BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	832,209	A→G	V336A (GTG→GCG)	ybhR ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	832,209	0	A	G	99.3%	909.5 / NA	283	V336A (GTG→GCG)	ybhR	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (155/125); total (156/127)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

GCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAA  >  NZ_CP025268/832076‑832345

gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCt                                                                                                                                    >  2:2040366/1‑140 (MQ=255)

gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCt                                                                                                                                    <  1:1038644/140‑1 (MQ=255)

gcggTAGTGTCTTTCCTGGCCCAAGCTAAGACGTTACATCACCTTACGTCTCAACATCGCGTACGCTGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCt                                                                                                                                    <  2:1161573/140‑1 (MQ=255)

      gtgtCTTTGCTGGCGAAAGATAAGAAGTTACATCAGCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACCCAATATCCAGACTCGCATCCTTCaaat                                                                                                                              >  2:1635905/1‑140 (MQ=255)

      gtgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaat                                                                                                                              >  1:3431256/1‑140 (MQ=255)

        atCTTTACTGGCGACATATAAGAAATTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataa                                                                                                                            <  2:2053718/139‑1 (MQ=255)

          ctcaccTGGCGAAAGATAAGAAGACACATCAACGTACGTCAAAACATCGCGCACGCCGCTCACACTGTCGTGGCCCTTATCACCATTAACATCCACAAACTATTCCAATCAATATCCATACTCGCATCCTTCaaataaat                                                                                                                          <  2:3073049/135‑1 (MQ=255)

          cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  1:3304890/140‑1 (MQ=255)

          cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          >  2:3378119/1‑140 (MQ=255)

              gCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                                                                      <  1:4809322/140‑1 (MQ=255)

              gCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                                                                      >  1:216514/1‑140 (MQ=255)

               cTGGCGAAAGATAAGAAGTTACTTCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                                                                     <  2:4259462/140‑1 (MQ=255)

               cTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGGTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATACTTCAAATAAATCTGCt                                                                                                                     <  1:147231/140‑1 (MQ=255)

                 ggCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTg                                                                                                                   >  1:609644/1‑140 (MQ=255)

                 ggCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTg                                                                                                                   >  2:4224251/1‑140 (MQ=255)

                   cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCGCACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGt                                                                                                                 >  2:3308909/1‑140 (MQ=255)

                   cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  1:49704/131‑1 (MQ=255)

                   cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          >  2:49704/1‑131 (MQ=255)

                   cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGt                                                                                                                 >  1:254048/1‑140 (MQ=255)

                   cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGt                                                                                                                 <  1:5170936/140‑1 (MQ=255)

                   cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGt                                                                                                                 >  1:3955053/1‑140 (MQ=255)

                     aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATTAATCTGCTTGGTaa                                                                                                               <  2:569950/140‑1 (MQ=255)

                     aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                                                                               <  2:1008361/140‑1 (MQ=255)

                       aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGGTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATg                                                                                                             >  1:4439989/1‑140 (MQ=255)

                        gATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACACTATCCAGCCTCGCATCCTTCAAATAAATCTGCTTGGTAATGt                                                                                                            <  2:4549344/140‑1 (MQ=255)

                            agaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTTGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCAGCCTTCAAATAAATCTGCGTGGTAATGTCCGt                                                                                                        >  2:474979/1‑140 (MQ=255)

                               agTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                                                                     <  2:2231838/140‑1 (MQ=255)

                               agTTACATCACCGTACGTCTAAATAGCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAAGCTGCTTGGTAATGTCCGTaaa                                                                                                     <  1:2693908/140‑1 (MQ=255)

                                  tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                                  <  2:3615914/140‑1 (MQ=255)

                                  tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCATTCAAATAAATCTGCTGGGTAATGTCCGTAAAGTg                                                                                                  >  2:3484923/1‑140 (MQ=255)

                                  tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGGGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                                  <  1:2921584/140‑1 (MQ=255)

                                   aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                                                                 >  2:5060339/1‑140 (MQ=255)

                                   aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                                                                 >  2:2076802/1‑140 (MQ=255)

                                   aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                                                                 >  2:375477/1‑140 (MQ=255)

                                       cACCTTACGTCTCACCATCGCGTACGACGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  1:890519/140‑1 (MQ=255)

                                       cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTCGCGGCCACAAACTATTCCACACAATCTCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  1:805496/140‑1 (MQ=255)

                                       cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  1:1157044/140‑1 (MQ=255)

                                       cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  1:1047458/140‑1 (MQ=255)

                                       cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGGAATGTCCGTAAAGTGGCGaa                                                                                             <  2:4538126/140‑1 (MQ=255)

                                        aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            <  1:4938543/140‑1 (MQ=255)

                                        aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            <  2:747787/140‑1 (MQ=255)

                                        aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            >  2:640286/1‑140 (MQ=255)

                                        aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            <  1:1408841/140‑1 (MQ=255)

                                        aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            >  2:1836836/1‑140 (MQ=255)

                                           ttACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAgg                                                                                         >  2:4298323/1‑140 (MQ=255)

                                                    aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCa                                                                                >  2:2273744/1‑140 (MQ=255)

                                                    aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTACCGGCCACAAACTATTCCACACAATACGCAGACTCGCAGCCTGCAAATAAATCTGCTTGGTAATGCCCGTAAAGTGGCGAAGAGGGTTAAGCCa                                                                                >  2:226682/1‑140 (MQ=255)

                                                        ccgcgTACGCCGCTGGCCCTGTCGTGGCGGTTATCACCAGTAGCGGCCACAAACGAGTCCACGCAATATCCAGACTCGCATACTTCAAAGCGATCTCCTTGGTAACGTGAGTAAAGTGGCGACGAGGGTTAATCCGCGTc                                                                            >  2:4164478/2‑140 (MQ=255)

                                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                                                                           >  1:3494142/1‑140 (MQ=255)

                                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                                                                           >  1:2626916/1‑140 (MQ=255)

                                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                                                                           <  1:2040366/140‑1 (MQ=255)

                                                           cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAgg                                                                         >  1:3886322/1‑140 (MQ=255)

                                                              aCGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGttt                                                                      <  2:953171/140‑1 (MQ=255)

                                                               cgccgcGCACCCGCTCGTGGCCGTTCGCACCAGCACCGTCCCCAAACTATTCCACACCGTCTCCAGACTCACATCCTTCAAACAAGCCTGCATGGGATTGTCCGGAAAGTAGCGAGTCGGGTGACTCCTCGTGAgagttg                                                                     <  2:1540104/140‑6 (MQ=255)

                                                                gccgcTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTg                                                                    <  2:3210280/140‑1 (MQ=255)

                                                                    cTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGc                                                                >  2:3920506/1‑140 (MQ=255)

                                                                     tGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGcc                                                               >  1:2076437/1‑140 (MQ=255)

                                                                          cTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                                                                     >  1:3109223/1‑97 (MQ=255)

                                                                          cTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                                                                     <  2:3109223/97‑1 (MQ=255)

                                                                            gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCCCACAATATCCAGACTCGCATCCTCCAAATAAATCTGCTTGGTAATGTCCGCAAAGTGGCGAATAGGGATAATGCACGTCAGGTTTTGAGGCCATACCgg                                                        >  2:1237205/1‑140 (MQ=255)

                                                                            gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAGATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCgg                                                        >  2:4791625/1‑140 (MQ=255)

                                                                            gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCgg                                                        >  1:3017377/1‑140 (MQ=255)

                                                                            gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCgg                                                        >  1:3006606/1‑140 (MQ=255)

                                                                            gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCgg                                                        >  1:2976821/1‑140 (MQ=255)

                                                                            gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCgg                                                        >  2:4353363/1‑140 (MQ=255)

                                                                             ccGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTGCAAAGAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGc                                                       <  1:3047673/139‑1 (MQ=255)

                                                                                  gCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGtg                                                  >  1:4598899/1‑139 (MQ=255)

                                                                                   ccGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGttt                                                 <  1:5024970/140‑1 (MQ=255)

                                                                                      ttATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCAGACCGGCATGTTTTcc                                              >  2:1768434/1‑140 (MQ=255)

                                                                                       tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  1:4492974/1‑140 (MQ=255)

                                                                                       tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  1:55584/1‑140 (MQ=255)

                                                                                       tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGGAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  1:4905017/1‑140 (MQ=255)

                                                                                           aCCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCgg                                         >  1:2341226/1‑140 (MQ=255)

                                                                                            ccAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCTAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTTCACCGga                                        <  2:3512850/140‑1 (MQ=255)

                                                                                            ccAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGga                                        <  1:4516967/140‑1 (MQ=255)

                                                                                                   cGGCCACAAACTCTCCCACACAATATCCAGACTCGCATCCTTCAACTAAATCTGCTTGCTAATGTCCGTAAAGTCGCGAATCGGGTTAATCCACGTCAGGTTTTGCACCCATACCAGCATGTTTTCCACCGGAGAAACGt                                 <  2:1343873/140‑1 (MQ=255)

                                                                                                   cGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGt                                 >  1:638048/1‑140 (MQ=255)

                                                                                                     gcaacaAACTATTCCACAAAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               <  2:1932372/137‑1 (MQ=255)

                                                                                                     gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               >  1:2974068/1‑140 (MQ=255)

                                                                                                     gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               >  2:5000396/1‑140 (MQ=255)

                                                                                                     gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               >  1:1588371/1‑140 (MQ=255)

                                                                                                        acaAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCg                            >  2:3281083/1‑140 (MQ=255)

                                                                                                          aaaCTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGATTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                          <  2:2867936/140‑1 (MQ=255)

                                                                                                                ttCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGgag                    >  1:1754075/1‑140 (MQ=255)

                                                                                                                ttCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGgag                    >  2:2695889/1‑140 (MQ=255)

                                                                                                                tctcacacaCTATCCAGTTTCGCATCCTTCCCATATATCTCCTTGGTAATGTCCGTAAATTAGCGAATAGGGGTAATCCTGGCCATGTTTTTCAGCCATACCGGCATGTTTTCATCCGACGAAACGTAAGCGGAAAGtag                    <  2:2161141/137‑1 (MQ=255)

                                                                                                                 tCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGgaga                   <  2:1071976/140‑1 (MQ=255)

                                                                                                                     cacaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATgg               >  1:238014/1‑140 (MQ=255)

                                                                                                                        aaTATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCgg            >  1:188853/1‑140 (MQ=255)

                                                                                                                        aaTATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTGTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCgg            >  1:4328106/1‑140 (MQ=255)

                                                                                                                          tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTCATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  2:1107775/140‑1 (MQ=255)

                                                                                                                          tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  2:2357583/1‑140 (MQ=255)

                                                                                                                          tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  1:2747044/1‑140 (MQ=255)

                                                                                                                          tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  1:1947861/140‑1 (MQ=255)

                                                                                                                          tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  1:4033588/1‑140 (MQ=255)

                                                                                                                          tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  2:3431256/140‑1 (MQ=255)

                                                                                                                          tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTGGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  1:1599288/1‑140 (MQ=255)

                                                                                                                          tatCCAGACGCGCATCCTTCAAATAAATCTGCTTGGGAATGTGAGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTGTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  2:4920184/140‑1 (MQ=255)

                                                                                                                                gACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATa    >  1:3754417/1‑140 (MQ=255)

                                                                                                                                gACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGGAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCTACCGGAGAAACGTAACCGGAAAGGGGAATGGCGGCCGTCATa    >  2:4468176/1‑140 (MQ=255)

GCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAA  >  NZ_CP025268/832076‑832345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`