Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,512,432 (A)9→8 pseudogene (599/907 nt) CXP41_RS18440 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,512,4240A.98.1% 1449.7 / 17.3 315pseudogene (607/907 nt)CXP41_RS18440hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (5/1);  new base . (179/128);  total (186/129)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 340 total aligned reads displayed.

CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGCGCG  >  NZ_CP025268/3512286‑3512560
                                                                                                                                          |                                                                                                                                        
  gcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATGACTACCGCCaaaa                                                                                                                                       >  2:207124/1‑140 (MQ=255)
   cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaa                                                                                                                                      >  2:4135458/1‑140 (MQ=255)
   cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGGGATAAGAGAGCTCGACTCGTTCCACAGCACTACCGCCaaaaa                                                                                                                                      >  2:693473/1‑140 (MQ=255)
   cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAAGGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaa                                                                                                                                      >  1:4151149/1‑140 (MQ=255)
    gTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaaa                                                                                                                                     >  2:2283141/1‑140 (MQ=255)
             aaTCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGt                                                                                                                           >  2:5041674/1‑140 (MQ=255)
              aTCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTc                                                                                                                          <  2:7932/140‑1 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  2:3591743/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  2:4107048/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  1:3859343/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  1:2154958/1‑140 (MQ=255)
                   aGCATGGTCACTTCGTTTTCCTCCACCACGGTCCAGATCACGGTATGGACATCGCCTTTAACAATGCGATGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCTCC‑AAGAAAAACATCGGTCATGAt                                                                                                                     >  2:110654/1‑140 (MQ=255)
                    gCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAtt                                                                                                                    >  1:4916829/1‑140 (MQ=255)
                     cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAttt                                                                                                                   >  1:209830/1‑140 (MQ=255)
                         gTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTg                                                                                                               <  1:1591999/140‑1 (MQ=255)
                          tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGGCATGATTTTCTGa                                                                                                              >  1:61105/1‑140 (MQ=255)
                             gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATcc                                                                                                           >  1:2548684/1‑140 (MQ=255)
                              ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCa                                                                                                                         >  1:177500/1‑125 (MQ=255)
                                 gTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                       <  2:54017/140‑1 (MQ=255)
                                   tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                                     >  2:128594/1‑140 (MQ=255)
                                    ttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAAc                                                                                                    <  2:3514341/140‑1 (MQ=255)
                                      ctgCCACCAGGTTCCAGATCACCGCATCGACATCGATTTGACCCATGCGTTGAATTCCCTCGTGACAAGAGGGATCGACTCTTTCCTCATCACTCCCGCC‑AAAAAAAACATCAGTCATGGTTTTCTGATCCGCCGAACgg                                                                                                  <  2:587540/138‑1 (MQ=255)
                                      ccGCCACCACGTTCCAGCTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGTTAAGAGAGATCGACCCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                                  <  2:177211/140‑1 (MQ=255)
                                      ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                                  <  2:3859343/140‑1 (MQ=255)
                                      ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                                  <  1:1449278/140‑1 (MQ=255)
                                      ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                                  <  1:1810978/140‑1 (MQ=255)
                                          caccacGTTCCAGATCCCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt                                                                                              <  2:4429931/140‑1 (MQ=255)
                                           accacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATc                                                                                             <  2:4009264/140‑1 (MQ=255)
                                            ccacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATcc                                                                                            >  1:4860556/1‑140 (MQ=255)
                                             cacGTTCAAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTAGTGATAAGAGAGATCGCCTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCa                                                                                           <  1:659084/140‑1 (MQ=255)
                                                 ttCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGccc                                                                                       >  1:1948270/1‑140 (MQ=255)
                                                   ccAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                     <  1:4076743/140‑1 (MQ=255)
                                                   ccAGATCACCGCATCGACATCGCCTTTAACAATGCGTGGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACAGCGGGCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                     >  2:2378602/1‑140 (MQ=255)
                                                     aGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                   <  2:1334010/140‑1 (MQ=255)
                                                          acggCATCGACATCGCCTTTAACAGTGCGTGGTAAACTGTCGTGATAAGAGAGCTCGCCTCGCTCCACATCACTACCGCCAAAAAAAAA‑ATCGGTCATGATTCTCTCATGCGCCGACCGCTCATCCACCCCCAcgcgact                                                                              >  2:3925142/4‑137 (MQ=255)
                                                          aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                              >  1:5083399/1‑140 (MQ=255)
                                                          aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                              <  1:2221405/140‑1 (MQ=255)
                                                          aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                              >  2:2639405/1‑140 (MQ=255)
                                                          aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACAGCGGTCAGGATTTTCTGATCCGCCGAACGGTGATCCAGCCCCACGCGCtt                                                                              >  2:1720380/1‑140 (MQ=255)
                                                            cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCTCCCAACGGTTATCCAGCCCCACGCGCTTcc                                                                            >  2:5061969/1‑139 (MQ=255)
                                                            cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                                            >  1:2534785/1‑140 (MQ=255)
                                                            cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                                            >  2:4845725/1‑140 (MQ=255)
                                                             gCATGGACGTCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGGCTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCAc                                                                           <  1:3604404/140‑1 (MQ=255)
                                                              cATCGACATCGGCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCTAGCCCCACGCGCTTCACg                                                                          >  2:3692177/1‑140 (MQ=255)
                                                              cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACg                                                                          >  2:1042061/1‑140 (MQ=255)
                                                                tCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGtt                                                                        >  1:973096/1‑140 (MQ=255)
                                                                      tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                                  <  1:2002303/140‑1 (MQ=255)
                                                                        gCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                                <  2:2196906/140‑1 (MQ=255)
                                                                             tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCt                                                           >  1:475975/1‑140 (MQ=255)
                                                                             tAACAAAGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTGCCACATCACTACCGCC‑AAAAAGAACATCGGACATGATTTTCTGATCCGCCGCACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCt                                                           >  1:878864/1‑140 (MQ=255)
                                                                              aaCAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCtt                                                          >  2:1439633/1‑140 (MQ=255)
                                                                                  aTGCGTTGTATACTCTCGTTATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTGTCCAGCCCCACGCGCTTCACGTTGGCGGACTGGCCTTTGCg                                                      >  2:1486241/1‑140 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACTGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                   >  1:3122838/1‑110 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AACAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  2:329839/1‑140 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  2:4608159/1‑140 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  2:3321447/1‑140 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGGTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  2:2946613/1‑140 (MQ=255)
                                                                                    gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTCTCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                    >  2:4240881/1‑140 (MQ=255)
                                                                                    gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                    >  2:4935462/1‑140 (MQ=255)
                                                                                    gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                    >  2:253204/1‑140 (MQ=255)
                                                                                    gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                    >  1:4289852/1‑140 (MQ=255)
                                                                                               tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTgg                                         >  2:2061611/1‑140 (MQ=255)
                                                                                               tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTgg                                         >  2:1846969/1‑140 (MQ=255)
                                                                                               tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTgg                                         >  1:501578/1‑140 (MQ=255)
                                                                                                 tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                       <  1:305075/140‑1 (MQ=255)
                                                                                                 tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTGACGGCAAATCAACTGGTg                                       <  2:4544902/140‑1 (MQ=255)
                                                                                                    tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCa                                                                                                                         <  2:5119627/55‑1 (MQ=255)
                                                                                                    tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                                    >  2:438523/1‑140 (MQ=255)
                                                                                                      atgagagagATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  1:4321707/137‑1 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                   >  1:562326/1‑91 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  1:3509320/140‑1 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  >  1:4498609/1‑140 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  >  1:530948/1‑140 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTCCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  1:2985107/140‑1 (MQ=255)
                                                                                                      aTAAGAGAGACCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  2:3807956/140‑1 (MQ=255)
                                                                                                      aTAAGACAGATCGCCTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  1:5194276/140‑1 (MQ=255)
                                                                                                        acgagagaTCGCCTCGTTCCACATCACTACCGCC‑AAACAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCa                                <  2:4716438/138‑1 (MQ=255)
                                                                                                        aagaaagaTCGACTCGTACCACCTCACTACCGCC‑AAACAAACCATCGGTCATGATTTTCTGACCCGCCGAACGGTTCTCCAGCCCCACGCGCTTCACCTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCa                                <  2:2819428/140‑1 (MQ=255)
                                                                                                          gagagaTCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAAc                              <  1:1268154/140‑1 (MQ=255)
                                                                                                           agagaTCGACTCGTTCCACATCACTACCGAC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg                             >  2:4766116/1‑140 (MQ=255)
                                                                                                              gaTCGACTCTTTCCACATCACTCCCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAg                          <  1:2399724/140‑1 (MQ=255)
                                                                                                              gaTCGACTCGTTCCACATAACTAACGCC‑AAAAAAAACATCGGTCATCATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCCCGTTTGCGGCCTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAg                          <  2:1390888/140‑1 (MQ=255)
                                                                                                               aTCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAgg                         >  1:4767224/1‑140 (MQ=255)
                                                                                                               aTCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAgg                         >  1:3318709/1‑140 (MQ=255)
                                                                                                               aTCGACTCGTTCCACAGCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGCTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGTTGCTCGCCCACGTAgg                         >  2:2776166/1‑140 (MQ=255)
                                                                                                                tCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgt                        >  2:3055259/1‑140 (MQ=255)
                                                                                                                   aCTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg                     >  2:319423/1‑140 (MQ=255)
                                                                                                                    cTCGTTCCACATCACTACCGCC‑‑AAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTACTCGCCAACGTAGGTGTGCg                   >  1:4726992/1‑140 (MQ=255)
                                                                                                                    cTCGTTCCACAGCACTACCGCC‑AAAAAAAACATCGGTCATAATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCCTCACGTTTGCGGACTCGCCTTTACGGCAAATCCACTGGTGCTCGCCAACGTAGGTGTGc                    <  2:340144/140‑1 (MQ=255)
                                                                                                                       gTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGc                 >  2:1578829/1‑140 (MQ=255)
                                                                                                                         tCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGccc               <  1:319423/140‑1 (MQ=255)
                                                                                                                         tCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACATTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGccc               >  1:2863424/1‑140 (MQ=255)
                                                                                                                                  cTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAg      >  1:2459214/1‑140 (MQ=255)
                                                                                                                                  cTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAg      >  1:3954348/1‑140 (MQ=255)
                                                                                                                                  cTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAg      >  1:1738717/1‑140 (MQ=255)
                                                                                                                                   tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGAGCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTCCGGCGAATCAACTGGTGCTCCCCAACGTAGGTGTGCGGCCCCAACTccgg      >  2:4491761/1‑138 (MQ=255)
                                                                                                                                   tACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc     >  1:1025525/1‑140 (MQ=255)
                                                                                                                                     ccGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgcgc   >  1:1539511/1‑140 (MQ=255)
                                                                                                                                     ccGCC‑AAAAAAAACATCGGTCACGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgcgc   <  2:2863674/140‑1 (MQ=255)
                                                                                                                                       gCCAAAAAAAAACATACGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGcgcg  <  1:4207014/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGCGCG  >  NZ_CP025268/3512286‑3512560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑