BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,224,095	+GA	coding (248/249 nt)	CXP41_RS06440 →	protein YmgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,224,094	1	.	A	99.0%	781.5 / ‑0.8	207	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (125/78); total (129/78)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*	NZ_CP025268	1,224,094	2	.	G	99.0%	687.5 / ‑0.9	207	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (127/78); total (129/78)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 220 total aligned reads displayed.

TCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑A‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGC  >  NZ_CP025268/1223955‑1224219

||

tCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCtt                                                                                                                                   >  2:4696239/1‑140 (MQ=255)

 cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGAATAAACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑a                                                                                                                                >  2:1845932/1‑140 (MQ=255)

  aGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑ag                                                                                                                               >  1:699745/1‑139 (MQ=255)

    gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaa                                                                                                                             >  2:4009671/1‑137 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTa                                                                                                                     <  2:1742398/140‑1 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTa                                                                                                                     <  1:4721552/140‑1 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAg                                                                                                                   >  1:412610/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAg                                                                                                                   >  2:4846589/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAg                                                                                                                   >  1:2244167/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAg                                                                                                                   >  1:3201095/1‑140 (MQ=255)

               gCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGGGTAAgg                                                                                                                  >  2:549539/1‑140 (MQ=255)

                  tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGttt                                                                                                               >  1:3859959/1‑140 (MQ=255)

                  tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGttt                                                                                                               >  2:2459768/1‑140 (MQ=255)

                  tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGttt                                                                                                               <  2:2599634/140‑1 (MQ=255)

                  tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGttt                                                                                                               >  1:2979047/1‑140 (MQ=255)

                       aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATCATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGaa                                                                                                          <  1:3121828/140‑1 (MQ=255)

                          aTGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATaa                                                                                                       >  1:869146/1‑140 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                  <  2:109955/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                  <  1:2127485/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                  <  2:485277/140‑1 (MQ=255)

                                    tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                             <  2:699745/140‑1 (MQ=255)

                                     gtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGt                                                                                            <  2:520568/140‑1 (MQ=255)

                                         ttctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCa                                                                                        <  2:2979047/140‑1 (MQ=255)

                                            tttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCa                                                                                     <  2:2951479/140‑1 (MQ=255)

                                              tctGATCTCTATCTGCACATATTGCAGTACATGGAAATGCATCAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                   >  1:4899162/1‑140 (MQ=255)

                                              tctGATCTCTATCTGCAACTATTGCAGGACATTGAACTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGAGCTCTCTTAGA‑‑ACTTGAGTAAGGCTTTAGAATAAACATCAACTGTATCACTAtt                                                                                   >  2:2269558/1‑139 (MQ=255)

                                              tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                   <  2:1186939/140‑1 (MQ=255)

                                              tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                   >  2:4868076/1‑140 (MQ=255)

                                                    ctctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATg                                                                             >  1:1710329/1‑140 (MQ=255)

                                                       tATCTGCAAATATTGCAGTACATTGAAATGAATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                          >  1:4915379/1‑140 (MQ=255)

                                                         tCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAg                                                                        >  1:4052391/1‑140 (MQ=255)

                                                             cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                    <  1:3393616/140‑1 (MQ=255)

                                                             cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                    <  1:765589/140‑1 (MQ=255)

                                                             cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGAAAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                    <  1:2849402/140‑1 (MQ=255)

                                                              aaaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGc                                                                   >  1:1891227/1‑140 (MQ=255)

                                                                 tatTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCAtt                                                                >  1:3275443/1‑140 (MQ=255)

                                                                 tatTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCAtt                                                                >  1:131540/1‑140 (MQ=255)

                                                                   ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                              <  2:2223105/140‑1 (MQ=255)

                                                                   ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                              <  2:3512062/140‑1 (MQ=255)

                                                                      cAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAgg                                                           >  2:50861/1‑140 (MQ=255)

                                                                           cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                      >  1:1809942/1‑140 (MQ=255)

                                                                           cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                      >  1:2350196/1‑140 (MQ=255)

                                                                           cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                      >  2:2177320/1‑140 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                    <  2:3047621/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                    <  1:2177358/140‑1 (MQ=255)

                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                  <  1:4084259/140‑1 (MQ=255)

                                                                                aaaTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCtt                                                 >  1:3122157/1‑140 (MQ=255)

                                                                                 aaTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCttt                                                <  2:229692/140‑1 (MQ=255)

                                                                                   tGCATAAGACTACACTAACGGATATCATTATTAGTGACAGGGAATCAGGGCTTTCTTAGA‑‑ACTTGAGTAACGTTATAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGGGAGCAATTCAGGCGTTATGCTTTCt                                              >  2:85674/1‑140 (MQ=255)

                                                                                   tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                              >  2:2462165/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                            >  2:303217/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                            >  2:4415285/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                            >  1:2658651/1‑140 (MQ=255)

                                                                                          gACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                              <  1:2252832/117‑1 (MQ=255)

                                                                                          gACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTCAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATGTCAGGCGTTATGCTTTCTTatgatg                                       >  2:4999221/1‑140 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                    <  2:571358/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                    <  2:345269/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                    <  1:1984236/140‑1 (MQ=255)

                                                                                              acacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                   <  2:869146/140‑1 (MQ=255)

                                                                                              acacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                   <  1:4009671/140‑1 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                  >  2:1933505/1‑140 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                  >  1:1026096/1‑140 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                  >  2:1605247/1‑140 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                  >  1:4134110/1‑140 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                  >  2:242071/1‑140 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                  >  1:4476463/1‑140 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCGTTCTTATTATGTCCGc                                  >  1:4290129/1‑140 (MQ=255)

                                                                                                  tAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAat                               <  1:2571491/140‑1 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATTCTTTCTTATTATGTCCGCAatat                             >  2:2819217/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                             <  1:2793046/140‑1 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                             >  2:4940296/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                             >  1:4540895/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                             <  1:1431825/140‑1 (MQ=255)

                                                                                                     cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                            >  2:2874115/1‑140 (MQ=255)

                                                                                                     cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                            <  1:4868076/140‑1 (MQ=255)

                                                                                                       gATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAg                          >  1:3651100/1‑140 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                    <  1:633906/96‑1 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                        >  1:2460642/1‑140 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                        >  2:3673935/1‑140 (MQ=255)

                                                                                                          atCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtg                       >  2:2119296/1‑140 (MQ=255)

                                                                                                          atCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGCATCACCATGCTGATGCAAAGTGAGCATTTGAGACGGTATGATTTCTTATTATGTCCGCAATATCAGatg                       >  2:180728/1‑140 (MQ=255)

                                                                                                           tCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtgt                      <  2:4039527/140‑1 (MQ=255)

                                                                                                              ttattaATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                   >  2:4549806/1‑140 (MQ=255)

                                                                                                              ttattaATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                   >  1:1190910/1‑140 (MQ=255)

                                                                                                              ttattaATGACAGGGAATCCGTGCTCTCTT‑‑AGCACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                   >  2:1309451/1‑140 (MQ=255)

                                                                                                                  taATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                  >  2:4357204/1‑105 (MQ=255)

                                                                                                                  taATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                  <  1:4357204/105‑1 (MQ=255)

                                                                                                                  taATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAAt               >  1:2558909/1‑140 (MQ=255)

                                                                                                                      gACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag           >  2:1384580/1‑140 (MQ=255)

                                                                                                                       aCAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACGTCAGCTGTATCACCATGCTGATGCAAATTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgaga          >  2:3944424/1‑140 (MQ=255)

                                                                                                                         aGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGt        >  2:1250347/1‑140 (MQ=255)

                                                                                                                           ggAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc      >  1:4787261/1‑140 (MQ=255)

                                                                                                                             aaTCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTACAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtctc    <  2:3361117/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg   <  1:5213040/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg   <  1:4413266/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg   <  1:1082218/140‑1 (MQ=255)

                                                                                                                               tCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGc  >  2:5044873/1‑140 (MQ=255)

                                                                                                                               tCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGc  >  1:3318699/1‑140 (MQ=255)

                                                                                                                                   tGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGAt                                                                              <  1:3837837/60‑1 (MQ=255)

                                                                                                                                   tGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGAt                                                                              >  2:3837837/1‑60 (MQ=255)

||

TCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑A‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGC  >  NZ_CP025268/1223955‑1224219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`