑140 (MQ=255)
                                                                                                                   cTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCg                     >  1:1893719/1‑140 (MQ=255)
                                                                                                                   cTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCg                     <  1:9183988/140‑1 (MQ=255)
                                                                                                                   cTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCg                     >  2:2183264/1‑140 (MQ=255)
                                                                                                                       ccTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCg                 >  1:1004269/1‑140 (MQ=255)
                                                                                                                         tCCGGGTGAGAAGGACCTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCTGCGCGTCGGCAAGCCATGCGTACTCGTAGGCGTCCGGa               >  2:3989857/1‑140 (MQ=255)
                                                                                                                           cGGGTGTGAAGGAACTACTGTGGCGGGTGATTCTGCAACGGTATCATCAAACCGTCGCGACGCATAGCTGCAGCGTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACg             >  2:10088750/1‑140 (MQ=255)
                                                                                                                           cGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACg             >  2:5959208/1‑140 (MQ=255)
                                                                                                                           cGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACg             >  1:2366788/1‑140 (MQ=255)
                                                                                                                            gggTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACgt            <  1:6453323/140‑1 (MQ=255)
                                                                                                                                  gaAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACGTTGTTTc      >  1:4391833/1‑140 (MQ=255)
                                                                                                                                   aaGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACGTTGTTTCa     >  2:9345139/1‑140 (MQ=255)
                                                                                                                                      gAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACGTTGTTTCAgcg  <  1:4160741/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ACCATGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACGTTGTTTCAGCG  >  NZ_CP025268/3988471‑3988744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: