Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,204,740 T→G V298G (GTA→GGA)  zraS → two‑component system sensor histidine kinase ZraS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,204,7400TG99.1% 1836.6 / ‑0.8 528V298G (GTA→GGA) zraStwo‑component system sensor histidine kinase ZraS
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (225/298);  total (225/303)
Fisher's exact test for biased strand distribution p-value = 7.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 528 total aligned reads displayed.

TGCCCACGAAATCCGTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGTAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCC  >  NZ_CP025268/4204603‑4204876
                                                                                                                                         |                                                                                                                                        
    cACGAAATCCGTAACCCACTTTCCTCGATTAAAGGTCTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGNATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGa                                                                                                                                    <  1:5804729/140‑1 (MQ=255)
    cACGAAATCCGTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTTCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGa                                                                                                                                    <  2:4462759/140‑1 (MQ=255)
             cGTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGa                                                                                                                           <  1:5572800/140‑1 (MQ=255)
              gTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGaa                                                                                                                          <  1:6078123/140‑1 (MQ=255)
              gTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGaa                                                                                                                          <  1:2989491/140‑1 (MQ=255)
              gTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGaa                                                                                                                          <  1:2870925/140‑1 (MQ=255)
              gTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGaa                                                                                                                          <  1:7861169/140‑1 (MQ=255)
              gTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGaa                                                                                                                          <  1:1146771/140‑1 (MQ=255)
                   ccACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGt                                                                                                                     <  2:10789768/140‑1 (MQ=255)
                    cACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGtt                                                                                                                    <  1:1287747/140‑1 (MQ=255)
                     aCTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTa                                                                                                                   >  1:479642/1‑140 (MQ=255)
                     aCTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTa                                                                                                                   >  1:7481437/1‑140 (MQ=255)
                      cTTTCCTCGATTAAAGGTCTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTaa                                                                                                                  <  1:4309985/140‑1 (MQ=255)
                      cTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTaa                                                                                                                  >  2:3094745/1‑140 (MQ=255)
                       tttCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCGACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAg                                                                                                                 >  1:8111473/1‑140 (MQ=255)
                       tttCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAg                                                                                                                 >  2:530171/1‑140 (MQ=255)
                             cGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGTAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACg                                                                                                           >  2:7356771/1‑140 (MQ=255)
                                 tAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATc                                                                                                       <  2:2193950/140‑1 (MQ=255)
                                   aaGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTg                                                                                                     >  1:705860/1‑140 (MQ=255)
                                   aaGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTg                                                                                                     >  2:3585525/1‑140 (MQ=255)
                                   aaGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTg                                                                                                     >  2:6805210/1‑140 (MQ=255)
                                         tGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTg                                                                                               >  2:5926761/1‑140 (MQ=255)
                                         tGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTg                                                                                               >  2:2575846/1‑140 (MQ=255)
                                          ggCGAAATTCTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGCTGGCGAAAGAGGCCGACCGTTTAAACCGCGGGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGc                                                                                              <  2:6160562/140‑1 (MQ=255)
                                          ggCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGc                                                                                              >  1:3680903/1‑140 (MQ=255)
                                          ggCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGc                                                                                              >  1:2054574/1‑140 (MQ=255)
                                          ggCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGc                                                                                              >  1:4677423/1‑140 (MQ=255)
                                          ggCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGc                                                                                              >  1:5674023/1‑140 (MQ=255)
                                          ggCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGc                                                                                              >  2:3828533/1‑140 (MQ=255)
                                          ggCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCAGGAACTGGTTAAGCCAACGCATCTGGCTTTGc                                                                                              <  2:10574728/140‑1 (MQ=255)
                                           gCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACTCATCTGGCTTTGCa                                                                                             >  2:8231715/1‑140 (MQ=255)
                                           gCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCa                                                                                             <  1:3956803/140‑1 (MQ=255)
                                             gAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAgg                                                                                           <  1:1595421/140‑1 (MQ=255)
                                               aaTACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCg                                                                                         <  2:6403337/140‑1 (MQ=255)
                                                aTACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCgg                                                                                        >  1:10081746/1‑140 (MQ=255)
                                                aTACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCgg                                                                                        <  1:5358141/140‑1 (MQ=255)
                                                aTACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCgg                                                                                        >  2:5460443/1‑140 (MQ=255)
                                                aTACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCgg                                                                                        <  2:1682165/140‑1 (MQ=255)
                                                  aCTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTGAAGCCAACGCATCTGGCTTTGCAGGCGGTg                                                                                      <  1:1926624/140‑1 (MQ=255)
                                                    tttGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGa                                                                                    <  2:6565922/140‑1 (MQ=255)
                                                     ttGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGACGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGAt                                                                                   >  2:2315757/1‑140 (MQ=255)
                                                     ttGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGAt                                                                                   >  1:8616058/1‑140 (MQ=255)
                                                           aGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGCACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAac                                                                             >  2:5771986/1‑140 (MQ=255)
                                                           aGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAac                                                                             >  2:4173574/1‑140 (MQ=255)
                                                           aGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAac                                                                             >  1:10256613/1‑140 (MQ=255)
                                                           aGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAac                                                                             >  1:10398929/1‑140 (MQ=255)
                                                           aGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAac                                                                             >  2:7866786/1‑140 (MQ=255)
                                                            gcgcgcgcCTGCAGGGGGAGAAGCGCATCATCTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAaca                                                                            <  2:9166883/140‑1 (MQ=255)
                                                            gcgcgcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGTGAAGCGAGTTGCTTGAACTGGTTAAGCCAACGCATATGGCTTTGCAGGCGGTGGATCTGaaaa                                                                            <  2:4730302/140‑3 (MQ=255)
                                                            gcgcgcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAaca                                                                            <  1:7265659/140‑1 (MQ=255)
                                                            gcgcgcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAaca                                                                            <  1:108592/140‑1 (MQ=255)
                                                            gcgcgcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAaca                                                                            <  1:5884347/140‑1 (MQ=255)
                                                            gcgcgcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAaca                                                                            <  1:997959/140‑1 (MQ=255)
                                                            gcgcgcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAaca                                                                            <  2:9300499/140‑1 (MQ=255)
                                                             cgcgcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTAAAACCGCGTGGGAAGCGAGGTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAacac                                                                           >  2:872187/1‑140 (MQ=255)
                                                              gcgcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACg                                                                          <  1:2488582/140‑1 (MQ=255)
                                                               ctcgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGc                                                                         >  2:5130486/3‑140 (MQ=255)
                                                                 cgcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTg                                                                       <  1:8704221/140‑1 (MQ=255)
                                                                  gcCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGAGAAAGAGGCCGACCGTTTAAACCGCGTGGGTAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGa                                                                      <  1:1398917/140‑1 (MQ=255)
                                                                      gCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTaa                                                                  <  1:1688799/140‑1 (MQ=255)
                                                                         gggggAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGTAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCa                                                               <  2:6098262/140‑1 (MQ=255)
                                                                         gggggAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCa                                                               <  1:5004147/140‑1 (MQ=255)
                                                                          ggggAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTACACCGCGTGGGAAGCGAGGTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCAc                                                              <  2:3455669/140‑1 (MQ=255)
                                                                            ggAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGa                                                                                    <  1:7662614/116‑1 (MQ=255)
                                                                              agaAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCAt                                                          <  1:6810525/140‑1 (MQ=255)
                                                                                aaGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTa                                                        <  1:4376037/140‑1 (MQ=255)
                                                                                aaGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTa                                                        <  2:10260927/140‑1 (MQ=255)
                                                                                  gcgcATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACa                                                      >  1:10537474/1‑140 (MQ=255)
                                                                                    gcATCATCTGGCGCAGGTGATGGCGAAAGTGGCCGACCGTTTAAACCGCGGGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGACTATCCACTCATTACAGc                                                    >  2:2150369/1‑140 (MQ=255)
                                                                                    gcATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGc                                                    >  1:4106595/1‑140 (MQ=255)
                                                                                     cATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGaa                                                                                                                          >  2:2941261/1‑69 (MQ=255)
                                                                                        cAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGt                                                >  2:3278377/1‑140 (MQ=255)
                                                                                             ggCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTc                                           >  2:7027661/1‑140 (MQ=255)
                                                                                             ggCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTc                                           <  1:9400456/140‑1 (MQ=255)
                                                                                             ggCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTc                                           >  1:2220141/1‑140 (MQ=255)
                                                                                                   ggggATGGCGAAAGAGGCCGACGGTGTAAACCGCGTGGGAAGCGCGTTGCTGGTCCTGGTGACGCCATCGCATGTGGCTGTGCAGGCGGTGGGTCTCTACACGCTGCGTTAGCACTCGTGACGGCTGGTCAGTCAGGGTg                                     >  2:8490971/4‑140 (MQ=255)
                                                                                                   ggTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATg                                     >  1:3854337/1‑140 (MQ=255)
                                                                                                    gTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAAcc                                                                <  2:5581515/112‑1 (MQ=255)
                                                                                                       aTGGCGCAAGAGGCCCACCGTTTAAACCGCGTGGGAAGCGAGTTGATGGAACGGGTAAAGCCAACGCATCTGGCTTTGCAGGCGGTGGCTCTCAAGACGCTGATGAACAACTCAGTCCTGCTGGTTATTCAGGATGCata                                 <  2:318230/140‑3 (MQ=255)
                                                                                                       aTGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCaaa                                 <  2:1476263/140‑1 (MQ=255)
                                                                                                         ggCGAAAGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACa                               >  1:6665419/1‑140 (MQ=255)
                                                                                                              aaGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCgg                          <  2:3960939/140‑1 (MQ=255)
                                                                                                              aaGAGGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCgg                          >  1:9474343/1‑140 (MQ=255)
                                                                                                               agagGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCggg                         <  2:5004086/140‑1 (MQ=255)
                                                                                                               agagGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCggg                         <  1:2658741/140‑1 (MQ=255)
                                                                                                               agagGCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGAAAGTCAGGATGCAAACAGCCggg                         <  1:4565372/140‑1 (MQ=255)
                                                                                                                  ggCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGgaga                      <  1:9130688/140‑1 (MQ=255)
                                                                                                                  ggCCGACCGTTTAAACCGCGTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGCAAGTCAGGACGCAAACAGCCGGgaga                      >  2:7212672/1‑140 (MQ=255)
                                                                                                                        ccGTTTAAACCGCGTGGGAAGCGAGTTGCTCGAACTGGTTAAGCTAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGt                <  2:9522043/140‑1 (MQ=255)
                                                                                                                             tacaCCGCGTGGGAAGCGAGTTGCTGGCACTGGTTAAGCCACCGCATCTGGCGTTGCAGGCGGGGGATCTCAACACGCTGATTAACCACTCATTACGGCTGGTAAGTCAGGATGCAAACAGCCGGGAGAGCCAGTTACGc           >  2:4827060/4‑140 (MQ=255)
                                                                                                                                  cgcgTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTAc      >  2:112148/1‑140 (MQ=255)
                                                                                                                                   gcgTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTAcc     >  1:5388286/1‑140 (MQ=255)
                                                                                                                                   gcgTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTAcc     >  2:7494715/1‑140 (MQ=255)
                                                                                                                                   gcgTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTAcc     >  1:3394325/1‑140 (MQ=255)
                                                                                                                                   gcgTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTAcc     >  1:1815183/1‑140 (MQ=255)
                                                                                                                                    cgTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCg    >  1:8902577/1‑140 (MQ=255)
                                                                                                                                     gTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGc   <  1:8287555/140‑1 (MQ=255)
                                                                                                                                     gTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGc   >  1:8798059/1‑140 (MQ=255)
                                                                                                                                     gTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGc   >  2:182846/1‑140 (MQ=255)
                                                                                                                                     gTGGGAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGc   >  2:9657874/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TGCCCACGAAATCCGTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGCGCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGTAAGCGAGTTGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCC  >  NZ_CP025268/4204603‑4204876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: