BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	400,598	+G	pseudogene (162/260 nt)	iraP →	anti‑adapter protein IraP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	400,594	1	.	G	96.1%	409.4 / 8.7	127	pseudogene (158/260 nt)	iraP	anti‑adapter protein IraP
Reads supporting (aligned to +/- strand): ref base . (4/1); new base G (70/52); total (74/53)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

GTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NZ_CP025268/400457‑400728

gttgttATTTAAGCTTGCCCAACAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCTCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg                                                                                                                                         <  2:2866952/140‑1 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑ggtggcg                                                                                                                                    <  2:4808182/140‑6 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg‑g‑ggcg                                                                                                                                  <  1:4384086/140‑4 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg‑g‑ggcg                                                                                                                                  <  2:3141322/140‑4 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg‑g‑ggcg                                                                                                                                  <  2:3140932/140‑4 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg‑g‑ggcg                                                                                                                                  <  1:901758/140‑4 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg‑g‑ggcg                                                                                                                                  <  1:901887/140‑4 (MQ=255)

      aTTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg‑g‑ggcgc                                                                                                                                 >  2:2513177/1‑136 (MQ=255)

         tAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGt                                                                                                                              >  2:4049849/1‑140 (MQ=255)

           aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCTCAGGTAGAAGCTTTGTAGATAATCGTCACTGCAATGCTTCGCAATATGGCGCAACATGACCAACAGCGGTTGATTGATCAGGTAGA‑GG‑GCG‑CGCTGTAc                                                                                                                            >  2:4359207/1‑140 (MQ=255)

           aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTAc                                                                                                                            >  1:444649/1‑140 (MQ=255)

           aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTAc                                                                                                                            <  1:1215739/140‑1 (MQ=255)

              ttGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAg                                                                                                                         >  1:695828/1‑140 (MQ=255)

                gCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTGCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGt                                                                                                                       >  1:3071659/1‑140 (MQ=255)

                 cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTa                                                                                                                      >  1:1053616/1‑140 (MQ=255)

                 cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTa                                                                                                                      >  1:3008843/1‑140 (MQ=255)

                 cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTa                                                                                                                      >  2:2733922/1‑140 (MQ=255)

                      aaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGcc                                                                                                                 >  2:4559445/1‑140 (MQ=255)

                      aaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGcc                                                                                                                 <  2:3584982/140‑1 (MQ=255)

                       aagaagaagAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGccc                                                                                                                <  2:1514936/140‑1 (MQ=255)

                          aagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGAt                                                                                                             <  1:4934558/140‑1 (MQ=255)

                               gagTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAg                                                                                                        <  2:4694310/140‑1 (MQ=255)

                                 gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      <  1:1692333/140‑1 (MQ=255)

                                  tCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCATTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GG‑G‑GACGCTGTTCGAGGGAAAGCACGATGCCAGCAt                                                                                                     >  2:1722786/1‑140 (MQ=255)

                                  tCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCAt                                                                                                     >  1:89183/1‑140 (MQ=255)

                                      aaGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCt                                                                                                 <  1:1924492/140‑1 (MQ=255)

                                        gAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga                                                                                               <  2:5049644/140‑1 (MQ=255)

                                        gAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga                                                                                               <  1:4169530/140‑1 (MQ=255)

                                         aaCTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg‑g‑ggcg                                                                                                                                  >  1:2269773/1‑101 (MQ=255)

                                           cTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  1:3390714/140‑1 (MQ=255)

                                            tgtgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacgac                                                                                           >  2:3463204/1‑140 (MQ=255)

                                            tgtgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacgac                                                                                           >  2:3597245/1‑140 (MQ=255)

                                              tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGAcgacga                                                                                         >  1:5032253/1‑140 (MQ=255)

                                              tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGAcgacga                                                                                         >  1:5032875/1‑140 (MQ=255)

                                                tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       >  2:3903241/1‑140 (MQ=255)

                                                tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       <  2:1367169/140‑1 (MQ=255)

                                                tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGCAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       >  2:4300207/1‑140 (MQ=255)

                                                   gcAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTCGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACgg                                                                                    >  1:1172837/1‑140 (MQ=255)

                                                     aGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAg                                                                                  >  2:3477861/1‑140 (MQ=255)

                                                        tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAg                                                                                                        >  1:470067/1‑115 (MQ=255)

                                                        tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAg                                                                                                        <  2:470067/115‑1 (MQ=255)

                                                        tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGGAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  1:3250688/1‑140 (MQ=255)

                                                          gAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            >  1:4265321/1‑125 (MQ=255)

                                                          gAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  2:4265321/125‑1 (MQ=255)

                                                            aGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CACTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCtgctgc                                                                           >  1:3212206/1‑140 (MQ=255)

                                                            aGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CACTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCtgctgc                                                                           >  1:3212037/1‑140 (MQ=255)

                                                               tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcg                                                                        >  1:3607587/1‑140 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            >  1:2470448/1‑118 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:335287/1‑140 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:4425912/1‑140 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAACATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGCAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcgcc                                                                      >  2:1636245/1‑138 (MQ=255)

                                                                  ggAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  1:3597245/140‑1 (MQ=255)

                                                                  ggAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  1:4688956/140‑1 (MQ=255)

                                                                        tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaa                                                               >  1:1340886/1‑140 (MQ=255)

                                                                          tCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                             <  2:3023084/140‑1 (MQ=255)

                                                                                   cAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTg                                                    >  1:1649370/1‑140 (MQ=255)

                                                                                     aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  <  2:1053616/140‑1 (MQ=255)

                                                                                     aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  <  1:2588490/140‑1 (MQ=255)

                                                                                     aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  <  1:2670452/140‑1 (MQ=255)

                                                                                        cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCa                                               >  2:1147060/1‑140 (MQ=255)

                                                                                        cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCa                                               >  2:2193140/1‑140 (MQ=255)

                                                                                          tCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATc                                             <  1:4422032/140‑1 (MQ=255)

                                                                                           cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  2:2571570/1‑140 (MQ=255)

                                                                                           cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  2:728466/1‑140 (MQ=255)

                                                                                           cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  1:285416/1‑140 (MQ=255)

                                                                                           cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCAGcc                                            >  1:615183/1‑140 (MQ=255)

                                                                                           cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTACAGGG‑G‑G‑CGCTGTGCGAGGTAAAGCGCGATGCCAGCATTCGTGACGACGATACGGAGCTGCTGCGCGATTACGCAAAGAAGTTATTGAAGCAtac                                            >  2:3695264/1‑138 (MQ=255)

                                                                                           cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGGAGAGGG‑G‑G‑CGCTGTACGAGGGAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGCAAAGAAGTTATTGAAGCATcc                                            >  2:727411/1‑140 (MQ=255)

                                                                                             cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg‑g‑gg                                                                                                                                    >  2:211646/1‑49 (MQ=255)

                                                                                               atatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGt                                        <  1:4668010/140‑1 (MQ=255)

                                                                                                  tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  2:2376700/1‑140 (MQ=255)

                                                                                                  tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  1:525678/1‑140 (MQ=255)

                                                                                                   ggCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGt                                    >  1:5154567/1‑140 (MQ=255)

                                                                                                    gcgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTa                                   <  1:2534500/140‑1 (MQ=255)

                                                                                                     cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgc                                                                                 >  2:652642/1‑93 (MQ=255)

                                                                                                     cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgc                                                                                 <  1:3286649/93‑1 (MQ=255)

                                                                                                     cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgc                                                                                 >  2:3286649/1‑93 (MQ=255)

                                                                                                     cgcAAAATGACCAACAGCGGTTGATTGAGCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGAGGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  2:1377579/1‑140 (MQ=255)

                                                                                                          aaTGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGt                             >  2:2442943/1‑140 (MQ=255)

                                                                                                            tGACCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTa                           >  1:4956341/1‑140 (MQ=255)

                                                                                                              aCCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGCCGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAAt                         <  2:3071659/140‑1 (MQ=255)

                                                                                                              aCCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAAt                         <  1:327055/140‑1 (MQ=255)

                                                                                                              aCCAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAAt                         <  2:4418502/140‑1 (MQ=255)

                                                                                                               ccAACAGCGGTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCAGCCTCGTCAGTAAAAAGTTAATc                        <  2:1904781/140‑1 (MQ=255)

                                                                                                               ccAACAGCGGTTGATTGATCAGGTAGA‑GG‑G‑GTCGCTGTACGAGGTAAAGCCCGATGCCAACATTCCTGACGACGATACGGAGCTGCTGCGCTATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGGAAAAAGTTAATc                        >  1:2039564/1‑140 (MQ=255)

                                                                                                               ccAACAGCGGTTGATTGATCAGGCAGAGGG‑G‑G‑CGCTGTAAGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGACGCATCCTCGTCAGTAAAAAGTTAATc                        <  2:623566/140‑1 (MQ=255)

                                                                                                                       ggTTGATTGATAAGGTAGA‑GGCG‑G‑CGCTGTACGCGGTAACGCCCGATGCCAGCCTTACTGACGCCGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAAc                <  1:4077427/140‑1 (MQ=255)

                                                                                                                        gTTGATTGATCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGa                                                                                                              <  1:2740711/45‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGTCCACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:4304569/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:347563/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              >  1:138224/1‑140 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:1738767/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:2318931/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAACCCGATGCCAGAATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTAGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:2657816/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGG‑G‑G‑CACTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:3212037/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGG‑G‑G‑CACTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:3212206/140‑1 (MQ=255)

                                                                                                                          tgattgatCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGc             <  1:721120/140‑1 (MQ=255)

                                                                                                                            attgatCAGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTg           >  1:1123675/1‑140 (MQ=255)

                                                                                                                                   aGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaaa    <  2:138224/140‑1 (MQ=255)

                                                                                                                                   aGGTAGAGGG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaaa    <  2:2607128/140‑1 (MQ=255)

GTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GG‑G‑G‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NZ_CP025268/400457‑400728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`