BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,178	+C	coding (314/351 nt)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,174	1	.	C	95.0%	502.6 / 17.4	160	coding (318/351 nt)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base . (6/2); new base C (55/96); total (61/99)
Fisher's exact test for biased strand distribution p-value = 5.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                          >  1:2908221/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                          >  2:4292392/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                          >  1:3729874/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                          >  1:1938747/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                          >  2:1105164/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                          >  1:4921119/1‑140 (MQ=255)

 gAAAAAAGCCGGAGTGATCACAAAAAACGTCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATATAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTAGGCTCCTACGTGCG‑c‑c                                                                                                                                        >  2:2542776/1‑140 (MQ=255)

 gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                        >  1:395518/1‑140 (MQ=255)

 gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                        >  1:2306883/1‑140 (MQ=255)

     aaaGACGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTGTTGTCAGCTGACACTTCCTCTTATCTTATGGATAAAATGGAGTTCTGTTCCTACGTGCG‑c‑c‑ccca                                                                                                                                   <  2:3765930/140‑3 (MQ=255)

        gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑cccagcc                                                                                                                                <  2:3440635/140‑6 (MQ=255)

        gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑cccagcc                                                                                                                                <  1:4865269/140‑6 (MQ=255)

          cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCg                                                                                                                              <  1:4225296/140‑1 (MQ=255)

          cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCg                                                                                                                              <  1:295296/140‑1 (MQ=255)

            gAGTGATCAC‑AAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCGCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGcaa                                                                                                                           <  2:5170941/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGcaaca                                                                                                                         <  2:3260465/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTGTTGTCAGCTGACACGTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGcaaca                                                                                                                         <  1:4830124/140‑1 (MQ=255)

                 aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAAt                                                                                                                       >  1:1679745/1‑140 (MQ=255)

                   cacaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGa                                                                                                                     >  1:1528179/1‑140 (MQ=255)

                               cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGc                                                                                                         <  1:3116593/140‑1 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                                >  1:3195675/1‑140 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                                >  1:2249378/1‑140 (MQ=255)

                                         cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                               <  1:385592/140‑1 (MQ=255)

                                         cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                               <  2:2892505/140‑1 (MQ=255)

                                           cTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCa                                                                                             <  1:5212568/140‑1 (MQ=255)

                                            tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                            <  1:4680582/140‑1 (MQ=255)

                                             gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                           >  2:505805/1‑140 (MQ=255)

                                              tgATTGGTTGACACATTATAAAATAGAGTACCTTTTGTTAGCTGAGAGTTTGTCTTATTTTATTGATAAAGTGGAGTTATGTGCCGACGTGCT‑CAC‑CC‑AGCACGCAACAATGATCAACATGTCGCCAAACGCAATCAACg                                                                                          >  2:1435467/1‑140 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          <  2:3195675/140‑1 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          <  1:594612/140‑1 (MQ=255)

                                                aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                        >  2:743074/1‑140 (MQ=255)

                                                aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                        <  1:455129/140‑1 (MQ=255)

                                                 aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       <  1:3422533/140‑1 (MQ=255)

                                                 aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       <  1:2176212/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:4576796/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:1416402/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:1550783/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:371461/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATATTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:4629239/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCGCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTCCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:5019592/140‑1 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:4619803/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:3742890/1‑140 (MQ=255)

                                                          aaaaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                              >  2:1815505/1‑140 (MQ=255)

                                                                   aaTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAGTGGATTTAGGTTCCTACGTGCGCC‑C‑CC‑AGCCGGCAACAAGGAGCAACATGCCGCAAAGCGCAATCAACGCACCCGGCCAGTCATAAagag                                                                     >  2:636617/1‑140 (MQ=255)

                                                                    aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGAGTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGGGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                    <  2:362951/140‑1 (MQ=255)

                                                                    aTAGAAGACCTTTTGTCAGCTTACACTTCCTCTTATCTTATTTATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                    <  2:2328450/140‑1 (MQ=255)

                                                                         aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                               >  1:1180406/1‑140 (MQ=255)

                                                                         aTACCTTTTGTCAGCTGACACTGCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑C‑C‑CCGAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCGACGCACCCGTCCAGGCATAAAGAGTCAGtt                                                               >  1:1025385/1‑140 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACCATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:4934806/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  1:990372/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:936146/140‑1 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  2:4766031/1‑140 (MQ=255)

                                                                                  gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                                      >  2:2738536/1‑140 (MQ=255)

                                                                                       cTGCCACTTCCTCTTATCTTATTGATAAACTAGATTTATGTTCCTACGTGCG‑C‑CACC‑AGTCCGCAACAATGATCAACATGGCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAATTTCATGCCATCaaca                                                 >  2:2420498/1‑140 (MQ=255)

                                                                                           cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                             <  2:686598/140‑1 (MQ=255)

                                                                                            acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  2:559876/140‑1 (MQ=255)

                                                                                            acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  1:2685587/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATGAACATGCCGCAAGGCGCAATAAACGCACCCGTCCAGGCATAACGAGTCAGTTTCACGCGAGCCACAACGCGAAGc                                        >  2:432082/1‑140 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:4508724/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:2249378/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:620866/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:3203340/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:2949649/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:5176103/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTAATAAAATGGATTTATGTCCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCACCGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAACCACAACGCGCAGc                                        <  1:5025911/140‑1 (MQ=255)

                                                                                                ccTCTTATCTCATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAGCAATGATCAACATGCCGCAAAGCGCAATCAACGCTCCCGTCCAGTCATAAAGAGTGAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:3807140/140‑1 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  1:4260193/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  2:2534051/1‑140 (MQ=255)

                                                                                                       tcttacTGATAACATGGATTTATGTCCCAACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCa                                 <  2:4192866/140‑1 (MQ=255)

                                                                                                        cttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                                <  1:1333520/140‑1 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  2:1938747/140‑1 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAGAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:4416844/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  2:1490998/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:889370/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  2:616188/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGGCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACgg                             >  2:1545356/1‑139 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGCGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATTCACAACGCGCAGCCACATAAAAGc                             >  2:2692101/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                            >  1:4682610/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                            >  1:3594319/1‑140 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  2:1422810/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  1:4072614/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGGCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  1:3529541/140‑1 (MQ=255)

                                                                                                                tAAAATGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGc                        >  2:3088079/1‑140 (MQ=255)

                                                                                                                 aaaaTGGATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCCAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                       <  1:4338844/140‑1 (MQ=255)

                                                                                                                       gATTTATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATa                 <  2:319649/140‑1 (MQ=255)

                                                                                                                         tttATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa               >  1:3050546/1‑140 (MQ=255)

                                                                                                                         tttATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa               >  1:4010390/1‑140 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:2830677/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:281182/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  2:885296/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:1153387/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACCTGCGAC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCCACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:4148404/140‑1 (MQ=255)

                                                                                                                             tGTTCCTACGCGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTAAGTTTCACGCCATCCACAACGCGCAGCCACATCACCGCCGTGCAGACATAAACGcc           >  2:3959/1‑140 (MQ=255)

                                                                                                                                 cctACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCGCATCAACGCCGTGCGGACATAAACGccacca       >  1:2887215/3‑140 (MQ=255)

                                                                                                                                 cctACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  1:4742154/3‑140 (MQ=255)

                                                                                                                                 cctACGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  1:774007/3‑140 (MQ=255)

                                                                                                                                    aCGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGGACCCGTCCAGGCGTAAAGAGTTAGTTTCCCGCCTTCCACAACGCGCAGCCACATCAAGGCCGTGCAGACCTAAACGCCACCATac    >  2:2882186/1‑139 (MQ=255)

                                                                                                                                     cGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg   <  2:4044899/140‑1 (MQ=255)

                                                                                                                                     cGTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg   <  2:128826/140‑1 (MQ=255)

                                                                                                                                      gTGCGCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGc  <  2:2913188/140‑1 (MQ=255)

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`