BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,033,768	(C)_5→6	pseudogene (190/920 nt)	yedA →	drug/metabolite exporter YedA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,033,763	1	.	C	94.7%	843.6 / 37.6	266	pseudogene (185/920 nt)	yedA	drug/metabolite exporter YedA
Reads supporting (aligned to +/- strand): ref base . (9/5); new base C (132/118); total (143/123)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

TTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑T‑C‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NZ_CP025268/2033624‑2033897

ttGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑t                                                                                                                                           >  1:2495451/1‑140 (MQ=255)

     tctCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑T‑c‑cc‑cc                                                                                                                                   <  2:1006222/138‑1 (MQ=255)

        cATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑T‑c‑cc‑cccgc                                                                                                                                >  2:3404882/1‑137 (MQ=255)

         aTTCGGGGCTCCACCTATTTTGTCCATCGGATTGGCGTGCAAAGCTGGCCTCCGTTAATCACGGCGGCCTTTCCATTCCTGGCAGCCGGTATTTTATTGCTGGCATTATTGCTACTGCGGGGACACAAA‑C‑T‑c‑cc‑cccgct                                                                                                                               <  2:1418982/140‑5 (MQ=255)

           ttGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTAc                                                                                                                              <  2:3907745/140‑1 (MQ=255)

           ttGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTAc                                                                                                                              <  1:326544/140‑1 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGCTTCCTGGCACCCGGTATTCTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  2:4254992/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACgg                                                                                                                            >  1:4609122/1‑139 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:2803669/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:5111409/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:2902900/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:3752715/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:3914179/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:538917/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  2:1540335/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  2:3592663/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  2:4329867/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  2:4805866/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  2:4832770/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:2751327/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:2051020/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCAGTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  2:4206654/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCAGTAATTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:3693904/1‑140 (MQ=255)

             ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGAGTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGt                                                                                                                            >  1:1448814/1‑140 (MQ=255)

             ggggCGCAACCTATTTTGTCATTCGGATTGGCGTGGAAATCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACgg                                                                                                                            >  2:1872757/1‑139 (MQ=255)

               ggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTcc                                                                                                                          >  2:3634019/1‑140 (MQ=255)

                gCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCg                                                                                                                         <  1:2208976/140‑1 (MQ=255)

                gCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCg                                                                                                                         <  2:135110/140‑1 (MQ=255)

                gCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGCTATTTTATTGCTGGCATTTTTGCTACTGCGCGCACACAAA‑C‑TCC‑CC‑CCGCTACGTCCg                                                                                                                         <  2:2768569/140‑1 (MQ=255)

                  tCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCgct                                                                                                                       >  1:1223904/1‑140 (MQ=255)

                    aaCCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCgctgc                                                                                                                     >  1:4897886/1‑140 (MQ=255)

                         aTTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCATCCGGTATTGTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAAt                                                                                                                >  2:4594497/1‑140 (MQ=255)

                            ttGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGcc                                                                                                             >  1:1042660/1‑140 (MQ=255)

                             tGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCcg                                                                                                            <  1:1335530/140‑1 (MQ=255)

                              gTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACCCAAA‑C‑T‑C‑CCACCGCTACGTCCGCTGCTCAATGCcgc                                                                                                           >  2:3984014/1‑140 (MQ=255)

                              gTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCcgc                                                                                                           <  1:4454975/140‑1 (MQ=255)

                               tCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCcgcg                                                                                                          >  1:2600934/1‑140 (MQ=255)

                                  ttCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTg                                                                                                       >  1:2360669/1‑140 (MQ=255)

                                   tCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGa                                                                                                      <  1:3583827/140‑1 (MQ=255)

                                    cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCg                                                                                                                         >  2:4080216/1‑120 (MQ=255)

                                    cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCg                                                                                                                         >  2:3759789/1‑120 (MQ=255)

                                    cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                     <  1:1534373/140‑1 (MQ=255)

                                    cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                     <  2:2467289/140‑1 (MQ=255)

                                        ttGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 <  2:3265599/140‑1 (MQ=255)

                                        ttGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 <  1:4360901/140‑1 (MQ=255)

                                              tGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTa                                                                                           <  1:3740802/140‑1 (MQ=255)

                                                gAAAGCTGGCCTCCGTTAATGATGGCGGGGGGTCGATTCCTGGCAGCCGGTAGTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑CGC‑C‑CC‑CCGCTACGTCCGCTGGTCAATGCCGCGCTGGTTGGCCTGTTCtt                                                                                         >  2:2390444/1‑140 (MQ=255)

                                                     cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCg                                                                                                                         <  1:1727664/103‑1 (MQ=255)

                                                     cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    >  2:4913491/1‑140 (MQ=255)

                                                         ccTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                <  2:4652819/140‑1 (MQ=255)

                                                         ccTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                <  2:1103282/140‑1 (MQ=255)

                                                               ttAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTaa                                                                          <  2:2660137/140‑1 (MQ=255)

                                                                   tgatgGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGc                                                                      <  1:3667058/140‑1 (MQ=255)

                                                                      tgGCGGGCGTTCGATTCCTGGCGGCCGGTATTTCCTTGCCGCCATTTTTGCCACTGCGGGCACCCAAACC‑C‑C‑CC‑CCGCTACGTCCCCTGCTCACGGCGGCGCCGATTGGCCTGGGATTGCCGGCTGTCGGGAGTggtacg                                                                   <  2:3716425/140‑5 (MQ=255)

                                                                       ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATgg                                                                  >  2:1733167/1‑140 (MQ=255)

                                                                       ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATgg                                                                  >  2:1532495/1‑140 (MQ=255)

                                                                        gcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGt                                                                 <  2:1651827/140‑1 (MQ=255)

                                                                         cgggcgTTCGATTGCTGGCAGCGGGTATTTTATTGCTGGCATTTTTGCTACTGCTCGGAGACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCACTGCCGCGCTGATTGGCCGGTTATTGCCGGCTGTCGGTAATGGCATGGTg                                                                >  2:243444/1‑140 (MQ=255)

                                                                         cgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTg                                                                >  1:182373/1‑140 (MQ=255)

                                                                             cgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACgg                                                            >  1:3450730/1‑140 (MQ=255)

                                                                                  gATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAC‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGcc                                                       <  2:1209942/140‑1 (MQ=255)

                                                                                   aTTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCg                                                      <  1:3535978/140‑1 (MQ=255)

                                                                                            aGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCaaa                                             <  1:4801631/140‑1 (MQ=255)

                                                                                              ccGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGGGCTGATTGGCCTGTTAGTGCTGGCTGTCGGTAATGGCATCGTGACGGTTGCCGAACATCAAAAt                                           >  2:454149/1‑140 (MQ=255)

                                                                                              ccGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAAt                                           >  2:817663/1‑140 (MQ=255)

                                                                                              ccGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAAt                                           >  1:3928693/1‑140 (MQ=255)

                                                                                              ccGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAAt                                           >  1:4733080/1‑140 (MQ=255)

                                                                                                 gTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAT‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGtt                                        <  1:4197808/140‑1 (MQ=255)

                                                                                                 gTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGtt                                        <  2:4441614/140‑1 (MQ=255)

                                                                                                            cTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             >  1:4276248/1‑140 (MQ=255)

                                                                                                            cTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  1:4145775/140‑1 (MQ=255)

                                                                                                            cTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  1:496450/140‑1 (MQ=255)

                                                                                                            cTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  1:1938010/140‑1 (MQ=255)

                                                                                                            cTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  1:3771937/140‑1 (MQ=255)

                                                                                                              ggCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           <  2:3728373/140‑1 (MQ=255)

                                                                                                              ggCATTTTTGCTACTGCGAGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGGTTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           <  1:64303/140‑1 (MQ=255)

                                                                                                               gCATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcgc                          >  2:861009/1‑140 (MQ=255)

                                                                                                                cATTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcgcc                         <  1:4050431/140‑1 (MQ=255)

                                                                                                                 aTTTTTGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcgccg                        >  1:1250513/1‑140 (MQ=255)

                                                                                                                      tGCTACTGCGCGGACACAAC‑C‑G‑CACC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACNGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  1:42303/140‑1 (MQ=255)

                                                                                                                      tGCTACTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  2:5108348/140‑1 (MQ=255)

                                                                                                                      tGCTACTCAGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGATGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  2:4189321/140‑1 (MQ=255)

                                                                                                                       gctgctGCGCCGCCCCAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAAGGGCATGGTGACGGGTGCCGAACATCAAACGGTTCCTTCCGGCATCGCCGCCGTAg                  <  2:2937367/140‑1 (MQ=255)

                                                                                                                           cggcgcGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGt              <  1:4329867/138‑1 (MQ=255)

                                                                                                                           cTGCGCGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGt              >  1:827442/1‑140 (MQ=255)

                                                                                                                             gcgcGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGc                                                                      >  2:2531581/1‑82 (MQ=255)

                                                                                                                             gcgcGGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGc                                                                      <  1:2531581/82‑1 (MQ=255)

                                                                                                                              cgcgGACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGc           >  1:1368058/1‑140 (MQ=255)

                                                                                                                              cgcgCACACAAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCCCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGc           >  2:3341699/1‑140 (MQ=255)

                                                                                                                                     acaAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    <  1:5086343/140‑1 (MQ=255)

                                                                                                                                     acaAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    <  1:1146027/140‑1 (MQ=255)

                                                                                                                                      caAA‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGGAGTGGTTGCAACCGTGc   <  1:2434338/140‑1 (MQ=255)

                                                                                                                                       aaa‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:627311/140‑1 (MQ=255)

                                                                                                                                       aaa‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:932426/140‑1 (MQ=255)

                                                                                                                                       aaa‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:2955251/140‑1 (MQ=255)

                                                                                                                                       aaa‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:2303283/140‑1 (MQ=255)

                                                                                                                                       aaa‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:2243502/140‑1 (MQ=255)

                                                                                                                                       aaa‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:927618/140‑1 (MQ=255)

                                                                                                                                       aaa‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:803506/140‑1 (MQ=255)

                                                                                                                                       aaa‑C‑TCC‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:1477392/140‑1 (MQ=255)

TTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAA‑C‑T‑C‑CC‑CCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NZ_CP025268/2033624‑2033897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`