BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,629,176	(T)_7→8	pseudogene (141/392 nt)	ydeI ←	YdeI family stress tolerance OB fold protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,629,169	1	.	T	96.5%	645.8 / 12.7	173	pseudogene (148/392 nt)	ydeI	YdeI family stress tolerance OB fold protein
Reads supporting (aligned to +/- strand): ref base . (0/6); new base T (45/122); total (45/128)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 175 total aligned reads displayed.

CCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAAT  >  NZ_CP025268/1629035‑1629301

ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑ttt‑t                                                                                                                                 >  1:1022823/1‑140 (MQ=255)

         tAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGcatca                                                                                                                         >  1:4677669/1‑140 (MQ=255)

         tAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TTT‑TTTGcatcat                                                                                                                        <  1:5090705/140‑1 (MQ=255)

          aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGcatcat                                                                                                                        <  1:754728/140‑1 (MQ=255)

          aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGcatcat                                                                                                                        <  1:3380632/140‑1 (MQ=255)

           aCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCatcatc                                                                                                                       >  1:25989/1‑140 (MQ=255)

                aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTc                                                                                                                  >  1:4203774/1‑140 (MQ=255)

                aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTGGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTc                                                                                                                  >  1:4204600/1‑140 (MQ=255)

                     tttCCCCGCCTTTATCCCCAACAACGTACCGTTCCTCTCCTTTCTGAGAAATCACATTACCGCGTAGTGAAACCGAAGCGCCCTCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGa                                                                                                             <  2:2210861/140‑1 (MQ=255)

                     tttCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGa                                                                                                             <  1:4344280/140‑1 (MQ=255)

                     tttCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGa                                                                                                             <  1:2246085/140‑1 (MQ=255)

                        cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAg                                                                                                          <  1:1803884/140‑1 (MQ=255)

                        cACCGCTCTTATCCCGAAAAACGTAACGGTCCTCTCCTTTGTGAGCAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGGATGGTTTTTGCGAAATCGACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAg                                                                                                          <  2:4501172/140‑1 (MQ=255)

                         aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAg                                                                                                          <  1:1840263/139‑1 (MQ=255)

                         aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAg                                                                                                          >  2:1840263/1‑139 (MQ=255)

                          ccGCTCTTATCGCGAAACACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:1345867/140‑1 (MQ=255)

                          ccGCTCTTATCGCGAAAAACGTCACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTCGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGcc                                                                                                        <  2:4095372/140‑1 (MQ=255)

                          ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCCTGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:4992318/140‑1 (MQ=255)

                          ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:4292412/140‑1 (MQ=255)

                          ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:1116523/140‑1 (MQ=255)

                          ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:2664594/140‑1 (MQ=255)

                          ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:187671/140‑1 (MQ=255)

                           cGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCa                                                                                                       <  1:4647878/140‑1 (MQ=255)

                           cGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCa                                                                                                       >  2:3827805/1‑140 (MQ=255)

                            gCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCAt                                                                                                      <  1:1556241/140‑1 (MQ=255)

                             ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                     >  1:4377043/1‑140 (MQ=255)

                             ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                     >  1:865071/1‑140 (MQ=255)

                                  aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                                <  1:5092833/140‑1 (MQ=255)

                                       gAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                           <  2:325511/140‑1 (MQ=255)

                                       gAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                           <  2:2251508/140‑1 (MQ=255)

                                          aaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGa                                                                                        <  2:2384284/140‑1 (MQ=255)

                                           aaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAg                                                                                       <  1:1696943/140‑1 (MQ=255)

                                             cGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc                                                                                     <  1:1454309/140‑1 (MQ=255)

                                                  cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg                                                                                <  1:4452253/140‑1 (MQ=255)

                                                    gTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAACTCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTg                                                                              <  1:297748/140‑1 (MQ=255)

                                                      ccTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgc                                                                            <  1:653665/140‑1 (MQ=255)

                                                      ccTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgc                                                                            <  1:4917070/140‑1 (MQ=255)

                                                       ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                           <  2:1555684/140‑1 (MQ=255)

                                                          tcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCAt                                                                                                      >  2:664576/1‑110 (MQ=255)

                                                              ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    <  1:4319811/140‑1 (MQ=255)

                                                              ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    <  2:2101383/140‑1 (MQ=255)

                                                              ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    >  1:312306/1‑140 (MQ=255)

                                                              ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    <  2:2547511/140‑1 (MQ=255)

                                                              ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    <  2:4941146/140‑1 (MQ=255)

                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGCAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   <  1:3382810/140‑1 (MQ=255)

                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                                <  2:2733144/111‑1 (MQ=255)

                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   >  2:2483451/1‑140 (MQ=255)

                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   >  1:687897/1‑140 (MQ=255)

                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   >  2:3377999/1‑140 (MQ=255)

                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   >  2:3835400/1‑140 (MQ=255)

                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   <  2:865008/140‑1 (MQ=255)

                                                                 tgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTg                                                                 >  2:3385343/1‑140 (MQ=255)

                                                                      aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                            >  2:3617378/1‑140 (MQ=255)

                                                                      aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                            >  1:3421123/1‑140 (MQ=255)

                                                                        tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                          <  2:3624017/140‑1 (MQ=255)

                                                                        tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTGTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATGTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                          <  2:4757294/140‑1 (MQ=255)

                                                                             ttACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGcc                                                     <  1:290131/140‑1 (MQ=255)

                                                                               aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                   <  2:2020059/140‑1 (MQ=255)

                                                                               aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TTTCTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                   <  2:3977618/140‑1 (MQ=255)

                                                                                   cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc                                               >  1:1047220/1‑140 (MQ=255)

                                                                                   cgTAGTGAAACCGAAGCGCCATCGTGCATGGATTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGCTTTAAACCGCCCTGGTc                                               >  2:2572045/1‑140 (MQ=255)

                                                                                    ttcgttaaaCCGATGCGCCATCGTTCATTGTTTCTGCGAAATCAACGCTCATT‑TTT‑TTTGCATCATCTGTTCCGCGATAGCCATCTTCTATTGCATGAGCCGGCGTTGGCGCTGCATCCTGTTTTAAACCGCCCAGGTCa                                              <  2:1799963/134‑1 (MQ=255)

                                                                                        tGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATcgg                                          >  1:3317802/1‑138 (MQ=255)

                                                                                         gAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGc                                         <  2:4362955/140‑1 (MQ=255)

                                                                                         gAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGc                                         <  1:2483451/140‑1 (MQ=255)

                                                                                         gAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGc                                         <  1:3043377/140‑1 (MQ=255)

                                                                                          aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCCACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                        <  1:5196438/140‑1 (MQ=255)

                                                                                          aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                        <  1:5196765/140‑1 (MQ=255)

                                                                                          aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                        <  1:845407/140‑1 (MQ=255)

                                                                                          aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                        <  2:3190023/140‑1 (MQ=255)

                                                                                           aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                       <  1:3248333/140‑1 (MQ=255)

                                                                                            aCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCaa                                      >  2:4377479/1‑140 (MQ=255)

                                                                                            aCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCaa                                      >  1:2230848/1‑140 (MQ=255)

                                                                                            aCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCaa                                      <  2:1109269/140‑1 (MQ=255)

                                                                                                aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                  >  1:2170794/1‑140 (MQ=255)

                                                                                                aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGGTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAATCCGCCCTGGTCATCTGCCAACGCa                                  <  2:1185815/140‑1 (MQ=255)

                                                                                                aaGCGCCATCGTGCATGGCTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATGGGTTCCGCGATAGGCATCTTCTATTGCATGAGGCGGCTGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                  >  2:2212967/1‑140 (MQ=255)

                                                                                                 aGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCAt                                 <  1:3174258/140‑1 (MQ=255)

                                                                                                  gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                                >  1:3663714/1‑140 (MQ=255)

                                                                                                     ccATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAgg                             <  1:3431324/140‑1 (MQ=255)

                                                                                                        tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAg                                                                                                          <  2:750285/60‑1 (MQ=255)

                                                                                                        tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                          <  1:4204581/140‑1 (MQ=255)

                                                                                                        tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                          <  1:1450889/140‑1 (MQ=255)

                                                                                                                 gTTTTTGCGAAATCAACGGTCA‑T‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  2:2608161/140‑1 (MQ=255)

                                                                                                                  tttttGCGAAATCAACTGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  1:449872/140‑1 (MQ=255)

                                                                                                                  tttttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCGATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  1:4787253/140‑1 (MQ=255)

                                                                                                                  tttttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  1:853996/140‑1 (MQ=255)

                                                                                                                  tttttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  2:3509180/140‑1 (MQ=255)

                                                                                                                  tttttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  2:2747562/140‑1 (MQ=255)

                                                                                                                  tttttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  1:2938342/140‑1 (MQ=255)

                                                                                                                  tttttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  1:3844170/140‑1 (MQ=255)

                                                                                                                  tttttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  2:2630050/140‑1 (MQ=255)

                                                                                                                    tttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACt              <  2:4193896/140‑1 (MQ=255)

                                                                                                                    tttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACt              <  1:2374850/140‑1 (MQ=255)

                                                                                                                     ttGCGAAATCAACGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                                <  1:2414660/105‑1 (MQ=255)

                                                                                                                         gAAATCCACGGTCA‑TGTTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAACCCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTa         <  1:2195214/140‑1 (MQ=255)

                                                                                                                         gAAAGCAACGGTCATT‑TTT‑TTTGAATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTCCATCCTGTCTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTa         <  2:5023997/140‑1 (MQ=255)

                                                                                                                              ccaCGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACa    <  2:1271044/138‑1 (MQ=255)

                                                                                                                               aaCGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACaa   <  1:3603447/140‑1 (MQ=255)

                                                                                                                                aCGGTCATT‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAAt  <  1:503247/140‑1 (MQ=255)

CCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TTT‑TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAAT  >  NZ_CP025268/1629035‑1629301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`