Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	390,887	G→A	A356V (GCG→GTG)	CXP41_RS02035 ←	IS3‑like element IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	390,887	0	G	A	90.9%	11.2 / ‑5.6	11	A356V (GCG→GTG)	CXP41_RS02035	IS3‑like element IS3 family transposase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (9/1);  total (10/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACGCCGTTGCCCGCATTATTT  >  NZ_CP025268/390751‑390905                                                                                                                                         |                   ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGTTATAATTCAACACCg                 >  2:8399260/1‑140 (MQ=255) ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg                 >  1:5645636/1‑140 (MQ=17) ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg                 >  1:7997316/1‑140 (MQ=17) ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg                 >  1:8798899/1‑140 (MQ=17) ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg                 >  2:2021861/1‑140 (MQ=17) ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg                 >  2:5136377/1‑140 (MQ=17)   ggCTGATTGATCCTACCCACGTAATATGGACCCAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt               >  2:10714009/1‑140 (MQ=2)   ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt               <  1:2120971/140‑1 (MQ=11)   ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt               >  1:4489330/1‑140 (MQ=11)   ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt               >  1:633836/1‑140 (MQ=11)                tACCCACGTAATATGGACACCGGCCTAAGCGAGGTTCGTGGTTTCAAATTGTTCCGGACTGAGGCCGCCCCACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATCAGTACTCGCCGTTGCCCGCtttagtg  >  2:7832844/1‑137 (MQ=11)                                                                                                                                         |                   GGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACGCCGTTGCCCGCATTATTT  >  NZ_CP025268/390751‑390905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑