BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,374,862	(T)_8→9	intergenic (+40/‑172)	pspE → / → ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,374,854	1	.	T	94.3%	1075.7 / 46.3	302	intergenic (+32/‑180)	pspE/ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand): ref base . (8/9); new base T (139/144); total (147/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 317 total aligned reads displayed.

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGC  >  NZ_CP025268/1374716‑1374988

gACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCACTTATGCGCCG‑‑‑t                                                                                                                                          >  2:223394/1‑140 (MQ=255)

  cGCCAGTCAGGGCAAGCAAAAGAGATCCTTATCGAGGTGGAAGATACACACGTTGAGAACGCCGGTGGCCTGAAGGAAATGGTAATGCCGAAGGTGAACGGTTAAATGAATTCAACATGAGAGGAGCTTAGGCGCCG‑‑‑t‑t‑t                                                                                                                                      >  2:1232943/1‑140 (MQ=255)

  cGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑‑t‑t‑t                                                                                                                                      >  2:1699164/1‑140 (MQ=255)

    ccAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑‑t‑t‑ttt                                                                                                                                    <  2:540909/140‑1 (MQ=255)

             gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTc                                                                                                                           >  1:732513/1‑140 (MQ=255)

             gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTc                                                                                                                           <  1:2493240/140‑1 (MQ=255)

              caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTca                                                                                                                          <  2:602490/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTcac                                                                                                                         <  1:4801318/140‑1 (MQ=255)

                agcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTcaca                                                                                                                        <  2:4616898/140‑1 (MQ=255)

                  caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACAcc                                                                                                                      <  1:4630160/140‑1 (MQ=255)

                      agagATCCTTAGCGAGATGGGATATACGCACGTTGAGAACGCCGGTGGCCTGAAGTAGATCGCAATGCCGAAGGTGAAAGGGTAAAAGAAGTGACCATGAGCGGCGCTTATGCGGCGT‑‑T‑T‑TTTTT‑TCTGTCCCAGCTTAt                                                                                                                  >  2:182359/1‑140 (MQ=255)

                         gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGGCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTAtttg                                                                                                               <  2:420419/140‑2 (MQ=255)

                          ccccctAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTCGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCCCCACGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTAtttcc                                                                                                              <  2:159082/135‑3 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCACTGCCGAAGGTCAAAGGTTAAACGAATTCCCCCTGAGCGGCGCTTCTGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACa                                                                                                             <  2:1656935/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACa                                                                                                             <  1:4131501/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACa                                                                                                             <  1:3643326/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACa                                                                                                             <  2:4864827/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGGTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTAcc                                                                                                             <  1:1274923/140‑2 (MQ=255)

                           tCCTTAGCGAGATGGGACATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGCGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACa                                                                                                             <  2:321883/140‑1 (MQ=255)

                            ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACAt                                                                                                            >  2:4633425/1‑140 (MQ=255)

                            ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACAt                                                                                                            <  1:2052185/140‑1 (MQ=255)

                               tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATccc                                                                                                         >  1:470170/1‑140 (MQ=255)

                               tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑‑t‑t‑ttttt‑ttctgt                                                                                                                           <  2:4414605/121‑6 (MQ=255)

                               tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑‑t‑t‑ttttt‑ttctgt                                                                                                                           >  1:4414605/1‑116 (MQ=255)

                                aGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGATCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATccac                                                                                                        <  2:1783112/140‑3 (MQ=255)

                                 gCGAGATGGGATCTACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCa                                                                                                       <  2:3661488/140‑1 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGTACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCTCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCa                                                                                                       <  2:691247/140‑1 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCa                                                                                                       <  2:4286311/140‑1 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCa                                                                                                       >  2:5118406/1‑140 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCa                                                                                                       >  2:4926554/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAACAGAATTCACCATGAGCGGCGCTTGTGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTGATTTACATCCCCATAGat                                                                                                  >  2:4431502/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGat                                                                                                  >  2:238463/1‑140 (MQ=255)

                                       tGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGatt                                                                                                 >  1:699423/1‑140 (MQ=255)

                                       tGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGatt                                                                                                 >  1:362736/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTT‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCg                                                                                          >  2:1488059/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                         >  1:1497006/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                         >  2:2476493/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGACGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                         <  2:4547024/140‑1 (MQ=255)

                                                   aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                     >  2:3923435/1‑140 (MQ=255)

                                                   aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                     >  1:2417638/1‑140 (MQ=255)

                                                   aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                     >  1:1002959/1‑140 (MQ=255)

                                                   aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                     >  1:3718421/1‑140 (MQ=255)

                                                    cGTTGAGAACTCCGGTGGCCCGAAAGACATCGCAAGGCCGACGGTCAAAGGTTAAAAGAAGTTACCATTAGCCTCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATGTACATCACAAGAGATTCGTTGGGTCAGcc                                                                                    >  2:3302725/1‑139 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                    >  1:1861297/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                    >  1:1927670/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                    >  1:5181220/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                    >  1:5026928/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                    >  1:3210147/1‑140 (MQ=255)

                                                     gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACa                                                                                                             >  1:1419126/1‑114 (MQ=255)

                                                     gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACa                                                                                                             <  2:1418747/114‑1 (MQ=255)

                                                         agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaa                                                                               >  2:2161626/1‑140 (MQ=255)

                                                         agaACGCCGGTGCCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaa                                                                               >  2:5151336/1‑140 (MQ=255)

                                                          gaACGCCGGTGGCCTGAAAGACATCGCGATGCCGAAGGTCAAAGGTTAGAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑‑T‑TACTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTTAACTCACaaa                                                                              >  2:3803259/1‑140 (MQ=255)

                                                           aaCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAt                                                                             >  1:390259/1‑140 (MQ=255)

                                                           aaCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAt                                                                             >  2:4812034/1‑140 (MQ=255)

                                                             cGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGTTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATAc                                                                           >  2:2746272/1‑140 (MQ=255)

                                                               ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGc                                                                         >  1:3946993/1‑140 (MQ=255)

                                                                        tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                    >  2:1459574/1‑120 (MQ=255)

                                                                              aCATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaa                                                          <  2:1060024/140‑1 (MQ=255)

                                                                                  cgaaaTGCTGAAGGTAAAAGGTTAACAGAATCCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                      <  2:3341884/137‑1 (MQ=255)

                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                      <  1:1356070/140‑1 (MQ=255)

                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                      >  1:3466016/1‑140 (MQ=255)

                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                      <  2:3831855/140‑1 (MQ=255)

                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                      >  1:2974639/1‑140 (MQ=255)

                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc                                            >  2:2884495/1‑140 (MQ=255)

                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc                                            >  1:2468501/1‑140 (MQ=255)

                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                           <  2:362690/140‑1 (MQ=255)

                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                           <  1:2408799/140‑1 (MQ=255)

                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCGGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                     >  1:1764443/1‑140 (MQ=255)

                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                     >  2:203530/1‑140 (MQ=255)

                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                     >  1:4013164/1‑140 (MQ=255)

                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                     >  1:3266221/1‑140 (MQ=255)

                                                                                                    aGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaa                                    <  1:2723349/140‑1 (MQ=255)

                                                                                                    aGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaa                                    <  1:4368999/140‑1 (MQ=255)

                                                                                                      gTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa                                  <  2:4333096/140‑1 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACAcc                                                                                                                      >  2:2596956/1‑53 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACAcc                                                                                                                      <  1:2596956/53‑1 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                               >  2:1373945/1‑140 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                               >  1:2850082/1‑140 (MQ=255)

                                                                                                           aaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt                             <  1:275476/140‑1 (MQ=255)

                                                                                                             gAATTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                           <  1:4875084/140‑1 (MQ=255)

                                                                                                             gAATTCACCATGAGCGGCGCTTATGCGACGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                           <  1:2815285/140‑1 (MQ=255)

                                                                                                               aTTCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGc                         <  2:2722316/140‑1 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGTTTT‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgtg                          <  2:4962987/140‑1 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACAcc                                                                                                                      >  1:3424419/1‑46 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                        >  1:216725/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                        >  2:1879634/1‑140 (MQ=255)

                                                                                                                    ccATGAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt                    <  1:1646348/140‑1 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACGAATACGCTTTTTCCCTGGTAAAAAAGGATTTCCTGCGTGACTAAAACCCGTGTGCTCAATTGAc                <  2:3718964/140‑1 (MQ=255)

                                                                                                                         aGCGGCGCTTATGCGCCGTT‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                <  1:3592023/140‑1 (MQ=255)

                                                                                                                             gcgcTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGttt           >  1:4469093/1‑140 (MQ=255)

                                                                                                                               gcTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt         >  1:3537066/1‑140 (MQ=255)

                                                                                                                               gcTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTAGTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt         >  2:2278094/1‑140 (MQ=255)

                                                                                                                                cTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAACCCCTTGTGCTCAATTGACAGTTTAtt        <  1:857368/140‑1 (MQ=255)

                                                                                                                                cTTATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtt        <  2:3438065/140‑1 (MQ=255)

                                                                                                                                  tATGCGCCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt      <  2:2831296/140‑1 (MQ=255)

                                                                                                                                  tATGCGCCG‑‑‑T‑T‑TTTTTCTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt      <  1:2220759/140‑1 (MQ=255)

                                                                                                                                     gcgcCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg   <  2:19475/140‑1 (MQ=255)

                                                                                                                                     gcgcCGT‑‑T‑T‑TTTTT‑TCTGTCACACCTTAATTACATCCCCATAGATTATTTGCGTCAGCTCACAACTACGCTTTTTCCCTGGTAAAAACTGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg   <  2:3041283/140‑1 (MQ=255)

                                                                                                                                     gcgcCG‑‑‑TGT‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTcgg   <  1:1145534/140‑3 (MQ=255)

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑‑T‑T‑TTTTT‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGC  >  NZ_CP025268/1374716‑1374988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`