BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,292,596	(C)_6→7	pseudogene (361/2531 nt)	yejO ←	autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,292,590	1	.	C	96.8%	824.1 / 16.1	253	pseudogene (367/2531 nt)	yejO	autotransporter YejO
Reads supporting (aligned to +/- strand): ref base . (4/4); new base C (90/155); total (94/159)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑G‑C‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

aCCGCGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑g‑g                                                                                                                                           >  2:5139365/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑g‑g                                                                                                                                           >  2:4726673/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑g‑g                                                                                                                                           >  2:1694185/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑g‑g                                                                                                                                           <  1:2783442/140‑1 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑g‑g                                                                                                                                           >  1:240196/1‑140 (MQ=255)

     gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAACGAC‑G‑T‑c‑c‑ccc                                                                                                                                   >  2:3611903/1‑140 (MQ=255)

     gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑G‑c‑c‑ccc                                                                                                                                   <  2:1445400/140‑1 (MQ=255)

      aCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑G‑c‑c‑ccc‑c                                                                                                                                 >  2:2587819/1‑140 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑G‑c‑c‑ccc‑ccatc                                                                                                                             >  1:4719846/1‑136 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑G‑c‑c‑ccc‑ccatc                                                                                                                             >  1:676402/1‑136 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAAc                                                                                                                     >  1:628355/1‑140 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTa                                                                                                                >  1:3778322/1‑140 (MQ=255)

                          ccATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAg                                                                                                              >  1:2203828/1‑140 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAgcgc                                                                                                           >  1:4432088/1‑140 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTACCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGcgccc                                                                                                         <  2:1418106/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGcgccc                                                                                                         <  1:151114/140‑1 (MQ=255)

                                cccGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAggtgccg                                                                                                        >  1:2665521/1‑134 (MQ=255)

                                cccGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGcgcccg                                                                                                        >  1:3966535/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  1:5086777/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:2296699/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:453931/1‑140 (MQ=255)

                                     gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCttt                                                                                                   >  2:1148616/1‑140 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  1:876460/140‑1 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  1:3386797/140‑1 (MQ=255)

                                          tgttTCACTAGCACCTGCTGCCGTCTGATTTACCGCAGATGCAGCTCCCCCCTCTTTAACCTCCAGTAGTCCTATCTTTTTGGTAGCGGAAAAGAC‑GTC‑C‑C‑CCC‑CTTCTGTTTCAACGATTATGCGCCCGCCTTTAATCg                                                                                              <  2:3464842/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  1:245689/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  2:1932608/140‑1 (MQ=255)

                                                     cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                                                                   >  2:4234737/1‑140 (MQ=255)

                                                       ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCGCCCTCTTTAACCTCCCATAGACCGCCCTTTTCGATAGCGACAAATAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCgg                                                                                 >  1:4607556/1‑139 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAACTCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:1787962/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:3046194/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:274151/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:2076846/140‑1 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGCCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:1967585/1‑140 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          <  1:1883117/140‑1 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          <  1:2573878/140‑1 (MQ=255)

                                                                    gATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTGCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGAATCATTCGCTGCGCCATAAAcc                                                                    >  2:4089579/1‑140 (MQ=255)

                                                                        tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTa                                                                >  1:1032244/1‑140 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:3029692/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:3871301/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:1893569/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:1777137/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:1202939/140‑1 (MQ=255)

                                                                           cGCAAAGGCAAATCCCCCCTCTGTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGGGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:2165155/140‑1 (MQ=255)

                                                                             caaatgcacctCCCCCCTCTTTAACCTCCAGGAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                           <  2:4707671/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCCACACAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTACGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                           <  1:3703325/140‑1 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  1:3360642/140‑1 (MQ=255)

                                                                                       tCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                                 <  1:4039759/140‑1 (MQ=255)

                                                                                        ccccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAg                                                <  1:1267030/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTATTCCTCCCTTTTCGCTAGCGACAAAGAC‑G‑CCC‑C‑CCC‑CATCTTCTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                              <  2:4156740/140‑1 (MQ=255)

                                                                                          ccccTCTGTAACCTCCATTAGTCCTCCCTTTTCGATAGCGACAAAGACAC‑C‑C‑C‑CCC‑CATCTTTTTCAACGATTAAGGGCCGGCCTTTAGTCTTGGTATCATTCGCTGCGTGATTAACCTGTGGCAGACCCTTATTGAGCg                                              <  2:971658/140‑1 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGTTAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCATATTCAACGATTAAGCGCCCGCCATTAATCGGGATATCATTCGCTGCGCAATACACGTGTAGCACACAAATATTGAGccaa                                            >  2:390333/1‑137 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa                                            >  1:1081097/1‑140 (MQ=255)

                                                                                              tctTTACCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCCTAGt                                          <  2:4804710/140‑1 (MQ=255)

                                                                                                        cgagtagtCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTGTTGAGCGAAGTCTGGTTTGcc                                >  2:2545169/3‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  2:1814942/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  1:982970/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  1:464695/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  2:265547/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  1:4881927/1‑140 (MQ=255)

                                                                                                         cgggagtCCTCCCTTTGCGATAGCGACGGAGAC‑G‑GCC‑C‑CCC‑CAGCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAGTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               <  2:5213502/136‑1 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAT‑G‑G‑C‑C‑CCCGCATCTTTTTCATCGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               <  2:1987171/140‑1 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:3322420/1‑140 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:5040995/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             >  1:1676083/1‑109 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:2805393/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:3340634/1‑140 (MQ=255)

                                                                                                           gtagtCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgcg                             <  2:3026427/140‑1 (MQ=255)

                                                                                                           gtagtCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgcg                             <  2:2460353/140‑1 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  2:213984/140‑1 (MQ=255)

                                                                                                               tcctccCTTTTCGATAGGGACAAAGAC‑G‑G‑CGC‑TCC‑CATCTTTTGCAACGGTTAAGCGCCGGGCTGGAATCGTGGTATCATTCGCTGCGGCATAAACCTGTAGCACACCATGGTTGAGCGTAATATGGGTTGCCGCGGCat                         >  2:4427480/1‑139 (MQ=255)

                                                                                                               tcctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAc                         >  1:714193/1‑140 (MQ=255)

                                                                                                                cctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc                        <  1:2304358/140‑1 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       >  1:4271114/1‑92 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  2:4271114/92‑1 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:2698903/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:962528/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  2:5097704/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  2:5044495/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  2:2971473/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑G‑G‑C‑C‑CCCACATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATCATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  2:3719361/1‑140 (MQ=255)

                                                                                                                        ttCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                <  2:1841788/140‑1 (MQ=255)

                                                                                                                        ttCGATAGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                >  2:4465313/1‑140 (MQ=255)

                                                                                                                              aGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCTACCCATTTGAACAAttt          >  2:3154039/1‑140 (MQ=255)

                                                                                                                              aGCGACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAAttt          >  2:753146/1‑140 (MQ=255)

                                                                                                                                 gACAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGAt       <  2:4553647/140‑1 (MQ=255)

                                                                                                                                  aCAAAGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATa      >  1:1816972/1‑140 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTCATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACAGCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:4252222/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:667042/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:56224/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:1148616/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:3355698/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:1357968/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:3966535/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:3559466/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:5169900/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:2698903/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATGTGAACAATTTGATAACCg  <  2:3401143/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑GCC‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTGAATCGTCGTATCATTCGCTGCGCCGTAAACCTGTAGCACACCATTCTTGAGCGTAGTCTGGTTTGCCGCGTCAGCCATTTGAACAATTTGATAACCg  <  1:3111465/140‑1 (MQ=255)

                                                                                                                                      aGAC‑G‑G‑C‑CGCTC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAGTCGCTGCGCCATAAACCTGTAGCACACCATTATTTAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:3819582/140‑1 (MQ=255)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑G‑G‑C‑C‑CCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`