BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,608,154	G→A	noncoding (48/87 nt)	CXP41_RS23870 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,608,154	0	G	A	98.0%	1054.1 / ‑6.5	313	noncoding (48/87 nt)	CXP41_RS23870	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (3/2); major base A (170/132); minor base T (6/0); total (179/134)
Fisher's exact test for biased strand distribution p-value = 3.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG  >  NZ_CP025268/4608020‑4608289

ccACGTCCGTAAGTACACTAACACCTGAAGCTTGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  2:1133613/140‑1 (MQ=255)

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     >  1:4940753/1‑140 (MQ=255)

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  2:1874304/140‑1 (MQ=255)

 cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                                                    >  1:6305666/1‑140 (MQ=255)

  aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                   <  2:2342154/140‑1 (MQ=255)

        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                                                                                             >  2:5580752/1‑140 (MQ=255)

        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                                                                                             >  1:3544653/1‑140 (MQ=255)

         tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCGCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                                                            >  1:6641947/1‑140 (MQ=255)

              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATTTCGCCTCGTTTGGT‑TCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                                                       <  1:8067918/140‑1 (MQ=255)

              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGTAAACTAACACCTGAAGCTa                                                                                                                       >  2:27492/1‑140 (MQ=255)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTATTTGAAATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTTAAACACCCCGTCCCTAAGAACACAAAAACCAGAAGCTAgc                                                                                                                     >  2:6879312/1‑140 (MQ=25)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACACACCATACTTTTATTGATATCCAAGCGATTGGT‑GCGAGGGGGGGGCGTTGAAGCCCAACGTCCGTAGGTACTCTAACACCTGTAGCTAga                                                                                                                     >  1:4521549/1‑139 (MQ=16)

                 cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                                                    <  1:1877398/140‑1 (MQ=255)

                 cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCGTACTTTTTTTGATATCGCCTCGGTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                                                    >  1:885385/1‑140 (MQ=255)

                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCAATCTTACTTTTTTTGATATCGCCGCGGTTGGT‑GCGAGGGGGGGGAATTTAACCACCACGTCCGTAAGAACACTAACACATTAAGCTacatc                                                                                                                   >  1:3317149/1‑136 (MQ=25)

                   aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                  >  1:5657587/1‑140 (MQ=255)

                    acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                                                                                                 <  2:3936687/140‑1 (MQ=255)

                      acCTTAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCATCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                                                               >  2:3150093/1‑140 (MQ=255)

                       ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                                                              >  1:4294819/1‑140 (MQ=255)

                        cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGGTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                                             >  2:1711026/1‑140 (MQ=255)

                         tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                            >  1:2161721/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  2:5665544/114‑1 (MQ=255)

                            aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACNTACACCTGACGCTAGCGCGTCTACCAAt                                                                                                         >  1:5770840/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GAGAGGGGGGGGGCTTGAACCCCCGCGTCCGTAAGTACACAAACCCCTGAAGCTAGCGCGGATACCAATTCCg                                                                                                     >  1:6971039/1‑140 (MQ=25)

                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    <  1:10003142/140‑1 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGACCACTAACACCTGTAGCTAGCGCGTCTACCAATTCCGcc                                                                                                   >  2:422008/1‑140 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                   >  2:8167017/1‑140 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                   >  2:75808/1‑140 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    <  2:2478345/139‑1 (MQ=255)

                                       cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                   >  2:8815858/1‑103 (MQ=255)

                                       cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                                                              >  2:621244/1‑140 (MQ=255)

                                          ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                                           <  2:10117576/140‑1 (MQ=255)

                                           cAATTCCGCGACCTTCGCACAGTCAACTTACTTTTTTTGATATCGACTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                                                          <  2:9479365/140‑2 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:2433105/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:8764909/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:863042/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:7747087/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:4749951/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTATATGATATCGACTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:6961086/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCAAATTCATCTTTATTTTTTTTTTATCGCCTCGTTTGGTGGGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                                           <  1:9562721/140‑1 (MQ=17)

                                                ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                     <  2:4838983/140‑1 (MQ=255)

                                                  gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                                   >  2:6590079/1‑140 (MQ=255)

                                                   ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                                                  >  1:10433408/1‑140 (MQ=255)

                                                    gaCCTTCGCACAGTCATCTTACTTTTTTTGAATTAGCCTCTTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 <  2:5770800/139‑1 (MQ=18)

                                                       cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa                                                                              >  2:7319717/1‑140 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTTAAGCTAGCGCGTCTTCCAATTCCGCCACCTTCGCCCAGTGCGAGCAAGATCAACggg                                                                    >  1:8300002/1‑138 (MQ=21)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACggg                                                                    >  2:1259432/1‑138 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTg                                                                    >  2:10280946/1‑140 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGAGAGAGCACTATCAACggg                                                                    >  2:7276680/1‑138 (MQ=18)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACATTCGCCAAGAGCGAGAACTATaaaagag                                                                    >  2:1552293/1‑136 (MQ=18)

                                                                    tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAAAGTGGtt                                                                 >  1:1235578/1‑140 (MQ=255)

                                                                         cTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTgg                                                            >  1:8898756/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                          >  1:3134784/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                          >  1:5960076/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGGTTGGTg                                                          >  1:4790445/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATACCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTg                                                          >  1:8454664/1‑140 (MQ=255)

                                                                             ttttGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                                        <  1:2621494/140‑1 (MQ=255)

                                                                                   tatCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTATCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                 <  2:10393943/140‑1 (MQ=255)

                                                                                   tatCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                 <  1:75808/140‑1 (MQ=255)

                                                                                   tatCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTGCGA‑ggggg                                                 <  2:1995238/140‑1 (MQ=255)

                                                                                   tatCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                 >  2:5175164/1‑140 (MQ=255)

                                                                                        ccTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACAGACATATCTATCAAAGTGGTTTTAGGTGCGA‑GGGGGGGGAc                                            >  1:2868854/1‑140 (MQ=14)

                                                                                          tCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACtt                                          <  1:7319718/140‑1 (MQ=255)

                                                                                            gTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTGGTGCGA‑gggggggggaatta                                        >  2:2235747/1‑134 (MQ=255)

                                                                                                  t‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAAccccc                                  <  2:8964962/140‑1 (MQ=255)

                                                                                                  t‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAAccccc                                  >  1:9897749/1‑140 (MQ=255)

                                                                                                   ggcgcGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCTGTTGGAGAATTATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAAccccc                                  <  1:5338771/139‑1 (MQ=18)

                                                                                                   gcgagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCAAGCGCGTCAACCAAATCCGCCACCATCGCCCAGTGCGAGAAATAAAAACGGTGTTTTAGGGGGGA‑GGGGGGGGACAGGAAccccc                                  >  2:6839217/6‑140 (MQ=14)

                                                                                                    gcgcGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGCCCAGTGCGAGCAATCTCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                 <  1:10101848/140‑1 (MQ=255)

                                                                                                    gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                 <  2:6305666/140‑1 (MQ=255)

                                                                                                     cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                     <  1:2393919/88‑1 (MQ=255)

                                                                                                      gAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACg                               <  1:1601874/140‑1 (MQ=255)

                                                                                                       aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGt                              >  1:5867753/1‑140 (MQ=255)

                                                                                                       aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGt                              >  2:9081150/1‑140 (MQ=255)

                                                                                                       aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGAGAGCAATATCAAAGTGGTTTTTGGTGCGA‑GGGTGGGTACATTAAACCACACGt                              >  2:4419239/1‑140 (MQ=18)

                                                                                                        ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTc                             >  1:5023960/1‑140 (MQ=255)

                                                                                                         gggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGt                                                                                      <  1:10179220/83‑1 (MQ=18)

                                                                                                           gggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGt                          <  1:1450340/140‑1 (MQ=255)

                                                                                                            ggggACTTGACCCCCCACTTCCGTAAGAACACTACCACCTGAAGCTAGCGCGTCTACCACGACCGCCACCTTCGCCCAGTGCTAGCAATCTCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTa                         <  1:6409736/140‑1 (MQ=25)

                                                                                                            ggggACATGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTa                         <  1:946478/140‑1 (MQ=255)

                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTaa                        >  2:4708376/1‑140 (MQ=255)

                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGGaa                        >  1:7478229/1‑140 (MQ=255)

                                                                                                              ggACTTGAACCCCCACGTCCGTCAGAACACTAACACCGGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                       <  1:7103242/140‑1 (MQ=255)

                                                                                                               gACTTGAACCCCCTCGTCCGTAAGAACAATAACACCTGACGCTAGCGCGTATACCTATTCCGCCACCTTCGCCCAGTGCGAGCAATATCATCGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAgg                      <  2:8473071/140‑1 (MQ=25)

                                                                                                               gACTTGAACCCCCACGTCCGTAATAACACTATAACATGATTCTAGCGAGTATACCCATACCTCCACATTCGCAAATTGCTAGCAATAACATAGAGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAgg                      <  2:8718447/140‑1 (MQ=16)

                                                                                                               gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAg                       <  1:9094570/140‑1 (MQ=255)

                                                                                                                  ttGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAAGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGaca                   >  1:5832832/1‑140 (MQ=255)

                                                                                                                  ttGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGaca                   <  1:3952348/140‑1 (MQ=255)

                                                                                                                    gcaCCCCCACGTCCGTCAGAACACTACCACCTGAAGCTATCGCGTCTACCATTTCCGCCACCTTCGCCCAGTGCGAGCAAGATCAAAGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 <  2:2715778/138‑1 (MQ=25)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTTGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 >  2:3583756/1‑140 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:9344471/68‑1 (MQ=14)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 <  1:3374542/140‑1 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 <  2:10063210/140‑1 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 >  2:2880099/1‑140 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCTCTATCAACGTGGTTGTTGGTGCGA‑GGGGGGGGACTGGACCCCCCACGTCCGGAAGGACACt                 >  2:10781471/1‑140 (MQ=21)

                                                                                                                      aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa               <  1:4708376/140‑1 (MQ=255)

                                                                                                                      aCCCCCACGTACGTAAGAACACTAACACCTGAAGCTAGAGCTTCTTCCAATTCCTCCACATTCGCCCAGTGCTAGCAATATCAAAGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa               <  2:7460095/140‑1 (MQ=25)

                                                                                                                          ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc           >  2:2608831/1‑140 (MQ=255)

                                                                                                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGaa                                       >  2:4170365/1‑110 (MQ=255)

                                                                                                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg         >  1:604962/1‑140 (MQ=255)

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG  >  NZ_CP025268/4608020‑4608289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`