BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,862,734	+T	intergenic (+9/+41)	pphB → / ← ygbI	protein‑serine/threonine phosphatase/DNA‑binding transcriptional repressor YgbI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,862,733	1	.	T	99.4%	654.7 / NA	177	intergenic (+8/+42)	pphB/ygbI	protein‑serine/threonine phosphatase/DNA‑binding transcriptional repressor YgbI
Reads supporting (aligned to +/- strand): ref base . (1/0); major base T (101/74); minor base G (1/0); total (103/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

CAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGAT  >  NZ_CP025268/2862594‑2862866

caaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc                                                                                                                                        <  1:1184565/140‑1 (MQ=255)

caaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc                                                                                                                                        <  1:2737041/140‑1 (MQ=255)

caaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc                                                                                                                                        <  1:514442/140‑1 (MQ=255)

caaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc                                                                                                                                        <  1:4126997/140‑1 (MQ=255)

  aaTAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc‑tt                                                                                                                                     <  1:784002/140‑2 (MQ=255)

   aTAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTACCCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc‑ttc                                                                                                                                    >  1:3744435/1‑138 (MQ=255)

   aTAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc‑ttc                                                                                                                                    >  1:4564180/1‑138 (MQ=255)

   aTAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTAATAAAATAAAGTAGTCTCATGc‑ttc                                                                                                                                    >  2:4703175/1‑138 (MQ=255)

     aaaCGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc‑ttctt                                                                                                                                  <  1:2697976/140‑5 (MQ=255)

     aaaCGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc‑ttctt                                                                                                                                  <  1:2697493/140‑5 (MQ=255)

      aaCGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGc‑ttcttc                                                                                                                                 <  1:4504401/140‑6 (MQ=255)

        cGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCtg                                                                                                                                <  2:3375682/140‑1 (MQ=255)

          gcgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCtgtg                                                                                                                              <  2:3004916/140‑1 (MQ=255)

          gcgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCtgtg                                                                                                                              >  1:898095/1‑140 (MQ=255)

           cgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGa                                                                                                                             >  2:4828060/1‑140 (MQ=255)

           cgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGa                                                                                                                             >  1:2971454/1‑140 (MQ=255)

           cgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGa                                                                                                                             >  1:3454022/1‑140 (MQ=255)

           cgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGa                                                                                                                             >  1:5036394/1‑140 (MQ=255)

           cgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGa                                                                                                                             >  2:1493419/1‑140 (MQ=255)

           cgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGa                                                                                                                             >  2:2310332/1‑140 (MQ=255)

              tGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGc                                                                                                                          <  1:4796631/140‑1 (MQ=255)

              tGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGc                                                                                                                          <  1:1114658/140‑1 (MQ=255)

                attattTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCAt                                                                                                                        <  1:299630/140‑1 (MQ=255)

                attattTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCAt                                                                                                                        <  1:300039/140‑1 (MQ=255)

                  tattTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGa                                                                                                                      <  2:3453708/140‑1 (MQ=255)

                        atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAAcc                                                                                                                <  1:4597957/140‑1 (MQ=255)

                        atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAAcc                                                                                                                <  1:44190/140‑1 (MQ=255)

                        atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAAcc                                                                                                                <  2:2911950/140‑1 (MQ=255)

                        atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAAcc                                                                                                                <  1:1311626/140‑1 (MQ=255)

                        atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAAcc                                                                                                                <  1:3878310/140‑1 (MQ=255)

                        atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAAcc                                                                                                                <  1:3745362/140‑1 (MQ=255)

                         tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAAccc                                                                                                               <  2:513901/140‑1 (MQ=255)

                          atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATGCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGc                                                                                                                          >  2:1429119/1‑128 (MQ=255)

                              ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                          <  1:1493419/140‑1 (MQ=255)

                                   tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                                     >  1:4185625/1‑140 (MQ=255)

                                   tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGATAttt                                                                                                     >  2:4575180/1‑139 (MQ=255)

                                          tttGATAACATTCAGACGTTCGCTACCCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATACAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCa                                                                                              >  1:1982130/1‑140 (MQ=255)

                                          tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTTAAGCATGAGTAACCCGGTGTTATTGCCGGCCa                                                                                              >  2:4567855/1‑140 (MQ=255)

                                          tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCa                                                                                              >  1:138583/1‑140 (MQ=255)

                                          tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCa                                                                                              >  2:3743626/1‑140 (MQ=255)

                                          tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTCCGGGCCa                                                                                              >  2:151126/1‑140 (MQ=255)

                                            tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatt                                                                                            >  1:2502666/1‑140 (MQ=255)

                                            tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatt                                                                                            >  2:2197007/1‑140 (MQ=255)

                                            tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatt                                                                                            >  2:3206833/1‑140 (MQ=255)

                                            tGATAACATTCAGACGCTCGCTAACCAGATTTATATTGATACCGGATGGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAATTAGTCCCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCGatt                                                                                            >  2:1595026/1‑140 (MQ=255)

                                             gATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatta                                                                                           >  1:3234150/1‑140 (MQ=255)

                                               tAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCattatt                                                                                         <  1:2673766/140‑1 (MQ=255)

                                                    ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAttt                                                                                    >  2:1925946/1‑140 (MQ=255)

                                                    ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAttt                                                                                    >  2:4230751/1‑140 (MQ=255)

                                                    ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAttt                                                                                    >  2:1007731/1‑140 (MQ=255)

                                                            ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAc                                                                            >  2:1100548/1‑140 (MQ=255)

                                                            ttCGCTAACCAGATTTAGATTGATACCTGATCGCCTAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTCCTGTGAAGCATGAGTAACCCGGGGTTATTGCAGGCCAGTATTCATTTTTCGCTAc                                                                            >  2:1567013/1‑140 (MQ=255)

                                                             tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                                           >  1:1089885/1‑140 (MQ=255)

                                                             tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                                           >  1:4290182/1‑140 (MQ=255)

                                                             tCGCTAACCAGATTGATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCTGGTGTTATTGCAGGACATTATTCATTTTTCGCTGcc                                                                           >  2:1764855/1‑140 (MQ=255)

                                                              cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                          >  1:781162/1‑140 (MQ=255)

                                                              cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                          >  1:2241348/1‑140 (MQ=255)

                                                              cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCGTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                          >  2:2366078/1‑140 (MQ=255)

                                                                cTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGc                                                                        <  1:3673085/140‑1 (MQ=255)

                                                                     cAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTACCCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAaga                                                                   <  2:3307670/140‑1 (MQ=255)

                                                                     cAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAaga                                                                   <  1:1595747/140‑1 (MQ=255)

                                                                       gATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagaga                                                                 >  2:4193352/1‑140 (MQ=255)

                                                                        aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagagag                                                                >  1:3712786/1‑140 (MQ=255)

                                                                          ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                              <  1:3118645/140‑1 (MQ=255)

                                                                                  aTACCGGATCGCCGAACCTCGGGCGGCGGTCATTTTATAAAAAAAAGTACTCTCAGCCTTCATCGGTGAAGCAAGAGTAACCCGGTGTTATTGCCGGCAATTATCCATTTGTCGCTACCAACAAAGAAAGAACCTGCGTc                                                      >  2:2543284/1‑140 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCTGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  2:4665820/1‑140 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGGAGCATGAGCAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  2:4590442/1‑140 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  2:4145055/1‑140 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  1:4453883/1‑140 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  2:1679350/1‑140 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  2:4988903/1‑140 (MQ=255)

                                                                                  aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                      >  1:160551/1‑140 (MQ=255)

                                                                                  aTACCGGATCGCAGAACAGCGGTCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCGTCGTCTGTGAAGCATGAGTAACCCGGTGTGATTGCAGGGAATTAGTTATTTTTGGCTACCAGCAAAGAGAGATCCTACttt                                                      >  2:1891205/1‑139 (MQ=255)

                                                                                   tACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCa                                                     >  1:233628/1‑140 (MQ=255)

                                                                                      cGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTGTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCa                                                  >  2:409158/1‑140 (MQ=255)

                                                                                        gATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgc                                                >  2:3509088/1‑140 (MQ=255)

                                                                                         aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcg                                               <  1:4040197/140‑1 (MQ=255)

                                                                                          tCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgc                                              <  1:125384/140‑1 (MQ=255)

                                                                                           cGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCCTTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcg                                             >  1:209470/1‑140 (MQ=255)

                                                                                              cGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGa                                          <  1:1030186/140‑1 (MQ=255)

                                                                                                  cAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTgg                                      <  2:3134872/140‑1 (MQ=255)

                                                                                                    gcgggcggCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGCTCCTGCTTCACCAGCGCGCGACTGGCa                                    <  1:253259/140‑1 (MQ=255)

                                                                                                    gcggccggCTGCCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGGAACCCGGTGTTATTGCAGCCCATTATTCATTTTTCCCTACCAGCAAACAGAGATCCTGCTTCACCAGCGCGCCACTGGCa                                    <  2:3830375/140‑1 (MQ=255)

                                                                                                     cgggcggCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAc                                   >  1:4499594/1‑140 (MQ=255)

                                                                                                      gggcggCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAct                                  <  2:1089885/140‑1 (MQ=255)

                                                                                                      cggcggcTGTCATTTTCTAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAct                                  <  2:151844/139‑1 (MQ=255)

                                                                                                       ggcggcTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCActc                                 >  2:3613764/1‑140 (MQ=255)

                                                                                                             tGTCATTTTATAAGATAAAGTAGTCTCATGCTTCTTCTGTGAAGCCTGAGTAACCCGGTGCTATTGCAGGCCAGTATTCCTTTTTCGCTACCAGCAAAGACAGATCCTGCCCCACCATCGCGCGACTGGCACTCTCCCGc                           >  2:400098/1‑140 (MQ=255)

                                                                                                             tGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGc                           <  1:3532187/140‑1 (MQ=255)

                                                                                                               tCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCaa                         >  1:2300058/1‑140 (MQ=255)

                                                                                                                     tataAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtc                   <  1:1529631/140‑1 (MQ=255)

                                                                                                                       taaaataaaTTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCTGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcctcgt                 <  2:4498380/140‑1 (MQ=255)

                                                                                                                       taaaataaaGTAGTCTCATGCTTCTTTTGTGAAGCATGAGTACCCCGGTGTTATTGCAGGCCATTATTCCTTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt                 <  2:4430155/140‑1 (MQ=255)

                                                                                                                       taaaataaaGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt                 <  1:1684216/140‑1 (MQ=255)

                                                                                                                       taaaataaaGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt                 <  2:1330029/140‑1 (MQ=255)

                                                                                                                                 tagtctcATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTaataat       <  2:1982130/137‑1 (MQ=255)

                                                                                                                                    tctcATGCTTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                          >  1:1873217/1‑38 (MQ=255)

                                                                                                                                     ctcATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGa   >  2:2081328/1‑140 (MQ=255)

                                                                                                                                      tcATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                              >  1:1701842/1‑80 (MQ=255)

                                                                                                                                      tcATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGAt  >  1:3425816/1‑140 (MQ=255)

CAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGAT  >  NZ_CP025268/2862594‑2862866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`