BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	97.2%	1152.3 / 18.9	321	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (5/4); new base T (190/119); total (198/123)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 360 total aligned reads displayed.

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

cAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAATCATGGTGAATCGCCAGCg                                                                                                                                         >  2:3717198/1‑140 (MQ=255)

cAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAGTGACCGGAGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGACAGCg                                                                                                                                         <  1:4626683/140‑1 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttt                                                                                                                                  >  1:1078783/1‑140 (MQ=255)

      cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttt                                                                                                                                 >  1:1683683/1‑139 (MQ=255)

       aGCGAACCGTGGGCCTGCGAAGCGGCAACACAGGCGTAGCATACCGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                <  2:2075712/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                >  1:5077516/1‑138 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                <  2:4823859/140‑3 (MQ=255)

       aGCGAACAGTGGGCCGGCGAAGCTGCAACACAGGCGTAGCATACCGTTCGCACACGTAGTACGGGGCGCATATCACCTCGTTTATTGACAGGCGTCTGAACGGCAACAACCAAATCATGGTGAATCGCCAGCggctttta                                                                                                                                  <  2:4978648/140‑8 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              <  2:3936468/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              <  1:1758488/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              <  1:1500975/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              <  2:911842/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTGGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              <  1:4557481/140‑5 (MQ=255)

             ccGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGt                                                                                                                           >  1:1873898/1‑140 (MQ=255)

                tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTtat                                                                                                                        >  2:3250136/1‑140 (MQ=255)

                tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTtat                                                                                                                        >  1:3154427/1‑140 (MQ=255)

                 gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCCGCGTTT‑TTTACGCCGTtata                                                                                                                       >  1:2339027/1‑140 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgc                                                                                                                   >  2:362401/1‑140 (MQ=255)

                        agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCa                                                                                                                >  2:4862330/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTa                                                                                                           >  1:4905235/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTa                                                                                                           >  1:1072991/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATAGGACCTGGTGTATTGACCGGGGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTa                                                                                                           >  2:97546/1‑140 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                         >  2:1941000/1‑140 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                         >  1:2572027/1‑140 (MQ=255)

                                   cacaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGt                                                                                                     <  1:3255044/140‑1 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   <  1:516585/140‑1 (MQ=255)

                                      aGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGa                                                                                                  <  2:939314/140‑1 (MQ=255)

                                           tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  1:4191609/140‑1 (MQ=255)

                                           tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             >  1:4122266/1‑140 (MQ=255)

                                           tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATGGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  2:4439163/140‑1 (MQ=255)

                                              cATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAg                                                                                          >  1:3949292/1‑140 (MQ=255)

                                               aTACAGTTGGCAAATGTCGTACGGGGGGCATATGACCTGGTTTATTGCCCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCAGTTATAAAGCGCAACTTAAACAGTCGATCTCCGAgg                                                                                         <  2:1904706/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  2:4122266/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:1269326/140‑1 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                        <  2:5077516/140‑1 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  1:422430/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  1:4985905/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  2:3381674/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  2:3058584/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGATCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGAGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:436259/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgcgc                                                                                                                 <  1:3914557/108‑1 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  1:864682/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTGTTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCACCTTAAACAGTCGATCTCGGAGGCGATgaata                                                                                >  2:3558559/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         >  2:2940750/1‑131 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:2940750/131‑1 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  1:2169618/1‑140 (MQ=255)

                                                             tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAGCAGTCGATCTCCGa                                                                                           >  2:77978/1‑124 (MQ=255)

                                                             tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                           >  1:4013321/1‑140 (MQ=255)

                                                              gtagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGtt                                                                          <  1:3039383/140‑1 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  1:3502906/1‑140 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  2:1326687/1‑140 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  1:432666/1‑140 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtg                                                                     >  1:2256396/1‑140 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    >  1:4816250/1‑140 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    >  2:2720147/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCGAGCGTTT‑TTTACGCCGATATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGAGTTCGGtaactg                                                                 >  2:2279647/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:2447000/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:1208677/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:4245257/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:4760770/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:1772271/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAGAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  1:4432586/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTCAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  2:349647/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  2:3411847/1‑140 (MQ=255)

                                                                             tatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCg                                                           >  1:731825/1‑140 (MQ=255)

                                                                                gACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACa                                                        <  1:3350449/140‑1 (MQ=255)

                                                                                   cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  2:3188089/104‑1 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgc                                                                                                                   >  1:4846155/1‑76 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  1:3826323/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   <  2:163495/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:1554176/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAAAAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  2:631475/1‑140 (MQ=255)

                                                                                         tATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAAt                                               >  1:4388538/1‑140 (MQ=255)

                                                                                           ttGACCGGAGTCTGAACGTCAAAAACAAAAGCATGGTAAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAACCGTTCGGTGACTGTCGTCGACAGCGGCGAATcc                                             <  2:1170026/140‑1 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:1933449/130‑1 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  1:1656579/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:2163671/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:4718155/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:401943/1‑140 (MQ=255)

                                                                                                  gCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCa                                      <  2:2406658/140‑1 (MQ=255)

                                                                                                   cGTCTGAACGGCAAAAACAAAAGCATGGTGCATCGCCAGCG‑TTCTTTCCGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     <  2:147391/140‑1 (MQ=255)

                                                                                                   cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     <  2:693402/140‑1 (MQ=255)

                                                                                                        gAACGGCATAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCCACAGCGGCGAATCCGTATCCATTCCCa                                <  2:3805321/140‑1 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  2:1873898/140‑1 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  1:2392615/140‑1 (MQ=255)

                                                                                                         aaCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCAc                               >  2:4654115/1‑140 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                            <  1:4731611/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                            <  1:2641538/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                            <  2:432666/140‑1 (MQ=255)

                                                                                                              cAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATGAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                          >  2:4523035/1‑140 (MQ=255)

                                                                                                              cAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                          >  2:1225315/1‑140 (MQ=255)

                                                                                                                       aaGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCg                 >  1:3725185/1‑140 (MQ=255)

                                                                                                                            tGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCg            >  1:3773721/1‑140 (MQ=255)

                                                                                                                            tGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCg            >  1:669627/1‑140 (MQ=255)

                                                                                                                             ggTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa           >  1:3857646/1‑140 (MQ=255)

                                                                                                                                gAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  2:569619/140‑1 (MQ=255)

                                                                                                                                gAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  1:3520752/140‑1 (MQ=255)

                                                                                                                                  aTCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGttt      >  2:1003941/1‑140 (MQ=255)

                                                                                                                                  aTCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGttt      <  1:344/140‑1 (MQ=255)

                                                                                                                                     gCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcat   >  2:717552/1‑140 (MQ=255)

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`