BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,197	+G	intergenic (+50/+4)	CXP41_RS24355 → / ← CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,196	1	.	G	96.4%	1092.0 / 29.0	333	intergenic (+49/+5)	CXP41_RS24355/CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base . (5/7); new base G (145/176); total (150/183)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑T‑G‑ATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTC  >  NZ_CP025268/581057‑581330

 gCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑T‑g                                                                                                                                        >  1:604006/1‑140 (MQ=255)

                cGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGGAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTc                                                                                                                         >  1:211427/1‑140 (MQ=255)

                      ttGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAg                                                                                                                   <  2:1329878/140‑1 (MQ=255)

                        gCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCg                                                                                                                 <  2:2676416/140‑1 (MQ=255)

                        gCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCg                                                                                                                 <  1:2531338/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  2:2800004/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  1:1290821/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  1:4071320/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  1:3877634/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  1:3384198/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  2:491083/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTCCACCTGATATTGAGTTGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCCGCACGTCTTAAGCAAGCACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  2:378777/140‑1 (MQ=255)

                             aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                            <  2:580727/140‑1 (MQ=255)

                                gtgtTTATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  2:3544954/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  2:1637900/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  1:1829643/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  2:1373786/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  1:1435802/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  2:3255201/140‑1 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:3054110/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:2986885/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:1048548/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  2:1223948/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:2188016/1‑140 (MQ=255)

                                  gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                       <  2:2984098/140‑1 (MQ=255)

                                  gtTCATACATCAGCTGCACCTGATATTGAGTGGCCTATGAACCCTGTCATGGAGTAATCATTGGGATTATGCCGCAGCACGTCTAAAGCAAGAACATGATGC‑GGCT‑G‑G‑GTGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                       <  2:2888418/140‑1 (MQ=255)

                                   ttGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATtac                                                                                                      >  1:1338085/1‑140 (MQ=255)

                                   ttGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                       >  1:541032/1‑139 (MQ=255)

                                     gATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                    <  1:174234/140‑1 (MQ=255)

                                     gATACATCAACTACACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAACCATtacta                                                                                                    <  2:3672768/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  1:3552120/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  1:673227/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTCCCTGAAGCGGAAAACATTACTACa                                                                                                  <  2:4194392/140‑1 (MQ=255)

                                       caCATCAACTGCACTTGATATTGAGTCGCCTACGAACCCTGCCAGGGAGTAATCCTTGGCATTATGCCGCAGCACCTCTTAAGCAAGAACATGCTGC‑GGCT‑G‑G‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  1:1881318/139‑1 (MQ=255)

                                        aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                 >  2:3605332/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑TCG‑G‑ATGCTATTTTTTTTCTGAAGCGGAAAACATTACTACAGTAc                                                                                              >  2:4256064/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                              >  1:2263758/1‑140 (MQ=255)

                                               cTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGGCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTg                                                                                          >  1:2849258/1‑140 (MQ=255)

                                                  cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                       >  2:2343410/1‑140 (MQ=255)

                                                   aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                      <  1:3231569/140‑1 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGATAACATTACTACAGTACCTTGAACCt                                                                                     >  2:3441947/1‑140 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     >  1:4469374/1‑140 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAAACCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     <  2:805670/140‑1 (MQ=255)

                                                     cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCtt                                                                                    <  1:2813275/140‑1 (MQ=255)

                                                        atatTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGt                                                                                 >  1:4175608/1‑140 (MQ=255)

                                                            tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                             >  1:1134099/1‑140 (MQ=255)

                                                            tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                             >  2:1017310/1‑140 (MQ=255)

                                                            tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                             >  1:2752561/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                           >  1:4799576/1‑140 (MQ=255)

                                                               gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACa                                                                          >  2:2463179/1‑140 (MQ=255)

                                                                tGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAt                                                                         <  2:3054110/140‑1 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTTAAGCGGAAAACATTACTACAGTACCTTGAACCTTTGTTTTAACATtc                                                                       >  1:1174035/1‑140 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                       >  1:4874579/1‑140 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGCAATCATTGGGATTATGCCGCAGCCCGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                       >  2:5028037/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                      >  1:1475205/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                      >  1:1610238/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                      >  1:320056/1‑140 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                 <  2:5102101/140‑1 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATGTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                 <  2:1456536/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             <  1:3331018/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             <  2:2086316/140‑1 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACa                                                                                                          >  1:742684/1‑94 (MQ=255)

                                                                              tggcAGGGAGTAATCATTGGTATTATGCCGCAGCACGTCTTAAGCAATAACATGCTGC‑GGTT‑T‑G‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  2:3387824/137‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCCTTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTGTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATGCTCGAAATGctct                                                           <  2:2603330/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  2:884173/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  2:2238017/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  1:124857/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  1:873609/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  1:3656196/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  1:5118260/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  1:4617692/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  1:4267179/140‑1 (MQ=255)

                                                                               gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                          >  1:84716/1‑140 (MQ=255)

                                                                               gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTATTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                          >  2:4800164/1‑140 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTGAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                         <  2:687679/140‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                         <  1:3668112/140‑1 (MQ=255)

                                                                                 cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                        <  1:3591504/140‑1 (MQ=255)

                                                                                 cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                        <  2:3209182/140‑1 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                   <  1:1534369/140‑1 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                  <  1:343294/140‑1 (MQ=255)

                                                                                        tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                 >  1:2626912/1‑92 (MQ=255)

                                                                                        tAATCATTGGGATTATGCCGCAGCACGTCGTAAGCAAGAACATGCTGCGGG‑T‑G‑G‑AGGCTATTTTTTTACTGAAGCGGAAAGAATGGCTAGGGGACCTTGAAGCTTGGGTTGAAGATTCGCGAGGGGCTGTGAGAGaatgt                                                 >  2:1760764/1‑138 (MQ=255)

                                                                                          aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAAGATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGCAAACATTACTACAGTACCGTGAACCTGGGTTTTAACATTCTTGAAATGCTCTGAGAGGATAtgt                                               >  2:2729083/1‑140 (MQ=255)

                                                                                             aTTGGGATTATCCCGCAGCAATTCTTAAGCAAGCACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGCACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTCTATGTGtt                                            <  2:1526484/140‑1 (MQ=255)

                                                                                                  gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGcc                                       <  1:963914/140‑1 (MQ=255)

                                                                                                     tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGGGTTAAGCcttc                                    >  2:3947755/1‑140 (MQ=255)

                                                                                                      aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                   >  2:1980693/1‑140 (MQ=255)

                                                                                                       tGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttctt                                  >  2:1293804/1‑140 (MQ=255)

                                                                                                       tGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttctt                                  >  1:707029/1‑140 (MQ=255)

                                                                                                         ccGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCg                                >  1:94950/1‑140 (MQ=255)

                                                                                                           gcagcaCGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGa                              <  1:373065/140‑1 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                             >  2:446395/1‑140 (MQ=255)

                                                                                                                aCGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCtt                         <  1:4332871/140‑1 (MQ=255)

                                                                                                                 cGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                        >  1:4087373/1‑140 (MQ=255)

                                                                                                                 cGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTAGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGACTTCTTCGGAATCttt                        >  1:2865860/1‑140 (MQ=255)

                                                                                                                   tCTGAAGCATGAACAGCCTGC‑GG‑T‑T‑GTATGCTAGTTTTGTCCTGAAGGGGAAACCATTACTACAGTATCCTGAACCTTGGTTTTAATATTCTCGAACTGCCCGGAGAGTATATGTGTTAAGCCCTCTTCGTTATCTTTtg                      <  2:4353931/140‑1 (MQ=255)

                                                                                                                             gAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa            >  1:468326/1‑140 (MQ=255)

                                                                                                                             gAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa            >  1:3999821/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                   >  2:4065059/1‑100 (MQ=255)

                                                                                                                              aaCATGCTGC‑GG‑T‑TGG‑ATGATATTTTTTTCCTGAAGCGGAAAACATTACTTCAGTACCTTGAACATTGGTTTTAACATTCTCGAAATGCTCTGTGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg           <  2:2377824/140‑1 (MQ=255)

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑T‑G‑ATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTC  >  NZ_CP025268/581057‑581330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`