BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,858,752	+T	pseudogene (395/2078 nt)	flhA →	formate hydrogenlyase transcriptional activator FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,858,748	1	.	T	97.0%	995.8 / 17.2	270	pseudogene (391/2078 nt)	flhA	formate hydrogenlyase transcriptional activator FlhA
Reads supporting (aligned to +/- strand): ref base . (6/2); new base T (131/129); total (138/132)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

GCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑T‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858611‑2858882

 cagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCAGAAGGGCATA‑t‑t‑t                                                                                                                                     >  2:4464548/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACTCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  1:1158195/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTCCGAAGAATTTTGTGAAACCTCGCCGCAGCTGGACGCAGGTCGGCTATACCCAACATTTGGTCACTATTGCCGGACGCCACTGGTGGCGGGCGGGCAGA‑t‑t‑tt                                                                                                                                    >  2:1106847/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTGACTATTGCCTGATGCCTCTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  1:4772559/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  2:800430/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  1:699035/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  2:4528600/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  1:1536096/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  2:4418223/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  1:2777065/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  2:326437/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  2:1398935/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tt                                                                                                                                    >  2:1543331/1‑140 (MQ=255)

    caTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tttg                                                                                                                                  >  2:1816400/1‑138 (MQ=255)

           tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCtg                                                                                                                            <  1:46313/140‑1 (MQ=255)

           tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCtg                                                                                                                            <  1:2609247/140‑1 (MQ=255)

              cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGa                                                                                                                         >  1:1812370/1‑140 (MQ=255)

                cccTGATACGCTGCATAGCAGTTACGAAGACTTTTGTGAAACCTGGCCGCGGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTAACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGaca                                                                                                                       >  2:1713130/1‑138 (MQ=255)

                   tGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTa                                                                                                                    <  2:2727109/140‑1 (MQ=255)

                    gacaCGCTGCATTGCAGTTACGAAGCATTTTGTGCAACCTGGCCGCAGCCGGGCGCAGGTGGGCTATACCCAAACTTTGGTCACTATTGCCCGATGCAACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTAt                                                                                                                   <  2:3822860/137‑1 (MQ=255)

                       aCGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCg                                                                                                                <  1:1000570/140‑1 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:4902714/1‑140 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:1082155/1‑140 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:3057136/1‑140 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tttgg                                                                                                                                 <  2:3472558/118‑4 (MQ=255)

                             cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:2570348/1‑140 (MQ=255)

                             cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:1334165/1‑140 (MQ=255)

                             cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:2958175/1‑140 (MQ=255)

                               ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        >  1:953706/1‑140 (MQ=255)

                               ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        <  1:3375831/140‑1 (MQ=255)

                               ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑t‑t‑tttgg                                                                                                                                 <  2:4660326/114‑4 (MQ=255)

                               ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGACACTGGCGGCGGAAGGGCATA‑t‑t‑tttgg                                                                                                                                 >  1:4660326/1‑111 (MQ=255)

                                 gccgTTACGAAGAATTTTGTGAGAGCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCCGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTAGTCGTTATGACGAt                                                                                                      <  2:2462700/137‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGCAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGCCATA‑TCT‑TTCGTGGCTGTGCATTTATTCGTTCTGACGAt                                                                                                      <  2:3043588/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCGTGATGCCACTGGCGGCTGCAGGGCATA‑T‑TGTTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:4766856/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:4777361/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:2418911/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:3553267/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:2251997/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:2887563/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:3649414/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:4362716/140‑1 (MQ=255)

                                     ttACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGACGTGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGcc                                                                                                  >  1:3029766/1‑140 (MQ=255)

                                       aCGCAGAATTTTGTGAAACATGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCAACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTAGTTATGACGATAGcccc                                                                                                >  2:743803/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACTCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  2:3997932/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:3201406/134‑1 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  1:480484/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  2:3733471/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  1:2317611/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGACGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTTAATTTATTCGTTATGACGATCGcccc                                                                                                >  1:3696411/1‑140 (MQ=255)

                                              aTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCg                                                                                         <  1:4759067/140‑1 (MQ=255)

                                                   gtgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaaa                                                                                    <  1:782697/140‑1 (MQ=255)

                                                    tgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAag                                                                                   >  1:266655/1‑140 (MQ=255)

                                                            ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt                                                                           <  2:4943208/140‑1 (MQ=255)

                                                                   gCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGc                                                                    <  1:2428008/140‑1 (MQ=255)

                                                                    cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  2:5076715/140‑1 (MQ=255)

                                                                       gACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAAc                                                                >  2:589399/1‑140 (MQ=255)

                                                                         cGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACAt                                                              <  1:4289245/140‑1 (MQ=255)

                                                                            aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAAGTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTa                                                           >  1:672302/1‑140 (MQ=255)

                                                                              gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         <  2:32198/140‑1 (MQ=255)

                                                                              gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         <  1:1561424/140‑1 (MQ=255)

                                                                              gTGGGCTATACCCAAAAGTTGGTCACTATTGCTTGATGACACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATGTAGTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         <  2:2368016/140‑1 (MQ=255)

                                                                                   cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                    <  1:593996/140‑1 (MQ=255)

                                                                                      taCTCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCGCCTGGAGGGAAAAAGAGTTCAATCGTCTGCACACATTTACGCAGATCGt                                                 >  2:2857441/1‑140 (MQ=255)

                                                                                      taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  2:5188959/1‑140 (MQ=255)

                                                                                      taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  1:4087786/1‑140 (MQ=255)

                                                                                      taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  1:1430298/1‑140 (MQ=255)

                                                                                      taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  2:3525055/1‑140 (MQ=255)

                                                                                        cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGCGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                               >  1:5031670/1‑140 (MQ=255)

                                                                                           aaaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGATTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTg                                            >  2:1220866/1‑140 (MQ=255)

                                                                                            aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           <  1:1476510/140‑1 (MQ=255)

                                                                                            aaaTTGGGTCACTATTTCCTGATGCCACTGCCGGCGGACGACCACACT‑T‑TTCGTCCCTGTGAATTTATTCGCTATGACGATCGCCCCTGGAGCGACACAAACTTCAACCGTCCGCACACGTTCACGCCGATCGCTTCTgc                                           >  2:2788327/1‑139 (MQ=255)

                                                                                             aaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtc                                          >  1:1474483/1‑140 (MQ=255)

                                                                                                  ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                     >  1:4342934/1‑140 (MQ=255)

                                                                                                  ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                     <  2:5002549/140‑1 (MQ=255)

                                                                                                  ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                     <  1:2830917/140‑1 (MQ=255)

                                                                                                   gTCAGTATTGCCTGATGCCACTGGCGGCGGCAGGGCATATT‑T‑TTGGTCGCTGTTAATTTATTCGTTATGACGATCGCCCCTGGCGCGAAAACAAGTTCAACTGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAcc                                    >  2:3146108/1‑140 (MQ=255)

                                                                                                   gTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAcc                                    >  1:4929601/1‑140 (MQ=255)

                                                                                                   gTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATAGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAcc                                    >  2:4871901/1‑140 (MQ=255)

                                                                                                       cTATTGCCTGATGGCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGCTCGCCCCTGGAGCGAAAAAGAGTTCCATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:64373/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:4501716/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:2175673/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:1712489/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGGCTGCAAACATTTACGCAGATCGTTTCGGTCGTCACCGAAc                                <  1:5086728/140‑1 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                    >  1:3866805/1‑109 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                    <  2:3866805/109‑1 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:3739349/1‑140 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:1999981/1‑140 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:3959418/1‑140 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:4053378/1‑140 (MQ=255)

                                                                                                                   gCCACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                    <  2:1475396/140‑1 (MQ=255)

                                                                                                                    ccACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCG‑AAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  >  1:5043719/1‑140 (MQ=255)

                                                                                                                     cACTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  2:297030/140‑1 (MQ=255)

                                                                                                                      aCTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCTTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgcg                 >  2:4227263/1‑140 (MQ=255)

                                                                                                                      aCTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgcg                 <  1:3492219/140‑1 (MQ=255)

                                                                                                                       cTGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgt                >  2:2577342/1‑140 (MQ=255)

                                                                                                                        tGGCGGCGGAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgtc               >  2:1516242/1‑140 (MQ=255)

                                                                                                                            ggcggAAGGGCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTa           <  1:4572380/140‑1 (MQ=255)

                                                                                                                                   gggCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGt    <  2:4196800/140‑1 (MQ=255)

                                                                                                                                    ggCATATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCACTCGTCTGCAAACATTTACGCAGAACGTTTCTGTCGTCACCGAACAAATACAGAGCCGCGTCGTTACCAATGTc   <  2:1947712/140‑1 (MQ=255)

GCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑T‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858611‑2858882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`