Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 374,358 T→C intergenic (+426/‑152) dmpG → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑(3‑hydroxy‑phenyl)propionate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268374,3580TC99.4% 1479.8 / ‑3.7 478intergenic (+426/‑152)dmpG/mhpT4‑hydroxy‑2‑oxovalerate aldolase/3‑(3‑hydroxy‑phenyl)propionate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (233/242);  minor base A (0/3);  total (233/245)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 486 total aligned reads displayed.

GGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCG  >  NZ_CP025268/374221‑374493
                                                                                                                                         |                                                                                                                                       
ggCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAgcgt                                                                                                                                       >  1:4183886/1‑140 (MQ=17)
 gcgcGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTc                                                                                                                                      >  2:2640224/1‑140 (MQ=17)
 gcgcGAGAATTTGTAGGCCAGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACCCCTAATCCGGCCTACGGAACGCGCGGGAATTAGTAGGCCTGATAAGACGCGTTAGCGTc                                                                                                                                      >  1:3001899/1‑140 (MQ=11)
  cgcgAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCg                                                                                                                                     <  1:3264169/140‑1 (MQ=17)
          tttGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGc                                                                                                                             <  1:922259/140‑1 (MQ=17)
            tGTAGGCCTGATCAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAt                                                                                                                           <  2:5892347/140‑1 (MQ=12)
            tGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAt                                                                                                                           <  2:8112183/140‑1 (MQ=12)
                 gtcTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGcg                                                                                                                      <  1:8422903/138‑2 (MQ=1)
                  ccTGATAAGACGCGTTAGCGTCGCTTCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGACTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGCATCTGcac                                                                                                                     >  2:5672672/1‑140 (MQ=11)
                  ccTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGcac                                                                                                                     >  1:25504/1‑140 (MQ=18)
                    tGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCCTCTGCacac                                                                                                                   >  2:9139910/1‑140 (MQ=255)
                      ctaccccgcgTTAGCGTCGCATCAGGCATCTGCGCACGACAGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCTGGAATTTGTAGGCCTGATAATACGCTTTAGCGTCGCATCAGGCATCTGCACACGa                                                                                                                 <  1:10244241/134‑1 (MQ=11)
                      aTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGa                                                                                                                 <  1:9630949/140‑1 (MQ=21)
                      aTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGa                                                                                                                 >  1:8755128/1‑140 (MQ=21)
                          gACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGc                                                                                                             <  1:3500005/140‑1 (MQ=21)
                            cgcgTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCg                                                                                                           >  2:7952197/1‑140 (MQ=21)
                             gcgTTCGCGTCGCAACCGGCATCAGCGCACGACTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCgg                                                                                                          <  2:3403346/140‑1 (MQ=11)
                               gTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGAt                                                                                                        <  2:974610/140‑1 (MQ=21)
                               gTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGAt                                                                                                        <  2:3154305/140‑1 (MQ=21)
                                ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATg                                                                                                       <  1:889696/140‑1 (MQ=21)
                                ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATg                                                                                                       >  2:8300229/1‑140 (MQ=21)
                                ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATg                                                                                                       >  2:825039/1‑140 (MQ=21)
                                ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATg                                                                                                       >  2:6372888/1‑140 (MQ=21)
                                    cGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACACCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGAt                                                                                                   >  1:3567833/1‑140 (MQ=255)
                                     gTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATa                                                                                                  <  2:1696522/140‑1 (MQ=37)
                                      tCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATaa                                                                                                 <  1:10005668/140‑1 (MQ=255)
                                       cGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACAAGACTGCCGGATGCGATaaa                                                                                                >  1:5984841/1‑140 (MQ=255)
                                           tCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTc                                                                                            <  1:52972/140‑1 (MQ=255)
                                               gCATCTGCGCACGACTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGt                                                                                        <  1:10706595/140‑1 (MQ=255)
                                                 aTCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTcc                                                                                      >  2:544587/1‑140 (MQ=255)
                                                  tCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCg                                                                                     >  1:7758610/1‑140 (MQ=255)
                                                    tGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGc                                                                                   >  2:3705293/1‑140 (MQ=255)
                                                    tGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGc                                                                                   >  2:8707476/1‑140 (MQ=255)
                                                    tGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGc                                                                                   >  1:2171504/1‑140 (MQ=255)
                                                    tGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGc                                                                                   >  1:7482811/1‑140 (MQ=255)
                                                    tGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGc                                                                                   >  1:9401111/1‑140 (MQ=255)
                                                      cgcACGACTGCCGGATGCGGCGTAATCGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATCAGACGCGTTAGCGTCGCTTCTGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCt                                                                                 >  2:4652841/1‑140 (MQ=17)
                                                      cgcACGACTGCCGGATGCGGAGTAAACGACTTATCCTGCATACGGATGGCGCGGGAATTTGTAGGCATGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCt                                                                                 >  2:4096924/1‑140 (MQ=17)
                                                            aCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCTCACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACAttt                                                                           <  2:4614118/140‑1 (MQ=14)
                                                             cTGCCGGATGCGGCGTAAACGGCTTATCCGGCCTACGGATGGCGCGGGACTTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTc                                                                          >  2:389880/1‑140 (MQ=255)
                                                              tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCg                                                                                                                                     <  2:7303115/80‑1 (MQ=21)
                                                              tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCg                                                                         >  2:2213194/1‑140 (MQ=255)
                                                                ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACAttt                                                                           >  2:8514656/1‑136 (MQ=255)
                                                                ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACAttt                                                                           <  1:8514657/136‑1 (MQ=255)
                                                                ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGcc                                                                       >  2:10671785/1‑140 (MQ=255)
                                                                ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGcc                                                                       >  1:1926287/1‑140 (MQ=255)
                                                                ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGcc                                                                       >  2:8978348/1‑140 (MQ=255)
                                                                 cGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGCTGGCGCGCGAATTTGTAGGACTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGccc                                                                      <  2:4517307/140‑1 (MQ=17)
                                                                  ggATGCGGCGTTAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCg                                                                     <  2:4984902/140‑1 (MQ=255)
                                                                     tGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAg                                                                  >  1:6395061/1‑140 (MQ=255)
                                                                       cggcgTCAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                                <  2:4256848/140‑1 (MQ=255)
                                                                       cggcgTAACCGCCTGATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                                <  1:159474/140‑1 (MQ=255)
                                                                       cggcgTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATNTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                                <  1:5807034/140‑1 (MQ=255)
                                                                         gcgTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAg                                                              >  2:6078138/1‑140 (MQ=255)
                                                                         gcgTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAg                                                              <  2:1530669/140‑1 (MQ=255)
                                                                         gcgTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAg                                                              <  1:4068127/140‑1 (MQ=255)
                                                                         gcgTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAg                                                              <  1:7877675/140‑1 (MQ=255)
                                                                          cgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGt                                                             >  1:8680766/1‑140 (MQ=255)
                                                                          cgTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGt                                                             >  2:6836837/1‑140 (MQ=255)
                                                                            ttaaCGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                           <  2:7106526/138‑1 (MQ=255)
                                                                            taaaCGCCTTATCCGGCCGACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                           <  1:9532683/138‑1 (MQ=255)
                                                                            gccactccTTCTCCGGCCTCCGGATGGCTCGAGACATTCCAGGGCTCAGAAGACGAGTTAGCGTCGCATCAGGCATCAGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                           <  2:7800024/134‑1 (MQ=18)
                                                                                 gCCTTCTCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                                                      <  1:1448802/140‑1 (MQ=255)
                                                                                 gCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                                                      <  1:10286151/140‑1 (MQ=255)
                                                                                 gCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                                                      <  1:5491656/140‑1 (MQ=255)
                                                                                 gCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                                                      <  1:6730630/140‑1 (MQ=255)
                                                                                 gCCTTATCCGGCCTACGGATGGCGCGGGAATTTGAAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAACTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                                                      <  2:2934451/140‑1 (MQ=255)
                                                                                      aTCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGtt                                                 >  1:1737156/1‑140 (MQ=255)
                                                                                        ccGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGGCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCt                                               <  1:7078052/140‑1 (MQ=255)
                                                                                               aCGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTAcg                                        <  1:9921788/140‑1 (MQ=255)
                                                                                                cGGATGGCGCGTGTATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAACAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTAcgc                                       <  1:1353617/140‑1 (MQ=255)
                                                                                                cGGATGGCGCGGGACTTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTAcgc                                       <  1:5140644/140‑1 (MQ=255)
                                                                                                cGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTAcgc                                       <  1:3377410/140‑1 (MQ=255)
                                                                                                cGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTAcgc                                       <  1:825039/140‑1 (MQ=255)
                                                                                                 ggATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTa                                                                                >  2:8211673/1‑98 (MQ=255)
                                                                                                  gATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCgg                                     <  1:5672907/140‑1 (MQ=255)
                                                                                                  gATGGCGAGTGTATGTGTCGGTCTGATTAGTCGCGTTAGCGTCGCATCTGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGCTTACGCgg                                     <  2:1849227/140‑1 (MQ=255)
                                                                                                   aTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGc                                    <  1:4452188/140‑1 (MQ=255)
                                                                                                    gggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCTCACGACTGCCGGATGCGATACACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCt                                   <  2:10783614/139‑1 (MQ=255)
                                                                                                     ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTg                                  >  1:4673503/1‑140 (MQ=255)
                                                                                                      gcgcGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTgg                                 <  1:482345/140‑1 (MQ=255)
                                                                                                      gcgcGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTgg                                 >  2:10504765/1‑140 (MQ=255)
                                                                                                      gcgcGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTgg                                 >  2:7563566/1‑140 (MQ=255)
                                                                                                      gcgcGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTgg                                 <  2:101292/140‑1 (MQ=255)
                                                                                                        gcgGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCa                               >  1:1093526/1‑140 (MQ=255)
                                                                                                        gcgGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCa                               >  1:3851368/1‑140 (MQ=255)
                                                                                                         cgGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCaa                              <  2:1361654/140‑1 (MQ=255)
                                                                                                                 tGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAg                      >  1:8134506/1‑140 (MQ=255)
                                                                                                                 tGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAg                      >  2:5199228/1‑140 (MQ=255)
                                                                                                                     ggCCTGCTCAGACGCGTTAGCGTCGCATCAGGCAACTGCACACGACGGCCGGATGCGCTAAACGTCTTGGCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTcgc                  <  2:7454861/140‑1 (MQ=38)
                                                                                                                      gCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTAcg                                        >  2:2404649/1‑117 (MQ=255)
                                                                                                                      gCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTAcg                                        <  1:2404649/117‑1 (MQ=255)
                                                                                                                       ccTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGa                <  1:7264396/140‑1 (MQ=255)
                                                                                                                           aTAAGACGCGTTAGCGTCGCATCAGGCCTCTGCACACGACTGCCGGATGCGCTAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCACTGTATCAGTCGCGATTCa            >  1:7806437/1‑140 (MQ=255)
                                                                                                                             aaGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCAcc          <  1:6589068/140‑1 (MQ=255)
                                                                                                                                   cgTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATGAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACt    <  1:7435764/140‑1 (MQ=255)
                                                                                                                                     ttAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCg  >  2:3652477/1‑140 (MQ=255)
                                                                                                                                     ttAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCg  >  2:6267350/1‑140 (MQ=255)
                                                                                                                                     ttAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCg  >  2:531992/1‑140 (MQ=255)
                                                                                                                                     ttAGCGTAGCATCAGGCCGCTGCACACGACTGCCGGATGCGATAAACGTGTTGTCCGGCCTCCATGTCGCCCGTAGGCGGTCATTCAGGAGGGCTGTTTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCg  >  2:9449565/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCG  >  NZ_CP025268/374221‑374493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: