BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,855,625	+C	intergenic (+116/‑50)	sppA → / → ansA	signal peptide peptidase SppA/asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,855,621	1	.	C	95.5%	1063.6 / 39.4	330	intergenic (+112/‑54)	sppA/ansA	signal peptide peptidase SppA/asparaginase
Reads supporting (aligned to +/- strand): ref base . (6/9); new base C (115/200); total (121/209)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

TGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTGTC  >  NZ_CP025268/1855483‑1855757

      aCCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑‑c‑cc‑cctg                                                                                                                                   >  1:4408349/1‑137 (MQ=255)

         tGCGCCAACATGCGTTACGTCTTGTACTGAGTGGCCGACAGATCGTCAGCCCCATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  2:431169/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGCTCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  2:597577/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  1:1093268/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  1:1361914/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  1:3306470/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  1:2037208/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCCTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  2:2582719/140‑1 (MQ=255)

          gcgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTc                                                                                                                                <  1:4538857/140‑1 (MQ=255)

           cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCa                                                                                                                               >  1:2366344/1‑140 (MQ=255)

           cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGGCGACGAAGCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑CGC‑‑C‑CC‑CTGCCTCa                                                                                                                               >  2:3294541/1‑140 (MQ=255)

           cgcCAACATGCGGTAAGGCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCa                                                                                                                               >  2:5021510/1‑140 (MQ=255)

                aCATGCGTGAAGTCTTGTACTGAGTGGCCGACAGATGGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATATTATCAGTTGCT‑C‑TC‑C‑CC‑CTGCCTCACGtat                                                                                                                          >  2:1292652/1‑140 (MQ=255)

                 cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGtata                                                                                                                         >  1:663665/1‑140 (MQ=255)

                 cATGCGTTAAGTCTTGTACTGACTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTGCCCGCTGTGTCGCCCCATATTTCCTTAGCCGCATAGTATCAGGTGCGAG‑C‑‑C‑CC‑CTAGCTCACGtata                                                                                                                         <  2:3701571/140‑1 (MQ=255)

                         aaGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTg                                                                                                                 <  1:2281113/140‑1 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCttt                                                                                                           <  1:2604362/140‑1 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCttt                                                                                                           <  2:2100792/140‑1 (MQ=255)

                                   cTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTGCCGCTGTTTCGCCCCATATTGCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTAGATACTTTTGCTCTTTCGat                                                                                                       <  1:928979/140‑1 (MQ=255)

                                    tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                      <  1:805820/140‑1 (MQ=255)

                                    tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                      <  1:322302/140‑1 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTTCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:3659733/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCCCGTATATACTTTTGCTCTTTCGatata                                                                                                    >  2:345609/1‑139 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:3560607/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:1570952/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:752791/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:3206628/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGACGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCAGAGTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTCTTGCTGTTTAGATAtc                                                                                                    >  1:4819933/1‑140 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:1412149/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:467901/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:3752423/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:3423085/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  2:1636088/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:3130514/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:2131427/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:1741520/140‑1 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                               >  1:2420622/1‑140 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                               >  1:448576/1‑140 (MQ=255)

                                              aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat                                                                                            <  1:1756108/140‑1 (MQ=255)

                                              aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat                                                                                            <  1:1851316/140‑1 (MQ=255)

                                              aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat                                                                                            <  2:475820/140‑1 (MQ=255)

                                                aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCTATATCATTCatatca                                                                                          >  1:589758/1‑140 (MQ=255)

                                                     gtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 >  2:2922467/1‑96 (MQ=255)

                                                     gtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  1:2922467/96‑1 (MQ=255)

                                                        ggCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATg                                                                                  <  1:334128/140‑1 (MQ=255)

                                                                       tACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAtt                                                                   >  2:334259/1‑140 (MQ=255)

                                                                        aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 >  1:1632809/1‑109 (MQ=255)

                                                                        aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAttt                                                                  >  1:5168393/1‑140 (MQ=255)

                                                                        aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAttt                                                                  >  1:4213529/1‑140 (MQ=255)

                                                                          gTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCCC‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTa                                                                 >  2:2275358/1‑140 (MQ=255)

                                                                          gTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTAc                                                                <  2:2743286/140‑1 (MQ=255)

                                                                          gTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCACAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTAc                                                                <  2:3315161/140‑1 (MQ=255)

                                                                             gacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATACCATGCAAAAGAAATCAATTTACGtt                                                             <  2:4663067/140‑1 (MQ=255)

                                                                             gaagaAACCTCTTACCGCTGTCTCGCGCCATATTTCCTTATCCGAATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGtt                                                             <  2:2560952/137‑1 (MQ=255)

                                                                               cgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGc                                                           <  2:4416712/140‑1 (MQ=255)

                                                                                   tCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTac                                                       <  1:134172/140‑1 (MQ=255)

                                                                                        ttCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATTTCATGCAAATGAAAGCAATTTACGTTGCCTACAcggg                                                  >  2:4215051/1‑140 (MQ=255)

                                                                                            cGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcggg                                              <  2:4490488/140‑1 (MQ=255)

                                                                                            cGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcggg                                              <  2:4533759/140‑1 (MQ=255)

                                                                                              cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGAc                                            <  2:2534545/140‑1 (MQ=255)

                                                                                              cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGAc                                            <  2:2372586/140‑1 (MQ=255)

                                                                                                gTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCa                                          <  1:4913551/140‑1 (MQ=255)

                                                                                                 tttCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCAt                                         >  2:2038162/1‑140 (MQ=255)

                                                                                                 tttCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCAt                                         <  2:1370432/140‑1 (MQ=255)

                                                                                                          atatTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCa                                <  2:4895002/140‑1 (MQ=255)

                                                                                                          atatTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCa                                <  1:5154907/140‑1 (MQ=255)

                                                                                                           tatTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCt                                                                                                                                 <  1:2771653/42‑1 (MQ=255)

                                                                                                               tCCTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGtt                           <  2:539572/140‑1 (MQ=255)

                                                                                                                ccTTATCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTc                          >  1:2688951/1‑140 (MQ=255)

                                                                                                                    gtCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACATATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  2:448576/139‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  1:1006816/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  2:826440/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  2:5156959/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  1:1839574/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  2:2306493/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      >  2:1227521/1‑140 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  2:1481437/140‑1 (MQ=255)

                                                                                                                     tCCGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGAGACCATCGGGATGCAGCGTTCCGAGc                     >  2:1042605/1‑140 (MQ=255)

                                                                                                                      tcGCATAGTAGCAGGTGCG‑C‑T‑‑C‑CCTCTTCCTCACGTATGTACTTTTGCACTTTCGAGATCATTCATGTCAATATCATGCAAAATAAATCATTTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGGTCCGAGCa                    <  2:2223187/139‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGTTGCG‑C‑TC‑C‑CC‑CTGCATCAAGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGAGGCAGCGTTCCGAGCa                    <  2:4731846/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:2953995/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  1:3326623/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:1849101/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:3405032/140‑1 (MQ=255)

                                                                                                                      acGCATAGTCTCTGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACGTTTGCGCTGTCGATATCATTCATATCAATATCTTTCCAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:179065/139‑1 (MQ=255)

                                                                                                                       cGCATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAg                   >  1:1618114/1‑140 (MQ=255)

                                                                                                                         cATAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAggg                 >  2:3837498/1‑140 (MQ=255)

                                                                                                                          aTAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    >  1:1952231/1‑136 (MQ=255)

                                                                                                                          aTAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:1952231/136‑1 (MQ=255)

                                                                                                                          aTAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGt                >  1:1225728/1‑140 (MQ=255)

                                                                                                                          aTAGTATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGt                >  2:2448678/1‑140 (MQ=255)

                                                                                                                              tATCAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtata            <  2:3930513/140‑1 (MQ=255)

                                                                                                                                tCAGGTGCG‑C‑T‑‑CTCC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:4084970/130‑1 (MQ=255)

                                                                                                                                 cAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc         <  1:3837498/140‑1 (MQ=255)

                                                                                                                                 cAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc         <  1:4577787/140‑1 (MQ=255)

                                                                                                                                 cAGGTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc         <  2:3088016/140‑1 (MQ=255)

                                                                                                                                   ggTGCG‑C‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCg       >  2:1093920/1‑140 (MQ=255)

                                                                                                                                      gcg‑c‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGTACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg    >  2:774742/1‑140 (MQ=255)

                                                                                                                                      gcg‑c‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg    >  1:4073349/1‑140 (MQ=255)

                                                                                                                                      gcg‑c‑TC‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg    >  1:1560397/1‑140 (MQ=255)

TGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑‑C‑CC‑CTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTGTC  >  NZ_CP025268/1855483‑1855757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`