BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,292,822	2 bp→GG	noncoding (36‑37/132 nt)	CXP41_RS06830 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,292,822	0	A	G	88.7%	698.7 / 105.0	412	noncoding (37/132 nt)	CXP41_RS06830	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (26/19); new base G (104/248); total (135/277)
Fisher's exact test for biased strand distribution p-value = 3.01e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
*	NZ_CP025268	1,292,823	0	A	G	99.3%	1046.0 / NA	412	noncoding (36/132 nt)	CXP41_RS06830	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (133/275); minor base T (1/2); total (135/277)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 412 total aligned reads displayed.

TAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCG  >  NZ_CP025268/1292688‑1292955

||

tAGCTCGGAGTCAGAGGTTTTGATTATTTAAGGAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGa                                                                                                                                  <  2:10316889/140‑1 (MQ=17)

tAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGa                                                                                                                                  <  1:7207025/140‑1 (MQ=255)

 gcggattcgcttgagagttcagggacttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                 <  2:10667938‑M1/104‑1 (MQ=255)

 aGCTCGGAGTACCAGGTTTTGATTATTAAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGaa                                                                                                                                 <  1:7613998/140‑1 (MQ=17)

  cggattcgcttgagagttcatggacttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTCGAAg                                                                                                                                <  2:4022030‑M1/105‑1 (MQ=255)

  cggattcgcttgagagttcagggacttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCACCCTTCGGGACGTTGCCTGAGGCAACGATCTCTCGCTTGCGCTCGAATCGAACCTTAGTCGAAg                                                                                                                                <  2:5565843‑M1/105‑1 (MQ=255)

  cggattcgcttgagagttcagggacttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                                <  2:5229024‑M1/105‑1 (MQ=255)

      ttcgcttgagagttcagggacttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                            >  2:3820995‑M1/32‑140 (MQ=255)

         gTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCa                                                                                                                         <  2:6246221/140‑1 (MQ=255)

         gTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCa                                                                                                                         <  2:3246606/140‑1 (MQ=255)

          tAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGAAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                        <  2:3137551/140‑1 (MQ=17)

           aaCAGGTTCTGATTCTCGAAGGAGGCGATGGTGGAGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                       <  1:9447039/140‑1 (MQ=14)

               gagttcagggacttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                 <  1:6379839‑M1/104‑1 (MQ=255)

                  tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGGCGAACCTTGGTCGAAGCTTCTCATCCGTccac                                                                                                                >  2:5949640/1‑138 (MQ=14)

                  tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGAATAATAAACTCATCGCGCTAAGCCCAACGGTACGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGAAGAACCTTTGTCGTAGCTTCTCATCCTTcccc                                                                                                                >  2:10828233/1‑140 (MQ=9)

                    tGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                              <  1:4717477/140‑1 (MQ=255)

                    tGATTAATTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                              <  2:709111/140‑1 (MQ=14)

                     gATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGct                                                                                                             <  1:3787095/140‑2 (MQ=255)

                     gATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGATCTCTCGCTTTCGCTCCATTCGAACCTTCGTCGAAGGTTCTCACCCTTCCCCgat                                                                                                             <  2:5137691/140‑3 (MQ=14)

                     agggacttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCATCACCGCa                                                                                                             <  2:4242275‑M1/124‑1 (MQ=255)

                       ggacttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATg                                                                                                           <  2:883642‑M1/126‑1 (MQ=255)

                         tattATGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         <  2:7736891/138‑1 (MQ=255)

                         attTAAGTAAGCGATGGTGGTGGGTGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         <  2:10006658/140‑1 (MQ=11)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         <  1:1806169/140‑1 (MQ=255)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                         <  1:10670843/140‑1 (MQ=14)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                         <  1:501746/140‑1 (MQ=14)

                         acttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         >  2:3371373‑M1/13‑140 (MQ=255)

                         acttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         >  1:121199‑M1/13‑140 (MQ=255)

                          tttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGc                                                                                                        >  2:2549043/1‑140 (MQ=11)

                           ttttgaaagtGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                       >  1:5656202‑M1/11‑140 (MQ=255)

                           ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                       <  1:1688878/140‑1 (MQ=11)

                           ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                       <  1:7917850/140‑1 (MQ=11)

                             aaGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGa                                                                                                     <  1:4934675/140‑1 (MQ=11)

                                gAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCGAAAGCAGGCGCTCAACTCGAACCTTGGTCGAAGCTTATCATCCTACCCCGCTAGGGCAGAata                                                                                                  >  1:10538726/1‑140 (MQ=11)

                                  aGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACATTGGTCGAATCATCTCATCCTTACCCGCTTGGGCAGAATAtt                                                                                                >  2:6502051/1‑140 (MQ=11)

                                    cGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                       >  2:2237798/1‑131 (MQ=11)

                                    cGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTTGTCGAAGCTTCTCATCCATACCCGCGAGGGCAGAATAATTg                                                                                              >  2:4853065/1‑140 (MQ=11)

                                        GGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTCCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTg                                                                                          >  1:6510621‑M1/1‑140 (MQ=255)

                                             TGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGGCGCTCAATTCGAACCTTTGTCGTACCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGAt                                                                                     <  2:1486457‑M1/140‑1 (MQ=255)

                                                   AAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGAttcgttt                                                                               >  2:3056397‑M1/1‑137 (MQ=255)

                                                     GGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCTTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGTGTCGAACCTTAGTCGAAGCTTCTCATCCTTCGCCGCATGGGCTGAATATTTGATTGCGGATTCGCTTga                                                                             >  2:5726038‑M1/1‑140 (MQ=255)

                                                     GGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                             >  1:5922286‑M1/1‑140 (MQ=255)

                                                     GGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                             >  1:5440218‑M1/1‑140 (MQ=255)

                                                      GATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGGGGATTCGCTTgag                                                                            <  1:2599241‑M1/140‑1 (MQ=255)

                                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCg                                                                     <  2:7355776/140‑1 (MQ=11)

                                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCg                                                                     <  1:51115/140‑1 (MQ=11)

                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCggg                                                                   <  2:668887/140‑1 (MQ=11)

                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCggg                                                                   <  2:8087567/140‑1 (MQ=11)

                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGACGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCggg                                                                   <  1:8111104/140‑1 (MQ=11)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAgggg                                                                  >  2:4129022/1‑140 (MQ=11)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCACAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGAGTCGTTTGAGAATTCCGGGGCtttt                                                             <  2:3776268/140‑1 (MQ=11)

                                                                      gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTg                                                            <  1:9002167/140‑1 (MQ=11)

                                                                      gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTg                                                            <  2:3654577/140‑1 (MQ=11)

                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGaaa                                                         <  2:163666/140‑1 (MQ=11)

                                                                          ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCGCTCGCGGGCGCTCAAGTCGAACCGTGGGCGAAGCTTCTAATCCGTCCCCGCGTGGGCCGAATAATTGATTGCGGATTCGTTTGAGAATTCCGGGGCGTTTGTAAg                                                        >  1:3664624/1‑140 (MQ=2)

                                                                            cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTg                                                      <  1:6948259/140‑1 (MQ=11)

                                                                            cGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTg                                                      <  2:8168067/140‑1 (MQ=11)

                                                                             gCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCTCGCGGGCGCTCAAGTCGAACCGTGGTCGAAGCGTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGGGTTTGTAAGTGa                                                     >  1:9579361/1‑140 (MQ=11)

                                                                              cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAATTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGGGAt                                                    <  2:4368915/140‑1 (MQ=11)

                                                                               ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtg                                                   <  1:7384689/140‑1 (MQ=11)

                                                                               ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCCGAATATTTGATTGCGGATTCGTTGGGGAGGTCCGGGGCTTTTGAAAGTGTtg                                                   >  2:7920776/1‑140 (MQ=11)

                                                                                 ggcGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggt                                                 <  2:6066602/138‑1 (MQ=11)

                                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                              <  1:4157721/140‑1 (MQ=11)

                                                                                    gggTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCAAGTCGAACCTTGGTCTAAGCTTCTCATCCTTCCCCGCTTGGGCAGATTATTTGATTGCGGATTCGTTTGAGAATTCCGTGGCTTTTGAAAGTGAtggtggt                                              <  1:7764897/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:1976975/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  2:943081/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:9110161/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAACTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:1130252/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCAAGTCGAAGCTTTGTCGAAGCTTCTCATCCTTAACCGCTTTGGCATAAAATTTGATTTCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgctgtgtc                                         >  2:5375032/1‑136 (MQ=11)

                                                                                              cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  2:1936653/140‑1 (MQ=11)

                                                                                              cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  2:1762845/140‑1 (MQ=11)

                                                                                              cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  1:5503370/140‑1 (MQ=11)

                                                                                              cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  1:2714395/140‑1 (MQ=11)

                                                                                                   gCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                               <  1:4828660/140‑1 (MQ=14)

                                                                                                   gCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                               <  1:10059896/140‑1 (MQ=14)

                                                                                                   gCAACGCCCTCTCGCTGGCGCTCCAGTCGAACCCTGGTCGAAGCTTCTCATCCGGCCCCGCTTGGCCAGAATATTTGATTCCGGATGCGTTTGAGAACTCCGGGGCTTTTGAAAGTGATGGTGCTGGGGGAAGGATTACt                               <  2:9185620/140‑1 (MQ=255)

                                                                                                     atcGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCGCTTGGGCAGAAGATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                             <  2:3558186/138‑1 (MQ=14)

                                                                                                     aaCGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                             <  2:4794185/140‑1 (MQ=14)

                                                                                                        gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGGTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcg                          >  1:286372/1‑140 (MQ=14)

                                                                                                            tctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCgggg                                                                  <  2:2838577/96‑1 (MQ=11)

                                                                                                            tctcGCTGGCGCTCAAGTCGAACCTGGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                      <  1:2187540/140‑1 (MQ=14)

                                                                                                               ctcTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATACTTACACGATTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCTGGGCTTTTGAAAGTTATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                   <  1:6795374/138‑1 (MQ=14)

                                                                                                               cGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                   <  1:4445894/140‑1 (MQ=14)

                                                                                                                 cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt                 >  2:1413950/1‑140 (MQ=14)

                                                                                                                 cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCt                 >  1:8502215/1‑140 (MQ=14)

                                                                                                                  cggCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt                <  1:5360609/139‑1 (MQ=14)

                                                                                                                   ggCGCTCAAGTCGAACCTTGGTCGAATCTTCTCATCCGTCCCCGCTTGGGCAGAATAGTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTATTCGTCGCTtcgctcctc               <  1:244432/140‑6 (MQ=14)

                                                                                                                   ggCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc               <  2:9702449/140‑1 (MQ=14)

                                                                                                                   gacgcACGAGCCGAACCTGAGCCGACGCTTCGACTCCTCCCCCGGATTGGCAGATGATTATATTGCGGATTCGCTTGAGCTTTACCTGACTTTAGTAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc               <  1:1379024/138‑1 (MQ=2)

                                                                                                                    gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg              <  1:9709496/140‑1 (MQ=14)

                                                                                                                    gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg              <  1:7692953/140‑1 (MQ=14)

                                                                                                                      gcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCAGCATGGGCAGAATCTTTGATTGCGGATTCGTTAGAGAATTCCGGGGCTGTGGAAAGTGATGGGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGcc            >  2:6962896/1‑140 (MQ=14)

                                                                                                                       cTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         >  1:4586702/1‑110 (MQ=14)

                                                                                                                        tcaagtcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt          <  1:3246395/134‑1 (MQ=14)

                                                                                                                          gAGCCGACCCGTCGTCGAAGCTTCGCATCCTTCCCCGCTTGGGCAGCATAGTTTATTGCGGAGGCGCTTGAGTGTTCAGGGCCTTTTGAAAGTGCTGGTGGTGGGGGAAGGATTACTCAGCGCTGGGCGCTTCGGCCTTc        <  2:8420697/140‑1 (MQ=2)

                                                                                                                          ccgCCGAACCCTAGACGAAGCTTCTCATCCTTCCCCGCAGGGGCAGACTAATTGATTGCGGAGTCGCTTGAGACTACAGCTACATTTGAAAGTGATGGTGGTGGGGGAAGTATTACTCAGCGCTGCGCGCTTCGCCCTTc        <  1:6050489/138‑1 (MQ=11)

                                                                                                                          aagtcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc        <  1:4499793/136‑1 (MQ=14)

                                                                                                                            gcataaCCTTAGTCGAAGCTTCTAATCCTTCACCGAATGGGCAGAATATTTTATTTCTGATTCGCTTGAGAGTTAAGGGACTTTTGTAAGTGATGGTTGTTGGGGAAGGATTACTCAGCGATGCGAGCTTCGCCCTTCgg      >  2:10506676/5‑140 (MQ=11)

                                                                                                                                aaCCTTTGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGAATTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCg  <  2:7488618/140‑1 (MQ=14)

                                                                                                                                aaCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCg  <  2:2032191/140‑1 (MQ=255)

||

TAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCG  >  NZ_CP025268/1292688‑1292955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`