BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	607,037	A→G	51 (TAA→TAG)	hokE →	type I toxin‑antitoxin system toxin HokE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	607,037	0	A	G	100.0%	1405.9 / NA	419	51 (TAA→TAG)	hokE	type I toxin‑antitoxin system toxin HokE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (216/203); total (216/203)

Only 100 of 419 total aligned reads displayed.

CCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAA  >  NZ_CP025268/606901‑607173

    tGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTtgt                                                                                                                                   >  1:1203619/1‑140 (MQ=255)

     gCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGGtgtg                                                                                                                                  >  2:1773625/1‑140 (MQ=255)

      cGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGc                                                                                                                                 <  1:841439/140‑1 (MQ=255)

       ggCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCg                                                                                                                                <  2:2210808/140‑1 (MQ=255)

             cATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGt                                                                                                                          <  2:2370247/140‑1 (MQ=255)

             cATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGt                                                                                                                          <  2:537436/140‑1 (MQ=255)

             cATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGt                                                                                                                          <  2:669767/140‑1 (MQ=255)

               tAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTaa                                                                                                                        <  1:1022779/140‑1 (MQ=255)

                      gtgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATa                                                                                                                 >  2:2640580/1‑140 (MQ=255)

                       tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATaa                                                                                                                >  2:33757/1‑140 (MQ=255)

                       tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATaa                                                                                                                >  1:1600347/1‑140 (MQ=255)

                       tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATaa                                                                                                                >  1:4399265/1‑140 (MQ=255)

                       tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCAGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATaa                                                                                                                >  2:33462/1‑140 (MQ=255)

                        gtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAg                                                                                                               >  2:1601775/1‑140 (MQ=255)

                         tttAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAgc                                                                                                              <  1:3219243/140‑1 (MQ=255)

                          ttAACGGTGCTGGGATTTACGCTTCTGGTCGGATACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGATCCGAAGAAGTAGCCGTTGTGCGGGGAGTACTCCCATAAgcg                                                                                                             <  2:3159463/140‑1 (MQ=255)

                          ttAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAgcg                                                                                                             <  2:3276586/140‑1 (MQ=255)

                           tAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAgcgc                                                                                                            <  2:1321872/140‑1 (MQ=255)

                           tAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAgcgc                                                                                                            <  1:721648/140‑1 (MQ=255)

                           tAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAgcgc                                                                                                            <  1:3576428/140‑1 (MQ=255)

                            aaCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGa                                                                                                                            <  2:5039322/123‑1 (MQ=255)

                            aaCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                           <  2:2890937/140‑1 (MQ=255)

                            aaCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                                           <  2:303960/140‑1 (MQ=255)

                            aaCGGTGCTGGCAGTCACGCTTCTGGTCGGAGCCTCGCTGTGTGAGTTTACGGTCAAGGAACGTAATATTGAGTTTACGGCTGTCCTCGCTTACCAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAGGCGCt                                                                                                           <  2:1439385/140‑1 (MQ=255)

                              cGGTGCTGGGATTGACGCGTCTGGTCCGCGACTCGCGGTGGGCTTTTACGGGCAAGGAACGGCGTATCGAGCTTAAGGCTGTTCTCGCTTACGAACCGCAGAAGGAGCCGTTGTGCGGGGAGTAACACCATAAGCGCTaa                                                                                                         >  2:4759676/1‑140 (MQ=255)

                                 tGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACtt                                                                                                      >  1:4715118/1‑140 (MQ=255)

                                 tGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAATTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACtt                                                                                                      >  2:4624675/1‑140 (MQ=255)

                                 cgCTGGGATTTACGCTTATGGCCTGAGACTCGCGGTGTGAGTTTACGGTGAAGGAACGTAATCGTGAGTTTAAGGCTGTTCTCGCTTACGAACCGCAGACGTAGCCGATGTGCGTGGAGTACTCCCATAAGCGTTCACtt                                                                                                      >  2:4192400/2‑140 (MQ=255)

                                  gCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                                     <  1:514621/140‑1 (MQ=255)

                                   cTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGCACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTaa                                                                                                    >  2:4986175/1‑140 (MQ=255)

                                   cTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTaa                                                                                                    <  1:2316522/140‑1 (MQ=255)

                                   cTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAACTAACGGAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGTAGAAGTAGCCGTTGTGCGGGGAGTAATCCCAGAAGCGCTAACGTaa                                                                                                    >  2:3962124/1‑140 (MQ=255)

                                      ggATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                                                 <  2:1878747/140‑1 (MQ=255)

                                         tttACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtg                                                                                              <  2:3486744/140‑1 (MQ=255)

                                         tttACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtg                                                                                              <  1:1157816/140‑1 (MQ=255)

                                          ttACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgt                                                                                             <  2:3534909/140‑1 (MQ=255)

                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGGAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTCAGGGTtgtg                                                                                            >  1:4966883/1‑140 (MQ=255)

                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgtg                                                                                            >  1:3060065/1‑140 (MQ=255)

                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgtg                                                                                            >  1:4836798/1‑140 (MQ=255)

                                               cTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                                        <  2:3725831/140‑1 (MQ=255)

                                                  cTGGTCGGAGTCTCGCTGTGTGTGTTTACGGTGAAGGAACGTAAGATTGAGGTGAAGGCTGTTCTCGCTTACGAAGCGAAGAAGTAGGCGGCGTGCGGGTAGGAATCCGATAAGTTCTAACTGAAGGGTTGTGGTATTAc                                                                                     >  2:51716/1‑140 (MQ=255)

                                                  cTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTAc                                                                                     <  1:970419/140‑1 (MQ=255)

                                                  cTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATGGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAGGGGTTGTGGTATTAc                                                                                     >  2:4085858/1‑140 (MQ=255)

                                                   tGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACg                                                                                    <  1:2637123/140‑1 (MQ=255)

                                                     gTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAGCGTAATATTGAGTTTAAGGGTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGGGGGGAGTAATCCCATAAGCGCTAACTTAAAGGTTGTGGGATTACGcc                                                                                  >  2:1190089/1‑140 (MQ=255)

                                                     gTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGcc                                                                                  <  1:3329948/140‑1 (MQ=255)

                                                         gagaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGACGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGat                                                                              <  1:4731920/140‑1 (MQ=255)

                                                         gagaCTCGATATGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTTTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGat                                                                              <  2:3810463/140‑1 (MQ=255)

                                                          agaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                                                                             >  2:1158739/1‑140 (MQ=255)

                                                                gCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAttt                                                                       >  1:250742/1‑140 (MQ=255)

                                                                   gtgtgAGTGTACGGTGAAGGAACGTAATATTGAGTTTAAGGTTGTTCGCGATTACGAACCGATGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAc                                                                    <  2:4593686/140‑1 (MQ=37)

                                                                    tgtgAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACg                                                                   >  1:155274/1‑140 (MQ=255)

                                                                      tgAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTg                                                                 >  1:2960213/1‑140 (MQ=255)

                                                                              cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGaa                                                         >  1:2432626/1‑140 (MQ=255)

                                                                               ggTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt                                                        <  1:4167030/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATCTGATTTAACGTGCCGATGAATTAct                                                    <  2:4675963/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    >  2:76303/1‑140 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  1:1772998/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  1:5206678/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  1:5026745/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  1:463094/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  1:311360/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  1:3088369/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  1:2806282/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  1:2768635/140‑1 (MQ=255)

                                                                                   aaGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                    <  2:702088/140‑1 (MQ=255)

                                                                                         cGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACg                                              >  1:3933370/1‑140 (MQ=255)

                                                                                           tAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAt                                            >  2:3663389/1‑140 (MQ=255)

                                                                                                ttGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTg                                       <  2:864281/140‑1 (MQ=255)

                                                                                                 tGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTgg                                      >  2:1469172/1‑140 (MQ=255)

                                                                                                 tGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTgg                                      >  1:4302589/1‑140 (MQ=255)

                                                                                                    gTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCa                                   >  1:1808454/1‑140 (MQ=255)

                                                                                                     tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                  >  1:2144773/1‑140 (MQ=255)

                                                                                                     tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                  <  1:3406998/140‑1 (MQ=255)

                                                                                                     tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                  >  1:3642077/1‑140 (MQ=255)

                                                                                                     tttAAGGCTGTGCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                  <  2:2483473/140‑1 (MQ=255)

                                                                                                       tAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCa                                >  1:4506598/1‑140 (MQ=255)

                                                                                                          ggCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                             >  1:1100998/1‑140 (MQ=255)

                                                                                                          ggCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                             <  1:1873214/140‑1 (MQ=255)

                                                                                                          ggCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                             <  1:3689409/140‑1 (MQ=255)

                                                                                                           gCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTc                            <  1:1978920/140‑1 (MQ=255)

                                                                                                             tGTTCTCGCTTACGAACCGAAGACGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTg                          >  2:2036928/1‑140 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAGGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        >  1:3798628/1‑140 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTCCGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        <  2:826759/140‑1 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        <  2:3892730/140‑1 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        <  2:4387434/140‑1 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        <  2:45109/140‑1 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        >  1:4547892/1‑140 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        >  1:616108/1‑140 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        <  2:3623901/140‑1 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        >  1:3322361/1‑140 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        <  1:2702566/140‑1 (MQ=255)

                                                                                                               ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                        <  1:788538/140‑1 (MQ=255)

                                                                                                                   cGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCat                    <  1:2574077/140‑1 (MQ=255)

                                                                                                                         cGAACCGGAGAAGTAGCCGTTGTGCGGGGAGTAACCCCATGCGCGCTAACTTAAGGGTTATGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACGGTCACGATAACTGGCCAGCAATTCTGGCCCATATTgat              <  2:1834600/140‑3 (MQ=17)

                                                                                                                         cGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCCTAAGCGCTAACTTAAGGGTGGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGt              >  2:1567537/1‑140 (MQ=255)

                                                                                                                         cGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGt              >  1:1346360/1‑140 (MQ=255)

                                                                                                                         cGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGt              >  2:1535341/1‑140 (MQ=255)

                                                                                                                         cGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGt              <  1:403755/140‑1 (MQ=255)

                                                                                                                                aagaagTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCg       >  2:1746703/1‑140 (MQ=255)

CCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAA  >  NZ_CP025268/606901‑607173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`