BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,201	T→C	pseudogene (667/667 nt)	CXP41_RS03085 ←	class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,201	0	T	C	99.7%	1151.1 / NA	339	pseudogene (667/667 nt)	CXP41_RS03085	class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (152/186); minor base A (0/1); total (152/187)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

CGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑T‑G‑ATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGA  >  NZ_CP025268/581073‑581333

cGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTc                                                                                                                            >  1:784066/1‑140 (MQ=255)

   gtgtTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTg                                                                                                                         <  1:4028629/140‑1 (MQ=255)

        gCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCg                                                                                                                    <  2:2676416/140‑1 (MQ=255)

         cTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCgg                                                                                                                   >  2:3394273/1‑140 (MQ=255)

           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                 <  2:1486301/140‑1 (MQ=255)

           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                 <  1:4071333/140‑1 (MQ=255)

           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                 <  1:3384198/140‑1 (MQ=255)

           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                 <  2:491083/140‑1 (MQ=255)

             aCCGTGTTGATACATCCACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                               <  1:2865142/140‑1 (MQ=255)

             aCCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                               <  2:580727/140‑1 (MQ=255)

                gtgtTGATACATCAACTGCGCCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                            <  2:3609637/140‑1 (MQ=255)

                gtgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                            <  2:1637921/140‑1 (MQ=255)

                gtgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                            <  1:4711594/140‑1 (MQ=255)

                gtgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                            <  1:2425528/140‑1 (MQ=255)

                gtgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                            <  2:2745666/140‑1 (MQ=255)

                gtgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                            <  1:1829643/140‑1 (MQ=255)

                 tgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGCAGCGGAAAACAtt                                                                                                           >  1:2580438/1‑140 (MQ=255)

                 tgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGACAACAtt                                                                                                           >  1:4875116/1‑140 (MQ=255)

                 tgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                           >  2:3869625/1‑140 (MQ=255)

                 tgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                           >  2:2825247/1‑140 (MQ=255)

                 tgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                           >  1:1048548/1‑140 (MQ=255)

                 tgtTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                           >  1:3674775/1‑140 (MQ=255)

                     gATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                       <  1:174234/140‑1 (MQ=255)

                      aTACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                      <  1:4674506/140‑1 (MQ=255)

                       tACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                     <  1:421319/140‑1 (MQ=255)

                       tACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                     <  1:3552120/140‑1 (MQ=255)

                       tACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                     <  2:3675704/140‑1 (MQ=255)

                       tACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                     <  1:2703069/140‑1 (MQ=255)

                       tACATCAAATGAACAGGATATTAAGTAGACCTAC‑AAACCTGTCAGGGAGTAAGCAGCGGGATTATGCTGTAGCAGGCCTTAAGCAAGAACATGCTGC‑GC‑TCG‑G‑ATGCTATTTCTTTCCTGAAGCGGTAATCTTtcctacg                                                                                                     <  2:3973313/140‑2 (MQ=255)

                       caCATCAACTGCACTTGATATTGAGT‑CGCCTACGAACCCTGCCAGGGAGTAATCCTTGGCATTATGCCGCAGCACCTCTTAAGCAAGAACATGCTGC‑GGCT‑G‑G‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                     <  1:1881318/139‑1 (MQ=255)

                        aCATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                    <  1:5170732/140‑1 (MQ=255)

                           tCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                 >  1:544442/1‑140 (MQ=255)

                                  cACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                          >  1:3209182/1‑140 (MQ=255)

                                  cACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                          >  2:2343410/1‑140 (MQ=255)

                                   aCCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                         <  1:4542771/140‑1 (MQ=255)

                                   aCCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                         <  1:2784831/140‑1 (MQ=255)

                                    ccTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                        >  2:1318262/1‑140 (MQ=255)

                                    ccTGATATTGAGT‑GGCCTACGAAACCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                        <  2:805670/140‑1 (MQ=255)

                                     cTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCtt                                                                                       <  1:2813474/140‑1 (MQ=255)

                                            tGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                                >  1:1134099/1‑140 (MQ=255)

                                            tGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                                <  2:1189116/140‑1 (MQ=255)

                                            tGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                                >  2:1017272/1‑140 (MQ=255)

                                              aGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                              >  1:3950566/1‑140 (MQ=255)

                                              aGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                              >  1:4799654/1‑140 (MQ=255)

                                              aGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                              >  1:902504/1‑140 (MQ=255)

                                               gT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACa                                                                             >  2:2463179/1‑140 (MQ=255)

                                                   gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTTAAGCGGAAAACATTACTACAGTACCTTGAACCTTTGTTTTAACATtc                                                                          >  1:1174035/1‑140 (MQ=255)

                                                   gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                          >  2:1339840/1‑140 (MQ=255)

                                                   gCCTACGAACCCTGTCAGGGAGCAATCATTGGGATTATGCCGCAGCCCGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                          >  2:5028037/1‑140 (MQ=255)

                                                    ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                         >  1:1610238/1‑140 (MQ=255)

                                                       aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                      <  2:156370/140‑1 (MQ=255)

                                                         gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                    <  1:1414766/140‑1 (MQ=255)

                                                         gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                    <  2:5102101/140‑1 (MQ=255)

                                                             ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                <  1:3441947/140‑1 (MQ=255)

                                                             ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                <  1:3331018/140‑1 (MQ=255)

                                                             ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                <  1:3238846/140‑1 (MQ=255)

                                                              cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                               <  2:3013097/92‑1 (MQ=255)

                                                              cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                               >  1:3013097/1‑92 (MQ=255)

                                                              cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                               >  1:858696/1‑140 (MQ=255)

                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                              <  2:2238017/140‑1 (MQ=255)

                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                              <  2:3565122/140‑1 (MQ=255)

                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                              <  2:884173/140‑1 (MQ=255)

                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                              <  1:3656196/140‑1 (MQ=255)

                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                              <  1:124857/140‑1 (MQ=255)

                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                              <  1:3802455/140‑1 (MQ=255)

                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                              >  1:4376242/1‑140 (MQ=255)

                                                               tGTAAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGAGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACCTTCTCGAAATGctct                                                              <  2:187502/140‑1 (MQ=255)

                                                                gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                             >  2:2900726/1‑140 (MQ=255)

                                                                gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTATTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                             >  2:4800164/1‑140 (MQ=255)

                                                                 tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTGAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                            <  2:687679/140‑1 (MQ=255)

                                                                 tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                            <  1:1965919/140‑1 (MQ=255)

                                                                 tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                            <  1:693344/140‑1 (MQ=255)

                                                                  cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑TAG‑G‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                           <  1:4306846/140‑1 (MQ=255)

                                                                  cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTTGTTTTAACATTCTCGAAATGCTCTgag                                                           >  2:3367588/1‑140 (MQ=255)

                                                                  cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                           <  2:2040950/140‑1 (MQ=255)

                                                                  cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                           <  2:3209310/140‑1 (MQ=255)

                                                                     ggAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGt                                                        >  1:363678/1‑140 (MQ=255)

                                                                       aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                      <  1:2188746/140‑1 (MQ=255)

                                                                        gTAATCATTGGGATTATGCCGCAGCCCGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                     <  1:658281/140‑1 (MQ=255)

                                                                        gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                     <  1:714715/140‑1 (MQ=255)

                                                                         tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                    <  2:2626912/92‑1 (MQ=255)

                                                                           aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                  >  1:1272834/1‑140 (MQ=255)

                                                                           aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                  >  2:2409279/1‑140 (MQ=255)

                                                                           aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                  >  2:1394600/1‑140 (MQ=255)

                                                                                tGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTaa                                             >  1:1333916/1‑140 (MQ=255)

                                                                                 gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAg                                            >  2:4197497/1‑140 (MQ=255)

                                                                                   gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGcc                                          <  1:963809/140‑1 (MQ=255)

                                                                                             cagcaCGTCTTAAGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                                >  2:446395/1‑140 (MQ=255)

                                                                                                    tCTGAAGCATGAACAGCCTGC‑GG‑T‑T‑GTATGCTAGTTTTGTCCTGAAGGGGAAACCATTACTACAGTATCCTGAACCTTGGTTTTAATATTCTCGAACTGCCCGGAGAGTATATGTGTTAAGCCCTCTTCGTTATCTTTtg                         <  2:4353931/140‑1 (MQ=255)

                                                                                                         aGCAAGAACATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGttt                    <  2:3491089/140‑1 (MQ=255)

                                                                                                               aaCATGCTGCGGG‑T‑G‑G‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg              >  2:2102213/1‑140 (MQ=255)

                                                                                                               aaCATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg              >  2:4107808/1‑140 (MQ=255)

                                                                                                               aaCATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCCCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTCTGAAAAg              >  1:2868652/1‑140 (MQ=255)

                                                                                                                aCATGCTGC‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGa             <  1:1447997/140‑1 (MQ=255)

                                                                                                                     cggc‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCGTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTGGGAATCTTTTGTGTTTGAAAAGATGCCt        >  2:3099647/3‑140 (MQ=255)

                                                                                                                      tgc‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCtt       >  2:1239431/1‑140 (MQ=255)

                                                                                                                       gc‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGATTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt      <  1:215681/140‑1 (MQ=255)

                                                                                                                       gc‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt      <  1:1519455/140‑1 (MQ=255)

                                                                                                                       gc‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCCAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt      <  1:4121800/140‑1 (MQ=255)

                                                                                                                        c‑GG‑T‑TGG‑ATGCTATTTTTTTCCTGAAGCGGCAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTc     >  1:3090117/1‑140 (MQ=255)

CGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGT‑GGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GG‑T‑T‑G‑ATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGA  >  NZ_CP025268/581073‑581333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`