BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,701,269	+A	intergenic (‑380/+10)	uidA ← / ← uidR	beta‑glucuronidase/DNA‑binding transcriptional regulator UidR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,701,265	1	.	A	94.0%	1021.1 / 48.7	284	intergenic (‑376/+14)	uidA/uidR	beta‑glucuronidase/DNA‑binding transcriptional regulator UidR
Reads supporting (aligned to +/- strand): ref base . (8/9); new base A (124/142); total (132/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

AGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑A‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTAAA  >  NZ_CP025268/1701126‑1701399

    tATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑a‑aaa                                                                                                                                    >  1:4186852/1‑140 (MQ=255)

       tGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑a‑aaaaga                                                                                                                                 >  2:103136/1‑137 (MQ=255)

         ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑A‑Aaa‑agaga                                                                                                                              <  1:2303776/140‑1 (MQ=255)

           agcgaggaaCTCTCATGACAGGGGGCGACGGTGAATATTTTGTGAGGGATTCTCGGAAGTCGGGAGGGAGAATTTTGGCACAGAGAAATTACGATATGAAATGCGGGCACAGTGATCACCAGCCCGCC‑G‑A‑AAa‑agagaga                                                                                                                            >  2:5002937/8‑140 (MQ=255)

           aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑A‑AAa‑agagaga                                                                                                                            >  1:2113632/1‑140 (MQ=255)

              gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGaa                                                                                                                           >  1:3774028/1‑140 (MQ=255)

              gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGaa                                                                                                                           >  2:1853843/1‑140 (MQ=255)

              gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGaa                                                                                                                           >  2:2503808/1‑140 (MQ=255)

              gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGaa                                                                                                                           >  2:3597776/1‑140 (MQ=255)

                   aCTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAgg                                                                                                                      <  1:3023375/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGTAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGAt                                                                                                                    <  1:4354280/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGAt                                                                                                                    <  2:196464/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGAt                                                                                                                    <  2:1322876/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGAt                                                                                                                    <  1:345705/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGAt                                                                                                                    <  1:4071008/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGAt                                                                                                                    <  1:4241786/140‑1 (MQ=255)

                     tctaaggACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATGTTTGAACAGAGAAATTACGATATGAAACGCGGGAAAAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGAt                                                                                                                    <  1:5045683/134‑1 (MQ=255)

                       tcATGACGTATGGCTACAGTAAATATTTTGGGGGGAATACTCCGAAGCCAGGAAGGATAATTTTAGCACAGGGAAATTACGATATGAAACGCGGGAACAGTTATCACCAGGCCGCC‑GAA‑AAAGAGAGAGGAGCAGGGTgg                                                                                                                  <  2:1190106/140‑2 (MQ=255)

                       tcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGc                                                                                                                  <  2:2611205/140‑1 (MQ=255)

                       tcATGACATATGGCTAAAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTGGTGCACAGAGAAATTACGATAGGAGACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGc                                                                                                                  <  2:911298/140‑1 (MQ=255)

                         aTGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCgg                                                                                                                >  2:2212355/1‑140 (MQ=255)

                         aTGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCgg                                                                                                                >  2:4012083/1‑140 (MQ=255)

                           gACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGGGAGAGAATCAGGATGCGGtt                                                                                                              <  1:3942174/140‑1 (MQ=255)

                              atatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAg                                                                                                           >  1:2777501/1‑140 (MQ=255)

                              atatGGCTACAGTGAAGATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAg                                                                                                           >  1:1139409/1‑140 (MQ=255)

                               tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGa                                                                                                          >  1:1888787/1‑140 (MQ=255)

                                 tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACATAGAAATTACGAGATGAGACGCGGGCACAGTTATCACCAGCCCGCCGG‑A‑AAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                        >  2:4267224/1‑140 (MQ=255)

                                 tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                        >  1:2966079/1‑140 (MQ=255)

                                 tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTGATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                        <  2:3811338/140‑1 (MQ=255)

                                 tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTCGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                        >  2:3280091/1‑140 (MQ=255)

                                   gCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATAcc                                                                                                      <  2:4545532/140‑1 (MQ=255)

                                   gCTAAAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATAcc                                                                                                      >  2:4778346/1‑140 (MQ=255)

                                       cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  2:2340438/1‑140 (MQ=255)

                                       cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  1:1710470/1‑140 (MQ=255)

                                       cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  1:4468237/1‑140 (MQ=255)

                                       cAGTGAATATTCTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGCTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  2:397363/1‑140 (MQ=255)

                                        aGTGAATATTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCCGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                                 >  1:4333908/1‑140 (MQ=255)

                                          tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGGAATGACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatc                                                                                               >  1:3394643/1‑140 (MQ=255)

                                            aaTATTTTGGGGGGAATTCTCGGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                             >  2:3063005/1‑140 (MQ=255)

                                            aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                             >  2:3722579/1‑140 (MQ=255)

                                            aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGATATCACCAGCCCGCCGG‑A‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                             >  2:2417624/1‑140 (MQ=255)

                                             acatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcatc                                                                                            <  2:4346264/138‑1 (MQ=255)

                                              tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                           <  2:1331644/140‑1 (MQ=255)

                                                ttttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcgcg                                                                                         <  2:1836671/140‑1 (MQ=255)

                                                 tttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcgcgc                                                                                        >  1:1327090/1‑140 (MQ=255)

                                                   tGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      <  2:873358/140‑1 (MQ=255)

                                                   tGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      >  1:1553118/1‑140 (MQ=255)

                                                   tGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      >  1:1513829/1‑140 (MQ=255)

                                                   gggggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      <  2:2914631/139‑1 (MQ=255)

                                                          aaTTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑ACAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTACTCCCt                                                                               >  1:3657159/1‑140 (MQ=255)

                                                                    aGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacga                                                                     >  2:1697654/1‑140 (MQ=255)

                                                                    aGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacga                                                                     >  1:3237106/1‑140 (MQ=255)

                                                                      ccGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacgacg                                                                   >  2:3454573/1‑140 (MQ=255)

                                                                         ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGCGCAAACGACGCtt                                                                >  1:2651551/1‑140 (MQ=255)

                                                                              gATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAcgc                                                           <  1:1252579/140‑1 (MQ=255)

                                                                                 aaTTTTTGCACAGAGCAATTACGATATGAAACGCGGCCACAGTTATTACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTGAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc                                                        <  2:1621348/140‑1 (MQ=255)

                                                                                 aaTTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc                                                        <  2:740464/140‑1 (MQ=255)

                                                                                 aaTTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc                                                        <  1:2839714/140‑1 (MQ=255)

                                                                                       tGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCt                                                  <  1:4301840/140‑1 (MQ=255)

                                                                                         cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                                <  1:3694814/140‑1 (MQ=255)

                                                                                             gagaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCt                                            >  2:3570576/1‑140 (MQ=255)

                                                                                             gagaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCt                                            >  2:3193023/1‑140 (MQ=255)

                                                                                              agaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCtt                                           <  2:2738292/140‑1 (MQ=255)

                                                                                                   ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  1:4734032/140‑1 (MQ=255)

                                                                                                   ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  1:352119/140‑1 (MQ=255)

                                                                                                   ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  2:3479105/140‑1 (MQ=255)

                                                                                                   ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  1:622687/140‑1 (MQ=255)

                                                                                                   ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  1:1453992/140‑1 (MQ=255)

                                                                                                   ttACGATAGGAACCTCGGGCACAGTTATCACCAGCCCGCC‑TAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATACCGCGTACTCCCTCAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  2:4729754/140‑1 (MQ=255)

                                                                                                     aCGCTATGACACTCGGGCACAGTGATCACCATCCCGCC‑GAA‑AAAGAGAGAGTATCATGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAGCCGACGCTTCACGCGCCAGGTCTCCCGCTTCGCTATc                                    <  2:1286876/140‑1 (MQ=255)

                                                                                                     aCGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                    <  2:1924226/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:1109206/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  1:4836553/1‑140 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:3123457/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:3641438/140‑1 (MQ=255)

                                                                                                             aaaCGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                            <  2:3833778/140‑1 (MQ=255)

                                                                                                             aaaCGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                            <  1:4408168/140‑1 (MQ=255)

                                                                                                             aaaCGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                            <  1:4929698/140‑1 (MQ=255)

                                                                                                             aaaCGCGGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                            <  2:3374288/140‑1 (MQ=255)

                                                                                                                  cgGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCg                       <  1:3528373/140‑1 (MQ=255)

                                                                                                                  cgGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCg                       <  2:4505682/140‑1 (MQ=255)

                                                                                                                  cgGGCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCg                       <  1:861085/140‑1 (MQ=255)

                                                                                                                   gggCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGGGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGCCTTACGCTTCGATATCGGCCAGTACACCGt                      >  2:4082243/1‑140 (MQ=255)

                                                                                                                   gggCACAGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                      <  1:5145403/140‑1 (MQ=255)

                                                                                                                    gtcacaGTTATCAGCAGCTCGCC‑GAA‑AAAGAGATAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGATGAAAAGACGCTGCACGCGCCAGGTCTTCAGCTTAGATATAGGCCAGTACACCGTa                     >  2:1568365/3‑140 (MQ=255)

                                                                                                                     gCACAGTTAGCACCAGCCCGCC‑GAA‑AAAGAGAGATAATCAGGATGCGGTGAAGATGCCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGGCTTCCGCTTCGATATCGGCCAATACACCGTAg                    >  2:1115214/1‑140 (MQ=255)

                                                                                                                      cacaGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAgg                   <  1:182132/140‑1 (MQ=255)

                                                                                                                        caGTTATCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTc                 <  1:4524057/140‑1 (MQ=255)

                                                                                                                              tCACCAGCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATcc           >  1:4111450/1‑140 (MQ=255)

                                                                                                                                    gCCCGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCTATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGt     <  1:3747009/140‑1 (MQ=255)

                                                                                                                                     cccGCC‑GAA‑AAAGAGCGAGAATCAGGATGCGGTTAAGATACCGACAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCACATCCAGCAGTa    >  2:1414436/1‑140 (MQ=255)

                                                                                                                                     cccGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                           <  2:2885390/53‑1 (MQ=255)

                                                                                                                                     cccGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTCGGTCAGATCCAGCAGTa    >  1:3094110/1‑140 (MQ=255)

                                                                                                                                     cccGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:818052/1‑140 (MQ=255)

                                                                                                                                     cccGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:1620657/1‑140 (MQ=255)

                                                                                                                                     cccGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  2:2998089/1‑140 (MQ=255)

                                                                                                                                     cccGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  2:1466526/1‑140 (MQ=255)

                                                                                                                                     cccGCC‑GAA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  2:2296215/1‑140 (MQ=255)

                                                                                                                                       cgcc‑gaA‑AAAGAGGGAGAATCAGGATTCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGGCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTaaa  >  1:241441/1‑140 (MQ=255)

                                                                                                                                       cgcc‑gaA‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTaaa  >  2:5201338/1‑140 (MQ=255)

AGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑A‑AAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTAAA  >  NZ_CP025268/1701126‑1701399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`