Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 514,858 T→C intergenic (‑35/‑111) qmcA ← / → fetA SPFH/Band 7/PHB domain protein/iron ABC transporter ATP‑binding protein FetA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268514,8580TC99.6% 2448.5 / NA 702intergenic (‑35/‑111)qmcA/fetASPFH/Band 7/PHB domain protein/iron ABC transporter ATP‑binding protein FetA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (424/274);  minor base A (3/0);  total (427/275)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 702 total aligned reads displayed.

CAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCTTC  >  NZ_CP025268/514721‑514993
                                                                                                                                         |                                                                                                                                       
 aaaGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCaaa                                                                                                                                      >  2:3700489/1‑140 (MQ=255)
   aGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAAt                                                                                                                                    <  1:7326534/140‑1 (MQ=255)
          tACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTgctgct                                                                                                                             <  1:4515025/140‑1 (MQ=255)
            cTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTg                                                                                                                           >  1:1986122/1‑140 (MQ=255)
                 cACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaa                                                                                                                      <  2:838748/140‑1 (MQ=255)
                  aCTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaaa                                                                                                                     >  2:794671/1‑140 (MQ=255)
                  aCTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaaa                                                                                                                     >  1:1823820/1‑140 (MQ=255)
                    tGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACa                                                                                                                   >  1:4124263/1‑140 (MQ=255)
                         gCCCTGCGGTACGNTTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATta                                                                                                              >  1:9324114/1‑140 (MQ=255)
                         gCCCTGCGGTACGATTTTGACACCCGCGCCGACACTGACCAGCGCGACAAAAATGAGAAGCGGGATCAAGAGAAGCATCGGAAAAACCTCCTGTTGTACCGTCCCTAATCCGCAAAATTGCTGCGTGATTACACAAAGta                                                                                                              <  2:622000/140‑1 (MQ=255)
                         gCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATta                                                                                                              >  2:4257158/1‑140 (MQ=255)
                         gCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATta                                                                                                              >  1:1181112/1‑140 (MQ=255)
                             tGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATtgctg                                                                                                                               <  1:4846715/119‑1 (MQ=255)
                              gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCt                                                                                                         >  1:4300544/1‑140 (MQ=255)
                                 gTACGATTTTGACACCCGCGCCGACATTGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAATCCTCCTGTTTTACCGTCAATAATAACGAAAATTGCTTCTTGATTAAACAAATTATACCTgtt                                                                                                      <  2:2610493/140‑3 (MQ=255)
                                 gTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                      <  1:2294003/140‑1 (MQ=255)
                                 gTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                      <  1:8839122/140‑1 (MQ=255)
                                   acctatttGATACACTCGCCGACATTGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTa                                                                                                    <  2:10630121/135‑1 (MQ=255)
                                    cGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTAc                                                                                                   >  1:9821615/1‑140 (MQ=255)
                                            acacCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAagag                                                                                           >  1:9078051/1‑140 (MQ=255)
                                              acCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAagagag                                                                                         <  1:543620/140‑1 (MQ=255)
                                                ccGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                                       <  2:2260922/140‑1 (MQ=255)
                                                 cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTc                                                                                      <  2:5686867/140‑1 (MQ=255)
                                                   cgcCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTccc                                                                                    >  2:5093330/1‑140 (MQ=255)
                                                    gcCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTcccc                                                                                   <  1:2882088/140‑1 (MQ=255)
                                                          aaTGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCCGCTTGATTAAACAAATTATACCTGATTACTg                                                                                                 <  1:6112868/120‑1 (MQ=255)
                                                                 aGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                      <  1:55181/140‑1 (MQ=255)
                                                                 aGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                      <  1:6473506/140‑1 (MQ=255)
                                                                  gcgcGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGaa                                                                     >  1:6653483/1‑140 (MQ=255)
                                                                   cgcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTAt                                                                              >  2:4877244/1‑130 (MQ=255)
                                                                   cgcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAg                                                                    >  1:8106015/1‑140 (MQ=255)
                                                                   cgcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAg                                                                    >  2:5042910/1‑140 (MQ=255)
                                                                   cgcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAg                                                                    >  2:8092719/1‑140 (MQ=255)
                                                                    gcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAgg                                                                   >  2:3084439/1‑140 (MQ=255)
                                                                      gACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGAt                                                                 <  2:8684121/140‑1 (MQ=255)
                                                                       aCAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                      >  1:2765009/1‑134 (MQ=255)
                                                                         aaaaaTGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATaaa                                                              <  2:4368970/140‑1 (MQ=255)
                                                                                agaATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGtttt                                                       >  2:9761562/1‑140 (MQ=255)
                                                                                  aaTCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTa                                                     >  2:4699545/1‑140 (MQ=255)
                                                                                  aaTCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTa                                                     >  1:3267438/1‑140 (MQ=255)
                                                                                     cGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTa                                                  >  1:8180221/1‑140 (MQ=255)
                                                                                                 aaGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAATATCAGaaaa                                      >  2:1648650/1‑140 (MQ=255)
                                                                                                 aaGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaa                                      <  1:5140554/140‑1 (MQ=255)
                                                                                                 aaGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaa                                      >  2:2125718/1‑140 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                      >  2:3575085/1‑75 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTc                                                                                      >  1:494132/1‑91 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                      >  1:7506395/1‑107 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                      <  2:7506394/107‑1 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGAt                                                                 >  2:10411802/1‑112 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaaa                                     >  1:7155660/1‑140 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaaa                                     >  1:1318707/1‑140 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaaa                                     >  1:10023819/1‑140 (MQ=255)
                                                                                                  aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTGTTAGTAAAAATCAGaaaat                                     >  1:8360737/1‑139 (MQ=255)
                                                                                                   gCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATtata                                                                                                            >  2:5816477/1‑68 (MQ=255)
                                                                                                     aTCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAggg                                  <  2:5371076/140‑1 (MQ=255)
                                                                                                     aTCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAggg                                  <  1:9989456/140‑1 (MQ=255)
                                                                                                      tCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGa                                 >  1:3823648/1‑140 (MQ=255)
                                                                                                      tCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCGGATTACTGAAAGAGAGt                                                                                        >  1:10103335/1‑85 (MQ=255)
                                                                                                       cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaaa                                     <  1:3618799/135‑1 (MQ=255)
                                                                                                       cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGaa                                >  2:3281801/1‑140 (MQ=255)
                                                                                                       cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGaa                                <  2:9310299/140‑1 (MQ=255)
                                                                                                       cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGaa                                >  2:6041652/1‑140 (MQ=255)
                                                                                                       cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGaa                                >  1:10410704/1‑140 (MQ=255)
                                                                                                        ggAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACa                                                                                                                   <  2:9858728/56‑1 (MQ=255)
                                                                                                        ggAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAAc                               >  1:10568087/1‑140 (MQ=255)
                                                                                                         gAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGACGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACa                              >  2:149862/1‑140 (MQ=255)
                                                                                                         gAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTCAAAATCAGAAAAAGGGAACa                              >  2:6608901/1‑140 (MQ=255)
                                                                                                         gAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACa                              >  1:3453506/1‑140 (MQ=255)
                                                                                                         gAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACa                              >  1:1622380/1‑140 (MQ=255)
                                                                                                         gAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACa                              >  1:1077039/1‑140 (MQ=255)
                                                                                                          aaaaaCCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAg                             <  2:9169352/140‑1 (MQ=255)
                                                                                                             aaCCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGa                          <  2:4807362/140‑1 (MQ=255)
                                                                                                             aaCCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGa                          <  1:8697179/140‑1 (MQ=255)
                                                                                                               cctcctGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGATTTCCCCCTTATTCCTGCGACGGATACACTGTTTTAAGTAAAAATCAGAAACAGGGAACAGCGagg                        >  2:2474371/1‑138 (MQ=255)
                                                                                                               cctcctGTTGTACCGTCCATAATCAGCAAAAGTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATg                        <  1:2115694/140‑1 (MQ=255)
                                                                                                                   ctGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                      <  2:6778165/58‑1 (MQ=255)
                                                                                                                      ttgtACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAg                     <  1:6871631/136‑1 (MQ=255)
                                                                                                                       ggtACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCAGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAACGGGAACAGCGATGCAGGaaaa                <  2:130047/139‑1 (MQ=255)
                                                                                                                         tACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCGCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAAtc              >  1:5957290/1‑139 (MQ=255)
                                                                                                                         tACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATa              <  2:3855057/140‑1 (MQ=255)
                                                                                                                         tACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATa              <  2:1948706/140‑1 (MQ=255)
                                                                                                                             gTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTg                                                                                                        >  1:2458924/1‑46 (MQ=255)
                                                                                                                             gTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTa                                                                               >  1:146445/1‑71 (MQ=255)
                                                                                                                             gTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTcc          >  2:1020087/1‑140 (MQ=255)
                                                                                                                             gTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTcc          >  2:7174091/1‑140 (MQ=255)
                                                                                                                              tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGc                                                                        <  1:7642930/77‑1 (MQ=255)
                                                                                                                              tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTa                                                  <  1:7000410/99‑1 (MQ=255)
                                                                                                                              tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAAt                                             <  2:2775172/104‑1 (MQ=255)
                                                                                                                              tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCt         >  2:7679453/1‑140 (MQ=255)
                                                                                                                              tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCt         >  1:3067488/1‑140 (MQ=255)
                                                                                                                              tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCt         >  2:6945925/1‑140 (MQ=255)
                                                                                                                              tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCt         >  1:7877731/1‑140 (MQ=255)
                                                                                                                              tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTGAGTAAAAATCAGAAAAAGGTCACAGCGATGCCGGaaaagcggcct         >  2:8812081/1‑133 (MQ=255)
                                                                                                                                cATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGNGAACAGCGATGCAGGAAAATAGTCCttt       >  1:5771650/1‑140 (MQ=255)
                                                                                                                                cATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCttt       >  1:4772132/1‑140 (MQ=255)
                                                                                                                                cATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCttt       >  1:9529993/1‑140 (MQ=255)
                                                                                                                                  tAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGc     <  2:1119235/140‑1 (MQ=255)
                                                                                                                                   aaTCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCt    >  2:7425497/1‑140 (MQ=255)
                                                                                                                                   aaTCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCt    >  1:3921821/1‑140 (MQ=255)
                                                                                                                                    aTCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCtt   <  2:9501239/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCTTC  >  NZ_CP025268/514721‑514993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: