BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,001	A→G	noncoding (36/132 nt)	CXP41_RS06835 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,001	0	A	G	99.6%	702.4 / NA	258	noncoding (36/132 nt)	CXP41_RS06835	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (111/144); total (113/145)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

TTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTT  >  NZ_CP025268/1292865‑1293126

 tGCGTATTCGTTTGAGAATTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                           <  2:3987886/140‑1 (MQ=255)

 tGCGGATTCGTTTGTGAATTCTTGGGCTTTTGCTAGTGATGGTGGTGGGGGAAGGATTACTCGGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGa                                                                                                                           <  2:432338/140‑1 (MQ=11)

 tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                           >  1:1037899/1‑140 (MQ=255)

 tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                           <  1:4423058/140‑1 (MQ=255)

 tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                           >  2:2958116/1‑140 (MQ=255)

 tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                           <  2:5007307/140‑1 (MQ=255)

 tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                           <  2:2635551/140‑1 (MQ=255)

 tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                           >  2:2518668/1‑140 (MQ=255)

 tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTAGTCGa                                                                                                                           >  2:220963/1‑140 (MQ=14)

   cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGACCCTTAGTCGAAg                                                                                                                         >  1:36905/1‑140 (MQ=255)

   cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAg                                                                                                                         >  2:2909824/1‑140 (MQ=255)

   cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                         >  2:730961/1‑140 (MQ=255)

     gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTGGTCGAAGCt                                                                                                                       >  1:3076261/1‑140 (MQ=255)

     gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                                       >  1:3076352/1‑140 (MQ=255)

           tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                 <  1:3777366/139‑1 (MQ=255)

            ttGAGAATTCCGTGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATc                                                                                                                <  2:824/140‑1 (MQ=255)

            ttGAGAATTCCGGGGCTTTTGAATGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATc                                                                                                                <  1:2610609/140‑1 (MQ=255)

                ggaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                            <  2:4974990/138‑1 (MQ=255)

                gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                            <  1:3799868/140‑1 (MQ=255)

                  tttCCGGGGCTTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                          <  2:466214/139‑1 (MQ=255)

                  aTTTCGGTGCGTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTGCGCCCTTCGGGTCGTTGCCTGCGGCCGCGCTCTCTCGCTGACGCGCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                          <  2:4417917/140‑1 (MQ=11)

                    tCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCACCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCg                                                                                                        >  1:3151164/1‑140 (MQ=255)

                     ccGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTTGCCCTTCGGGTCGTTGCCTGTGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAAGCTTGGTCGATGCTTCTCAGCCTTCCCCGc                                                                                                       >  2:1748221/1‑140 (MQ=11)

                      cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                      <  1:485757/140‑1 (MQ=25)

                       ggggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                     <  1:3279501/140‑1 (MQ=37)

                          gtttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                  <  2:4855750/138‑1 (MQ=17)

                          ggtcttGAATGTGATGGTGGGCGTGGCAGCATTACTCAGTGCTGCGCCCTTCGCCCTTCGGGTCGTTGCCCGCGGCACCGCTCCGTCGCTGACCCCCGAGCGGCACCGTAGTCGGAGGGTCTCATCCTTCCCCGCATggg                                                                                                  <  2:3933565/136‑1 (MQ=2)

                          gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                  <  2:1882766/140‑1 (MQ=18)

                          gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                  <  1:3563226/140‑1 (MQ=18)

                          gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                  <  2:1925626/140‑1 (MQ=18)

                          gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                  <  1:4930273/140‑1 (MQ=18)

                          gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                  <  1:806929/140‑1 (MQ=18)

                          gCTTTAGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                  <  2:5123858/140‑1 (MQ=255)

                            ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                <  1:5011413/140‑1 (MQ=18)

                                     tGATGGGGGTGGTGGAAGGATTACTCAGCGTTGCGCGCTTCGCCCTTCGGGCCGTTGCCTTTGGCAACGTTCTCTCGCTGTCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCACTCTTCCCCGTATGGGGAGAATTTTTa                                                                                       >  2:1439947/1‑140 (MQ=11)

                                      gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTACCCCCATGGGCAGTATAttttt                                                                                      >  2:2875760/1‑138 (MQ=11)

                                               gggggAAGGATTACTCAGTGCTGCGCGCTTCCCTGTTCGGGTCGTTGCCCGCGGCACAGCTCTGTCGCTTACGCTCGGGCCCAACCTGAGTCGAAGCTTCTTATCCTGCCACGCAGGGGCAGAATATTTAATTGCGGagt                                                                             >  2:4546766/1‑138 (MQ=11)

                                               gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAtt                                                                             >  2:314612/1‑140 (MQ=25)

                                                    aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                        >  1:2420119/1‑140 (MQ=11)

                                                     aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                       >  1:1378037/1‑140 (MQ=11)

                                                      ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                      <  2:4217591/140‑1 (MQ=11)

                                                       ggttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAGTGCGGATTCGTTGGGa                                                                     >  1:4160992/3‑140 (MQ=11)

                                                       gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                     <  1:888812/140‑1 (MQ=11)

                                                            cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTc                                                                >  1:4374280/1‑140 (MQ=14)

                                                            cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGCATATTTAATTGCGGATTCGCTggcgaggtc                                                                >  2:4828841/1‑133 (MQ=11)

                                                            cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGAAACGCTCTCTCGCTGGCGCTCGCGTCGCACCTTTGTCGAACCTTCTCATCCTTCCCCGCATGGGCAGACTATGTCATTGCGGATTCGTTGGGAAggtg                                                                >  2:955285/1‑137 (MQ=11)

                                                               aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                             >  1:4727566/1‑140 (MQ=14)

                                                               aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                             >  1:3681875/1‑140 (MQ=14)

                                                                gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                            <  1:562337/140‑1 (MQ=14)

                                                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                           >  1:5103988/1‑140 (MQ=14)

                                                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTCAGGGa                                                           >  1:4222014/1‑140 (MQ=14)

                                                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAAAATCTTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                           >  2:1556896/1‑140 (MQ=14)

                                                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                            >  1:2094454/1‑139 (MQ=14)

                                                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                            <  2:2094454/139‑1 (MQ=14)

                                                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                                  >  2:3342770/1‑85 (MQ=25)

                                                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                                  <  1:3342770/85‑1 (MQ=25)

                                                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                      <  1:2835913/140‑1 (MQ=255)

                                                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                      >  1:4807635/1‑140 (MQ=255)

                                                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                      >  1:3971293/1‑140 (MQ=14)

                                                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAGTTAATTGCGGAATCGTTTGGAAATTCAGGGACtttt                                                      >  2:3514884/1‑140 (MQ=11)

                                                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                              <  1:2227922/100‑1 (MQ=21)

                                                                       gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                     <  1:3998679/140‑1 (MQ=14)

                                                                       gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                     <  1:3998425/140‑1 (MQ=255)

                                                                        cgcTTCGCCCTTCGGATCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGa                                                    <  2:4144803/140‑1 (MQ=14)

                                                                         gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                   <  1:2005557/140‑1 (MQ=14)

                                                                           ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTTGGACGTTCAGGGACTTTTGaaaa                                                 >  2:277752/1‑139 (MQ=11)

                                                                           ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                 >  1:1267008/1‑140 (MQ=14)

                                                                             cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                            <  1:1646160/127‑1 (MQ=14)

                                                                             cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                            >  2:1646160/1‑127 (MQ=14)

                                                                               cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGTGTGGGCAGAATATTTAATTGCGGATTCGTTGGGAGGTTCAGGGACTTTTGAAAGTGAT                                             <  2:2594188‑M1/140‑1 (MQ=255)

                                                                                ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATG                                            <  1:2295134‑M1/140‑1 (MQ=255)

                                                                                ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATG                                            <  1:4621350‑M1/140‑1 (MQ=255)

                                                                                 cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGATAGTGATGG                                           <  2:3115283‑M1/140‑1 (MQ=255)

                                                                                   tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTG                                         <  1:1593985‑M1/140‑1 (MQ=255)

                                                                                    cGGTTCGTTCCCTCCGGCAACCCTATCTCGCTGGCCCTCGAGTCGCTCCATCGTCGAAGCTTCACATCCTCCCCCGCATGGGCACAATATTTAATTGCGGATTCGTCGCGAAGTTCAGGGACTTTTGAAAGCGAtggtgg                                        <  2:13819/140‑1 (MQ=9)

                                                                                    cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAGCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGG                                        <  1:1170220‑M1/140‑1 (MQ=255)

                                                                                    cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGG                                        <  1:3827285‑M1/140‑1 (MQ=255)

                                                                                      gggcgttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGGCTCAGCCTTCCCCGCATGGGCAGAAGATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTG                                      <  2:3076261‑M1/137‑1 (MQ=255)

                                                                                      ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTG                                      <  1:1737465‑M1/140‑1 (MQ=255)

                                                                                      ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTG                                      <  2:3681875‑M1/140‑1 (MQ=255)

                                                                                      ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTG                                      <  1:2454030‑M1/140‑1 (MQ=255)

                                                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGG                                  >  2:3405846‑M1/1‑140 (MQ=255)

                                                                                           ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGCGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                 <  1:1760765‑M1/140‑1 (MQ=255)

                                                                                           ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                 <  2:4007550‑M1/140‑1 (MQ=255)

                                                                                           ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                 <  2:3303895‑M1/140‑1 (MQ=255)

                                                                                           ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                 <  1:2926721‑M1/140‑1 (MQ=255)

                                                                                           ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                 <  1:220963‑M1/140‑1 (MQ=255)

                                                                                            tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                >  1:4554756‑M1/1‑140 (MQ=255)

                                                                                            tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGGGATGGTGGTGGGGGAA                                >  1:269422‑M1/1‑140 (MQ=255)

                                                                                            tGCCTGCGGCAACGCGCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGGCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                >  2:41845‑M1/1‑140 (MQ=255)

                                                                                               cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                   <  1:903497/118‑1 (MQ=14)

                                                                                                   ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcg                         <  2:628753‑M1/140‑3 (MQ=255)

                                                                                                           tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcg                 <  1:4505844‑M1/140‑11 (MQ=255)

                                                                                                              ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaag              <  2:3738564‑M1/140‑14 (MQ=255)

                                                                                                                  ctggcgctcGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcga          >  2:623355‑M1/5‑123 (MQ=255)

                                                                                                                        cTCGGGTCGAACCTTGGTCGAAGATTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg    <  2:3427024‑M1/140‑24 (MQ=255)

                                                                                                                        cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg    <  1:1571789‑M1/140‑24 (MQ=255)

                                                                                                                        cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg    <  1:2925111‑M1/140‑24 (MQ=255)

                                                                                                                          cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggca  <  1:3646066‑M1/140‑26 (MQ=255)

                                                                                                                          cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggca  <  1:706565‑M1/140‑26 (MQ=255)

TTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTT  >  NZ_CP025268/1292865‑1293126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`