BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,109,323	C→T	E107E (GAG→GAA)	csgE ←	curli production assembly/transport protein CsgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,109,323	0	C	T	100.0%	630.9 / NA	172	E107E (GAG→GAA)	csgE	curli production assembly/transport protein CsgE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (76/96); total (76/96)

Only 100 of 175 total aligned reads displayed.

CGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTC  >  NZ_CP025268/1109188‑1109457

cGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGcttctt                                                                                                                                    <  1:3970039/140‑1 (MQ=255)

  aaaTAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCa                                                                                                                                  >  1:3077271/1‑140 (MQ=255)

  aaaTAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCa                                                                                                                                  >  1:1378622/1‑140 (MQ=255)

  aaaTAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCa                                                                                                                                  >  2:354601/1‑140 (MQ=255)

  aaaTAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCa                                                                                                                                  >  1:2346938/1‑140 (MQ=255)

     tAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGtt                                                                                                                               <  2:4719348/140‑1 (MQ=255)

          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                          <  2:1981311/140‑1 (MQ=255)

          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                          <  2:2727499/140‑1 (MQ=255)

          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                          <  1:2572130/140‑1 (MQ=255)

          aTGAGTAGAACGACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                          <  1:4682781/140‑1 (MQ=255)

             aGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAg                                                                                                                       >  2:1518260/1‑140 (MQ=255)

             aGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAg                                                                                                                       >  2:3882491/1‑140 (MQ=255)

                  aaCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCaa                                                                                                                  >  2:951108/1‑140 (MQ=255)

                   actactGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCaaa                                                                                                                 >  2:3834991/1‑140 (MQ=255)

                       ctGCATGTTTGACGCGCCTTGCAGCCTGCGAACACTTTTTTATTTCGAATTCATCATTCGCCACATCGCCCGTACTTAATAACTCCTGATTTACCTGGCGACGATTTAGTGCTACTTTAGTTTGAATCAGTGCAAAgacg                                                                                                             >  2:1010044/1‑140 (MQ=255)

                       ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacg                                                                                                             >  2:2505516/1‑140 (MQ=255)

                        tGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacga                                                                                                            >  1:3034051/1‑140 (MQ=255)

                        tGCATGCTTCACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGTGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacga                                                                                                            <  2:4959097/140‑1 (MQ=255)

                         gCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacgac                                                                                                           >  2:5149558/1‑140 (MQ=255)

                            tGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTATTAACTCCTGTTTTATCTGGATACGATTGAGCGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAga                                                                                                        >  2:184364/1‑139 (MQ=255)

                                    acGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGa                                                                                                >  1:3866834/1‑140 (MQ=255)

                                      gCATTGCAGCCTCCGAACAATTTTTTATGTAGAATTCATCAGGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAg                                                                                              >  1:921844/1‑140 (MQ=255)

                                       cATTTCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATGTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                             <  2:623366/140‑1 (MQ=255)

                                       cATTGCAGTCTCCGCACAATTTTTTATTTAGTATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCACTTTGAATCAGTGCAACGACGACAGTTTTCTCGAAGt                                                                                             <  2:2392354/140‑1 (MQ=255)

                                       cATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                             >  2:4763989/1‑140 (MQ=255)

                                       cATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                             >  2:420119/1‑140 (MQ=255)

                                       cATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                             >  2:220806/1‑140 (MQ=255)

                                       cATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                             <  2:2392483/140‑1 (MQ=255)

                                                  ccGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc                                                                                  <  1:4336867/140‑1 (MQ=255)

                                                  ccGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc                                                                                  <  1:116771/140‑1 (MQ=255)

                                                           tttttATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAAGGAAATaaa                                                                         <  1:2150837/140‑1 (MQ=255)

                                                                   taGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTg                                                                 <  2:771483/140‑1 (MQ=255)

                                                                     gAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGa                                                               <  1:1260037/140‑1 (MQ=255)

                                                                     gAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGa                                                               <  1:4047491/140‑1 (MQ=255)

                                                                      aaTTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGaa                                                              >  1:2657699/1‑140 (MQ=255)

                                                                      aaTTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATCTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGaa                                                              >  1:1317488/1‑140 (MQ=255)

                                                                         tcatcaTGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAAt                                                           <  1:951108/140‑1 (MQ=255)

                                                                         tcatcaTGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTGTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAAt                                                           <  1:3594772/140‑1 (MQ=255)

                                                                         tcatcaTGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAAACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAAt                                                           <  1:867010/140‑1 (MQ=255)

                                                                                gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATCACGtac                                                    >  2:4359561/1‑138 (MQ=255)

                                                                                gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTcc                                                    >  1:4550773/1‑140 (MQ=255)

                                                                                     agaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGGGCTTCTTCAGTTTGAATCAGTGAAAACACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCGGGATAATAACGTCCTGAtt                                               <  2:4318711/138‑1 (MQ=255)

                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGGTTATTTAGTTTTAAGAGGCGCAAAGACGACAGTTCTCTCGAAGTCTATTTTCAACGGAAATAAAAAAGGCTGGAAAATAGCGACCTGAtt                                               <  2:4727409/140‑1 (MQ=255)

                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                               <  2:2705164/140‑1 (MQ=255)

                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                               <  1:53534/140‑1 (MQ=255)

                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                               <  1:1033229/140‑1 (MQ=255)

                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                               <  1:2960967/140‑1 (MQ=255)

                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                               <  1:4312978/140‑1 (MQ=255)

                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGATTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                               <  1:4335133/140‑1 (MQ=255)

                                                                                     aaaTCGACCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTGTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                               <  1:1675300/140‑1 (MQ=255)

                                                                                      aaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATGAAAAAGTCGGGAAAATAACGTCCTGATTg                                              >  1:31038/1‑140 (MQ=255)

                                                                                        tCGCTCGTACTTAATACCGCCTTATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATACCGTCCTGATTGAc                                            <  2:2097856/140‑1 (MQ=255)

                                                                                        tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                            <  1:1558050/140‑1 (MQ=255)

                                                                                        tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                            <  2:3827614/140‑1 (MQ=255)

                                                                                        ccGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                            <  2:104853/139‑1 (MQ=255)

                                                                                          gCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCCACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCg                                          <  2:2463620/140‑1 (MQ=255)

                                                                                          gCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCg                                          <  1:1017714/140‑1 (MQ=255)

                                                                                            ccGTACTTAATAACGCCTGATTTATCTGGGGACGATTTAGTGCTTCTTAAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGtt                                        >  2:166367/1‑140 (MQ=255)

                                                                                             cGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTta                                       <  1:2786807/140‑1 (MQ=255)

                                                                                               tACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTtata                                     <  1:4781432/140‑1 (MQ=255)

                                                                                               tACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGCCCTGCTTGACCGTtata                                     <  2:11520/140‑1 (MQ=255)

                                                                                                acgtaataaCGCCTGAGGTATGTGGGGACGTTTTAGTGATTCTTCAGTTTGAATCAGTGCAAAGTCGGCAGTTGTCGCGGAGTCTGTTGGCGAAGGAAATGGTGGAGGCTGGAAAATAACGTCCTGATTGTCCGTTATAg                                    >  2:3205748/4‑140 (MQ=255)

                                                                                                      taaCGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATcc                              <  1:2087967/140‑1 (MQ=255)

                                                                                                        aCGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAg                            <  1:756170/140‑1 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACTATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                           >  2:3595719/1‑140 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTCGTGCTTCTTCAGTTTGAATCAGTGCAAACACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTTGAAAATAACGTCCTGATTGACCGTTATCGTGATTCAGc                           >  1:2730809/1‑140 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCa                                                                                                                   >  1:1941163/1‑52 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCACCGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                           >  1:637929/1‑140 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCg                                          <  2:4565373/125‑1 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCg                                          >  1:4565373/1‑125 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                           >  1:3184105/1‑140 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                           >  1:5104019/1‑140 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                           >  2:2703540/1‑140 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                           >  1:1473743/1‑140 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                           >  1:947881/1‑140 (MQ=255)

                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                           >  1:4175265/1‑140 (MQ=255)

                                                                                                           ccTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCtt                         <  1:4932143/140‑1 (MQ=255)

                                                                                                           ccTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCtt                         <  1:4260235/140‑1 (MQ=255)

                                                                                                            cTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTc                        >  2:1950262/1‑140 (MQ=255)

                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCACCGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       <  2:2231495/140‑1 (MQ=255)

                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       <  1:4228766/140‑1 (MQ=255)

                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       <  2:1697990/140‑1 (MQ=255)

                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       <  1:2379265/140‑1 (MQ=255)

                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       <  1:271184/140‑1 (MQ=255)

                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       <  1:3882602/140‑1 (MQ=255)

                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       <  2:4864792/140‑1 (MQ=255)

                                                                                                              gATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTccc                      >  1:4753965/1‑140 (MQ=255)

                                                                                                               aTTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       >  2:220331/1‑138 (MQ=255)

                                                                                                               aTTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                       <  1:220331/138‑1 (MQ=255)

                                                                                                                  tATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATc                  <  1:973526/140‑1 (MQ=255)

                                                                                                                  tATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCCGATTGACCGTTATAGTGATCCAGCTTCCCCATc                  <  1:4161252/140‑1 (MQ=255)

                                                                                                                   aTCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCg                 <  1:2270091/140‑1 (MQ=255)

                                                                                                                    tCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGt                >  2:4362188/1‑140 (MQ=255)

                                                                                                                       ggCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCa             <  1:3958108/140‑1 (MQ=255)

                                                                                                                         cgacgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGCCCGTTATAGTGATCCAGCTTCCCCATCGTGCACt           <  1:1603404/140‑1 (MQ=255)

                                                                                                                         cgacgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACt           <  1:595974/140‑1 (MQ=255)

                                                                                                                          gacgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTg          <  1:1859361/140‑1 (MQ=255)

                                                                                                                          gacgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTg          <  2:2414246/140‑1 (MQ=255)

                                                                                                                               tttAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCt     <  1:354601/140‑1 (MQ=255)

                                                                                                                                  aGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTc  >  1:5091692/1‑140 (MQ=255)

CGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTC  >  NZ_CP025268/1109188‑1109457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`