Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,982,878 (G)8→6 pseudogene (72‑73/1388 nt) yifK → amino acid permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,982,8720G.98.2% 1279.6 / 9.9 273pseudogene (66/1388 nt)yifKamino acid permease
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base . (170/98);  total (173/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NZ_CP0252683,982,8730G.98.1% 1246.2 / 15.9 273pseudogene (67/1388 nt)yifKamino acid permease
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base . (167/97);  total (173/100)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

CATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT  >  NZ_CP025268/3982733‑3983005
                                                                                                                                           ||                                                                                                                                    
cagttGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGCGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTg                                                                                                                                       <  2:1977296/137‑1 (MQ=255)
cATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTg                                                                                                                                       <  1:246644/140‑1 (MQ=255)
     gCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTgggggg                                                                                                                                  <  2:194411/140‑1 (MQ=255)
     gCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTgggggg                                                                                                                                  <  1:2741020/140‑1 (MQ=255)
       aaCACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggca                                                                                                                                <  2:2402601/140‑3 (MQ=255)
           cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCATACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggcaccat                                                                                                                            >  1:4432575/1‑134 (MQ=255)
           cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggcaccat                                                                                                                            >  2:2735219/1‑134 (MQ=255)
                   tcccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  2:5042844/2‑140 (MQ=255)
                   aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  1:3620963/1‑140 (MQ=255)
                   aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCAGCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  2:4444748/1‑140 (MQ=255)
                    cccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCgg                                                                                                                 >  2:581014/1‑140 (MQ=255)
                    cccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCgg                                                                                                                 >  2:2603466/1‑140 (MQ=255)
                     ccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGc                                                                                                                <  1:3510121/140‑1 (MQ=255)
                     ccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGc                                                                                                                <  2:3091615/140‑1 (MQ=255)
                     ccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGAAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGc                                                                                                                <  1:4272744/140‑1 (MQ=255)
                      cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GTGGGGCACCATTGGCGTCGGcc                                                                                                               >  1:2216165/1‑140 (MQ=255)
                      cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGcc                                                                                                               >  2:1035580/1‑140 (MQ=255)
                      cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGcc                                                                                                               >  2:3955156/1‑140 (MQ=255)
                       aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCt                                                                                                              <  1:3009309/140‑1 (MQ=255)
                        tcaCGATAACCGGTAACACCGGAAAGCCTGCAAACACAACACGAGGATCTATGGCAGACAACAAACCAGAGCCAACGCGTGGACTGGAAGCTCGACATATCGAGCAGATCGCCCT‑‑GGGAGGCACCAGTGGAGTCTGCCTg                                                                                                             >  2:843414/3‑140 (MQ=255)
                        tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATTGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCTACATATCGAACTCATCGCCC‑‑GGGGGGGAACCATTGGCGTCGGCCTg                                                                                                             >  2:1567841/1‑140 (MQ=255)
                        tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTg                                                                                                             <  2:186816/140‑1 (MQ=255)
                        tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTg                                                                                                             >  1:5157262/1‑140 (MQ=255)
                          aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGtt                                                                                                           >  2:391378/1‑140 (MQ=255)
                          aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGtt                                                                                                           >  1:943978/1‑140 (MQ=255)
                          aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGtt                                                                                                           >  2:807371/1‑140 (MQ=255)
                            gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                                         >  1:4908325/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                       >  1:120392/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGCCAGATAACAAACCAGAGCTACAGCGTGGGGTGGAAGCTCGACATATCGAACTCATCGCCC‑‑GGGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                       >  2:2883867/1‑140 (MQ=255)
                               aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg                                                                                                      >  1:432629/1‑140 (MQ=255)
                                 ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                    >  1:1895065/1‑140 (MQ=255)
                                 ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                    <  1:156775/140‑1 (MQ=255)
                                 ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                    <  1:2687270/140‑1 (MQ=255)
                                    gTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGACGCTCGACATATCGCACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgcc                                                                                                 >  2:1091474/1‑140 (MQ=255)
                                       acacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCTCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc                                                                                              >  2:1925503/1‑140 (MQ=255)
                                        cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                             <  1:4280377/140‑1 (MQ=255)
                                        cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                             <  2:1715126/140‑1 (MQ=255)
                                        cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                             <  1:2267925/140‑1 (MQ=255)
                                          ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt                                                                                           <  2:4185673/140‑1 (MQ=255)
                                           cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa                                                                                          >  1:844764/1‑140 (MQ=255)
                                            ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc                                                                                         >  1:3864730/1‑140 (MQ=255)
                                             gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        >  1:3306776/1‑140 (MQ=255)
                                             gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        <  1:391609/140‑1 (MQ=255)
                                             aaaaGCATGCAAACACAACACGAGTATTTATCGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACCCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        <  2:1280356/139‑1 (MQ=255)
                                                  cATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                                   <  2:47967/140‑1 (MQ=255)
                                                   aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTTGGCTGGAAGCTCGACGTATCGAACTTATCTCC‑‑GTGTGGGGCACGATTGGCTTGGGCCTGTTTATGGGGGCGGCCAGTACCCGGaaa                                                                                  >  2:1681973/1‑140 (MQ=255)
                                                    tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAAt                                                                                 >  2:4410324/1‑140 (MQ=255)
                                                     gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATg                                                                                >  2:4022277/1‑140 (MQ=255)
                                                     gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATg                                                                                >  2:354539/1‑140 (MQ=255)
                                                       aaaCACCACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggg                                                                              <  1:4789950/140‑1 (MQ=255)
                                                           acaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg                                                                          <  1:575988/140‑1 (MQ=255)
                                                             aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                        <  1:2210030/140‑1 (MQ=255)
                                                              acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                       <  2:3124086/140‑1 (MQ=255)
                                                              acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                       >  1:4085116/1‑140 (MQ=255)
                                                               cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCa                                                                      >  2:4743032/1‑140 (MQ=255)
                                                               cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCa                                                                      >  2:3454616/1‑140 (MQ=255)
                                                               cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTGATGGGGGCCGCCAGTACCCTGAAATGGGCCGGACCa                                                                      >  1:3972497/1‑140 (MQ=255)
                                                                acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt                                                                     <  2:4946013/140‑1 (MQ=255)
                                                                acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt                                                                     <  2:3307217/140‑1 (MQ=255)
                                                                   aGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                  <  2:4371367/140‑1 (MQ=255)
                                                                   aGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACAGATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                  <  1:1968569/140‑1 (MQ=255)
                                                                   aGGATTTATGGCAGATAACAAACCAGAGATAAAACGTCGGCTCGAAGCTCGACATATAGAACAAATCGCCCT‑‑GGGAGGCACCATTGGCGTAGGCCTGTTTATGGGGGCCGCCAGTACCCTGAACTGGGCCGGGCCATCCg                                                                  <  2:1349325/140‑1 (MQ=255)
                                                                     gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa                                                                >  2:52206/1‑140 (MQ=255)
                                                                       tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGCCATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAtt                                                              >  1:1688003/1‑140 (MQ=255)
                                                                       tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGGCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAtt                                                              >  2:2006630/1‑140 (MQ=255)
                                                                          aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgtt                                                           >  1:465460/1‑140 (MQ=255)
                                                                              cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc                                                       <  1:1376876/140‑1 (MQ=255)
                                                                                gATAACTAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAAGTCATCGCCC‑‑GGGGGGGCGCCATGGGCGGCGGCCGTTTGATGGGGGCGGCCAGTGCCGAGAAATGGGCGGGGCCATCCGTATGGTGGGCAta                                                     >  2:661096/1‑140 (MQ=255)
                                                                                gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCtg                                                     >  2:2445323/1‑139 (MQ=255)
                                                                                gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCta                                                     >  1:668186/1‑140 (MQ=255)
                                                                                gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCta                                                     >  2:3404073/1‑140 (MQ=255)
                                                                                gATAACAAACCAGAGCTACAGCGGGGGCTGGAAGCTCGACATATCGAACTCGTCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGACAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCta                                                     >  1:3109276/1‑140 (MQ=255)
                                                                                 aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCCTCCGTATTGTTGGCCtat                                                    >  1:1603555/1‑140 (MQ=255)
                                                                                     cAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatca                                                <  2:1163450/140‑1 (MQ=255)
                                                                                         ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGc                                            <  2:228322/140‑1 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        >  1:2822592/1‑92 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        <  2:2822577/92‑1 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                        >  2:2784068/1‑140 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGGTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGCACTGTTGGCCTATATCATCCCCggg                                        >  1:1021685/1‑140 (MQ=255)
                                                                                              gCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGc                                       <  2:147624/140‑1 (MQ=255)
                                                                                                    gCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCg                                 >  1:2904849/1‑140 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCTAACTCATCGCCCT‑‑TGGGTGCACCATTGGCGTCGGCCTGGTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCCTCCGTATTGTTGGCCTATATCATCCCCTGGCGGTTCGTCt                              >  2:2151740/1‑140 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                              <  1:4942579/140‑1 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCAGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCCGTTCGTCt                              >  2:4499728/1‑140 (MQ=255)
                                                                                                         ggCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTCTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCttt                            >  2:3582283/1‑140 (MQ=255)
                                                                                                            tGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGGCTTTTtc                         >  1:3250594/1‑140 (MQ=255)
                                                                                                              gAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcat                       >  1:5187227/1‑140 (MQ=255)
                                                                                                              gAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcat                       >  2:490688/1‑140 (MQ=255)
                                                                                                               aaGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc                      >  1:3199896/1‑140 (MQ=255)
                                                                                                               aaGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCGGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc                      >  1:455147/1‑140 (MQ=255)
                                                                                                                  cacGACATATCGAACTCACCGCCCT‑‑GGGGCGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGCACCCCGAAATGGGCCGGGCCATCCGTAGTGTTGGCCTATATCATCGCCGGGCTGTGCGTCTTTTGCATCATg                   <  2:3512253/138‑1 (MQ=255)
                                                                                                                    cGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg                 >  1:395249/1‑140 (MQ=255)
                                                                                                                               aCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      <  2:3840603/140‑1 (MQ=255)
                                                                                                                               aCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      >  2:3242269/1‑140 (MQ=255)
                                                                                                                               aCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      <  1:3404073/140‑1 (MQ=255)
                                                                                                                               aCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      <  1:3819334/140‑1 (MQ=255)
                                                                                                                               aCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATACGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      >  2:4583890/1‑140 (MQ=255)
                                                                                                                                   aTCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt  >  1:3591138/1‑140 (MQ=255)
                                                                                                                                   aTCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt  >  1:4518693/1‑140 (MQ=255)
                                                                                                                                   aTCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt  >  1:4127789/1‑140 (MQ=255)
                                                                                                                                           ||                                                                                                                                    
CATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT  >  NZ_CP025268/3982733‑3983005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑