BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,146,507	+T	pseudogene (1028/1643 nt)	flgK →	flagellar hook‑associated protein FlgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,146,507	1	.	T	97.5%	1009.6 / 13.2	280	pseudogene (1028/1643 nt)	flgK	flagellar hook‑associated protein FlgK
Reads supporting (aligned to +/- strand): ref base . (3/4); new base T (135/138); total (138/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

ACGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAT  >  NZ_CP025268/1146368‑1146639

 cGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gt                                                                                                                                      >  1:1581304/1‑139 (MQ=255)

  gCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtg                                                                                                                                     <  1:1421132/140‑3 (MQ=255)

  gCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtg                                                                                                                                     <  1:1667461/140‑3 (MQ=255)

   cTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACACAGCCGGATTCGATGCTAACGGCGATGCCGGCGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAACAACAAA‑g‑gtga                                                                                                                                    >  2:1012090/1‑137 (MQ=255)

    ttGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgac                                                                                                                                   >  2:2288421/1‑136 (MQ=255)

    ttGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgac                                                                                                                                   >  2:3736885/1‑136 (MQ=255)

     tGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgacg                                                                                                                                  <  1:305796/140‑6 (MQ=255)

     tGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgacg                                                                                                                                  >  1:4064343/1‑135 (MQ=255)

      ggACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGt                                                                                                                                 >  2:722589/1‑140 (MQ=255)

          aaCTGGCGCTGGCAGTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCg                                                                                                                             <  2:5163823/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACCCCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  1:3860922/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACCCAAAGCCGGATTTGATGCTACCGGCGATGCCGGTGAAGATTTCTTTGCTATCGCTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:5049220/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  2:4065514/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  1:4645924/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  2:1674353/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  1:3662554/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  1:3633435/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  1:2138653/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCGTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  1:4309186/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCAGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTTCGAt                                                                                                                           <  2:4787398/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGACTTGATGGTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  1:3202012/140‑1 (MQ=255)

            cTGGCGCTGGCATGTGCCGAGGCTTTCAACACCCAACACCAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAATATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGGAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:4288979/140‑1 (MQ=255)

             tGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATc                                                                                                                          <  2:433180/140‑1 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGc                                                                                                                     <  2:3304025/140‑1 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGc                                                                                                                     >  1:3707834/1‑140 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGc                                                                                                                     <  1:3405729/140‑1 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGc                                                                                                                     <  2:2071598/140‑1 (MQ=255)

                      cATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACg                                                                                                                 <  1:2622352/140‑1 (MQ=255)

                       aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  2:262503/140‑1 (MQ=255)

                       aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  1:2751/140‑1 (MQ=255)

                       aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAAAACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  1:3326707/140‑1 (MQ=255)

                       aTTTGCCGAGGATTTCAACCCCCAACACAAAGCCGCATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  2:267231/140‑1 (MQ=255)

                       aTTTGACGAGGACTTAAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGACAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  2:3850069/140‑1 (MQ=255)

                        tttGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGt                                                                                                               <  1:4220321/140‑1 (MQ=255)

                         ttGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTa                                                                                                              <  1:1272553/140‑1 (MQ=255)

                               aGGCTTTCAACACCCAACACACAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        >  1:4617444/1‑140 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        >  2:1266434/1‑140 (MQ=255)

                                ggCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGC‑AAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                                      <  2:1461560/140‑1 (MQ=255)

                                    ttCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTc                                                                                                   <  1:1987658/140‑1 (MQ=255)

                                     tCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTcc                                                                                                  >  1:3392119/1‑140 (MQ=255)

                                      cAACACCCAACACACGGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTCTCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGACCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  2:1308841/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  2:4968791/140‑1 (MQ=255)

                                         cacCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCgg                                                                                              >  1:3161477/1‑140 (MQ=255)

                                           ccccACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTCCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            <  2:4467081/140‑1 (MQ=255)

                                           cccaacccaaAGCCGGATTTGATGCGAACGGCGATGGCGGTTAAGCTTGCTTTGCTATCGGTAAGCCCGCGGTTCTGCAACACACGAAACACAAA‑GAT‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            <  2:818627/140‑1 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGGAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCACGGTa                                                                                            >  2:4581608/1‑140 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            >  1:4731513/1‑140 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            >  2:1815714/1‑140 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            <  2:3649930/140‑1 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGGAACTGATGCCTCCGCGGTa                                                                                            >  1:53694/1‑140 (MQ=255)

                                            ccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTAc                                                                                           >  2:679892/1‑140 (MQ=255)

                                                    aaGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        >  1:2908980/1‑119 (MQ=255)

                                                    aaGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACa                                                                                   <  1:624232/140‑1 (MQ=255)

                                                    aaGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACa                                                                                   >  2:2735199/1‑140 (MQ=255)

                                                     aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAg                                                                                  >  1:1805197/1‑140 (MQ=255)

                                                     aGCCGGATTTGATGCTAACGGCGATGCCGGAGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAg                                                                                  >  2:4425894/1‑140 (MQ=255)

                                                      gCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGa                                                                                 >  2:2704863/1‑140 (MQ=255)

                                                           aTTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTGGCGAGCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACa                                                                            <  1:3956041/140‑1 (MQ=255)

                                                                   cTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCg                                                                    <  1:116978/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:3738194/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:3511001/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  2:482180/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  >  1:2063866/1‑140 (MQ=255)

                                                                            aTGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGAGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataat                                                           >  2:3197316/1‑140 (MQ=255)

                                                                             tGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataata                                                          <  1:788475/140‑1 (MQ=255)

                                                                                cGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATc                                                       >  1:471780/1‑140 (MQ=255)

                                                                                  gTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  1:4641153/1‑70 (MQ=255)

                                                                                  gTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAg                                                     >  2:1379983/1‑140 (MQ=255)

                                                                                      aGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGc                                                 <  2:1915036/140‑1 (MQ=255)

                                                                                           tCTTTGCTATCGGTAAGCCCGCGGTTCTGC‑AAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCa                                           <  2:773995/140‑1 (MQ=255)

                                                                                            cTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACg                                                                                                                 >  1:1640958/1‑70 (MQ=255)

                                                                                            cTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGGCa                                           >  2:1633000/1‑140 (MQ=255)

                                                                                                  tATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGcc                                     <  2:3426896/140‑1 (MQ=255)

                                                                                                  tATCGGTAAGCACGCGGTTCTGCAGAACACGAAAAACAAA‑G‑GTGACGTTGCGCTCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGAAGATCACCCgac                                     >  2:2576776/1‑138 (MQ=255)

                                                                                                          aGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGc                             >  2:2187842/1‑140 (MQ=255)

                                                                                                          aGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGc                             <  1:4412793/140‑1 (MQ=255)

                                                                                                           gCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCa                            >  2:3962486/1‑140 (MQ=255)

                                                                                                               gctgTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:3022005/137‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACACA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:2472656/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAC‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:288603/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:4219963/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:4858163/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:3789507/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:4964464/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:1047259/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:3542829/140‑1 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  1:1351892/1‑140 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  1:1491692/1‑140 (MQ=255)

                                                                                                                      tGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACtttt                 >  1:3935919/1‑140 (MQ=255)

                                                                                                                       gCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTa                <  2:939690/140‑1 (MQ=255)

                                                                                                                         aaaaCCCGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACg              <  2:85127/140‑1 (MQ=255)

                                                                                                                             cacGAAAAACAAAGG‑G‑GACGTTGGGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAAACAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:3116074/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGTGGTACGGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:3096908/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:4900257/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:322588/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:1858236/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:88415/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:3288956/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:4799736/1‑140 (MQ=255)

                                                                                                                                     aCAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAt  <  1:3330375/140‑1 (MQ=255)

ACGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAT  >  NZ_CP025268/1146368‑1146639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`