BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,477,488	(G)_7→6	pseudogene (127/1816 nt)	CXP41_RS23185 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,477,482	0	G	.	99.5%	869.1 / ‑0.3	186	pseudogene (121/1816 nt)	CXP41_RS23185	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); major base . (68/115); minor base T (0/1); total (68/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

TTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATT  >  NZ_CP025268/4477343‑4477617

ttCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCg                                                                                                                                         <  2:676493/140‑1 (MQ=255)

    cTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGAGAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCggggg                                                                                                                                     >  1:4127437/1‑140 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  1:3231177/1‑140 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  1:3520455/1‑140 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  1:3571828/1‑140 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  2:1823325/1‑140 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  2:1531312/140‑1 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  2:74560/1‑140 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  1:2044734/140‑1 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  1:2601074/140‑1 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  2:676456/140‑1 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  1:3046483/140‑1 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  1:4201749/140‑1 (MQ=255)

     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  2:3499777/140‑1 (MQ=255)

          gAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGa                                                                                                                              <  1:2136364/140‑1 (MQ=255)

             ttCGTCATGAGCAAAATATCATATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATAAATGATTTTGCCCGTATTGCC‑AGGGGTCAGGATaa                                                                                                                           >  2:4037254/1‑140 (MQ=255)

             ttCGTCATGAGCAAAATAGCAGATTTAAATTATTCTCAACACATTGCATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATTAATGATTTTGCCCGTATTGCC‑TGGTGGCAGGATaa                                                                                                                           >  2:4038925/1‑140 (MQ=255)

               cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGACGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  1:2802411/1‑140 (MQ=255)

               cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  1:2941844/1‑140 (MQ=255)

                gTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAg                                                                                                                        <  2:4312390/140‑1 (MQ=255)

                gTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAg                                                                                                                        <  1:2468493/140‑1 (MQ=255)

                  cATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCTGACAATGTTAAACAAAAAAGTGAAGGTGTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGaa                                                                                                                      <  2:2111957/140‑1 (MQ=255)

                          aaaTATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATAtt                                                                                                              <  2:2619013/140‑1 (MQ=255)

                          aaaGATCAGATTTAAATTATTCCCGACACATTACCTTGGCCGACAATTAGAAACAAAAAAGTGGCGCTGTAAATACCTGGCGTGTCGGAACAAATGGTTTTGCCCGTATTGCC‑GGGGGGCAGCATAACAGAAGGAAtttt                                                                                                              <  2:2168397/140‑1 (MQ=255)

                           aaTATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATAttc                                                                                                             >  1:4516155/1‑140 (MQ=255)

                                cAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTTCC‑GGGGGGCAGGATAACAGAAGGAATAttctttct                                                                                                        >  2:2309260/1‑140 (MQ=255)

                                  gATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTcc                                                                                                      <  2:2783221/140‑1 (MQ=255)

                                       aaaTTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAg                                                                                                 <  2:4479148/140‑1 (MQ=255)

                                             ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTTTAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtttt                                                                                           >  1:1058956/1‑140 (MQ=255)

                                             ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCGCGTATTGCC‑GGGGGGCAGGATAACAGAAGGACTATTCTTTCTCCTGGAGCAtttt                                                                                           >  2:1882919/1‑140 (MQ=255)

                                             ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAGGGAATATTCTTTCTCCTGGAGCAtttt                                                                                           >  1:3348866/1‑140 (MQ=255)

                                             ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtttt                                                                                           >  2:1064075/1‑140 (MQ=255)

                                                tcAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTag                                                                                        <  1:1740177/140‑1 (MQ=255)

                                                tcAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTag                                                                                        <  1:3138009/140‑1 (MQ=255)

                                                  aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTagag                                                                                      >  2:2833605/1‑140 (MQ=255)

                                                       attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAgg                                                                                                                    <  2:3708579/105‑1 (MQ=255)

                                                       attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                                 >  2:1757858/1‑140 (MQ=255)

                                                         tacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTgg                                                                               >  1:5085129/1‑140 (MQ=255)

                                                           cattaGCCGATAATTTTAAACAAAAAAGTTAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                             <  2:2401018/140‑1 (MQ=255)

                                                           cattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                             <  1:1261642/140‑1 (MQ=255)

                                                           cattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                             <  1:3440995/140‑1 (MQ=255)

                                                           cattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                             <  2:1506625/140‑1 (MQ=255)

                                                           cattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTAGAGCATTTTTAGAGTTTTTGGCa                                                                             <  1:3114532/140‑1 (MQ=255)

                                                            attaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaa                                                                            >  1:1727080/1‑140 (MQ=255)

                                                             ttaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                           <  2:3901552/140‑1 (MQ=255)

                                                             ttaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                           <  1:1823325/140‑1 (MQ=255)

                                                              taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAg                                                                          >  1:354806/1‑140 (MQ=255)

                                                              taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAg                                                                          >  1:3864606/1‑140 (MQ=255)

                                                              taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGAGTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAg                                                                          >  2:3972915/1‑140 (MQ=255)

                                                                gCCGACAATTTTAAACAAAAAAGTGAAGTTTTAGATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATACTTAGAGTTTTTGGCAAAGat                                                                        >  2:102177/1‑140 (MQ=255)

                                                                gCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGat                                                                        >  2:3984627/1‑140 (MQ=255)

                                                                 ccGACATTTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGata                                                                       >  2:4852604/1‑140 (MQ=255)

                                                                 ccGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGata                                                                       <  2:4916824/140‑1 (MQ=255)

                                                                 ccGACAATTTTAAACAAAAAAGCGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑‑GGGGGCAGGATAACAGAATGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                      <  2:2876117/140‑1 (MQ=255)

                                                                  cGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCCTTTTTAGAGTTTTTGGCAAAGatat                                                                      <  2:89652/140‑1 (MQ=255)

                                                                  cGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                      <  2:519110/140‑1 (MQ=255)

                                                                  cGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                      <  1:3984627/140‑1 (MQ=255)

                                                                     cAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTa                                                                   >  2:4715041/1‑140 (MQ=255)

                                                                       agtgtAAACAAAAAAGTGAGGTTTTAGAGACCTGGCGTGTTGGAAGGAATGAGTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTGTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTAcc                                                                 <  2:5169519/136‑1 (MQ=255)

                                                                         tttAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCt                                                               <  2:4290066/140‑1 (MQ=255)

                                                                           tAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTgg                                                             >  1:3372649/1‑140 (MQ=255)

                                                                                            ttttAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGc                                            <  2:1523535/140‑1 (MQ=255)

                                                                                             tttACATACCTGGCGTGTTTGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGACTCTTCTTTCTCCTGGAGCATTTTTCGAGTTTTTCGCAAAGCTATTTACCCTGGGTTCTGTGGATTTCAGCa                                           <  2:628557/140‑1 (MQ=255)

                                                                                             tttAAAGACCTGGCGTGTTGGAATGAATGATTTTGCACGTATTG‑CTTGGTGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTACAGTTTTTGGCAAAAATATTTACCCTGGGTTATGTGGATTTTAGCa                                           <  2:3008969/140‑1 (MQ=255)

                                                                                               tAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                         <  1:2546774/140‑1 (MQ=255)

                                                                                               tAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                         <  1:3685099/140‑1 (MQ=255)

                                                                                                      cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCa                                  >  1:1140349/1‑140 (MQ=255)

                                                                                                      cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCa                                  >  1:2417167/1‑140 (MQ=255)

                                                                                                      cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCa                                  >  1:3009486/1‑140 (MQ=255)

                                                                                                        ggCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAAc                                >  1:1272706/1‑140 (MQ=255)

                                                                                                           gtgtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGaa                             <  1:287729/140‑1 (MQ=255)

                                                                                                           gtgtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGaa                             <  1:3189844/140‑1 (MQ=255)

                                                                                                            tgtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAg                            <  2:1443843/140‑1 (MQ=255)

                                                                                                            tgtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAg                            <  1:3292646/140‑1 (MQ=255)

                                                                                                              ttGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCg                          >  1:4955283/1‑140 (MQ=255)

                                                                                                              ttGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCg                          >  1:2848479/1‑140 (MQ=255)

                                                                                                               tGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCgg                         <  1:3093035/140‑1 (MQ=255)

                                                                                                                ggAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCggg                        <  1:1895938/140‑1 (MQ=255)

                                                                                                                ggAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCggg                        <  1:768914/140‑1 (MQ=255)

                                                                                                                 taatgaatgATTTTGCCCTTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGt                       <  2:1189035/139‑1 (MQ=255)

                                                                                                                 gaatgaatGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGATGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTATGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACCAAGCGGGt                       <  2:3110281/140‑1 (MQ=255)

                                                                                                                 gaatgaatGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGt                       <  2:3377933/140‑1 (MQ=255)

                                                                                                                 gaatgaatGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGt                       <  1:2596604/140‑1 (MQ=255)

                                                                                                                 gaatgaatGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGt                       <  2:1188917/140‑1 (MQ=255)

                                                                                                                      aatgaTTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGaaa                  >  1:832729/1‑140 (MQ=255)

                                                                                                                        tgaTTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAata                >  1:4756776/1‑140 (MQ=255)

                                                                                                                          aTTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatg              <  1:976001/140‑1 (MQ=255)

                                                                                                                            tttGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatgat            <  1:74560/140‑1 (MQ=255)

                                                                                                                            tttGCCCCTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTCGCAAACGCTCTAACGAAGCGGGTAGAAATatgat            <  2:2031747/140‑1 (MQ=255)

                                                                                                                              tGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg          <  1:4405034/140‑1 (MQ=255)

                                                                                                                               gCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGc         <  1:3577899/140‑1 (MQ=255)

                                                                                                                               gCCCGTATTGCC‑‑GGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCt        <  2:3831258/140‑1 (MQ=255)

                                                                                                                                cccGTATTTCCCGT‑GGGCAGGATAACAGAAGGAAGATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTGTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCt        <  2:4638295/140‑1 (MQ=255)

                                                                                                                                cccGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCt        <  1:1965862/140‑1 (MQ=255)

                                                                                                                                cccGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCGTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCt        <  1:2263858/140‑1 (MQ=255)

                                                                                                                                 ccGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTc       <  2:1971740/140‑1 (MQ=255)

                                                                                                                                 ccGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTc       <  2:5085129/140‑1 (MQ=255)

                                                                                                                                     aTTGCC‑GGAGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCatat   >  2:3674683/1‑140 (MQ=255)

                                                                                                                                      ttGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATAtt  <  1:4692487/140‑1 (MQ=255)

                                                                                                                                      ttGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATAtt  <  2:1934693/140‑1 (MQ=255)

TTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATT  >  NZ_CP025268/4477343‑4477617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`