BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,252,698	A→G	147 (TAA→TAG)	CXP41_RS06625 →	protein YcgY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,252,698	0	A	G	99.5%	642.8 / NA	190	147 (TAA→TAG)	CXP41_RS06625	protein YcgY
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (101/88); minor base T (1/0); total (102/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

ATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGT  >  NZ_CP025268/1252562‑1252833

    ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAAc                                                                                                                                  >  1:4345807/1‑140 (MQ=255)

      aaCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCa                                                                                                                                <  1:1045974/140‑1 (MQ=255)

      aaCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCa                                                                                                                                <  1:1171598/140‑1 (MQ=255)

      aaCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCa                                                                                                                                <  1:3379212/140‑1 (MQ=255)

      aaCGACGGCGTAAATCGCCGGGTCATGAAATTGAAACACTTACCCGTAGAAATGACGCTATCTCCGTCAACCACGTATGGCAACCATTTGCCCAATACTTCCCGCCAGGACTTGAAGACCAGTATGTGTAGAAAAAACCa                                                                                                                                <  2:698727/140‑1 (MQ=255)

       aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTTCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               >  1:2725403/1‑140 (MQ=255)

       aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               >  1:1016962/1‑140 (MQ=255)

       aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               >  1:3685452/1‑140 (MQ=255)

       aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAt                                                                                                                               <  2:2256004/140‑1 (MQ=255)

         gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             <  2:4155464/140‑1 (MQ=255)

         gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             >  1:3979177/1‑140 (MQ=255)

         gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             <  2:4523931/140‑1 (MQ=255)

         gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             >  1:5211342/1‑140 (MQ=255)

         gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAAAATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAg                                                                                                                             >  2:331106/1‑140 (MQ=255)

           tggCGTAAATAGCCGTTTAATCAAAGTCAAAAACTTAACCGCAGAGCTGACGCTATCTTCCGCAACAACGAATAGCAAACTTTTGCGCAATACCTCCCGCCAGGACTTGAAAAACCGTCTGTGCAGAAAACACCAtccct                                                                                                                           <  2:1464451/139‑5 (MQ=255)

           cGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCt                                                                                                                           <  1:2361192/140‑1 (MQ=255)

           cGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCACAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCt                                                                                                                           <  1:974295/140‑1 (MQ=255)

           aggCGTACATCGCCGGGTGATGCGAGTGAAACACTTACCCGTAACAATGACACTATCTTCGGCCACCACGTATGGCAAACAGTTGCGCAATACTTCCCGCCAGGACTTGAAAATCCGTCTGTGTAGAAAAAACCATAGCt                                                                                                                           <  2:3331352/139‑1 (MQ=255)

            ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTa                                                                                                                          <  2:2341647/140‑1 (MQ=255)

              cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATg                                                                                                                        <  1:3038984/140‑1 (MQ=255)

              cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATg                                                                                                                        <  2:3967453/140‑1 (MQ=255)

              cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATg                                                                                                                        >  2:747564/1‑140 (MQ=255)

              cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACAACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATg                                                                                                                        >  2:715576/1‑140 (MQ=255)

              cGTAAACCGCCGGGTGCTGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCACACATTTGCGCAATACCTCCCGCCAGCACTTGAACAACCGGCTGTGTAGAAAAAACCACAgacatg                                                                                                                        >  2:5052639/1‑135 (MQ=255)

                   aTCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGtt                                                                                                                   >  1:3246987/1‑140 (MQ=255)

                        cGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTg                                                                                                              <  1:2238907/140‑1 (MQ=255)

                        cGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTg                                                                                                              >  1:2015507/1‑140 (MQ=255)

                              atgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACa                                                                                                        <  2:939553/140‑1 (MQ=255)

                                 aaaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCAAGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAGAAACCGTCTGTGTAGAAAAACCCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                     <  1:3118457/140‑1 (MQ=255)

                                    gTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCCCGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAACACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCa                                                                                                  <  2:1254369/140‑1 (MQ=255)

                                     tGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCAt                                                                                                 >  1:3963134/1‑140 (MQ=255)

                                        aaCACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCg                                                                                              <  2:2507808/140‑1 (MQ=255)

                                             ttACCCGTAGAAATGACGCTAACTTCGGCAACCACGTATGGCACACATCTGCTCAATACTTCCCACCAGGACTTGATAATCAGTCTGTGTATAAACAACCATACCCATCTTGTTATCCGCCAACAACGCCATCCGtagca                                                                                         >  2:4689544/1‑138 (MQ=255)

                                              tccccGTAGACATGACCCTATCTTCGGCAACCACGTATGGCAACCATTTGCCCAATACTTCCCGCCAGGACTTGAAACACCGTCTGTGTAGAAACAACCATACCTATGGTCTTATCTGCCCACAACGCCATCCGGACAAg                                                                                        <  2:2983981/138‑1 (MQ=255)

                                                    tAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                                  >  1:1814170/1‑140 (MQ=255)

                                                    tAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                                  >  2:852158/1‑140 (MQ=255)

                                                     aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATg                                                                                 >  1:1302241/1‑140 (MQ=255)

                                                     aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATg                                                                                 >  1:2294714/1‑140 (MQ=255)

                                                      gAAATGACGGTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGCAAACCCGTCTGTGTAGAAAAGACCATAGCTCTGTTGTGATCTGCCGACAACGCCATCCGGAGAAGCCGACTGa                                                                                <  2:1710231/140‑1 (MQ=255)

                                                      gAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                                                >  2:3478861/1‑140 (MQ=255)

                                                       aaaTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGAc                                                                               <  2:4772677/140‑1 (MQ=255)

                                                           gACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGGAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTg                                                                           >  1:4713878/1‑140 (MQ=255)

                                                           gACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTg                                                                           >  2:2434699/1‑140 (MQ=255)

                                                           gACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTg                                                                           >  2:2087078/1‑140 (MQ=255)

                                                           gACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTg                                                                           >  2:2945440/1‑140 (MQ=255)

                                                             cGCTATCTTCGGCAACCACGTATGGCAAACGCTTGCGCACTACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTc                                                                         <  2:2837837/140‑1 (MQ=255)

                                                                 aTCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAg                                                                     >  1:1571129/1‑140 (MQ=255)

                                                                  tCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGt                                                                    >  1:1194957/1‑140 (MQ=255)

                                                                      cGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                                >  1:822218/1‑140 (MQ=255)

                                                                      cGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                                <  1:1599380/140‑1 (MQ=255)

                                                                      cGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                                <  2:4906190/140‑1 (MQ=255)

                                                                       ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcg                                                               <  1:492073/140‑1 (MQ=255)

                                                                       ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcg                                                               >  1:138690/1‑140 (MQ=255)

                                                                        gCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcgc                                                              <  2:4313667/140‑1 (MQ=255)

                                                                        gCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcgc                                                              <  1:703125/140‑1 (MQ=255)

                                                                        gCAACCACGTAGGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGGAGCCGAATTACGTTGTCGTGGTAAgcgc                                                              >  2:3976057/1‑140 (MQ=255)

                                                                            ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGCCTTGAAAAACCGTCTGTGTAGGAAAAACCATAGCTATGGTGTTATCTGCCGATAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGt                                                          >  2:4786874/1‑140 (MQ=255)

                                                                            ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                                >  2:4636611/1‑134 (MQ=255)

                                                                            ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                                <  1:4636611/134‑1 (MQ=255)

                                                                            ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGt                                                          >  2:544804/1‑140 (MQ=255)

                                                                            ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGt                                                          >  1:2048109/1‑140 (MQ=255)

                                                                            ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGCAGTAAGCGCTGGt                                                          >  2:4465340/1‑140 (MQ=255)

                                                                             cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  2:5195348/140‑1 (MQ=255)

                                                                             cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  2:3860296/140‑1 (MQ=255)

                                                                             cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  2:1150887/140‑1 (MQ=255)

                                                                             cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  2:2344927/140‑1 (MQ=255)

                                                                             cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  2:182447/140‑1 (MQ=255)

                                                                             cACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                         <  1:3803262/140‑1 (MQ=255)

                                                                             cACGTATGGCAAACATGTGCGCAATACTACCCACCAGGACCTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTCTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACCTTGTCGGAGTAAGCGCTGGtc                                                         <  2:4317235/140‑2 (MQ=255)

                                                                              aCGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTa                                                        >  1:1443900/1‑140 (MQ=255)

                                                                                   tGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGgtg                                                   >  2:4980236/1‑140 (MQ=255)

                                                                                      cAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTgg                                                >  1:3166528/1‑140 (MQ=255)

                                                                                      cAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTgg                                                >  1:771425/1‑140 (MQ=255)

                                                                                      cAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTgg                                                >  1:2501021/1‑140 (MQ=255)

                                                                                        aaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGt                                              <  2:1515754/140‑1 (MQ=255)

                                                                                         aCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcg                                                               <  1:2864241/122‑1 (MQ=255)

                                                                                         aCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGtt                                             >  2:3683027/1‑140 (MQ=255)

                                                                                         aCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTCTAGAAAAACCCATAGCTATGGTGTCATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACATTGTAGGAGTAAGCGCTGGTTAAGGTGTGGGtt                                             >  2:1992376/1‑140 (MQ=255)

                                                                                          cATTTGCGCAATACTTCCCGCCTGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtg                                            >  2:5024088/1‑140 (MQ=255)

                                                                                          aaTTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtg                                            <  2:4885329/139‑1 (MQ=255)

                                                                                             ttGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                                         <  2:1110487/140‑1 (MQ=255)

                                                                                              tGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGcc                                        >  1:212542/1‑140 (MQ=255)

                                                                                                  cAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                                    <  1:1099517/140‑1 (MQ=255)

                                                                                                  cAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGGTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                                    <  1:2842608/140‑1 (MQ=255)

                                                                                                    aTACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTg                                  >  2:3618023/1‑140 (MQ=255)

                                                                                                     tACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGtt                                                                                                                   >  1:3555055/1‑58 (MQ=255)

                                                                                                       cTTCCCTCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCAGAGCTATGGTGTTATCGGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGtt                               <  2:2484251/140‑1 (MQ=255)

                                                                                                         tCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGa                             <  2:941658/140‑1 (MQ=255)

                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAg                            >  1:3863351/1‑140 (MQ=255)

                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAg                            >  1:4538155/1‑140 (MQ=255)

                                                                                                             gacAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCAGCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTGAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGt                         <  1:408829/138‑1 (MQ=255)

                                                                                                             gCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGt                         <  1:408564/140‑1 (MQ=255)

                                                                                                             gCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGGGGGTTGTGCCTCTTTGGTTGAGGGt                         >  1:2153633/1‑140 (MQ=255)

                                                                                                               cAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTg                       >  2:2616668/1‑140 (MQ=255)

                                                                                                                 ggACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                                         <  1:3154947/120‑1 (MQ=255)

                                                                                                                  gACTTGAAATACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGATGTTGGCGTAGTAAGCGCGGGCTACGGTGTGGTGGGGGCCTCTTTGGTTGAGGGTTGcgt                    >  2:3177144/1‑140 (MQ=255)

                                                                                                                        aaaaaCCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGc              <  1:3756788/140‑1 (MQ=255)

                                                                                                                           aaCCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGa           <  2:4538155/140‑1 (MQ=255)

                                                                                                                               gTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTa       >  1:3323070/1‑140 (MQ=255)

                                                                                                                               gTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTa       >  1:1889087/1‑140 (MQ=255)

ATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGT  >  NZ_CP025268/1252562‑1252833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`